Thesis (Ph.D.)--Tufts University, 2002. / Adviser: Colin M. Orians. Submitted to the Dept. of Biology. Includes bibliographical references (leaves 111-127). Access restricted to members of the Tufts University community. Also available via the World Wide Web;

Thesis (Ph. D.)--Washington State University, December 2006. / Includes bibliographical references.

Apesar de sua elevada importância econômica mundial, o cultivo do tomateiro é classificado como de alto risco devido à infestação da cultura por um grande número de pragas e doenças. Esse problema leva tal cultura a depender amplamente da aplicação de agroquímicos que, além de elevaram o custo de produção, são potenciais causadores de danos ambientais e do aparecimento de outras pragas e doenças. Observando o problema mais detalhadamente, pode-se perceber que uma grande parcela das perdas advém dos danos causados por artrópodes herbívoros, os quais são capazes de se alimentar de diversas partes da planta, causando, geralmente, perda de produtividade. Apesar disso, o tomateiro apresenta mecanismos de defesa naturais contra tais pragas, como tricomas e aleloquímicos, que agem intoxicando os artrópodes, dificultando sua movimentação e/ou alterando varias fases de seu desenvolvimento. A busca por genótipos de tomateiro com maior densidade de tricomas e elevado teor de aleloquímicos é hoje considerado um hot spot de pesquisa, devido ao benefício que trariam como redução do custo de produção e maior produtividade. Sabese que vários hormônios vegetais estão ligados a geração de caracteres anti-herbivoria, mas, atualmente, um grande foco vem sendo dado somente ao ácido jasmônico, devido ao seu claro papel na formação de tricomas, aleloquímicos, inibidores de proteases, dentre outros. Visando mostrar a importância de uma abordagem multi-hormonal nesse tipo de estudo, o presente trabalho teve como objetivo avaliar quais hormônios vegetais estão envolvidos com a formação de vários caracteres anti-herbivoria em tomateiro, como densidade de tricomas e teor de aleloquímicos e inibidores de protease. Para tal, fez-se uso da um grande número de mutantes hormonais introgredidos na cultivar Micro-Tom (a qual apresenta diversas facilidades de trabalho como porte reduzido e rápido ciclo de vida). Demonstrou-se que, apesar do ácido jasmônico ser extremamente importante na formação de tais caracteres, outros hormônios também atuam fortemente em tal papel. Etileno, giberelinas e auxina alteram de forma indireta a densidade de tricomas em tomateiro, através de alteração na área de células epidérmicas. O ácido jasmônico é um forte regulador positivo da formação de tricomas, do aleloquímico zingibereno e da formação de inibidores de protease. De forma antagônica, brassinosteróides parecem controlar negativamente a densidade de tricomas, a produção de zingibereno e inibidores de protease. Interessantemente, observou-se que esse controle negativo efetuado por brassinosteróides acontece através de um controle na via do ácido jasmônico, o qual foi comprovado pela produção de duplos mutantes. Resultados obtidos em testes com o herbívoro polífago Spodoptera frugiperda e com a praga de tomate Tuta absoluta (traça-do-tomateiro), bem como de análise de expressão gênica comprovaram a importância