As variation in pork tenderness has increased, identification of tenderness has become an industry need. This study consisted of 1208 pork loins randomly selected to test the efficacy of four automated grading techniques. Visible and near-infrared spectroscopy (VVNIR) (350-1830 nm wavelengths), bioelectrical impedance (EI) (resistance, reactance, phase angle, and partial capacitance), pH, and CIE L*, a* and b* color space values were used to predict chemical moisture and lipid, pH, Warner-Bratzler shear force (WBSF), and Slice shear force (SSF) on 13 d aged pork loins. The means and standard deviations for WBSF were (22.95 and 5.16) and SSF were (165.49 and 58.15). Prediction was based on stepwise linear regression and partial least squares regression. VNIR, pH, and color, when in combination, had the highest R^2 (0.19 and 0.21) for the prediction of WBSF and SSF, respectively. Partial least squares regression (PLSR) was used to remove autocorrelation between VNIR values. By using PLSR, with an R ^2 value of 0.49, 100 percent of the "tender" chops were correctly classified, 93 percent of the "intermediate" chops were correctly classified, and 92 percent of the "tough" chops were correctly classified into its category for WBSF. However, SSF was much lower (R^2 = 0.24) with only correctly placing 62 percent of the "tender" chops and only 48 percent of the "intermediate" and "tough" chops. Electrical impedance, alone or in combination with other technologies, either did not improve predictability of linear regression equations (increase R^2) or of PLSR models (increase R^2). Equations and models that included EI values had low R^2. When adding EI to the regression equation involving all variables, R^2 increased slightly from 0.19 to 0.21 in predicting WBSF, and from 0.21 to 0.25 for SSF. When pH or CIE L* color space values were included in linear regression or PLSR models to predict WBSF and SSF, R^2 values increased from 0.14 to 0.19 for WBSF, and 0.14 to 0.21 for SSF. pH played a large role in predicting WBSF and SSF, along with CIE L*. Thus, for an on-line situation, use of VNIR, pH, and color could be used to predict tenderness. Utilization of VNIR alone could be effective in predicting pork tenderness (WBSF). Using EI alone, or in combination with VNIR, would not provide acceptable prediction of WBSF or SSF. Use of VNIR with pH and color would improve the ability to predict tender and intermediate pork WBSF and SSF, but the additional improvement in accuracy may not be warranted based on the cost and additional time needed when using more than one technology.

Graduation date: 1992

The objective of the study was to determine growth performance, physico-chemical attributes, oxidative stability and sensory quality of pork from pigs fed one of three dietary treatments, eac containing 0%, 2.5% or 5% (T1, T2 and T3, respectively) Moringa oleifera leaf meal (MOLM). Dietary treatments were formulated to be isonitrogenous and isoenergetic for weaner (6 – 8 weeks) and grower (9 – 13 weeks) phases. Twelve Large White (LW) and 12 Kolbroek (KB) male pigs at 6 weeks of age, initially weighing an average 10 kg, were randomly allocated to one of the dietary treatments, each with four replicates, in individual pens. Feed and water were offered ad libitum. The Longissimus thoracis et lumborum (LTL) muscle of each carcass (n = 24) was sampled for meat quality and fatty acid analyses. Breed differences in measured parameters were observed. In pigs receiving 5% MOLM, the LW had significantly the highest ADFI (P<0.05) than KB pigs. In pigs receiving 2.5% and 5% MOLM, backfat thickness was significantly highest (P<0.05) compared to those receiving 0% MOML. Dietary inclusion of MOLM had no significant effects (P>0.05) on the physico-chemical quality of pork from LW pigs; although it significantly (P<0.05) increased a* and reduced WBSF values in pork from KB pigs. Pork from LW pigs receiving 5% MOLM had significantly increased (P<0.05) n-3 content of the subcutaneous tissue of LW pigs compared to KB, and also when compread to other treatment groups. In both breeds, the composition of PUFA: SFA and n-6: n-3 in the subcutaneous adipose tissue was significantly (P<0.05) lower in T2 and T3 than in T1. The n-3 levels for pork muscle from LW pigs receiving 2.5% and 5% MOLM, and from KB pigs receiving 5% MOLM, were significantly (P<0.05) lower. Inclusions of MOLM significantly (P<0.05) reduced the n-6: n-3 fatty acids in pork from both LW pigs and KB pigs. Consumer scores on sensory attributes (aroma intensity, initial impression of juiciness, first bite, sustained impression of juiciness, muscle fibre and overall tenderness and overall flavor intensity) significantly (P<0.05) increased as the level of MOLM inclusion in the diet increased; and higher scores were observed in fried meat than in boiled for most sensory attributes. It may be concluded that inclusion of MOLM in pig diets up to 5% improved the ADFI, a*, tenderness of pork without adversely affecting the FCR and other physico-chemical quality attributes; and resulted in desired increase in levels of n-3 and reduced the n-6: n-3 fatty acid ratio.

