The objective of this study was to determine whether the composite bond strength acquired using a single step self-etching bonding system is as or more effective when compared to the two step etch-rinse-bond system at clinically relevant in-vitro standards. A total of 80 extracted human deciduous with at least one intact smooth surface were collected with parental consent. Collected teeth were randomly assigned to one of four treatment protocols (n=20): single step self-etch (Adper Prompt L-Pop, 3M ESPE), two step etch (15s)-rinse-bond (Optibond Solo Plus, Kerr), single step self etch (Adper L Pop, 3M ESPE) with 30s pre-etch, and two step etch (30s)-rinse-bond (Optibond Solo Plus, Kerr). A composite cylinder of uniform shape was bonded to each tooth using the designated adhesive system and specimens were tested with a universal testing machine. Descriptive statistics and statistical analysis was performed. Median shear bond strengths for all specimens, regardless of treatment group, ranged from 3.25 MPa to 43.13 MPa. These values are above suggested minimum clinical required values of 3MPa. A Kruskal-Wallis analysis between treatment groups found statistical significance (p<0.05) for shear bond strength between the groups and both one step system protocols, had higher mean and median shear bond strength values than the two step systems. An adhesive remnant index (ARI) was analyzed to confirm uniform adhesive fracture between all four treatment groups. Both a Fisher’s exact test, and a Hantel-Haenszel statistic found no significant difference (p<0.05) between the samples. Based on these findings we concluded that: 1. The ARI score for all treatment groups was not statistically significant, suggesting that the type of bond breakage was consistent regardless of adhesive system used. 2. Both Optibond Solo Plus and Adper Prompt L-Pop systems achieve adequate shear bond strengths as described by current literature. 3. The use of a one-step system to save chair time and aid in patient behavior management may be clinically useful.

Thesis (M.S.)--University of Michigan, 2005. / Includes bibliographical references.

Thesis (M.S.)--University of Michigan, 2005. / Includes bibliographical references.

FundaÃÃo de Amparo à Pesquisa do Estado do Cearà / O sucesso do tratamento endodÃntico depende de muitos fatores, sendo a reduÃÃo ou eliminaÃÃo da infecÃÃo bacteriana o mais importante desses fatores. Portanto, o uso de substÃncias capazes de agir nesses microorganismos e em seus subprodutos torna-se uma etapa importantÃssima no tratamento. O objetivo deste ensaio clÃnico âsplit-mouthâ foi comparar a eficÃcia da clorexidina gel 1% e do hidrÃxido de cÃlcio associado ao paramonoclorofenol canforado (Callen PMCCÂ), como medicaÃÃo intracanal, e do tratamento executado em sessÃo Ãnica (grupo controle), contra Estreptococos do grupo mutans (EGM) e bactÃrias anaerÃbias presentes no interior dos canais radiculares de molares decÃduos com necrose pulpar. Um total de 21 crianÃas (37 dentes) participou do estudo. Amostras iniciais (prÃ-tratamento) e finais (apÃs a permanÃncia das substÃncias por 14 dias no interior dos canais) foram coletadas para anÃlise microbiolÃgica e incubadas em placas de Mitis Salivarius Bacitracina, em aerofilia, para a observaÃÃo dos nÃveis de EGM, assim como em placas de Brucella Ãgar, em anaerobiose, para verificaÃÃo de bactÃrias anaerÃbias. Os nÃveis de sucesso da pulpectomia apÃs um perÃodo de acompanhamento de atà 12 meses foram analisados baseados em parÃmetros clÃnicos e radiogrÃficos. A clorexidina gel a 1% reduziu significantemente os nÃveis de EGM (p= 0,010, teste de Wilcoxon) e o Callen PMCC reduziu significantemente os nÃveis de bactÃrias anaerÃbias (p=0,002, teste de Wilcoxon). Observou-se diferenÃa significativa na comparaÃÃo da reduÃÃo dos nÃveis de EGM obtidos pelo grupo da clorexidina e pelo controle (p=0,032, Mann-Whitney). A taxa de sucesso do tratamento foi de 81,71% no grupo do Callen PMCCÂ, 78,57% para o grupo da clorexidina gel 1% e 77,77% no grupo controle. Concluiu-se que a clorexidina gel a 1%, assim como o hidrÃxido de cÃlcio associado ao paramonoclorofenol canforado, possui eficÃcia limitada na reduÃÃo de bactÃrias dos canais radiculares decÃduos infectados. Os presentes resultados sugerem que uma possÃvel associaÃÃo entre as medicaÃÃes testadas em estudos futuros pode eliminar de maneira mais eficaz estas bactÃrias. / The success of endodontic treatment depends on many factors, and the reduction or elimination of bacterial infection is the most important one. Therefore, the use of substances that act against these microorganisms and their products becomes an important stage in treatment. The aim of this in vivo split-mouth study was to compare the efficacy of a 1% chlorhexidine gel, calcium hydroxide/camphorated paramonochlorophenol (Callen PMCCÂ) as intracanal medications, and a Control group (1-visit endodontic treatment) against mutans streptococci (EGM) and anaerobic bacteria found in primary molars with necrotic pulps. A total of 21 children (37 teeth) participated in this study. Initial (pre-treatment) and final (14 days post-treatment) intra-canal samples were collected for microbiological analysis and were incubated in Mitis Salivarius Bacitracin plates under microaerophilic conditions for EGM counting; as well as in Brucella-agar plates, anaerobically, to allow growth of anaerobic bacteria. The success rate of the pulpectomies after a 12 months follow-up were also evaluated based on clinical and radiographic parameters. Chlorhexidine gel significantly reduced EGM levels (p=0,010, Wilcoxon test), whereas Callen PMCC significantly reduced anaerobic bacteria levels (p=0,002). Significant difference was observed when comparing EGM reduction levels between the Chlorhexidine and Control groups (p=0,032, Mann-Whitney test). The success rate was 81,71% in the Callen PMCC group, 78.57% in the Chlorhexidine group and 77.77% in the control group. We concluded that 1% chlorhexidine gel, as well as calcium hydroxide/camphorated paramonochlorophenol, has limited efficacy in the reduction of bacteria from deciduous infected root canals. The present results suggest that a possible association between these two medications in future studies may eliminate more efficiently these bacteria.