do ácido jasmônico, brassinosteróides e sua interação na defesa a herbivoria. Os resultados aqui apresentados sugerem que o foco para futuros estudos da formação de mecanismos anti-herbivoria em tomateiro deve ser voltado não só para o ácido jasmônico como para brassinosteroides e para suas ações antagônicas. / Even though the cultivation of tomato has a high economical importance, this culture is classified as being of elevated risk because of it common infestation by pests and diseases. This problem makes this culture greatly dependent of the application of several agrochemicals, which leads to increase in cost of production, environmental damages and also facilitating the appearance of new pests and diseases. Looking carefully to the problem, it is possible to conclude that a great part of tomato losses are caused by herbivory-arthropods, which are capable of feeding of several parts of the plant, causing, generally, losses in the productivity. However, tomato has natural defense mechanisms against those pests, for example trichomes and allelochemicals, which act poisoning the arthropods, hindering their movement through the plant and/or altering steps of their development. The search for tomato genotypes with great density of trichomes and elevated levels of allelochemicals is nowadays being considered as a hot spot of research, because of the benefits it would provide (for example: a decrease in the cost of production and also a higher productivity). Although it is believed that many plant hormones are involved in the generation of anti-herbivory traits, a great focus has been given only to jasmonic acid because of it clear action in the formation of those traits, such as trichomes, allelochemicals, proteinase inhibitors and many others. The present work objective was to evaluate which hormones are involved in the formation of anti-herbivory traits such as trichome density and allelochemicals and proteinase inhibitors content. For this reason, we made use of several hormonal mutants already introgressed in the Micro-Tom cultivar (which presents benefits as small size and fast life cycle). It was showed that jasmonic acid is an important hormone in the formation of these traits, but other phytohormones also play important roles. Ethylene, gibberellins and auxin alters trichome density indirectly, by altering the area of epidermal cells and thus cell number. Jasmonic acid is a positive regulator of trichome formation, the allelochemicals zingiberene content and also in the formation of proteinase inhibitors. However, brassinosteroids acts by negatively controlling all of those. Interestingly, we observed that this negative control happens through the control of jasmonic acid pathway, a hormonal interaction that was proved by double-mutant analysis. Results obtained in tests with the polyphagous insect Spodoptera frugiperda and the tomato specific pest Tuta absoluta (tomato pinworm) and also with qPCR showed the importance of jasmonic acid, brassinosteroid and their interaction in herbivory defense. Our data suggest that future studies about the formation of antiherbivory traits should be analyzed by focusing the functions not only of jasmonic acid but also brassinosteroid and their antagonist functions.

