Diss. (sammanfattning) Alnarp : Sveriges lantbruksuniv., 2001. / Härtill 5 uppsatser.

Sedan 80-talet har man i Sverige på försöksnivå anlagt små bestånd av masurbjörk, stora kunskapsluckor finns framförallt hur man säljer och vem man säljer virket till. Arbetet syftar till att beskriva marknaden för masurbjörk samt att beräkna virkesvärdet hos två bestånd i Västerbotten. Arbetet bygger på en marknadsundersökning och en fältstudie. I Sverige köper knivtillverkare masurbjörk årligen, det går även att sälja till finska virkesköpare. För de två undersökta bestånden i Västerbotten, 15 år gamla beräknades virkesvärde till ~9400 kr/Ha respektive ~7000kr/Ha. Om man sköter ett masurbjörksbestånd finns det stor chans att få hög avsättning för virket även i framtiden.

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Made available in DSpace on 2014-06-12T15:49:29Z (GMT). No. of bitstreams: 1 license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2008 / Um animal imunocomprometido tem dificuldade de se restabelecer depois de sofrer uma injúria. Lectina de sementes de Parkia pendula, PpeL, foi usada como tratamento de lesões cutâneas, com o objetivo de alcançar menores efeitos colaterais, considerando seu potencial cicatrizante. O metotrexato (0.8 mg/kg/semana) foi usado como uma droga imunossupressora. Uma ferida cirúrgica foi produzida (1 cm2) na região dorsal em camundongos albinos suíços (Mus musculus), fêmeas normais e imunossuprimidas. Feridas foram tratadas diariamente com administração tópica de 100 μL das seguintes soluções: Grupo 1 - NaCl 0.15 M; Grupo 2 - imunossuprimido NaCl 0.15 M; Grupo 3 - PpeL (100 μg/ mL) ; Grupo 4 - imunossuprimido PpeL (100 μg/ mL). Foram usados parâmetros clínicos tais como, edema, hiperemia, crosta, tecido de granulação e cicatricial, como também contrações das feridas analisadas durante 12 dias. Biópsias para a análise histopatológica e exames microbiológicos foram realizadas após 2º, 7º e 12º dias. Observou-se a presença de edema e hiperemia em todos os grupos durante o período inflamatório. A primeira crosta foi observada a partir do segundo dia somente nos grupos tratados com PpeL. A análise microbiológica das lesões revelou que nos dias 0 e 2, o grupo 3 não apresentou Staphylococcus sp., entretanto foi observada a presença da bactéria nos outros grupos todos os dias. O uso da lectina também diminuiu a área da ferida, ocorrendo reepitelização total dos grupos 3 e 4 no 11º dia de evolução, enquanto os grupos controles alcançaram o fechamento das lesões no 12º dia. O presente estudo sugere que PpeL é um potencial biomaterial que apresenta evidência farmacológica preliminar no processo de reparo de lesões cutâneas

Made available in DSpace on 2014-06-12T15:53:55Z (GMT). No. of bitstreams: 2 arquivo4871_1.pdf: 811276 bytes, checksum: ed8d875a744e4db6006122f8f20f8241 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2006 / A imobilização de proteínas em suportes insolúveis em água constitui-se em importante ferramenta biotecnológica. Por outro lado, a magnetização suporte sintetizado aumenta seu potencial de uso pela facilidade de sua remoção do meio de reação, mediante o uso de um campo magnético, permitindo a remoção de contaminantes e sua reutilização. Dentre as classes de proteínas imobilizáveis destacam-se as lectinas, como a Concanavalina A (Con A), com afinidade a resíduos de glucose, manose e derivados. Gomas provenientes de vegetais constituem uma ótima fonte de polissacarídeos. Sementes de Parkia pendula são capazes de produzir goma (PpeG). Deste modo, o presente trabalho teve como objetivo magnetizar a PpeG, propor como matriz para a imobilização de lectinas e purificar glicoconjugados. Sementes de P. pendula foram hidratadas e o sobrenadante foi submetido à filtração, precipitação, lavagem com etanol e secagem. A goma resultante foi magnetizada pelo método de co-precipitação em solução de cloretos férrico e ferroso, submetida a ativação com NaIO4, e finalmente, incubada com a ConA. A purificação da glicoproteína fetuína foi utilizada como modelo para investigar o uso do derivado magPpeG-ConA como matriz de afinidade. Após a cromatografia e eluição realizada com glucose (300mM), a presença de fetuína foi investigada através de SDS-PAGE. Cerca de 63% de Con A ofertada foi imobilizada no suporte. 14% da amostra de fetuína foi recuperada por eluição competitiva. Depreende-se desses resultados que a goma de P. pendula mostrou ser um polissacarídeo com potencial para utilização como suporte para imobilização de proteínas e sua aplicação como suporte em cromatografia de afinidade

