In order to identify expressologs (orthologs exhibiting the highest expression profile ranking) among a variety of plant species, bioinformatic methods were used in order to first identify sequence orthologs and subsequently to rank these orthologs based on expression profile similarity. Analyses conducted on these data suggested that expressologs exhibited greater functional equivalency. A comparison of drought response in A. thaliana and Populus showed that expressologs exhibited a higher correlation when computed using stress data as opposed to developmental data. This suggested that the use of condition-specific data sets is more appropriate when examining specific conditions. Analysis was conducted in order to investigate the hypothesis that neutral evolution was a predominant factor in gene expression divergence. Some evidence was found for selection acting on expression pattern maintenance. Further analysis will be required in order to confirm the type of selection acting to maintain expression patterns across species.

Tree transplanting practices influence plant survival, establishment, and subsequent landscape value. The inability to adequately quantify effects of inappropriate tree planting and transplanting practices threatens long-term viability and productivity (sustainability) of trees within terrestrial ecosystems. Tree planting depth, i.e. location of the root collar relative to soil grade, is of particular concern for tree growth, development, and performance in the landscape. A series of model studies was conducted to investigate effects of planting depth, container production methods, and transplanting practices on landscape establishment of container-grown trees. Studies included determining the effect of planting depth and soil amendments on live oak (Quercus virginiana Mill.) and baldcypress (Taxodium distichum (L.) L. Rich.), the effect of planting depth during container production and subsequent landscape establishment of lacebark elm (Ulmus parvifolia Jacq.), the effect of planting depth and irrigation practices on landscape establishment of sycamore (Platanus occidentalis L.), and the effect of planting depth and transplant season on landscape establishment of baldcypress. Optimum planting depth varied among species and was dependent on cultural practices and/or environmental conditions. Overall, live oak and baldcypress growth was better when planted with root collars at grade in sand in raised beds compared to planting below grade in control soils. Lacebark elm growth was greater when planted at grade during the initial container production phase and below grade in the second container production phase. Subsequent landscape establishment was variable, but planting at grade to 5 cm above grade produced greater growth. Sycamore trees planted below grade had increased mortality and decreased growth compared to trees planted at grade or above grade, while irrigation had no effect. Baldcypress planted above grade had reduced growth compared to those planted at or below grade, while transplant season had no effect. Species and cultivars within species may differ markedly in their response to environmental/cultural stresses, including planting depth. Each tree species originating from a specific environment may represent an ecotype adapted to that particular environment. Therefore, tree survival and performance may depend on the difference between the environment from which the tree was grown and the experimental system into which it is introduced.

Invasive alien plants have impacted southern U.S. forests. This study focuses on quantifying current distribution, rate of dispersals, and economic losses caused by four major invaders — Chinese tallow (Triadica sebifera (L.) Small), Chinese Privet (Ligustrum sinense Lour.), European Privet (Ligustrum vulgare L.), and Japanese Honeysuckle (Lonicera japonica Thunb.). Using data from USDA Forest Service’s Forest Inventory and Analysis (FIA), National Oceanic and Atmospheric Administration (NOAA), and USGS Land Use and Land Cover (LULC), I first used logistic regressions to build occupation models for these four species. Second, I used VB.NET© (Microsoft, 2003) and Environment System Research Institute (ESRI) Arc View® 9.2 Geographical Information System (GIS) to model spatially explicit dispersal dynamics of these species. Finally, I evaluated economic losses associated with these invasions. The results of occupation models showed that landscape features, climatic conditions, forest and site conditions, forest management activities and disturbances, and forest ownership have statistically significant impacts on current distributions of all four species. The results of dispersal models showed that invasions of Chinese tallow, Chinese and European privets, and Japanese honeysuckle will be likely to appear on approximately 6.65 million acres of forest land in East Texas and Louisiana, 3.81 million acres in Mississippi and Alabama, and 12.55 million acres in Mississippi and Alabama, respectively, during the next 20 years. The estimated timber losses resulting from the invasion with no control for the next 20 years at 5% discount rate is 518 million dollars for Chinese tallow, 2.72 billion dollars for Chinese and European privets, and 11.05 billion dollars for Japanese honeysuckle. From an economic point of view, it would be most cost effective to execute high intensity control for these invasive species. Based on these findings, strategies for monitoring and mitigating these invasive species are proposed.