Apesar de sua elevada importância econômica mundial, o cultivo do tomateiro é classificado como de alto risco devido à infestação da cultura por um grande número de pragas e doenças. Esse problema leva tal cultura a depender amplamente da aplicação de agroquímicos que, além de elevaram o custo de produção, são potenciais causadores de danos ambientais e do aparecimento de outras pragas e doenças. Observando o problema mais detalhadamente, pode-se perceber que uma grande parcela das perdas advém dos danos causados por artrópodes herbívoros, os quais são capazes de se alimentar de diversas partes da planta, causando, geralmente, perda de produtividade. Apesar disso, o tomateiro apresenta mecanismos de defesa naturais contra tais pragas, como tricomas e aleloquímicos, que agem intoxicando os artrópodes, dificultando sua movimentação e/ou alterando varias fases de seu desenvolvimento. A busca por genótipos de tomateiro com maior densidade de tricomas e elevado teor de aleloquímicos é hoje considerado um hot spot de pesquisa, devido ao benefício que trariam como redução do custo de produção e maior produtividade. Sabese que vários hormônios vegetais estão ligados a geração de caracteres anti-herbivoria, mas, atualmente, um grande foco vem sendo dado somente ao ácido jasmônico, devido ao seu claro papel na formação de tricomas, aleloquímicos, inibidores de proteases, dentre outros. Visando mostrar a importância de uma abordagem multi-hormonal nesse tipo de estudo, o presente trabalho teve como objetivo avaliar quais hormônios vegetais estão envolvidos com a formação de vários caracteres anti-herbivoria em tomateiro, como densidade de tricomas e teor de aleloquímicos e inibidores de protease. Para tal, fez-se uso da um grande número de mutantes hormonais introgredidos na cultivar Micro-Tom (a qual apresenta diversas facilidades de trabalho como porte reduzido e rápido ciclo de vida). Demonstrou-se que, apesar do ácido jasmônico ser extremamente importante na formação de tais caracteres, outros hormônios também atuam fortemente em tal papel. Etileno, giberelinas e auxina alteram de forma indireta a densidade de tricomas em tomateiro, através de alteração na área de células epidérmicas. O ácido jasmônico é um forte regulador positivo da formação de tricomas, do aleloquímico zingibereno e da formação de inibidores de protease. De forma antagônica, brassinosteróides parecem controlar negativamente a densidade de tricomas, a produção de zingibereno e inibidores de protease. Interessantemente, observou-se que esse controle negativo efetuado por brassinosteróides acontece através de um controle na via do ácido jasmônico, o qual foi comprovado pela produção de duplos mutantes. Resultados obtidos em testes com o herbívoro polífago Spodoptera frugiperda e com a praga de tomate Tuta absoluta (traça-do-tomateiro), bem como de análise de expressão gênica comprovaram a importância do ácido jasmônico, brassinosteróides e sua interação na defesa a herbivoria. Os resultados aqui apresentados sugerem que o foco para futuros estudos da formação de mecanismos anti-herbivoria em tomateiro deve ser voltado não só para o ácido jasmônico como para brassinosteroides e para suas ações antagônicas. / Even though the cultivation of tomato has a high economical importance, this culture is classified as being of elevated risk because of it common infestation by pests and diseases. This problem makes this culture greatly dependent of the application of several agrochemicals, which leads to increase in cost of production, environmental damages and also facilitating the appearance of new pests and diseases. Looking carefully to the problem, it is possible to conclude that a great part of tomato losses are caused by herbivory-arthropods, which are capable of feeding of several parts of the plant, causing, generally, losses in the productivity. However, tomato has natural defense mechanisms against those pests, for example trichomes and allelochemicals, which act poisoning the arthropods, hindering their movement through the plant and/or altering steps of their development. The search for tomato genotypes with great density of trichomes and elevated levels of allelochemicals is nowadays being considered as a hot spot of research, because of the benefits it would provide (for example: a decrease in the cost of production and also a higher productivity). Although it is believed that many plant hormones are involved in the generation of anti-herbivory traits, a great focus has been given only to jasmonic acid because of it clear action in the formation of those traits, such as trichomes, allelochemicals, proteinase inhibitors and many others. The present work objective was to evaluate which hormones are involved in the formation of anti-herbivory traits such as trichome density and allelochemicals and proteinase inhibitors content. For this reason, we made use of several hormonal mutants already introgressed in the Micro-Tom cultivar (which presents benefits as small size and fast life cycle). It was showed that jasmonic acid is an important hormone in the formation of these traits, but other phytohormones also play important roles. Ethylene, gibberellins and auxin alters trichome density indirectly, by altering the area of epidermal cells and thus cell number. Jasmonic acid is a positive regulator of trichome formation, the allelochemicals zingiberene content and also in the formation of proteinase inhibitors. However, brassinosteroids acts by negatively controlling all of those. Interestingly, we observed that this negative control happens through the control of jasmonic acid pathway, a hormonal interaction that was proved by double-mutant analysis. Results obtained in tests with the polyphagous insect Spodoptera frugiperda and the tomato specific pest Tuta absoluta (tomato pinworm) and also with qPCR showed the importance of jasmonic acid, brassinosteroid and their interaction in herbivory defense. Our data suggest that future studies about the formation of antiherbivory traits should be analyzed by focusing the functions not only of jasmonic acid but also brassinosteroid and their antagonist functions.

