The aim of this investigation in Offatts Bayou was to quantify natural shifts in distributions and abundances of algal groups and to examine spatio-temporal patterns of abiotic and biotic characteristics in the water column over a one year period. To accomplish this, hydrological and meteorological parameters were collected and phytoplankton biomass, community composition and chlorophyll a data were examined for significant relationships. Seasonal variations in water temperature, salinity, dissolved oxygen concentrations and pH levels, as well as wind effects, zooplankton grazing, light availability and hydrodynamic restriction were considered as the key controlling factors in phytoplankton dynamics in Offatts Bayou. Surface water samples and water column hydrological data were collected at eleven stations in the Offatts Bayou embayment on a regular basis (2 to 4 times per month), along with phytoplankton tows on a monthly basis. Spatial patterns of phytoplankton abundance generally reflected the degree of circulation in Offatts Bayou with higher abundances observed in the restricted areas and lower abundances in the well mixed regions. Temporally, diatom blooms became more prominent during winter, spring and autumn, which were characterized by cooler temperatures, less light availability, increased dissolved oxygen concentrations and reduced salinities than observed in summer. The most dominant diatoms were Guinardia delicatula, Ditylum brightwelli, Rhizosolenia setigera, Dactyliosolen fragillissimus and numerous Chaetoceros species. During summer, the waters of Offatts Bayou were warmer and more saline, which lead to the haptophyte, Corymbellus aureus, becoming the dominant taxa, with highest standing crops at the circulation restricted stations in Lake Madeline. While the results of this study support the importance of temperature, dissolved oxygen and pH as the critical controlling factors (p < 0.05) of phytoplankton biomass and diversity, it is clear that phytoplankton dynamics in Offatts Bayou must be viewed within the broader context of additional parameters such as salinity, stratification and wind effects. The progressively degrading conditions within Offatts Bayou emphasize the significance of studying and understanding the interrelationships of factors and mechanisms that influence phytoplankton dynamics. Long term monitoring of Offatts Bayou is essential for tracking, recording and assessing various human impacts to phytoplankton distribution, abundance, and productivity as well as impacts to higher trophic levels such as fish and humans.

Phytoplankton mirrors changes in the environment and plays an important role in biogeochemical processes. Phytoplankton dynamics is the outcome of both autogenic succession and external disturbances. This thesis focused on the seasonal variation of water column stability and its effects on phytoplankton, particularly considering the influence of mixing events on phytoplankton development. Lake Erken is a dimictic lake with weak and often interrupted summer stratification, which represents an intermediate case between a polymictic lake and a lake with strong summer stratification. There are two diatom phases annually. The spring bloom is caused by pioneer centric diatoms, and the autumn diatom phase is dominated by meroplanktonic diatoms induced by turnover. A summer Cyanobacteria bloom – mainly Gloeotrichia echinulata, depended on the length and stability of stratification. Winter and spring air temperature is found to play an important role in the annual succession of phytoplankton by initiating changes in ice/snow-cover and lake thermal stability and setting the basic status. Instead of starting from zero, the vernal phytoplankton piles up on the overwintering community, this trans-annual ecological memory influences both the composition and diversity and taxonomic distinctness of spring phytoplankton. Water column stability during summer in Lake Erken is mainly influenced by wind-induced turbulence and internal seiches. As thermal stratification develops from early until late summer, variations in stability and gradual deepening of the thermocline depth influence phytoplankton dynamics directly by changing its distribution, and also indirectly by altering the nutrient and light availability. A new disturbance index (DI) was defined to quantify environmental stability/disturbance and tested well to indicate phytoplankton equilibrium status in two summer stratification periods. The concept of species and functional groups was generally used in this study. However, a next generation sequencing based approach was also tested and proved to provide an excellent candidate for revealing distribution patterns of phytoplankton in inland waters.