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In this thesis was observed an influence of silvicultural treatment on the structure of young birch stand. On location Stará Ves nad Ondřejnicí was chosen a 10 years old birch stand and on location Hlubočec a 15 years old one. The stands originated spontaneously from natural regeneration on clearing. On both locations were researched two stands in 2015, 2016 and 2017. Within the stands, control variants and the thinning ones were established in 2015. In the case of variants focused on a thinning was executed a repeated intervention in 2016 and in the same time a pruning of a part of individuals in the spring of 2017. On all variants all the woody plants were recorded with a height above 2 metres, which were measured a diameter at breast height (cm) during the years 2015–2017. On intervention variants at marked trees (samplers) were measured a total tree height (cm), an occurrence of a dry treetop (cm) and an occurrence of a live treetop (cm) as well. At the beginning and at the end of 2017 was also measured a treetop projection (cm) at all individuals on the area. Non-interventional stands reported a high number of individuals with a low diameter at breast height in both cases, which represented a significantly low resistence of these stands. This led to a reduction in stability, especially against the snow. Despite a major reduction in the number of individuals in the intervention stands, the proportion of trees was still higher compared to the mentioned literature. In response to this intervention was recorded an increased growth or base. The differences here did not manifest themselves as statistically significant during the first year after the intervention. Based on the results, which showed especially significant differences in the density of the cultivated stand with the non-interventional one can be recommended a very powerful intervention towards the release of the stand and the increase of stability.

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In response to the furniture industry’s growing demand for raw material, large volumes of juvenile silver birch and downy birch stems available from pre-commercial thinning operations in Sweden’s forests could offer solutions. However, such stems are not currently used on an industrial scale, and most research conducted on birch stems in general has neither focussed on young trees nor the potential use of the central stem part around the tree pith. The resulting lack of knowledge about the juvenile part of birch wood thus requires additional information about the material properties of birch, which could encourage its use for various purposes in the furniture and other industries. The initial literature review performed for this thesis has highlighted some properties of juvenile birch required for its use as a furniture material, as well as identified topics concerning the physical characteristics of juvenile birch about which knowledge is currently limited. Consequently, the objectives of this thesis were to explore some characteristics of juvenile birch—bark thickness, wood-to-bark bonding ratio after drying, variations in the density and width of growth rings, and anatomical growth response to fertilisation—in order to increase the knowledge. The material studied came from mixed birch and Norway spruce stands at two sites in southern Sweden, namely Asa and Toftaholm. The birch stems were naturally regenerated silver birch and downy birch, with breast height diameters between 30 and 83 mm. Fertilised and unfertilised silver birch trees were sampled at Toftaholm, whereas unfertilised stems of silver birch and downy birch were sampled at Asa. The characteristics of stems from the pith to bark (radial direction) and along the stem (longitudinal direction) were measured. The wood-to-bark bonding ratio on downy birch after drying was calculated as the percentage of the stem circumference with full contact between the wood and bark, while oven-dry density and basic wood density for silver birch were determined by using the water displacement method. The impact of ring width on wood density was statistically analysed, and an image analysis of the wood anatomy was conducted to elucidate their relationship. Amongst the results, bark thickness along the stem had the highest deviation in the section closest to the stump. Moreover, the wood-to-bark bonding ratio after drying measured for juvenile downy birch seemed to depend more on the stem’s diameter than the sampling height along the stem. Such results are relevant for processors seeking to estimate the volume of wood under the bark. The wood-to-bark bonding ratio was highest for diameters between 30 and 39 mm, and neither did that relationship correlate with the sampling height along the stem. Variation in wood density in the radial and longitudinal directions in juvenile silver birch suggested that such density negatively correlated with growth rate (ring width). That relationship held true for stems at each site and between the sites, irrespective of management or growing conditions. As expected, mean wood density was lower in fertilised trees than in unfertilised ones, and towards the bark, radial density increased more in trees that grew more slowly. At the same time, variation in longitudinal density in young silver birch trees was low. Quantitative wood anatomy studies confirmed that the fertilised juvenile birch had younger cambia, thinner cell walls, and fewer vessels per mm2 than unfertilised trees in the same diameter class. Overall, the knowledge generated in the study may facilitate the industrial use of juvenile birch stems and wood in interior and furniture applications. The role of wood anatomy in determining the mechanical performance of juvenile birch stems should be further examined, however, to possibly reveal new opportunities for the use of juvenile birch. / Det finns ett växande behov av råvaror till möbelindustrin. Stammar av juvenil vårtbjörk och glasbjörk finns tillgängliga i stora volymer från röjning i svenska skogar. För närvarande finns det inget storskaligt industriellt nyttjande av det virket. Bättre kunskap om dess virkesegenskaper kan öppna upp möjligheterna till olika användningsområden. Dock har en majoritet av den befintliga björkforskningen ofta exkluderat studier av innersta veden närmast kärnan, vilket resulterat i en låg kunskapsnivå om det juvenila virket. I den inledande litteraturstudien i denna uppsats belyses vilka krav som ställs på materialegenskaperna i möbelvirke. Vidare identifieras befintliga kunskapsluckor i fysiska och mekaniska egenskaper för juvenil björk. Målet med denna uppsats är därför att utforska några egenskaper, såsom barktjocklek, barkvidhäftning efter torkning, densitet och årsringsbreddsvariation samt hur gödsling kan påverka vedanatomin. Materialet kom från blandskogsbestånd med gran och björk i Asa och Toftaholm i Götaland. Björkarna var självföryngrad vårtbjörk och glasbjörk och stammarna hade en brösthöjdsdiameter mellan 30 och 83 mm. I Toftaholm var en del av beståndet gödslad respektive ogödslad vårtbjörk. Från Asa togs stammar av både vårtbjörk och glasbjörk. Virkesegenskaper och dess variationer i stammens radiella och longitudinella riktning studerades. Vidhäftningen av barken till veden efter torkning bedömdes procentuellt på omkretsen av ändträet. Relativ torrdensitet och rådensitet mättes. Påverkan av årsringsbredd på veddensitet analyserades statistiskt och vedanatomin studerades med mikroskop och bildanalys för en vidare förståelse av dess samband med gödsling. Resultaten gällande barktjocklekens variation visade att den nedre delen av stammen hade störst medelavvikelse. Dessa resultat kan vara relevant information för vidareförädling efter avbarkning. Barkens vidhäftning till veden efter torkning mättes på juvenil glasbjörk. Det visade ett visst samband med stamdiameter där den högsta vidhäftningen fanns på stammarna med en diameter mellan 30 och 39 mm. Sambandet var däremot inte korrelerat till stamhöjden där provet togs. Densitetsvariation i radiell och longitudinell riktning studerades för juvenil vårtbjörk. Densiteten hade en svag negativt korrelation till årsringsbredd. Detta samband sågs både inom bestånd och mellan bestånd med olika tillväxtförhållande samt skötsel. Medeldensiteten för virket var, som förväntat, lägre i de gödslade träden jämfört med de ogödslade. I den radiella riktningen  hade det senvuxna virket en högre faktor på densitetsökningen. Den longitudinella densitetsvariationen i unga vårtbjörkar var låg. Kvantitativa vedanatomistudier bekräftade att ungdomsveden i gödslad vårtbjörk hade en lägre kambiumålder, tunnare cellväggstjocklek och färre kärl jämfört med ogödslade träd med samma diameter, resultat som tidigare påvisats för mogen björk. Sammanfattningsvis kan resultaten från den här uppsatsen förbättra kunskapen och därmed underlätta för en industriell användning av juvenila björkstammar inom inredning och möbler. Dock bör vedanatomins påverkan på de mekaniska egenskaperna för juvenila björkstammar studeras vidare. Det kan möjliggöra nya användningsområden för juvenil björk.

