Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-09-18T13:59:23Z No. of bitstreams: 1 2017 - Verona Borges Ferreira.pdf: 1252759 bytes, checksum: 291a4f24b8d135e118f8e80afed31cfd (MD5) / Made available in DSpace on 2017-09-18T13:59:23Z (GMT). No. of bitstreams: 1 2017 - Verona Borges Ferreira.pdf: 1252759 bytes, checksum: 291a4f24b8d135e118f8e80afed31cfd (MD5) Previous issue date: 2017-02-21 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Brazil is among the world?s largest fish producers, and the state of Rio de Janeiro is one of the main responsible for this production. In addition, there is a substantial increase in fish consumption which is associated to its functional and nutritional properties, such as its lipid profile. Today the hydrographic bays of Rio de Janeiro suffer the impacts of excessive use of toxic substances, such as Organoclorine Pesticides (OCPs), which has a characteristic lipophilicity and tend to accumulate in adipose tissue of organisms. As a consequence, this may pose risk to consumers of fish from polluted environments. The human exposure to these pollutants is highly related to health problems, such as cancer, immunity diseases and endocrine disruptions. Thus, the present study aims at investigating the occurence of OCPs in sardine (Sardinella brasiliensis), whitemouth croaker (Micropogonias furnieri) and mullet (Mugil liza) from Guanabara Bay, located in the state of Rio de Janeiro, and also to estimate the OCPs intake through fish consumption. The analyses were carried out at the Laboratory of de Radioisotopes Eduardo Penna Franca at UFRJ. The analitical steps included sohxlet extration, purification and subsequent injection in a Gas Chromatography system coupled to a Mass Spectrometer on Negative Chemical Ionization mode (GC/MS/ENCI) to determine the OCPs concentrations. The estimative of the toxic compound intake was performed based on the obtained data for OCP in fish samples, the fish intake of the brazilian population, andavailable data of Acceptable Daily Intake (ADI) for each investigated pesticide. All samples had at least one OCP. Metoxichlor had the highest concentration for the three studied species. There was significant difference between the species concerning the concentration of o,p?-DDD and o,p?-DDT. These DDT metabolites were found at higher concentration in sardine samples in comparison to croaker and mullet ones. However, there was no significant difference between the species in relation to ?OCP. It was evidenced the greater proportion of DDE among the DDT metabolites which suggests the past use of this pesticide. Results for ?-HCH isomer indicate the contamination by the use of Lindane instead of the technical HCH mixture. There was no significant difference among the contamination of the three studied species in regard to sample's acquisition locations, showing that the locality does not influence the content of OCPs in fish, probably due the species behavior which travel big distances along the brazilian coast throughout their life cycle. No sample exceeded the maximum residue limit stipulated for OCPs at both national or international level. Based on the estimated intake data no species exceeded the toxicological safety parameter. Thus, the three studied fish species can be considered safe for human consumptiom in regard to OCPs exposure / O Brasil est? entre os maiores produtores mundiais de pescado, sendo o estado do Rio de Janeiro um dos principais respons?veis por essa produ??o. Al?m disso, existe um aumento substancial do consumo de peixes, que est? associado ? suas propriedades nutricionais e funcionais, como o seu excelente perfil lip?dico. As bacias hidrogr?ficas do Rio de Janeiro sofrem hoje os impactos do uso excessivo de subst?ncias t?xicas, como os Pesticidas Organoclorados (OCPs), que devido ? caracter?stica lipof?lica, tendem a se acumular no tecido adiposo de organismos aqu?ticos, o que pode representar um risco a sa?de de quem consome pescado oriundos de ambientes contaminados. Por sua vez, a exposi??o humana a estes poluentes est? altamente relacionada a problemas de sa?de, tais como o surgimento de c?ncer, problemas de imunidade e interfer?ncias end?crinas. Desta forma, o presente estudo tem por objetivo investigar a ocorr?ncia de OCPs em amostras de sardinha-verdadeira (Sardinella brasiliensis), corvina (Micropogonias furnieri) e tainha (Mugil liza) capturados na Ba?a de Guanabara, localizada no estado do Rio de Janeiro, assim como estimar a ingest?o de OCPs por meio do consumo de pescado. As an?lises foram realizadas no Laborat?rio de Radiois?topos Eduardo Penna Franca da UFRJ, atrav?s de sua extra??o, purifica??o e posterior inje??o em um sistema de Cromatografia Gasosa acoplada a um Espectr?metro de Massa por ioniza??o qu?mica negativa (CG/EM/NCI) para determinar as concentra??es de OCPs. Para estimar a ingest?o desses compostos t?xicos foram utilizados os dados de contamina??o das amostras, consumo do pescado pela popula??o brasileira, assim como os dados de Ingest?o Di?ria Aceit?vel (IDA) para cada contaminante. Todas as amostras investigadas estavam contaminadas com pelo menos um OCP, o Metoxicloro foi o pesticida encontrado em maior concentra??o nas tr?s esp?cies estudadas e houve diferen?a significativa entre as esp?cies em rela??o a concentra??o de o,p?-DDD e o,p?-DDT, estes metab?litos do DDT foram encontrados em maior concentra??o nas amostras de sardinha-verdadeira em rela??o as amostras de corvina e tainha, entretanto n?o houve diferen?a significativa entre as esp?cies em rela??o ao ?OCP. Ficou evidenciada a maior propor??o do DDE entre os metab?litos de DDT, n?o sugerindo o uso recente deste pesticida, e do is?mero ?-HCH entre os demais is?meros de HCH, evidenciando a contamina??o pelo uso de Lindano em rela??o ao HCH t?cnico. N?o houve diferen?a significativa entre a contamina??o das tr?s esp?cies com base nos diferentes pontos de coleta, demonstrando que a localidade de aquisi??o do pescado n?o influenciou no teor dessas subst?ncias, uma vez que estas esp?cies ao longo de seu ciclo de vida percorrem grandes dist?ncias pela costa brasileira. Nenhuma amostra excedeu os limites m?ximos de res?duo estipulados para esses contaminantes tanto a n?vel de regulamenta??o nacional quanto internacional. Com base nos dados de estimativa de ingest?o nenhuma esp?cie excedeu o par?metro toxicol?gico de seguran?a, sendo assim, as tr?s esp?cies estudadas s?o consideradas seguras para o consumo humano em rela??o a exposi??o a OCPs