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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Morfologia de ovos, larvas e adultos de Paratanaisia bragai (Santos, 1934) Freitas, 1959 (Digenea, Eucotylidae) e histopatologia do rim de Columba livia (Gm.) infectado / Morphology of eggs, larvae and adult of Paratanaisia bragai (Santos, 1934) Freitas, 1959 (Digenea, Eucotylidae) and histopathology of the kidney Columba livia infected

XAVIER, Vanessa Barreto 24 February 2015 (has links)
Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-06-06T20:04:45Z No. of bitstreams: 1 2015 - Vanessa Barreto Xavier.pdf: 10480934 bytes, checksum: dcefb748f49bc38601b8bbc19c70ebc8 (MD5) / Made available in DSpace on 2017-06-06T20:04:45Z (GMT). No. of bitstreams: 1 2015 - Vanessa Barreto Xavier.pdf: 10480934 bytes, checksum: dcefb748f49bc38601b8bbc19c70ebc8 (MD5) Previous issue date: 2015-02-24 / CAPES / Paratanaisia bragai is a digenetic that reaches sexual maturity in the collecting ducts of domestic and wild birds, and larval development using the snail Subulina octona or Leptinaria unilamellata. The embryonated eggs are released with the waste products of the definitive host and the infection settles in the snail by ingestion of these. After hatching, the miracidium, develop inside the snail two sporocysts generations, cercariae and metacercariae. The definitive host acquires infection by ingestion of parasitized snail. The present study was aimed to identify the action of the parasite on the kidney of pigeons, Columba livia, through histology, and the morphometry, morphology and ultrastructure using light microscopy, scanning electron and transmission of the egg, larval stages (miracidium , cercariae and metacercariae) and adult helminth P. bragai. The adults pigeons were obtained near the Central de Abastecimento do Estado do Rio de Janeiro S.A, Iraj?, Munic?pio do Rio de Janeiro. After parasitological examination in the laboratory, to check infection, the pigeons infected were necropsied to collect helminth and infected kidney. The pigeons were used as uninfected controls. The experimental protocols were approved by the ethics committee on research UFRRJ. Histopathology showed dilatation of the renal tubules, with mononuclear inflammatory cells, formation of papilliform structure projecting into the tubular lumen and metaplasia of the epithelium of the collecting tubule walls, from simple cubic in the tubules uninfected to pseudostratified in infected kidney with P. bragai. The eggs are elliptical, with operculum at the anterior end and a knot at the posterior end, abopercular region. The eggshell is rough and composed of three layers: inner, middle and outer with thicknesses and different electrondense. The miracidium is elongated, with terebratorium the anterior end and body covered with cilia. The cercariae have cylindrical body that tapers slightly in the posterior region. The tegument is rough, the oral sucker is subterminal, the acetabulum stands in the middle third of the body. Papillae were observed around the oral sucker. A similar rudimentary tail structure was observed in some cercariae. The metacercariae observed through histological sections visualized the oral sucker, acetabulum, scales and cyst that consist of three layers, inner, middle and outer, of different thicknesses. The presence of the layers was confirmed by visualization of histological sections of metacercariae in scanning electron microscopy. The adult parasite is elongated and flattened body with oral sucker, pharynx, vitelline glands extending to the region of cecal bifurcation and then the median region of the body. The genital pore visualized a structure that is everted the cirrus in rosette form. In the adult stage the tegument is covered with scales of various types; simple scales, with two, three, four, five and seven divisions, a characteristic that may contribute to ratify the taxonomic classification of the parasite. / Paratanaisia bragai ? um digen?tico que atinge a maturidade sexual nos ductos coletores de aves dom?sticas e silvestres, e para o desenvolvimento larval utiliza o molusco Subulina octona ou Leptinaria unilamellata. Os ovos embrionados s?o liberados com os produtos de excre??o do hospedeiro definitivo e a infec??o no molusco se estabelece pela ingest?o destes. Ap?s a eclos?o do mirac?dio, desenvolvem-se no interior do molusco duas gera??es de esporocistos, cerc?rias e metacerc?rias. O hospedeiro definitivo adquire a infec??o por ingest?o do molusco parasitado. O presente estudo teve como objetivos: verificar a a??o do parasito sobre o rim de pombos, Columba livia, atrav?s da an?lise histopatol?gica bem como analisar a morfometria, morfologia e ultraestrutura utilizando a microscopia de luz, eletr?nica de varredura e transmiss?o do ovo, est?gios larvais (mirac?dio, cerc?ria e metacerc?ria) e helminto adulto de P. bragai. Pombos adultos foram obtidos pr?ximos a Central de Abastecimento do Estado do Rio de Janeiro S.A, Iraj?, Munic?pio Rio de Janeiro. Ap?s exame coproparasitol?gico, no laborat?rio, para verifica??o da infec??o, os pombos infectados foram necropsiados para a coleta dos helmintos e do rim infectado. Os pombos n?o infectados foram utilizados como grupo controle. Os protocolos experimentais foram aprovados pela comiss?o de ?tica na pesquisa da UFRRJ. A an?lise histopatol?gica revelou dilata??o dos t?bulos renais, processo inflamat?rio com c?lulas mononucleadas, forma??o de estrutura papiliforme projetando-se para a luz tubular e metaplasia do epit?lio da parede do t?bulo coletor, de epit?lio c?bico simples nos t?bulos n?o infectados a pseudroestratificado nos rins infectados com P. bragai. Os ovos s?o el?pticos, com op?rculo na extremidade anterior e n? na extremidade posterior, regi?o abopercular. A casca apresenta-se ?spera e composta por tr?s camadas: interna, m?dia e externa de espessuras e eletrondensidades diferentes. O mirac?dio ? alongado, com terebratorium na extremidade anterior e corpo coberto por c?lios. As cerc?rias apresentam corpo cil?ndrico que se afunila ligeiramente na regi?o posterior. O tegumento ? rugoso, a ventosa oral ? subterminal, o acet?bulo destaca-se no ter?o m?dio do corpo. Papilas foram verificadas ao redor da ventosa oral. Uma estrutura semelhante ? cauda rudimentar foi observada em algumas cerc?rias. Nas metacerc?rias observadas atrav?s de cortes histol?gicos visualizou-se a ventosa oral, o acet?bulo, as escamas e cisto composto por tr?s camadas, interna, m?dia e externa, de espessuras diferentes. A presen?a das camadas foi confirmada na visualiza??o dos cortes histol?gicos das metacerc?rias na microscopia eletr?nica de varredura. O parasito adulto tem corpo alongado e achatado com ventosa oral, faringe, gl?ndulas vitelog?nicas extracecais que se prolongam anteriormente at? a regi?o de bifurca??o cecal e posteriormente a regi?o mediana do corpo. No poro genital visualiza-se uma estrutura evertida que ? o cirro em formato de roseta. No est?gio adulto o tegumento ? coberto por escamas de v?rios tipos; escamas simples, b?fidas, tr?fidas, com quatro, cinco e sete divis?es, caracter?stica que pode contribuir para ratificar a classifica??o taxon?mica do parasito.

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