Made available in DSpace on 2014-12-17T15:36:58Z (GMT). No. of bitstreams: 1 RovenaCGJE_DISSERT.pdf: 2142357 bytes, checksum: bbf33b27b7a838600336db16673db325 (MD5) Previous issue date: 2010-04-23 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The parabrachial complex (PB) is an area of the brainstem responsible for the processing and transmission of essential physiologic information for the survival of the organisms. This region is subdivided in approximately nine subregions, considering morphology, cytoarchitectural and functional characteristic. Its neurons have an extensive network of connections with other regions of the nervous system. The objective in this work was to map the retinal projection to the PB and make a citoarchitectonic and neurochemical characterization of this region in the common marmoset (Callithrix jacchus), a primate of the New World. The retinal projections were mapped by anterograde transport of the choleric toxin subunit b (CTb). The citoarchitecture was described through the Nissl method, and the neurochemical characterization was made through immunohistochemical technique to the some neurotransmitters and neuroactives substances present in this neural center. In marmoset PB, in the coronal sections labeled by Nissl method, we found a similar pattern to that evidenced in other animal species. The immunoreactivity against CTb was verified in the PBMv in fibers/terminal, characterizing such as retinal innervations in this area. The immunohistochemical technique reveled that the PB contain cells, fibers and/or terminals immunoreactives to the neuronal nuclear protein, Choline acetyl transferase, nitric oxide synthase, serotonin, enkephalin, substance P, Calcium-binding proteins (calbindin, calretinin e parvalbumin), and glial fibrillary acidic protein. The histochemical technique reveled cells and fibers NADPH-diaphorase reactive. Each one of those substances presented a characteristic pattern of demarcation in PB, and some serve as specific markers of subregions / O complexo parabraquial (PB) ? uma regi?o do tronco encef?lico respons?vel pelo processamento e transmiss?o de informa??es fisiol?gicas essenciais para a sobreviv?ncia dos organismos. Essa regi?o ? subdividida em aproximadamente nove regi?es, considerando caracter?sticas morfol?gicas, citoarquitet?nicas e funcionais. Seus neur?nios possuem uma ampla rede de conex?es com as demais regi?es do sistema nervoso. O objetivo deste trabalho foi mapear a proje??o retiniana para o PB e fazer uma caracteriza??o citoarquitet?nica e neuroqu?mica desta regi?o no Callithrix jacchus (sag?i), um primata do Novo Mundo. As proje??es retinianas foram mapeadas por transporte anter?grado da subunidade B da toxina col?rica (CTb). A citoarquitetura foi descrita atrav?s do m?todo de Nissl e a caracteriza??o neuroqu?mica foi feita atrav?s de t?cnicas imunoistoqu?micas para alguns neurotransmissores e subst?ncias neuroativas presentes neste centro neural. No PB do sagui, nas sec??es coronais coradas pelo m?todo de Nissl, foi poss?vel encontrar um padr?o similar ao que ? evidenciado em outras esp?cies animais. A imunorreatividade contra CTb foi encontrada em fibras terminais do PBMv, caracterizando desta forma uma inerva??o retiniana nessa ?rea. A t?cnica imunoistoqu?micas revelou que o PB cont?m c?lulas, fibras e/ou terminais imunorreativos a prote?na nuclear neuronal, colina acetiltransferase, ?xido n?trico sintetase, serotonina, encefalina, subst?ncia P, prote?nas ligantes de c?lcio (calbindina, calretinina e parvalbumina), e a prote?na ac?dica fibrilar glial. A partir de t?cnicas histoqu?micas verificou-se c?lulas e fibras reativas a NADPH-diaforase. Cada uma dessas subst?ncias apresentou um padr?o caracter?stico de marca??o no PB e algumas serviram como marcadores espec?ficos de subregi?es