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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
91

Development of chromatographic bioseparations based on lectins and supermacroporous affinity cryogels

Raletjena, Moloko Ivonne January 2012 (has links)
Thesis (M.Sc. (Biochemistry)) -- University of Limpopo, 2012 / Various cytomorphologic and biochemical markers of apoptosis are found in different compartments (plasma membrane, cytoplasm, nucleus, and mitochondria) of target cells. Although the plasma membrane is an easily accessible cellular compartment, relatively little is known about the changes in the expression of plasma membrane glycoproteins during apoptosis, and whether these changes could be used for detection of apoptosis. A critical element of this study was to purify lectins from crude homogenate on glycoprotein-cryogel affinity matrices, and later use the lectins to detect changes on the cell surface of apoptotic cells. Pterocarpus angolensis seed lectin was extracted and fractionated using ammonium sulphate precipitation. The 60 % ammonium sulphate pellet was dissolved in saline azide and purified using Sephadex G-75 affinity chromatography. A 28 kDa lectin was retarded within the column and appeared as a short and broad peak on the chromatogram. Traditionally, Sephadex G-75 column are used predominantly for size exclusion, in this study, the column was used in a non-traditional way for affinity chromatography, as the purified protein is able to bind sugar moieties existing in the structure of Sephadex G-75. A single-step purification of P. angolensis seed lectin was achieved by directly applying unclarified P. angolensis crude extract to the pAAm-cryogel using fetuin as the affinity ligand. Pterocarpus angolensis extract fractionated into 2 peaks, which revealed a highly concentrated band on SDS-PAGE. The results also revealed that an increased binding of the lectin to the fetuin-cryogel matrices was also dependent on the time of incubation. This study suggested very low capacities of the cryogels for the protein due to low coupling sites on the matrix. Taking into account that lectins serve as invaluable tools in diverse area of biomedical research, this study proposed using specific plant lectins to follow the expression of plasma membrane glycoproteins during programmed cell death. Treatment of HL-60 cells with lithium and actinomycin D confirmed a time- and dose-dependent inhibition of proliferation and a decrease in proliferation, which suggest cell death of the treated cells. The observed cell death was further investigated for cellular and biochemical hallmark features of apoptosis, which has shown preferential binding of annexin V-FITC to phosphatidylserine and low molecular DNA ladder. Several FITC labelled lectins were used to detect changes in cell surface glycosylation that accompany apoptosis. This study xvii has shown amongst several FITC-labelled lectins that T. vulgaris lectin could intensively stain the membrane area of apoptotic cells suggesting that the expression of N-acetylglucosamine was significantly increased during actinomycin D induced apoptosis of HL-60 cells. Binding was shown to be specific because it was blocked by the corresponding inhibitory sugar. Thus, the method described in this study could be suitable for the detection of very early stages of apoptosis by recognizing the cell surface carbohydrates of apoptosis.
92

The structure and genetic control of endosperm proteins in wheat and rye

Singh, Nagendra Kumar. January 1985 (has links) (PDF)
Bibliography: leaves [129]-146
93

Computational studies of protein stabilization and denaturation by small molecules /

Bennion, Brian James. January 2003 (has links)
Thesis (Ph. D.)--University of Washington, 2003. / Vita. Includes bibliographical references (leaves 183-205).
94

The structure and genetic control of endosperm proteins in wheat and rye / by Nagendra Kumar Singh

Singh, Nagendra Kumar January 1985 (has links)
Bibliography: leaves [129]-146 / v, 146, [50] leaves, [50] leaves of plates : ill ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Agronomy, Waite Agricultural Research Institute, 1985
95

The structure and genetic control of endosperm proteins in wheat and rye / by Nagendra Kumar Singh

Singh, Nagendra Kumar January 1985 (has links)
Bibliography: leaves [129]-146 / v, 146, [50] leaves, [50] leaves of plates : ill ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Agronomy, Waite Agricultural Research Institute, 1985
96

Replacement of poultry by-product meal in production diets for the Pacific white shrimp (Litopenaeus vannamei)

Markey, Justin C. Davis, D. Allen January 2007 (has links) (PDF)
Thesis(M.S.)--Auburn University, 2007. / Abstract. Vita. Includes bibliographic references.
97

Identification and characterisation of Vitis vinifera pathogenesis-related proteins that accumulate during berry ripening /

Tattersall, David Bruce. January 1999 (has links) (PDF)
Thesis (Ph. D.)--University of Adelaide, Dept. of Horticulture, Viticulture and Oenology, 1999. / Includes bibliographical references (leaves 138-158).
98

Dependence of substrate-water binding on protein and inorganic cofactors of photosystem II /

Hendry, Garth S. January 2002 (has links)
Thesis (Ph.D.)--Australian National University, 2002.
99

Cell wall proteins and growth maintenance of the maize primary root at low water potentials /

Wu, Yajun, January 1996 (has links)
Thesis (Ph. D.)--University of Missouri-Columbia, 1996. / Typescript. Vita. Includes bibliographical references. Also available on the Internet.
100

Cell wall proteins and growth maintenance of the maize primary root at low water potentials

Wu, Yajun, January 1996 (has links)
Thesis (Ph. D.)--University of Missouri-Columbia, 1996. / Typescript. Vita. Includes bibliographical references. Also available on the Internet.

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