No description available.

Once an animal is harvested for meat, skeletal muscle attempts to maintain ATP at or near antemortem levels. To maintain ATP levels postmortem, stored glycogen is catabolized to produce ATP through glycolysis and possibly oxidative metabolism. Hydrolysis of the produced ATP acidifies muscle until an ultimate pH is reached. The ultimate pH of meat directly impacts the quality characteristics of color, texture, and water holding capacity. Therefore, our research intends to describe the contributions glycolysis and oxidative metabolism play in determining ultimate pH and fresh meat quality. Traditionally, glycogen content at death was thought to be responsible for dictating ultimate pH. This was especially true in oxidative muscle with limited glycogen stores. Yet, our research indicated that in the presence of excess glycogen, oxidative muscle maintains a high ultimate pH. Rather, pH inactivation of phosphofructokinase is responsible for terminating postmortem glycolysis and brackets ultimate pH between 5.9 – 5.5. Meat with a pH below this range is uncommon. However, AMPK γ3R200Q mutant pigs produce meat with an ultimate pH near 5.3. Due to lower AMP deaminase abundance in their muscle, AMP levels are elevated late postmortem. Because AMP is a potent activator of phosphofructokinase, the aberrant meat quality from AMPK γ3R200Q mutant pigs is caused by extended postmortem glycolysis. Combined, these data further our understanding of the factors that contribute to the formation of fresh meat quality. We also characterized AMPK γ3R200Q muscle by investigating antemortem skeletal muscle lactate transport. Lactate is transported in or out of tissues by proton-linked iii monocarboxylate transporters (MCTs). Previous reports indicated that acute activation of AMPK increased monocarboxylate transporter expression in skeletal muscle of other species. Yet, it was unknown the impact chronic activation of AMPK will have on MCT1, MCT2, and MCT4 expression in pigs. Compared to wild-type pigs, the longissimus lumborum of AMPK γ3R200Q pigs increased both MCT2 and MCT4 protein expression. Our data suggest glycolytic skeletal muscle from the AMPK γ3R200Q pigs has increased capacity for antemortem lactate export from muscle and possibly increased pyruvate transport into the mitochondria. / Ph. D.

Commercial hams display variation in color uniformity across the cut surface, especially the semimembranosus (SM) muscle. This variation in fresh ham color, or two-toning, persists through further processing and contributes to production of a less desirable end product. In an attempt to understand the underlying source of this color variation, we evaluated the differences in muscle fiber-type composition and glycolytic metabolism in the SM muscle of fresh hams differing in color uniformity. Fifty-eight fresh SM muscles, ranging in color, were selected at 24 h postmortem and each partitioned into four distinct regions and three color classes based on color uniformity in the caudal region. The L* (lightness) values were greatest and a* (redness) values lowest in the most caudal portions of the muscle. The caudal portion also exhibited the lowest ultimate pH (P < 0.0001), lowest myoglobin (P < 0.05), greatest glycolytic potential (GP) (P < 0.0001) and the lowest myosin heavy chain type I isoform (P < 0.0001) abundance of all regions in 'normal' colored hams. After segregating based on L* values, the caudal region had identical pH, GP, LDH, and MyHC-I, despite significant differences in L* (P < 0.0001). These data show the most caudal aspects of the SM are indeed more prone to adverse postmortem metabolism and suggest that inherent differences in muscles of the ham may make some areas of the ham more vulnerable to temperature abuse during harvesting. / Master of Science

No description available.

