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Application of quantitative polymerase chain reaction in the diagnosisof thalassaemiaTsang, Tsui-ying, Stella., 曾璀瑩. January 2006 (has links)
published_or_final_version / Medical Sciences / Master / Master of Medical Sciences
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Performance evaluation of the automated NucliSens easyMAG and Qiagen EZ1 Advanced XL nucleic acid extraction platform for detection of RNAand DNA viruses in clinical samplesLam, Yiu-pong., 林耀邦. January 2011 (has links)
published_or_final_version / Microbiology / Master / Master of Medical Sciences
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Studies of the role of the iron oxidizing bacterium Thiobacillus ferrooxidans in marine and terrestrial environments : development of a novel detection assayGronow, Kathryn L. January 2000 (has links)
No description available.
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Vectors for high fidelity mycoplasma genitalium DNA cloning and expression /Dubowsky, Andrew. Unknown Date (has links)
Thesis (PhD(HealthSciences))--University of South Australia, 2001.
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Isolation and Characterization of Pseudobutyrivibrio ruminis Gene Promoterstdschoep@yahoo.com, Tobias Delavilla Schoep January 2004 (has links)
A family of E. coli - P. ruminis shuttle-plasmids was constructed to allow the isolation
and characterization of gene promoters from the rumen bacterium P. ruminis. The
promoter rescue plasmid pBK was used to isolate a total of 4 genomic DNA fragments
that promoted transcription in P. ruminis strains 0/10. These promoters, and an
additional promoter, previously isolated from P. ruminis strain OR38 (Schoep, 1999),
were identified by their ability to initiate expression of a promoterless ermAM gene in
P. ruminis. Within 4 of the fragments, a total of 5 transcription start sites were identified
in P. ruminis using a novel, fluorescent-primer extension analysis protocol. Comparison
of promoters isolated in this and previous studies revealed a strong consensus RNA
polymerase DNA-binding motif, including the well characterized 35 and 10 elements.
Consensus sequences established for these elements were: TTgacA and AtAATAta
respectively, where bold upper-case font, regular upper-case, and lower-case fonts
represent conservation in 100%, 80%, and 70% of promoters respectively. The −10 and
−35 motifs were interspaced by 16 18 nt. Among the newly identified promoters, the
consensus for the 10 element was extended one nucleotide upstream and downstream
of the standard hexamer (boxed). These motifs were similar to those recognized by
eubacterial RNA polymerase containing the σ70-like factor. Promoters also contained
possible UP elements, and were significantly more curved than protein-coding regions.
Additional plasmid vectors were constructed, to allow the use of both the quantitative
SYBR green real time PCR and ß-glucuronidase assays, to examine 4 promoters in
depth. This showed a wide range of promoter strengths within the group. However, no
correlation was found between the composition and context of elements within P.
ruminis promoters, and promoter strength. A mutation within the 35 element of one
promoter revealed that promoter strength, and the choice of transcription start site were
both sensitive to single nucleotide
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Geschlechtsdiagnose bei Vögeln mit Hilfe der Polymerase-Kettenreaktion (PCR)Hoffmann, Brigitte January 2006 (has links)
Univ., Diss., 2005--Giessen
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Purification of general RNA polymerase II transcription factors from mouse for studies of proliferation-specific transcription /Kotova, Irina, January 2003 (has links)
Diss. (sammanfattning) Umeå : Univ., 2003. / Härtill 3 uppsatser.
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The development, implementation and evaluation of a real-time PCR-based diagnostic service for viral causes of infectious intestinal diseaseGunson, Rory Niall. January 2007 (has links)
Thesis (Ph.D.) - University of Glasgow, 2007. / Ph.D. thesis submitted to the Division of Community Based Sciences, Faculty of Medicine, University of Glasgow, 2007. Includes bibliographical references. Print version also available.
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The use of JAK2 quantitative polymerase chain reaction for the diagnosis and monitoring of patients with chronic myeloproliferative diseasesWong, Ting-yan, Cybil. January 2008 (has links)
Thesis (M. Med. Sc.)--University of Hong Kong, 2008. / Includes bibliographical references (p. 61-65)
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Correlation of NIS mRNA levels with radioiodide uptake in mammary tumors and non-tumor mammary glands of MMTV-infected mice /Schworer, Stephen Andrew. January 2008 (has links)
Thesis (Honors)--College of William and Mary, 2008. / Includes bibliographical references (leaves 76-81). Also available via the World Wide Web.
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