Recently, the use of constructed wetlands to treat nutritions and containing wastewater, has quite been in vogue. However, since salts aquacultural wastewater contains massive nutrients and salts, the common aquatic plant species are not suitable to treat it. Therefore the purpose of this research is mainly to use pilot-scale salty water constructed wetland systems operated under batch conditions to removal efficiency of nutrients. The pilot-scale have nine pools, the influent was from discharge channels containing aquacultural wastewater and community sewage near by Dapeng Bay. The experiment designs altogether has four stages.In the first stage to the third stage, the HRT was continued at 7 days. Last stage, the HRT was continued at one month. Experimental period has used waste brick bat, oyster shell, 9mm stone and mix stone (9mm stone and 3mm stone mix) as filter medium. The 9mm stone pool and mix stone pool coordinates differently to test the stage planter differently to plant the species. The plant aspect has Avicennia marina(L2¡BL3 pool ), Lumnitzera racemosa and Phragmites australis. (First, the second stage plants the Phragmites australis , the third stage starts to plant Lumnitzera racemosa). The experimental results showed that the filter media used with the mix stone presented the highest removal efficiency for the nutrients,whice the oyster shell exhibited the lowest removal efficiency for the nutrients. The oyster shell system was also found nutrients releaseal to the system. For using plants to remove the nutrients, we found that plants, did not show better performace than the systems without plants(p¡Ö0.05). However, for the condition of vegetation, the plant species of Avicennia marina exhibited the best performace.

In the pursuit of sustainable development, Environmental Impact Assessments (EIA) are acknowledged globally as a tool designed to assist governing authorities by providing the information required to make an informed decision regarding development proposals. South Africa has entrenched this EIA requirement in the presiding environmental legislation: the National Environmental Management Act (Act 107 of 1 998). In the effort to manage the negative impact of development on the rocky ridges of Johannesburg, the Gauteng Department of Agriculture, conservation, Environment and Land Affairs (GDACEL) has introduced a buffer zone requirement in the procedure of the EIA. The Red Data Plant Policy for Environmental Impact Evaluations for GDACEL described a buffer zone as a collar of land that filters out inappropriate influences from surrounding activities. As a tool in the EIA, a buffer zone is a worthwhile concept. However, the determination of the dimension of the buffer zone on rocky ridges, is non-discriminatory between sites, and thus, presents potential contention between decision-making authorities and developers. There is a need for further research to establish a scientifically acceptable method of determining site-specific buffer zones for individual EIA applications. The key objective of this paper is to suggest the possibility of determining a buffer zone that accommodates the unique environmental aspects of each site. This is achieved by determining the distance between the edge of existing developments and the point at which the successional climax community within the adjacent natural vegetation is established. Three suitable study sites, consisting of developed residential estates on ridges adjacent to nature reserves, were identified within the greater Johannesburg metropolis. The three study sites identified for this assessment include Kloofendal (west), Morning Hill (east) and Kliprivier (south). Within each study site field surveys were conducted along transects starting 5m from the development edge and ending 75m within the nature reserve adjacent to each site. Quantitative (species density) and qualitative (Braun-Blanquet cover-abundance values) data analysis was employed to describe and evaluate the identified plant communities. The data in this study provides clear indication that a 25-35m buffer zone would suffice for these specific plant communities to maintain a climax successional status if impacted on by residential development. This paper thus makes a case for permitting the determining of variable buffers zones, based on a gradient analysis of a plant community, as a potential panacea to the problem of resistance and reluctance to accept present standard buffer zones. / Thesis (M. Environmental Management)--North-West University, Potchefstroom Campus, 2007.

