Previous studies have shown that rainbow trout exposed to dieldrin via diet for 9 to 12 weeks increased biliary excretion of a subsequent dose of [¹⁴C]dieldrin by 500%. This was not explained by induction of the cytochrome P-450 (CYP) system involved in oxidative metabolism of these compounds. We hypothesized that epoxide hydrolase activity increased in dieldrin fed-fish. Epoxide hydrolase is an enzyme that catalyzes the hydrolysis of epoxide compounds to their corresponding diols. For instance, dieldrin is metabolized to 6,7 trans-aldrindihydrodiol. This study investigated the activity of epoxide hydrolase in microsomes and cytosol of rainbow trout fed a diet that contained 0 or 15 ppm dieldrin. Fish were fed control or dieldrin diet (0.324 ug/g body weight/day) for 3, 6, or 9 weeks. There was a small increase in mortality and decrease in body weight among dieldrin-fed fish after 9 weeks. After week 9, dieldrin-fed fish were fed a control diet for an additional 3 weeks because of these signs of toxicity. At week 12, the difference of body weight between control and treated was not significant. Microsomal and cytosolic epoxide hydrolase activities were measured with a radiometric assay which determined differential partitioning of the parent compound (epoxide) in dodecane and the metabolite (diol) in the aqueous phase. Assays were run at optimal pH and temperature using [³H]trans-stilbene oxide (pH 7) as substrate for cytosol and [³H]cis-stilbene oxide (pH 8) as substrate for microsomes. In order to prevent competition for reaction with stilbene oxide, depletion of glutathione was efficiently achieved by dialysis at 4°C for 2 hours at room temperature in buffer [pH 7.5, potassium phosphate 10 mM, KCL 0.15 M, EDTA 1 mM, BHT 0.1 mM, 0.1 mM PMSF]. Protein quantification was determined by using BCA assay and concentrations were always between 5 and 25 ug/ml in the final assay volume. Epoxide hydrolase activities were not significantly different in cytosol or microsomes from control and dieldrin-fed fish. Dieldrin residues in liver were analyzed by gas chromatography with electron capture detection (GC/ECD). The concentration in the liver increased with time of exposure and declined markedly in fish fed dieldrin for 9 weeks and then fed control diet. No dieldrin was detected in livers from control fish. / Graduation date: 2003

Graduation date: 1994

Graduation date: 1995

Access to abstract permanently restricted to Ball State community only / Access to thesis permanently restricted to Ball State community only / School of Physical Education, Sport, and Exercise Science

Previous investigations have utilized extremes of diet and exercise during the week prior to a performance to raise muscle glycogen levels to 220 mmoles/kg wet tissue. This investigation was designed to determine if muscle glycogen levels can be supercompensated utilizing a combination of a depletion-taper sequence and diets of 15% carbohydrate (CHO, LC), 50% CHO (M), and 70% CHO (HC) in 3,000 kcal. Each depletion-taper sequence was conducted on a treadmill at 73% V02max and consisted of runs of 90, 40, 40, 20, 20 minutes and a day of rest on the six days prior to a 13 mile performance run. Trial C consisted of 3 days LC and 3 days HC; Trial B, consisted of 3 days M and 3 days HC; and Trial C, 6 days M. Muscle biopsies were obtained from the gastrocnemius prior to loading (day 4) and before and after the performance run. Trials A, B and C elevated muscle glycogen to 207, 203 and 160 mmoles/ kg wet tissue, respectively. Trials A and B resulted in significantly more glycogen utilization (105 and 107 mmoles/kg wet tissue, respectively) than did Trial C (65 mmoles/kg wet tissue). The times during the performance runs were not significantly different between the Trials. It is concluded that: 1. muscle glycogen levels can be supercompensated to levels comparable to previous investigations by employing a depletion-tapering sequence and less severe alteration in diet prior to competition; 2. carbohydrate loading is not necessary for trained runners preparing for a race of 13 miles.Supported by the National Dairy Council and the Ball State University Graduate Student Research Fund, 1980.Ball State UniversityMuncie, IN 47306

