Submitted by Diogo Misoguti (diogo.misoguti@gmail.com) on 2016-07-07T18:49:46Z No. of bitstreams: 1 cp078451.pdf: 1963747 bytes, checksum: 8df763273b9205acc5055fd0cbc3d714 (MD5) / Approved for entry into archive by Diogo Misoguti (diogo.misoguti@gmail.com) on 2016-07-07T18:50:39Z (GMT) No. of bitstreams: 1 cp078451.pdf: 1963747 bytes, checksum: 8df763273b9205acc5055fd0cbc3d714 (MD5) / Made available in DSpace on 2016-07-07T18:50:39Z (GMT). No. of bitstreams: 1 cp078451.pdf: 1963747 bytes, checksum: 8df763273b9205acc5055fd0cbc3d714 (MD5) Previous issue date: 2008 / Objetivo: Avaliar, do ponto de vista histológico, o efeito do transplante de tecido adiposo sobre o útero e os ovários de camundongas obesas e anovuladoras. Métodos: Foram utilizadas camundongas B6.V-Lepob/J com 2 e 3 meses de idade, subdivididas em grupos experimentais: 1.Controle (B6.V-Lepob/J fêmeas, com peso normal); 2.Obesidade (B6.V-Lepob/J fêmeas, obesas); 3.Obesidade + Transplante de tecido adiposo de camundongas doadoras com peso normal após 7 dias (B6.V-Lepob/J fêmeas e obesas, que receberam aproximadamente 1g de tecido adiposo de camundongas com peso normal isogênicas); 4.Obesidade + Transplante de tecido adiposo de camundongas doadoras com peso normal após 15 dias (B6.V-Lepob/J fêmeas e obesas, que receberam aproximadamente 1g de tecido adiposo de camundongas com peso normal isogênicas); 5.Obesidade + Transplante de tecido adiposo de camundongas doadoras com peso normal após 45 dias (B6.V-Lepob/J fêmeas e obesas, que receberam aproximadamente 1g de tecido adiposo de camundongas com peso normal isogênicas). Após 7, 15 e 45 dias do transplante, os animais foram sacrificados. Foram verificados o peso corporal e a glicemia, além da análise histológica dos úteros e dos ovários (H/E). Resultados e Conclusões: O grupo das obesas (n=6) apresentou elevação no peso corporal (52,0± 1,3*) e na glicemia (418,4±33,0*) em relação ao controle (24,0±0,7 e 100,2±2,2, respectivamente, n=5). Os transplantes após 7 dias (n=5) e 15 dias (n=9) foram eficazes na redução da glicemia (390,2±34,0 e 270,0±38,0*, pré e pós-Tx 7 dias; e 460,0±31,4 e 125,0±8,4*, pré e pós-Tx 15 dias, respectivamente), mas não modificaram o peso corporal. Porém, após 45 dias de transplante (n=7) houve redução da glicemia (467,0±28,0 e 209,0±18,0*, pré e pós-Tx, respectivamente), assim como tendência à diminuição no peso corpóreo observado pré e pós-Tx (50,4±2,4 e 44,0±3,3). Valores adotados para *p< 0,0001. A histologia revelou que, após 7 e 15 dias de Tx, os animais apresentavam ovários semelhantes aos das obesas, incluindo presença de inúmeros folículos, interstício abundante e ausência de corpos lúteos. Nos úteros notou-se intensa estimulação hormonal e glandular, mitoses, raros eosinófilos e epitélio superficial íntegro e sem renovação, sugerindo que tempos precoces de Tx são incapazes de reverter tais alterações. Entretanto, no grupo Tx 45 dias foram visualizados úteros praticamente iguais aos do controle, com grande número de leucócitos e remodelação do epitélio superficial, além de ovários com reduzida quantidade de folículos e presença de corpos lúteos, indicando ovulação. As camundongas B6.V-Lepob/J obesas apresentam formações polimicrocísticas e estroma abundante, mimetizando os achados ovarianos das pacientes anovuladoras. O Tx de tecido adiposo, a longo prazo, parece ter papel na diminuição da obesidade, mostrando-se eficiente na reversão da hiperglicemia e das alterações ovarianas e uterinas, presentes nas camundongas obesas, restabelecendo a ovulação. / Objective: To assess from the histological point of view the effect of adipose tissue transplantation onto the uterus and ovaries of obese, anovulating mice.en Method: B6.V-Lepob/J mice were used and the following experimental groups evaluated: 1.Control group (B6.V-Lepob/J female, average weight); 2.Obesity (B6.V-Lepob/J female, obese); 3.Obesity plus adipose tissue transplant from mice donors which were average weight after 7 days (B6.V-Lepob/J obese females which received adipose tissue from thin, isogenic mice); 4.Obesity plus adipose tissue transplant from mice donors which were average weight after 15 (B6.V-Lepob/J obese females which received adipose tissue from thin, isogenic mice); 5.Obesity plus adipose tissue transplant from mice donors which were average weight after 45 days (B6.V-Lepob/J obese females which received adipose tissue from thin, isogenic mice);. Within 7, 15 and 45 days of the transplant the animals were sacrificed. The age range was between 2 and 3 months old. In addition to the histological analyses of uteri and ovaries (H/E), these animals’ corporal weight and glycemia were verified. Results and Conclusion: The obese group (n=6) presented with elevation in corporal weight (52,0 ± 1,3*)and glycemia (418,4± 33,0*) in relation to the control group (24,0 ± 0,7 and 100,2± 2,2 respectively, n=5). The transplants after 7 days (n=5) and 15 days (n=9) were effective in reducing glycemia (390,2 ± 34,0 and 270,0 ± 38,0*, 7 days pre and post transplant; and 460,0 ± 31,4 and 125,0 ± 8,4*, 15 days pre and post transplant respectively), but not in changing corporal weight. However, within 45 days of the transplant (n=7) there was a reduction in glycemia (467,0 ± 28,0 and 209,0 ± 18,0*, pre and post- transplant respectively), as well as a tendency to reduce corporal weight observed pre and post transplant (50,4 ±2,4 and 44,0 ± 3,3). Values used for *p< 0,0001. Histology revealed that within 7 and 15 days of the transplant, the animals presented with ovaries similar to those in the obese group, containing countless follicles, abundant interstice and absence of corpora lutea. The uteri revealed intense hormonal and glandular stimulation, mitosis, scarse eosinophils and the superficial epithelium whole, without renovation, indicative of the inability of short term transplantations to reverse such alterations. In the 45-day-group, however, the uteri looked very much the same as those of the control group, with a great number of leucocytes and remodellation of the superficial epithelium, as well as ovaries with a reduced amount of follicles and presence of corpora lutea, suggesting ovulation. The obese B6.V-Lepob/J mice present with polymicrocystic formation and abundant oestrone, mimicking the ovarian findings of the anovulating patients. The long term adipose tissue transplantation, on the other hand, seems to play a part in reducing obesity, proving to be efficient in reversing hypoglycemia and the ovarian and uterine alterations present in obese mice, re-establishing ovulation