The study was conducted to determine the effect of pre-slaughter stress of pigs on the levels of cortisol, creatine kinase (CK) and their subsequent relationship with pork quality. From our knowledge, there is paucity of information on transportation, lairage duration and sex effects on saliva cortisol and its relation with serum cortisol, urine cortisol and other meat quality traits. Stage one of the study assessed the effect of sex and time to slaughter (transport and lairage duration) on the levels of cortisol and CK in crossbred pigs. The second stage of the study evaluated the effect of sex and lairage duration on pork quality. The trials comprised of 60 22-weeks old commercial crossbred pigs [(30 boars vs. 30 gilts) with an average body weight of 76.9kg for gilts and 78.3kg for boars. Thirty pigs (15 Boars vs. 15 Gilts) were slaughtered on arrival after a 120km road journey and the other 30, after travelling the same road and distance, were slaughtered after having been in the lairage for 20 hours. Saliva samples were obtained during three time periods: between 07:00-10:00 on the day before the journey, on arrival at the abattoir and after lairage. Levels of cortisol in serum and urine and CK in serum samples collected at slaughter were determined. The samples (saliva and serum) were centrifuged at 20oC for 10 min at 3550 x g and stored at -20oC in separate vials until analysis. Pork quality was assessed using muscle pH, colour, thawing and cooking losses and Warner-Bratzler Shear Force. Correlations between cortisol levels in saliva, serum and urine and meat quality were assessed. The effect of sex and time to slaughter on saliva cortisol was significant. Statistical analysis showed significant interaction of sex by time to slaughter on serum cortisol. It was only the effect of sex that demonstrated higher (P <0.05) serum creatine kinase levels in gilts. Likewise, urine cortisol was influenced (P <0.05) only by time to slaughter. Saliva cortisol after transportation (r = 0.52) was correlated (P <0.05) with urine cortisol. The study suggests that there are greater responses of the Hypothalamic-pituitary-adrenal axis following time to slaughter (transport and lairage duration) between gilts and boars. This can be used in measuring cortisol in saliva, serum and urine, and serum creatine kinase to determine stress levels in pigs. Besides the lightness (L*) colour value which was significantly lower (P <0.05) in meat from gilts than from boars, the results of this study showed no interaction or individual effect (sex or lairage duration) on pH and other colour measurements. Meat cooking loss was affected (P <0.05) by gender x lairage interaction. Effect of lairage duration (as an individual effect) on the measured meat quality traits was not significant (P <0.05). Correlation analysis showed significant (P <0.05) negative relation between baseline saliva cortisol (r = -0.40) and the pork L* value. Saliva cortisol after lairage duration (r = -0.38 and r = 0.38) was correlated (P <0.05) with pH45 and pork a* value, respectively. Serum cortisol (r = -0.35) was negatively correlated (P <0.05) with pH45. Moreover, there were negative correlations (P <0.05) between saliva cortisol after transportation (r = -0.35), saliva cortisol after lairage duration (r = -0.44), serum cortisol (r = -0.40) and meat cooking loss. The study suggests that cortisol in saliva and serum can be used to determine effect of time to slaughter (transport and lairage duration) on meat quality traits from gilts and boars. Keywords: Boars; gilts; saliva cortisol; serum cortisol; serum creatine kinase; urine cortisol.