No description available.

Indiana University-Purdue University Indianapolis (IUPUI) / Dietary recommendations concerning chocolate milk remain controversial since the effect of chocolate milk on the dental caries process is not clear. Cocoa with antibacterial and enamel-solubility-reducing properties may inhibit the formation of dental caries. Since chocolate milk contains a significant amount of sucrose (about 5 percent) and some cocoa (1 percent), laboratory testing of the cariogenicity of chocolate milk seems valuable. The present study investigated whether or not under mouth simulation conditions chocolate milk influenced the formation of dental caries compared to white milk. A control solution, four milk solutions and a milk solution with toothbrushing were tested over a 20-week experimental period. A mouth-like environment was established by constructing a mouth simulating device. One-hundred- and-sixty-two-teeth were mounted in the mouth simulator in six groups of 27 teeth each. Two independent evaluators had certified the teeth to be caries-free and a computer program was used to ensure complete randomization of the teeth in groups. After initial sterilization by ethylene oxide, the teeth were inoculated with a mixture of a culture of Streptococcus mutans and saliva. Each group was exposed to one of the milk formulations for a 15 minute period twice daily. After each period, a sterile bacterial medium was dripped (8 to 12 mls/hr) over the teeth in the mouth-like environment. After 20 weeks the teeth were separated, coded, and re-evaluated for pit and fissure caries by the same two evaluators. A statistical analysis by Repeated t Tests indicated the presence of three levels of relative cariogenicity: the chocolate milk group had the highest caries rate, the control group and the white milk group were intermediate and the chocolate milk with brushing group showed a marked reduction in dental caries. The results of two other groups were invalidated. In summary, for pit and fissure dental caries under the conditions tested in the mouth simulating device, chocolate milk exhibited a significant cariogenic potential relative to white milk, especially in the early incipient caries stage. It may be concluded from this study that in an individual with high dental caries susceptibility, it would seem unwise to recommend frequent ingestion of chocolate milk, unless proper and immediate oral hygiene follows the ingestion.

Indiana University-Purdue University Indianapolis (IUPUI) / The purpose of this study was to test the relationship between dental developmental age and chronologic age as they relate to other aspects of the child's development. The dental developmental age was determined on 74 children, 40 males and 34 females, ranging in age from two to 11 years. The sample population was chosen at random from patients at the Indiana University School of Dentistry. The socio-economic status was determined according to the North-Hatt Occupational Scale; 14.3 per cent of the children fell in the upper class, 84.3 per cent in the middle class, and 1.4 per cent in the lower class. Analysis by race showed that 71.6 per cent of the sample were Caucasian, 23.0 per cent were Negroid, and 5.4 per cent were Oriental. Panoramic radiography, with the S.S. White Panorex, was used to evaluate dental developmental age. Two independent observers scored the radiographs and double blind procedures were used. To determine dental developmental age, Wolanski's method of tooth formation evaluation was used. To determine functioning of children in five developmental skills areas, the Alpern-Boll Developmental Skills Inventory was used. Dental developmental age and chronologic age had a significant positive relationship to children's functioning in five developmental skills areas. There is a chronologic period when determination of dental age appears to be difficult. The data available and methodology for determining dental developmental age of the four-year-old female and the five-year-old male seems to be inadequate. Dental developmental age seems to be a better predictor of general developmental skills for males than it does for females, specifically in the two to eight-year-old group. The highest correlations were found in the youngest age group, i.e. the two to five-year-olds. The correlations between dental developmental age and chronologic age, and between those two indices and the five developmental skills ages remains highly significant in the younger ages but decreases consistently as the child becomes older. The specific age as well as the sex of the child has a definite effect on the correlations obtained. The use of dental developmental age is good but not superior to the use of chronologic age for predicting functioning for normal children. This may not be the case for atypical children. More study is indicated.

Indiana University-Purdue University Indianapolis (IUPUI)

No description available.

Purpose: The purpose of this study was to determine the relationship of the CYP2B6 genotype to the clinical response to meperidine in pediatric dental patients. Methods: Forty-nine patients, ASA I/ II, 41–101 months old, received an oral sedative regimen containing meperidine for dental treatment. The North Carolina Behavior Rating Scale (NCBRS) and Overall Effectiveness of Sedation Scale (OESS) were used to assess their behavior and sedation outcome. Saliva DNA samples were genotyped by PCR-RFLP. Results: We found the following genotype distributions: homozygous wild-type 1*1 (n = 19, 39%), heterozygous 1*6 (n = 25, 51%), and homozygous variant 6*6 (n = 5, 10%). The genotypes showed a significant difference in the North Carolina Behavior Rating Scores and a trend towards significance of the Overall Effectiveness of Sedation Scale during meperidine oral sedations. Conclusion: This research concludes that variations of the CYP2B6 enzyme can be used in the prediction of successful behaviors for oral sedations that include meperidine in the drug regimen. Future research regarding the enzyme kinetics of meperidine is needed to determine the exact enzymatic function of CYP2B6 and its variants.