<p>The great difference in the level of chloroplast variation and its geographic structure among the three main species studied here demonstrates that forest species do not form a homogeneous group. Hazel shows a genetic structure similar to many other thermophilous species and this structure, in combination with fossil evidence, indicates that the post-glacial colonisation of most of Europe originated in a refugium in western France while the Balkan and Italy were colonised from a south-eastern refugium.</p><p>In sallow and silver birch the chloroplast DNA variation and its structure does not fit with a scenario of glacial restriction to southern refugia and survival at intermediate latitudes is suggested for both species. The chloroplast DNA variation in silver birch suggests the presence of one western and one eastern European post-glacial colonisation route and limited contribution of southern populations in the colonisation of the rest of Europe. Unique haplotypes by the Ural Mountains indicates the possibility of a separate glacial origin of these populations.</p><p>The study of chloroplast DNA in species closely related to sallow and silver birch indicate that extensive hybridisation and cytoplasmic gene flow occurs within both the Salix and Betula genera in Europe. The nuclear and chloroplast phylogenies of 14 Betula species were not in complete agreement with each other or with the classical division of the Betula genus into subgenera or sections. The phylogenetic structure implies that hybridisation has played a role in the evolution of the Betula genus.</p><p>This thesis focuses on the chloroplast DNA variation in three forest tree genera: Corylus, Betula and Salix. Chloroplast PCR-RFLP is used to evaluate the post-glacial history of hazel, Corylus avellana, silver birch, Betula pendula and sallow, Salix caprea and to explore the possibility of introgression in the Salix and Betula genera. In addition, the chloroplast matK gene, its flanking regions and the nuclear ADH gene were used to study the phylogenetic relationships within the Betula genus.</p>