No description available.

<p>We hypothesize that South African medicinal plants contain compounds that can act in synergism with synthetic antifungal compounds. Four fungicides - Sporekill&trade / , Rovral&trade / , Terminator&trade / and Teldor&trade / at doses 0.1, 0.2, 0.4 and 0.8 mL L-1 and plant species Galenia africana, Elytropappus rhinocerotis and Tulbaghia violacea were tested aloneand in different combinations for their potency (efficacy) on radial growth inhibition of Botrytis cinerea strains on potato dextrose plates. Four doses of plant extract for each of the respective plant species were used. A total of 48 combinations were tested for each strain. Mixtures of plant extracts were far more effective in controlling strains compared to the individual components alone, representing significant levels of in vitro synergistic interactions. Combinations of these components represent an attractive future prospect for the development of new management strategies for controlling B. cinerea. Since the in vitro tests of these mixtures showed inhibitory activity, the mixtures were tested for activity in assays on Granny Smith apples. In vitro tests can be used to screen mixtures to obtain information on their inhibitory activity on a pathogen, however, the environmental conditions of the fruit and the ability of the pathogen to grow into the fruit cannot be simulated in vivo. A series of two-fold doses of medicinal plant extracts were combined with fungicides to conduct decay inhibition studies. The incidence of gray mold was significantly reduced by mixtures of plant extracts and fungicides. Under conditions similar to those in commercial storage, a drench treatment with G. africana and Rovral&trade / significantly (p=0.05) inhibit gray mold on the apples and was more effective than the plant extract and fungicide alone. The treatments exerted synergistic effects and were markedly better than the components applied alone. The wound colonization assay was used for optimal decay control. In a drench, much higher volumes of the treatments are used to ensure that the components of the suspension are deposited evenly over the entire fruit surface. Drenching of fruit to apply other chemicals is an established practise in the pome (fleshy) fruit industry, and simplifies the commercial application of the mixtures, as no additional infrastructure at commercial packing houses will be required. This approach not only makes it possible to reduce fungicide concentrations while maintaining adequate decay control, but also ensures a reduction of the chemical residue on the fruit.</p>

Flowering is one of the most important stages in determining the successful survival and spread in plants. The duration of the flowering period is closely associated with successful reproduction, making it essential to understand the importance and effects of the length of flowering on various macroecological variables across plant species. The effects of the length of flowering periods on the distribution range size of species have seldom been investigated. This project aims to identify any macroecological relationship that may exist between the length of flowering periods and the distribution ranges of plant species endemic to the eastern part of South Africa, a region well known for its floral diversity. Range size and flowering phenology data were collected for several genera that are centred in the region (Cussonia, Gymnosporia, Searsia, Streptocarpus, Pavetta, Plectranthus, Crinum, Eulophia, Gladiolus, Kniphofia, Satyrium, Watsonia and Zantedeschia). At genus level, the relationship varied considerably. While significant correlations between the two variables were retrieved in four genera, the meaning of these patterns differed. In some cases, these suggested that a larger range was achieved through successful pollination due to extended flowering periods, whereas in others, it is probably just an effect of different flowering seasons in different areas where the range is large enough to comprise diverse climates. When incorporating variables such as growth form (narrowly and broadly-defined) and genus identity in analyses of covariance between flowering durations and various measures of distribution, the association of genera was far greater than that of growth form. It can be concluded that both range size and the length of the flowering season are the result of numerous factors acting jointly, which differ across plant groups and are likely to be susceptible to changes in climate and biological invasions. This means that the relationship between range size and flowering period is driven by different factors in different genera, suggesting that the conservation of plant diversity in the face of global change will have to consider the complexity of flowering patterns, and it is likely that lineage-specific approaches for different plant groups will be necessary. / Thesis (M.Sc.)-University of KwaZulu-Natal, Westville, 2012.