Master of Science / Department of Animal Sciences and Industry / Terry A. Houser / Two experiments were conducted to determine the effects of dried distillers grains with solubles (DDGS) on pork loin and fat quality. In the first experiment, 1,160 barrows (PIC) were used in a 70-d study to determine the influence of DDGS and glycerol on pork loin and fat quality attributes. Barrows were fed a corn-soybean meal based diet with the addition of selected levels of DDGS (0 or 20%) and glycerol (0, 2.5, or 5%) feed stuffs. Loins from the two heaviest pigs in each pen were removed for evaluation of pork loin and fat quality. Experiment two was a pilot study, in which eight non-pregnant sows were fed either 0 or 50% DDGS with a corn soybean meal based diet for 92-d. In the first experiment, there were no DDGS x glycerol interactions for purge loss %, instrumental color (L*a*b*), visual color, marbling score, drip loss %, visual color, pH, Warner-Bratzler shear force (WBSF), cook loss %, myofibrillar tenderness, juiciness, pork flavor intensity, connective tissue amount, and overall tenderness. There was a DDGS x glycerol interaction (P<0.03) for off-flavor intensity. Pigs fed diets with 20% DDGS had higher WBSF values, lower myofibrillar tenderness, lower overall tenderness scores, lower connective tissue scores, and had more off-flavors (P<0.05). Loin fatty acid analysis revealed an increase in palmitoleic, linoleic, and eicosadienoic acids (P<0.05) and iodine value (P<0.03) for pigs fed 20% DDGS. In the second experiment, there were no differences (P>0.64) in BW or backfat change for sows fed either 0 or 50% DDGS. No differences (P>0.23) in lipid oxidation from lean trimmings as measured by 2-thiobarbituric acid reactive substances (TBARS) assay were reported either initially or after 5 d of retail display for sows fed either 0 or 50% DDGS. As expected, lipid oxidation increased (P<0.003) as measured by TBARS assay for both treatments from d 1 to 5. Jowl fatty acid analysis revealed an increase in linoleic acid (P<0.01), total polyunsaturated fatty acids (P<0.01), and the ratio of polyunsaturated fatty acids to saturated fatty acids (P<0.03) for sows fed 50% DDGS.