Mechanisms controlling the productivity, abundançe and taxonomic composition of phytoplankton in tropical Lake Malawi were examined by monitoring phytoplankton dynamics in 8 regions covering the length of the lake (560 km) over a 10-12 month period, and relating these dynamics to spatio-temporal changes in thermal structure, nutrient availability, and meteorological conditions. In addition, nearshore benthic photosynthetic rates were measured in 7 different months.Spatial and temporal changes in areal photosynthetic rates were due almost entirely to changes in the efficiency of light utilization by the phyroplankton community. An evaluation of potential factors which might influence tight utilization indicates that nutrient availabitity is the most important. Most of the photosynthetic N and P demand is met by internal recycling within the upper 200 m, and therefore spatio-temporal variation of phytoplankton photosynthetic rate is closely related to changes in mixing regime. It is shown that the dominant meteorological factors responsible for changes in the mixing regime were solar radiation and windspeed. A comparison with previous photosynthesis data for Lake Malawi indicates that windspeed is a dominant factor controlling interannual variability. Shallow areas of the lake were more productive than deep areas, due to more intense upwelling and more efficient internal nutrient recycling in shallow waters. Within the littoral zone, benthic photosynthetic rates were very high, accounting for 14% to 28% of total net photosynthesis within the shallow southeast arm. Phytoplankton biomass was not correlated with photosynthetic rate, indicating that biomass loss processes were important in controlling biomass variability. Changes in phytoplankton taxonomic composition were related to changes in mixing regime. Cyanobacteria and chlorophytes were dominant throughout much of the study period, but diatoms made up a significant proportion of total biomass during periods of increased turbulence and nutrient availability. An analysis of phytoplankton surface area : volume ratios revealed that organism shape and size are important determinants in species succession. Previous studies have emphasized the low variability of phytoplankton biomass and photosynthetic rates in tropical lakes, relative to temperate lakes. An inter-lake comparison reveals that this tenet does not apply to large lakes. Fluctuations in the mixing regime of large tropical lakes have an effect on phytoplankton variability similar in magnitude to the effect of fluctuating solar irradiance in large temperate lakes.

Mechanisms controlling the productivity, abundançe and taxonomic composition of phytoplankton in tropical Lake Malawi were examined by monitoring phytoplankton dynamics in 8 regions covering the length of the lake (560 km) over a 10-12 month period, and relating these dynamics to spatio-temporal changes in thermal structure, nutrient availability, and meteorological conditions. In addition, nearshore benthic photosynthetic rates were measured in 7 different months.Spatial and temporal changes in areal photosynthetic rates were due almost entirely to changes in the efficiency of light utilization by the phyroplankton community. An evaluation of potential factors which might influence tight utilization indicates that nutrient availabitity is the most important. Most of the photosynthetic N and P demand is met by internal recycling within the upper 200 m, and therefore spatio-temporal variation of phytoplankton photosynthetic rate is closely related to changes in mixing regime. It is shown that the dominant meteorological factors responsible for changes in the mixing regime were solar radiation and windspeed. A comparison with previous photosynthesis data for Lake Malawi indicates that windspeed is a dominant factor controlling interannual variability. Shallow areas of the lake were more productive than deep areas, due to more intense upwelling and more efficient internal nutrient recycling in shallow waters. Within the littoral zone, benthic photosynthetic rates were very high, accounting for 14% to 28% of total net photosynthesis within the shallow southeast arm. Phytoplankton biomass was not correlated with photosynthetic rate, indicating that biomass loss processes were important in controlling biomass variability. Changes in phytoplankton taxonomic composition were related to changes in mixing regime. Cyanobacteria and chlorophytes were dominant throughout much of the study period, but diatoms made up a significant proportion of total biomass during periods of increased turbulence and nutrient availability. An analysis of phytoplankton surface area : volume ratios revealed that organism shape and size are important determinants in species succession. Previous studies have emphasized the low variability of phytoplankton biomass and photosynthetic rates in tropical lakes, relative to temperate lakes. An inter-lake comparison reveals that this tenet does not apply to large lakes. Fluctuations in the mixing regime of large tropical lakes have an effect on phytoplankton variability similar in magnitude to the effect of fluctuating solar irradiance in large temperate lakes.