South Africa is a megadiverse country, and its biodiversity is endangered by population pressure and the development needs of a developing country. In order to address the rapid decline in biological diversity, biodiversity planning has become a key focus area that aims at identifying priority areas for species and ecosystem conservation within and outside of formally protected areas. Plant conservation hotspots are identified by the quantification of indicator taxa such as plant taxa richness, rarity and endemism. But the urgent and enormous task of biodiversity assessment for conservation planning requires that we make most of what we know. Therefore, this study seeks to make a contribution by finding new ways of biodiversity pattern estimation from the extrapolation of incomplete sets of plant species distribution data at the Quarter Degree Grid level. Incomplete sampling across the grids of a study area results in false records of species absence and thus a biased biodiversity estimation. As a possible solution, plant distribution data for the western Central Bushveld Bioregion has been standardized using two profiles, namely the ‘Centroid Grid’ and ‘Integrated Grid’ profile. The former involves the strengthening of under-sampled grids by extrapolating species occurrences from three adjacent grids with the most similar vegetation units, whereas the latter integrates phyto-diversity data for the four grids intersecting at each grid reference point. Standardized data has proved to provide a means to counter the bias in plant diversity data linked to Quarter Degree Grids by a) strengthening of under-sampled grids and b) visibly smoothing out the gaps between under- and well-sampled grids, which resulted in improved biodiversity estimation for more representative spatial biodiversity modelling. Interpolation created geo-referenced polygons for more explicit use in the identification of areas of conservation concern at bioregional scale. However, well-sampled grids still dominate the outcomes of the analysis by creating spatial sampling bias. Therefore, this approach to calibrate Quarter Degree Grid resolution of spatial data is an additional attempt to achieve more representative mapping of biodiversity patterns, which is a prerequisite for strategic conservation planning for ‘living landscapes’. / Thesis (MSc (Environmental Sciences))--North-West University, Potchefstroom Campus, 2012

South Africa is a megadiverse country, and its biodiversity is endangered by population pressure and the development needs of a developing country. In order to address the rapid decline in biological diversity, biodiversity planning has become a key focus area that aims at identifying priority areas for species and ecosystem conservation within and outside of formally protected areas. Plant conservation hotspots are identified by the quantification of indicator taxa such as plant taxa richness, rarity and endemism. But the urgent and enormous task of biodiversity assessment for conservation planning requires that we make most of what we know. Therefore, this study seeks to make a contribution by finding new ways of biodiversity pattern estimation from the extrapolation of incomplete sets of plant species distribution data at the Quarter Degree Grid level. Incomplete sampling across the grids of a study area results in false records of species absence and thus a biased biodiversity estimation. As a possible solution, plant distribution data for the western Central Bushveld Bioregion has been standardized using two profiles, namely the ‘Centroid Grid’ and ‘Integrated Grid’ profile. The former involves the strengthening of under-sampled grids by extrapolating species occurrences from three adjacent grids with the most similar vegetation units, whereas the latter integrates phyto-diversity data for the four grids intersecting at each grid reference point. Standardized data has proved to provide a means to counter the bias in plant diversity data linked to Quarter Degree Grids by a) strengthening of under-sampled grids and b) visibly smoothing out the gaps between under- and well-sampled grids, which resulted in improved biodiversity estimation for more representative spatial biodiversity modelling. Interpolation created geo-referenced polygons for more explicit use in the identification of areas of conservation concern at bioregional scale. However, well-sampled grids still dominate the outcomes of the analysis by creating spatial sampling bias. Therefore, this approach to calibrate Quarter Degree Grid resolution of spatial data is an additional attempt to achieve more representative mapping of biodiversity patterns, which is a prerequisite for strategic conservation planning for ‘living landscapes’. / Thesis (MSc (Environmental Sciences))--North-West University, Potchefstroom Campus, 2012