Thesis (MScAgric)--University of Stellenbosch, 2005. / ENGLISH ABSTRACT: Porcine somatotropin (pST) is a naturally occurring protein (hormone), secreted by the pituitary gland of young pigs and is one of the major growth regulating factors. High levels of pST is found in circulating blood of young animals, resulting in the partitioning of nutrients into lean tissue and bone growth. Supplying an exogenous source of pST should increase the deposition of lean muscle and bone and decrease the deposition of fat in the older (above 60 kg) pig. To ascertain whether pST would have a positive influence on production- and meat characteristics in the South African scenario for pigs slaughtered at a high bodymass, a trail was conducted. For group housed animals pST had no significant effect on the following parameters: feed intake, calculated cumulatively on a weekly basis, ADG, live weight, carcass weight, carcass length, ham length or chest depth, intramuscular fat area, muscle depth and colour measured with a Hennessey probe and waterbinding capacity. However, when the FCR of pigs in this investigation were calculated, there was a significant (p<0.05) influence by sex and pST detected. Boars converted their feed to live weight better than barrows and gilts from week ten onwards. Boars had an increased FCR when treated with pST. A significant increase was found in muscle area and a significant decrease in extra muscular (back fat) area of boars and barrows. A significant pST (p< 0.05) effect (3 mm reduction) was seen for backfat depth measured by the Hennessey probe and the intrascope. Porcine somatotropin significantly (p<0.05) increased the muscle area of the loin-cut for all animals. The area covered by subcutaneous fat of boars and barrows were significantly (P<0.05) reduced by pST treatment, with no effect detected for gilts (p>0.05). Porcine somatotropin treatment increased the muscle percentage and decreased the extramuscular fat percentage in such a way that the differences between sexes was reduced. Thus, more uniform fat-muscle distribution between carcasses was obtained by pST treatment. Control animals had a significantly higher pH24 than pST treated animals (P=0.049). Lower values were found for animals receiving pST for L* (p=0.016), a* (p=0.002) and b* (P=0.016). The effect on b* (yellow-blue range) in the M longissimus thoracis of pST treated animals showed slightly (but significantly) less yellow and more green compared to control animals (p=0.016). This combined with the lower L* values (brightness) indicates that pST treated animals had a significantly darker colour meat compared to the control animals. Individually housed animals showed no significant differences for the following characteristics: live weight, carcass weight, head, trotters, shoulder, middle back, middle belly, loin belly, thigh, fillet, carcass fat and kidney. Whereas pST caused a significantly lower percentage of the middle back of boars and barrows, but not in gilts, pST could only precipitate a lower percentage (11.18%) loin back of treated animals (p=0.026) v.s. control animals (12.05%). A trend (p>0.1) was detected for percentage bone in the middle back, with the pST treated (14.17% vs. 13.18%) animals having more bone than that of control animals. pST animals had a higher percentage (p=0.024) skin (5.04%) than the control animals (4.28%). This study shows that there is no negative effect of pST on meat quality characteristics and carcass composition, in fact there is less variation between carcasses obtained from different sexes treated with pST. The producer can bring heavier animals to the market with a reduced backfat percentage and a greater percentage meat with the help of pST. / AFRIKAANSE OPSOMMING: Vark somatotropien (pST) is ‘n natuurlike hormoon wat deur die pituitêre klier in die brein afgeskei word by klein varkies en is een van die belangrikste hormone betrokke by groei regulering. Hoë vlakke van pST kom voor in die bloed van jong varkies, dit veroorsaak dan die verspreiding van nutriente in die liggaam van die varkie sodat dit meer vleis en beengroei toon en minder vet deponeer. Namate die varkie volwasse word neem die bloedvlakke van pST af en begin die liggam meer vet deponeer ten koste van proteien groei, terselfde tyd begin die seksuele kenmerke ontwikkel. Die toediening van ‘n eksterne bron van pST behoort die groei van been en vleis te bevoordeel in ouer diere (bo 60 kg). Vir die doeleindes van die ondersoek wou ons bepaal of pST ‘n positewe effek het op groei en vleiskwaliteitseienskappe van varke wat in Suid Afrikaanse kondisies gebruik word en teen ’n hoër liggamsmassa as gewoonlik geslag word. Vir varke wat in groepe behuis was was daar geen effek op die volgende eienskappe nie: voer inname weekliks bepaa, gemiddelde daaglikse toename, liggaamsmassa, karkasgewig, karkas lengte, ham lengte, bors diepte, intrmuskulêre vet en spierdikte bepaal met ‘n Hennessey sonde asook waterbindigs vermoë. Bere het egter ’n beter voeromsettings faktor gehat as burge en soggies, maar as pST toegedien is het hulle voeromsettingsfaktor toegeneem. ’n Betekenisvolle (p<0.05) toename in spier area van alle diere, met ’n gesamentlike afname in onderhuidse vet area van bere en burge (nie soggies nie) is gevind. ’n Betekenisvolle afname (3 mm) in rugvetdikte is gevind by diere wat met pST behandel is. Hierdie effekte is so in die lewe gebring dat die verskil tussen die geslagte minder prominent is en karkasse meer uniform is. Betekenisvolle hoër pH24 waardes is gevind by kontrole diere as by pST behandelde diere (p=0.049). L* (p=0.016), a* (p=0.002) en b* (p=0.016) waardes was betekenisvol laer vir pST behandelde diere as vir kontrole diere. Die effek op b* waardes (geel-blou reeks) in die M. longissimus thoracis van behandelde diere was in so ‘n mate dat die vleis ietwat minder geel en meer groen was in vergelyking met die kontrole diere (p=0.016), saam met laer L* waardes (helderheid) is ‘n indikasie van ietwat donkerder vleis van behandelde diere. Individueel behuisde diere het geen betekenisvolle effek getoon vir die volgende parameters nie: liggamsgewig, karkasgewig, kop, voete, skouer, middel rug, middel maag, lende maag, dy, haas en niere. ‘n Betekenisvolle laer persentasie middel rug is gevind in bere en burge, maar nie vir soggies nie, maar in die lende rug is ‘n betekenisvolle effek gevind vir alle diere (11.8% vir pST en 12.05% vir kontroe , p=0.026). ‘n Neiging (p>0,1) is gesien vir die hoeveelheid been in die middle rug van diere behandel met pST (14.17% vs. 13.18%) Dier met pSt behandel het’n betekenisvol hoer persentasie vel as kontrole diere gehat (5.04% vs. 4.28%, p=0.024. Die resultate van die ondersoek bewys dat daar geen negatiewe effekte van pST op vleis- en karkaseienskappe is nie, daar is self minder variasie tussen karkasse van verskillende geslagte. Die boer kan swaarder diere bemark met minder rugvet en meer vleis met behulp van pST.