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Lateral rotation of the left and right hip, knee, ankle and intertarsal joints during three trials of turnout from the straight leg and demi-plie positions was quantified with the use of a specially designed friction-free weightbearing goniometer, projecting fin-like body markers, and overhead photography. The female subjects were dancers (10 beginners, 11 advanced) recruited from University of Arizona ballet classes. MANOVAS revealed that turnout as measured from the feet was (1) significantly greater in the advanced group in both positions, (2) significantly greater in the demi-plie position than in the straight leg, (3) a cumulative joint rotation effect with the hip contributing the greatest absolute and relative amounts. Positive significant correlations occurred between: (1) pedal turnout and lateral hip rotation for the advanced group in both positions and for the beginning group in demi-plie, and (2) pedal turnout and lateral ankle rotation for the advanced dancers in the straight leg position. Alignment of the lower extremity segments during turnout from both positions did not exist for either group.

The effect of ethanol on the stress response in rats was examined. Experimental animals were given 0.25 ml of 28 percent ethanol or 0.25 ml of water orally once a day, five days a week, for a period of twelve months and were then subjected to fifteen minute cold stress. Corticosterone levels in ethanol-treated males following stress were significantly lower (22 percent) than in the sham group. Adrenal weights in sham-treated females were significantly higher (15 percent) than in the ethanol group at the end of twelve months. Mortality in sham-treated males was significantly higher (60 percent) than in ethanol-treated males. The effects observed may be due to the sedative action of ethanol on cortical centers controlling the hypothalmus.

The potential for human exposure to pesticides exists particularly for agricultural workers (i.e. migrant workers) and individuals within close proximity to pesticide-sprayed crops (i.e. those living on or near agricultural farms). Children, through biology and behavior, may be more susceptible and vulnerable to exposure to pesticides than adults. The purpose of this study was to examine young populations particularly at-risk for occupational or accidental exposure to pesticides and determine associated behavioral, emotional, and physical symptoms. A total of 444 students from two South Texas school districts completed questionnaires assessing level of risk of exposure to pesticides and were categorized into at-risk and low risk categories. Physical, emotional, and behavioral symptoms were obtained using the Youth Self-Report. Children who were at-risk demonstrated significantly higher scores on the Youth Self-Report (YSR) in the areas of anxious/depressed, attention problems, social problems, somatic complaints, thought problems, withdrawal, internalizing behaviors, and total problem behaviors than children who were at lower risk of pesticide exposure. Odds ratios were obtained and suggested that children in the at-risk category were more five times more likely to score in the clinically significant range on the Attention Problems subscale, and three times more likely to score in the clinically significant range on the Internalizing behavior composite. These findings suggest that children who may be at higher risk for pesticide exposure may also be at higher risk for physical, behavioral, and emotional problems compared to children who are at lower risk. This information is intended to benefit schools and health care professionals who work with rural or migrant populations involved in the agricultural trade. Future research will be needed to assess through biomarkers the degree of measurable pesticide exposure in comparison to parent reports, teacher reports, school achievement, neuropsychological testing, and medical records.

Call number: LD2668 .T4 1952 C6 / Master of Science

Call number: LD2668 .T4 1957 O95 / Master of Science

Ph.D., Faculty of Science, University of the Witwatersrand, 2011 / The ABCB1 gene encodes P-glycoprotein, a transmembrane protein that regulates the efflux of drugs in the cells and may affect the response to antiretroviral drugs. ABCB1 polymorphisms affect the function or expression of P-gp. The 3435T allele has been associated with decreased protein production, but is in linkage disequilibrium with other polymorphisms. HIV is prevalent in Southern Africa, and characterization of ABCB1 variation may provide insight into its role in antiretroviral immune response. The aim was to determine if there was any association between ABCB1 variation, relative mRNA levels and immune response. Seven known polymorphisms were characterized for linkage disequilibrium and haplotype analysis, regions upstream of the gene were sequenced for bioinformatic analysis, the relative amounts of mRNA were determined, and CD4+ and viral load data was analyzed for association. Sequencing revealed six novel variations: T-137G, C-233T and G-298A upstream of exon 1, T108G and G153A in exon 2, and A111G in intron 26. The frequencies of the -129T (0.85), 1236T (0.70), 2677G (0.77), IVS 25+3050G (0.86), IVS 25+5231T (0.51), 3435C (0.88) and IVS 26+80T (0.89) polymorphisms were different and LD was lower compared to other populations. The haplotype frequencies were different to other populations and the genetic structure was probably a result of multiple recombination or mutation events. The viral load counts at the second measurement after baseline (time point 2) were significantly different from baseline for the 2677GG and 2677GA genotypes, and the -129T allele was associated with a lower proportional decrease in viral load at 8 the second measurement. The IVS 25+3050GG, 3435CC and IVS 26+80TT genotypes have been associated with lower mean relative mRNA levels. In conclusion, the genetic structure of the southern African populations is different from other populations and that genetic association and functional studies derived from other populations would be irrelevant in this population. A larger sample size and functional studies would be required to attempt to resolve the molecular mechanisms of the ABCB1 gene and to confirm the findings of association between ABCB1 polymorphisms and immune response.