Thesis (doctoral)--Swedish University of Agricultural Sciences, 2003. / Thesis documentation sheet inserted. Includes appendix of four papers and manuscripts, two co-authored with Barbara Ekbom. Includes bibliographical references. Also partially issued electronically via World Wide Web in PDF format; online version lacks appendix of papers.

Aggregation and species association of 9 species of phytophagous and predaceous mites were studied for 6 yr in an orchard with 100, 2 m tall 'Red Delicious' apple trees. To quantify aggregation, a method was developed that is powerful, and easily interpreted. It relates the proportion of habitat occupied to mean density of the organism, and allows discrimination of aggregation differences between data sets. We found that plant feeders were more aggregated than predators, and active life stages were less aggregated than eggs. Specifically, webspinning spider mites (Tetranychus urticae + Eotetranychus sp.) were the most aggregated. Panonychus ulmi was less aggregated than the webspinning mites, and Bryobia rubrioculus was the least aggregated of the spider mites. Zetzellia mali, the slowest moving predator, had the greatest aggregation of all the predators. The rapid moving phytoseiids were the least aggregated of all the mite species studied, with the specialist predator Metaseiulus occidentalis having the greatest aggregation, the generalist predator Typhlodromus pyri having medium aggregation, and the fast moving Amblyseius andersoni having the least aggregation. Predator-predator, predator-prey, and prey-prey associations were measured using Yule's V association index. Predator-predator associations were the strongest and most consistent, showing a consistent seasonal pattern of neutral-negativeneutral association. Negative associations of T. pyri with other predators were the strongest, which is consistent with evidence that this mite can detect other predators. Predator-prey seasonal associations were weak and mixed, and interactions between prey species were generally weakly positive, probably because of similar habitat preferences. Predaceous mites were generally more aggregated when competing with other predators, possibly allowing the coexistence of 3 predators simultaneously for 6 years via mechanisms proposed by the "aggregation theory of coexistence". G. occidentalis showed the greatest change of aggregation when other predators were present, Z. mali and T. pyri also showed significant changes in aggregation when they were with other predators, but A. andersoni (the largest, fastest predator in our study) showed no changes in aggregation. T. pyri's aggregation increased the most when in the presence of Z. mali, perhaps because of egg predation by the stigmaeid, or because T. pyri could detect the other predator. / Graduation date: 1999

Thesis (M.S.)--University of Delaware, 2006. / Principal faculty advisor: Douglas W. Tallamy, Dept. of Entomology & Wildlife Ecology. Includes bibliographical references.

Thesis (M.S.)--Ohio State University, 2009. / Title from first page of PDF file. Includes vita. Includes bibliographical references (p. xv-130).

Thesis (Ph. D.)--University of Adelaide, Dept. of Crop Protection, 1996. / Addendum in pocket. Biblography: leaves 162-173.

Solanum mauritianum Scop. (bugweed), a serious exotic weed in South Africa, supports a depauperate herbivore fauna relative to indigenous Solanum species. These comprise mainly polyphagous, and some oligophagous, species. The greater diversity of insect herbivores on Solanum hermannii Dun., relative to other indigenous Solanum species in the eastern Cape, suggests that the plant is indigenous and not exotic as has been suggested. Five oligophagous species seasonally caused a high incidence of damage to S.hermannii and other indigenous Solanum species in the eastern Cape, during the study period. Galls of the flowers, stems and leaves of some indigenous Solanum plants are described and biological data on the gall-formers and their natural enemies presented. The tortoise beetle, (Chrysomelidae), defoliates a Conchyloctenia tigrina wide range of indigenous Olivo Solanum species. The exotic S.mauritianum and S.elaeagnifolium Cav. were unfavourable for growth and survival. S.hermannii proved the most favourable of the indigenous hosts tested, for growth and survival, providing further evidence that it is indigenous. Extensive studies on different field populations of the beetle revealed no evidence of host adaptation (host races). All populations tested displayed greater fitness when reared on S.hermannii. South African Solanum faunas are characterized by a scarcity of endophagous and monophagous herbivores, vacant feeding niches and low numbers of species relative to other plant taxa. This may suggest evolutionary immaturity of the insect-plant associations, although comparative studies of solanums from other parts of the world are needed for confirmation. Alternatively, these characteristics may simply be a feature of the genus Solanum in general. The depauperate herbivore fauna of S.mauritianum reflects a lack of local herbivores suitably preadapted to overcome its defences. This appears to be accentuated by absence of any close relatives of bugweed in South Africa. The greater diversity of herbivores in its native South America suggests that biological control may be a potentially useful means of control for bugweed. The potential for biological control is, however, threatened by the susceptability of the cultivated eggplant (Solanum melongena L.) to attack by Solanum-feeding insects. Possible solutions to this problem are discussed