Made available in DSpace on 2014-12-17T14:13:34Z (GMT). No. of bitstreams: 1 PauloDPS_DISSERT_partes_autorizadas.pdf: 366490 bytes, checksum: 3e5abdd24789d0e82844edffadbb1a3e (MD5) Previous issue date: 2010-06-18 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The acidic galactan (AG) was obtained by extraction and proteolysis by acetone precipitation of the eggs of the mollusc Pomacea lineata. Its structure was elucidated by a combination of chemical analysis, the intrinsic viscosity and NMR spectroscopy 1D and 2D. Biological aspects of AG were evaluated by in vivo testing of healing and peritonitis induced (anti-inflammatory activity) and in vitro assays of cytotoxicity (MTT). This polymer showed a simple structure without the presence of sulfate and uronic acids in its structure. Its intrinsic viscosity and relative were evaluated at 0.44 ? 0.05 and 1.744? 0.07 dl.g-1. Spectroscopy showed that the AG has a constitution composed predominantly of β-D-galactosis, and β-D-glucosamine-NAcetil that comes in a smaller proportion in chain. The character of this acidic polysaccharide is given by the presence of pyruvate in the molecule, forming a cyclic acetal of six states, located in β-D-galactosis. The involvement of AG in the healing process was evaluated and the histological analysis revealed that there was so early in the process of healing, a great stimulation of macrophages with granuloma formation. Suggesting that AG may have promoted the advance of biological events required for tissue healing. In the trial of the GA-induced peritonitis showed dose dependent, demonstrating the anti-inflammatory effect at concentrations above 20 mg/kg, and confirming its inflammatory character and the concentration of 1mg/kg. In vitro tests used in the GA concentration of 1000 μg/mL showed proliferative activity by stimulating the growth of 3T3 cells, corroborating the findings in vivo and demonstrating the absence of cytotoxic activity / O Galactano ac?dico (GA) foi obtido atrav?s de extra??o por prote?lise e precipita??o com acetona das ovas do molusco Pomacea lineata. Sua estrutura foi elucidada atrav?s de uma combina??o de an?lises qu?micas, da viscosidade intr?nseca e de espectroscopia de Resson?ncia Magn?tica Nuclear, mono e bidimensionais. Os aspectos biol?gicos do GA foram avaliados atrav?s de ensaios in vivo de cicatriza??o e de peritonite induzida (atividade antiinflamat?ria); e ensaios in vitro da atividade citot?xica (MTT). Este polissacar?deo apresentou uma estrutura simples, sem presen?a de sulfato e ?cidos ur?nicos. Suas viscosidades intr?nseca e relativa foram avaliadas em 0,44 ? 0,05 e 1,744 ? 0,07 dL.g-1. A espectroscopia mostrou que o GA possui uma constitui??o formada predominantemente por β-D-galactoses, al?m de β- D-NAcetil-glucosamina que surge em menor propor??o na cadeia. O car?ter ac?dico deste polissacar?deo ? dado pela presen?a de piruvato na mol?cula, formando um acetal c?clico de 6-membros, localizados nas β-D-galactoses. A participa??o do GA na cicatriza??o foi avaliada e as an?lises histol?gicas revelaram que houve, de forma precoce ao processo de cicatriza??o, uma grande estimula??o de macr?fagos, com forma??o de granulomas. Sugerindo que o GA pode ter promovido a antecipa??o de eventos biol?gicos necess?rios ? cicatriza??o do tecido. No ensaio de peritonite induzida o GA se mostrou dose dependente, demonstrando uma a??o antiinflamat?ria em concentra??es acima de 20mg/kg, e comprovando seu car?ter inflamat?rio na concentra??o de 1mg/Kg. Em ensaios in vitro o GA utilizado na concentra??o de 1000 μg/mL apresentou atividade proliferativa estimulando o crescimento de c?lulas 3T3, corroborando os achados in vivo e demonstrando aus?ncia de atividade citot?xica