Presented dissertation thesis is focused on the possibility of using the crumb rubber from waste tires for modification of paving grade bitumen. The resulting asphalt rubber can be used as an alternative to polymer modified bitumen in compacted bituminous mixtures. The theoretical part describes the rheological properties of asphalt binders and the history and today state of the art in the world and in the Czech Republic. The practical part of the work contains a description of the properties of chosen crumb rubbers and produced asphalt rubbers focused on their rheological properties and on change of these properties caused by the aging at high temperature. Subsequently, the chosen empirical and functional properties of bituminous mixtures with asphalt rubber are described. Laboratory properties of these mixtures were verified under real service conditions on 32 test sections. Based on the dissertation thesis results the preliminary technical specification of the Ministry of Transport (TP 148) and their following revision were approved. The conclusion of the work summarizes and highlights the results of the dissertation.