Serum lactage dehydrogenase and creatine phosphokinase level were studied in sixty male Sprague-Dawley rats weighing 400 to 680 grams. Five groups of ten rats each ran forty-five minutes (16 m/min) with variable percentages of the total bout consisting of downhill running (16° incline): Group I- 0%, Group II- 22%, Group III- 50%, Group IV- 78%, Group V- 100% downhill. Group VI consisted of ten sedentary controls. Serial blood samples were obtained immediately after exercise, 12, 24, 36, 48, and 60 hours found to exist between a non-trained rat’s weight and its ability to finish the exercise bout and live beyond 48 hours.Each exercise group, except Group V, showed s significant decrease in the CPK activity at 24 and/or 36 hours post-exercise. Group V showed a significant elevation (P<0.05) in LDH and CPK activity at 12 hours. With the total exercise time remaining the same, a linear relationship was suggested between duration of downhill running and LDH activity at 12 and 24 hours post-exercise, r=0.5880 (n=26) and r=0.4490 (n=28), respectively.Ball State UniversityMuncie, IN 47306

The purpose of this investigation was to quantify the acute pressor response to caffeine at rest and during exercise, and to compare responses for different reportings of caffeine consumption and sensitivity. Eight young men with high use (HU) consumption patterns (<600 mg/day) and eight with low use (LU) patterns (<90 mg/day) participated. Caffeine (C) (4.5 mg/kg fat free mass) and placebo (P) were administered, double-blind and counterbalanced. Systolic (SBP), diastolic blood pressure (DBP) and heart rate (HR) were monitored during baseline, 40 minutes of absorption and graded treadmill walking. Oxygen consumption (V02) and rating of perceived exertion (RPE) were monitored during exercise. No significant differences were found for the responses to C between HU and LU nor between sensitive and not sensitive groups. Following a 12 hour abstinence, caffeine significantly increase SBP and DBP at absorption compared to P (11/9 mm Hg). The increase was additive with exercise for SBP but not DBP. Caffeine significantly increased VO2 compared to P. No significant differences were found for HR nor RPE between C and P trials. The present data indicate chronic caffeine consumption does not build tolerance to the acute pressor response to caffeine ingestion, the resting pressor response is additive with the pressor response to exercise for SBP, and individual perceptions of caffeine sensitivity are not accurate predictors of pressor responses. / School of Physical Education

The purpose of this study was to analyze specific biomechanical and physiological variables in disabled swimmers performing a functional backcrawl. The effect of selectively placed flotation devices on the body position, exercise heart rate, and perceived exertion of the swimmer was compared to swimming with no flotation. The participants in the study included eight able-bodied males 21.5 (+2.51) yrs. and four disabled males 29.25 (+2.22) yrs. Each subject performed the backcrawl for 3 consecutive lengths of a 25 meter pool under four separate trial conditions: free swimming, swimming with the hip flotation device, the knee flotation device, and the ankle flotation device. Immediately following each trial a post-exercise heart rate was taken and the subject was asked to rate perceived exertion. Full recovery was allowed after each trial. Trials were video taped and digitized to determine the mean angles at the neck, trunk, hip, and knee during one complete stroke cycle. The investigator recognizes that this generalization presents the possibility for a bias in the data and that further study would warrant the utilization of the different phases of the stroke for determination of body angles. The results of the study demonstrated that a significant difference did exist between the disabled and able-bodied hip angle during free swimming. Within the disabled group, no significant differences were evident when comparing the body angles during free swimming to the same angles while swimming with each of the flotation devices. Correlations between heart rate and ratings of perceived exertion for all subjects (n=12) were significant in each of the four trial conditions. / School of Physical Education

published_or_final_version / Nursing Studies / Master / Master of Nursing

abstract / Social Work and Social Administration / Doctoral / Doctor of Philosophy