京都大学 / 新制・課程博士 / 博士(理学) / 甲第23041号 / 理博第4718号 / 新制||理||1676(附属図書館) / 京都大学大学院理学研究科生物科学専攻 / (主査)教授 中野 伸一, 教授 曽田 貞滋, 教授 木庭 啓介 / 学位規則第4条第1項該当 / Doctor of Science / Kyoto University / DGAM

Few genomic and metagenomic studies have focused on freshwater systems in the last years. Most of the studies carried out on these particular environments so far rely on microscopy, physiology, phenotypic observations, individual genes and 16S rRNA sequencing. Here, we shed light on microbial communities from oligotrophic and mesotrophic freshwater systems using high-throughput deep sequencing metagenomics and genome-resolved metagenomics. We have focused on the study of ubiquitous and cosmopolitan microbial groups from two temperate Spanish reservoirs (Tous, Amadorio). Among these, we studied freshwater picocyanobacteria from Synechococcus and Cyanobium genera, which so far have not been well characterized at the genomic level, compared to the marine representatives. In particular, we were able to isolate two of the most abundant picocyanobacteria from Tous reservoir, which were previously studied via metagenomics. These picocyanobacteria are not only abundant in this reservoir but are widely distributed in different freshwater and brackish systems. In this work we also shed light on some of the first freshwater representatives of the phylum Verrucomicrobia, that are ecologically uncharacterized in freshwater systems about which relatively little is known. We discovered a wide range of metabolisms in these microbes, ranging from nitrogen fixation and photoheterotrophy via rhodopsin pumps to important contributions in the degradation of recalcitrant matter and polysaccharides. We also include the first metagenomic study of the microbial communities under the ice waters of the largest (by volume) ultraoligotrophic lake in the world, Lake Baikal. This study has provided a first glimpse and a particular microbial composition on the sub-ice, having found an unusual fraction of Verrucomicrobia and new microbial lineages from many typical freshwater phyla, including the first freshwater representative of the groups I/II of SAR11 lineage and novel genomes of Proteobacteria, Thaumarchaeaota, Gemmatimonadetes, Cyanobacteria, Planctomycetes, Bacteroidetes, Acidobacteria, Nitrospirae, Verrucomicrobia or Actinobacteria.

No description available.

Blooms of nitrogen fixing cyanobacteria (NFC) occur in many freshwater and marine systems, including the Baltic Sea. By fixing dissolved nitrogen, they circumvent general summer nitrogen limitation, while also generating a supply of novel bioavailable nitrogen for non-diazotrophic primary producers and ultimately supporting secondary production. Elucidating trophic links between primary consumers and NFC is essential for understanding role of these blooms for secondary production. However, until recently, there was no reliable method to quantify individual prey species for zooplankter feeding in situ. The development of PCR-based methods to detect prey-specific DNA in the diet of consumers, including microscopic animals, allows identification and quantification of trophic linkages in the field. Using molecular diet analysis in combination with egg production measurements, biochemical markers of growth and condition; and stable isotope approach, we explored a possibility to determine (1) whether cyanobacteria are grazed and assimilated by mesozooplankters (Papers I and II), (2) which species/groups are particularly efficient consumers of cyanobacteria (Papers II and III), and (3) how feeding on cyanobacteria affects zooplankton growth and development (Paper I and III). Taken together, these laboratory and field observations, provided evidence that NFC contribute to feeding and reproduction of zooplankton during summer and create a favorable growth environment for the copepod nauplii (Paper I). The favorable growth conditions for juvenile copepods observed during NFC blooms were hypothesized to be mediated by picoplankton that take up bioavailable nitrogen exuded from cyanobacterial cells. This hypothesis found support in Paper II that provided quantitative estimates for the direct picocyanobacteria → mesozooplankton pathway, with highest weight-specific consumption observed in nauplii. Further, using field observations on zooplankton and phytoplankton development during a growth season in the northern Baltic proper, we found that NFC nitrogen is assimilated and transferred to zooplankton via both direct grazing and indirectly through grazing on small-sized phyto- and bacterioplankton (Paper III). Finally, these and other findings emphasizing the importance of NFC for Baltic Sea secondary production during growth season were synthesized to show that diazotrophic nitrogen enters food webs already at bloom initiation (Paper III) and is transferred via multiple pathways to pelagic and benthic food webs and, ultimately, to fish (Paper IV). / <p>At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 3: Manuscript. Paper 4: Accepted.</p>

No description available.

No description available.