Thesis (MSc)--Stellenbosch University, 2011. / ENGLISH ABSTRACT: An integro-differential equation describing the dynamical behaviour of phytoplankton cells is considered in which the effects of cell division and aggregation are incorporated by coupling the coagulation-fragmentation equation with growth, and the McKendrick-von Foerster renewal model of an age-structured population. Under appropriate conditions on the model parameters, the associated initial-boundary value problem is shown to be well posed in a physically relevant Banach space using the theory of strongly continuous semigroups of operators, the theory of perturbation of positive semigroups and the semilinear abstract Cauchy problems theory. In particular, we provide sufficient conditions for honesty of the model. Finally, the results on the effects of the growth-fragmentation-coagulation on the overall evolution of the phytoplankton population are summarised. / AFRIKAANSE OPSOMMING: ’n Integro-differensiaalvergelyking wat die dinamiese ontwikkeling van fitoplanktonselle beskryf, word beskou. Die uitwerking van seldeling en -aggregasie is geïnkorporeer deur die vergelyking van koagulasie en fragmentasie met groeiaan die McKendrick-von Foerster hernuwingsmodel van ’n ouderdomsgestruktureerde populasie te koppel. Die teorie van sterk kontinue semigroepe van operatore, steuringsteorie van positiewe semigroepe en die teorie van semilineêre abstrakte Cauchy probleme word aangewend om, onder gepaste voorwaardes met betrekking tot die model se parameters, te bewys dat die geassosieerde beginwaarde-probleem met randvoorwaardes ‘goed gestel’ is in ’n fisies relevante Banach-ruimte. In die besonder word voldoende voorwaardes vir eerlikheid van die model verskaf. Ten slotte word ’n opsomming van die resultate met betrekking tot die gekombineerde uitwerking van groei-fragmentasie- koagulasie op die gesamentlike ontwikkeling van die fitoplanktonpopulasie verskaf.

Biological invasion has become a major threat to economy, ecology, global biodiversity and ecosystem function of aquatic ecosystems. The main aim of the thesis was to study the effects of the zebra mussel (Dreissena polymorpha), a versatile invasive species, on phytoplankton dynamics and ecosystem function of lakes. In a first attempt, I compared the density of Dreissena and the physicochemical data of ecosystems that it invaded among North American and European lakes to identify important factors in its invasion success. Secondly, I investigated the impact of zebra mussels on phytoplankton community composition in a natural lake. Thirdly, I evaluated whether zebra mussel feeding behavior were affected by the presence of predatory waterborne cues. Finally, I examined the effect of Dreissena on seston stoichiometry. A Generalized Additive Model revealed that a joint effect of surface area, mean depth, total phosphorus and calcium concentrations can explain the variability in Dreissena density. Selective grazing by zebra mussels varied in relation to seasonal phytoplankton dynamics. Risk cues released by predators affected both feeding rate and prey selection of the mussels and had cascading indirect effects on phytoplankton biomass and community structure. I found that the flux in nutrients caused by differences in zebra mussel consumption lead to a variation in phytoplankton nutrient limitation. The flexibility of zebra mussel feeding behavior and variation in susceptibility among phytoplankton groups to mussel ingestion indicate that invading zebra mussels could alter phytoplankton community composition of lakes and have important ecosystem consequences. The results of this thesis contribute to the growing evidence that predators indirectly affect resource dynamics and food web structure through their non-lethal effects on consumers. The results suggested that zebra mussel can indirectly both reduce and increase the energy transfer efficiency from primary producers to upper trophic levels in the pelagic and benthic food webs, respectively.

<p>Biological invasion has become a major threat to economy, ecology, global biodiversity and ecosystem function of aquatic ecosystems. The main aim of the thesis was to study the effects of the zebra mussel <i>(Dreissena polymorpha)</i>, a versatile invasive species, on phytoplankton dynamics and ecosystem function of lakes. </p><p>In a first attempt, I compared the density of <i>Dreissena</i> and the physicochemical data of ecosystems that it invaded among North American and European lakes to identify important factors in its invasion success. Secondly, I investigated the impact of zebra mussels on phytoplankton community composition in a natural lake. Thirdly, I evaluated whether zebra mussel feeding behavior were affected by the presence of predatory waterborne cues. Finally, I examined the effect of <i>Dreissena</i> on seston stoichiometry.</p><p>A Generalized Additive Model revealed that a joint effect of surface area, mean depth, total phosphorus and calcium concentrations can explain the variability in <i>Dreissena</i> density. Selective grazing by zebra mussels varied in relation to seasonal phytoplankton dynamics. Risk cues released by predators affected both feeding rate and prey selection of the mussels and had cascading indirect effects on phytoplankton biomass and community structure. I found that the flux in nutrients caused by differences in zebra mussel consumption lead to a variation in phytoplankton nutrient limitation.</p><p>The flexibility of zebra mussel feeding behavior and variation in susceptibility among phytoplankton groups to mussel ingestion indicate that invading zebra mussels could alter phytoplankton community composition of lakes and have important ecosystem consequences. The results of this thesis contribute to the growing evidence that predators indirectly affect resource dynamics and food web structure through their non-lethal effects on consumers. The results suggested that zebra mussel can indirectly both reduce and increase the energy transfer efficiency from primary producers to upper trophic levels in the pelagic and benthic food webs, respectively. </p>