Genetic diversity of two salmonid species, Dolly Varden (Salvelinus malma) and coastal cutthroat trout (Oncorhynchus clarki clarki) in Prince William Sound, Alaska were examined at multiple spatial scales with three molecular markers. Pleistocene glaciers covered what is now Prince William Sound 8,000- 12,000 years ago and both species colonized the region subsequently. Because these species have different migratory behavior and historic ranges I expected to see different patterns of genetic diversity within Prince William Sound. Haplotype frequency and nucleotide diversity in sixteen mitochondrial DNA (mtDNA) restriction fragment enzymes combinations in Prince William Sound were higher in Dolly Varden than in coastal cutthroat trout, which were close to fixation. Low estimates of these measures in coastal cutthroat trout may reflect a founder effect resulting from colonization of this region from a single glacial refuge (Cascadia) and low dispersal capacity. To examine if genetic diversity in coastal cutthroat trout was higher in areas of glacial refuge, three additional locations from throughout their distributional range were examined with the same restriction enzymes. Haplotype frequency and nucleotide diversity were lower in areas that were glaciated than estimates of the same measures in a glacial refuge. Again, founder effects during colonization of the three glaciated sites may account for these results. Genetic variation of Dolly Varden and coastal cutthroat trout was also examined within and among populations in Prince William Sound with microsatellites and allozymes. The pattern of genetic variation in coastal cutthroat trout was complex. There was lower genetic diversity within populations that were recently colonized following recent de-glaciation (150-350 ybp). Genetic diversity among some coastal cutthroat trout populations was high, which possibly reflects restricted migration. In other trout populations there was low diversity among populations, possible reflecting historic gene flow. In Dolly Varden, genetic variation among anadromous populations in Prince William Sound was low. There were large differences among resident and anadromous populations. These data provide information for the management for both species, which may reduce the risk of the loss of genetic diversity within local populations. / Graduation date: 2003

No description available.

Breeding habitat of Harlequin ducks (Histrionicus histrionicus) was studied in eastern Prince William Sound, Alaska, during 1991 - 1993. Streams in Prince William Sound were surveyed for Harlequin ducks and monitored with mist nets. Physical characteristics of 24 Harlequin breeding streams were compared to those of 24 streams not used for breeding using 2 sample, principal components and logistic regression analyses. Nests were located using radio-telemetry of marked females. Harlequin ducks resident in eastern Prince William Sound selected the largest anadromous salmon streams available for nesting. Volume discharge of breeding streams averaged 3.2 m��/s and was the most important factor in habitat variation between streams used and not used by breeding Harlequins. Expansive estuaries and intertidal deltas present at the outflow of large streams were important foraging and loafing areas of Harlequin ducks. Although nesting females generally avoided smaller salmon streams their intertidal estuaries were often used for foraging by females and molting males. The largest streams in Prince William Sound, glacially fed rivers, were not used by breeding Harlequins. Ten nest sites of Harlequin ducks in eastern Prince William Sound were located on southwest facing, steeply sloping banks of small, first order tributaries near timberline elevation. Nests were associated with woody debris and shrubs, in shallow depressions or cavities, and were beneath the canopy of old growth forest. Microhabitat produced by a southwest aspect, snow shadow provided by the forest canopy, and sloping stream bank may provide nesting sites earlier in the spring compared to surrounding areas. / Graduation date: 1994

I investigated the relationship between harlequin duck (Histrionicus histrionicus) behavior and lack of recovery from the Exxon Valdez oil spill in Prince William Sound, Alaska. First, I evaluated the hypothesis that harlequin ducks in winter have little flexibility to increase foraging time in response to disturbance because they are constrained to forage during daylight. Eight radio-tagged harlequin ducks wintering in Resurrection Bay, Alaska were monitored for evidence of dive-feeding at night. Each radio-tagged individual was detected during an average of 19.5 of 22 nocturnal monitoring sessions and signal loss indicative of diving behavior was not detected during a total of 780 minutes of signal monitoring. In contrast, the same 8 radio-tagged birds were detected during an average of 9.1 of 12 daytime signal monitoring sessions and signal loss indicated diving behavior during an average of 62 �� 7% of 5-minute daytime monitoring periods (total of 364 minutes of signal monitoring). Thus the harlequin ducks monitored in this study rarely, if ever, fed by diving at night, possibly due to reduced foraging efficiency and (or) increased predation risk at night. This result suggests that harlequin ducks in mid-winter may be severely time-limited in their foraging, especially in northern parts of their winter range. Therefore, subtle changes in energy requirements and (or) time-activity budgets as a result of continued exposure to residual oil from the Exxon Valdez oil spill may affect the ability of harlequin ducks to meet their daily energy requirements. Second, I tested the hypothesis that exposure to crude oil affects time-activity budgets of harlequin ducks. Controlled oil-dosing and plumage-oiling experiments were conducted using adult female harlequin ducks in captivity. I found no evidence that ingestion of weathered Prudhoe Bay crude oil affected the occurrence of feeding activity during 30-minute observation periods, nor was there evidence of effects on time spent feeding. Effects of crude oil ingestion on maintenance activity were detected, but were neither consistent between the 2 years of the study, nor dose-dependent for the 2 doses administered (2 and 20 mL kg����� wk�����), and therefore did not strongly support an oil-dosing effect on maintenance activity. Consequently, these results provided little support for the hypothesis that oil ingestion affects time-activity budgets of captive harlequin ducks, at least for the doses and conditions of captivity used in this study. Plumage-oiling reduced feeding activity in captive harlequin ducks. The estimated probability of feeding during 30-minute observation periods for birds in the high-exposure oiling group (5 mL of crude oil) was 53% less than that of non-oiled controls. Oiled birds exhibited a trend of reduced time feeding with increasing level of external-oiling; this effect was greatest among birds in the high-exposure oiling group, which spent 43% less time feeding than non-oiled birds. Reduced feeding was associated with less time in the water dive-feeding and presumably lower heat loss. Trends in the occurrence of maintenance activity and time spent in maintenance activity for birds in the high-exposure treatment suggested plumage-oiling increased maintenance activity, but results were not conclusive. The behavioral changes associated with plumage-oiling in captivity would likely reduce fitness in the wild, where a high proportion of time must be spent in the water feeding. If residual Exxon Valdez oil sequestered in beach sediments enters the water column where it may be encountered by harlequin ducks, external exposure may lead to reduced feeding activity. This, in turn, may compromise survival, particularly during mid-winter when the time available for diurnal foraging is low and maintenance energy requirements are high. / Graduation date: 2004

Recent studies indicate a 70% decrease in the Alaskan Steller sea lion (SSL) population (ca. 5% per year) since the early 1980's. In accordance with a 1997 status classification of the Western Steller sea lion (WSSL) stock as endangered, the "critical habitat" for the species was to be defined. This habitat has now been designated to include 10-20 nautical mile buffer zones around most rookeries and haulouts in the Gulf of Alaska (GOA) and Aleutian Islands. However, these zones were based on limited, summer, foraging data. The primary objective of this study was to characterize juvenile SSL diving behavior and habitat use along the Kenai Peninsula and Prince William Sound (PWS) from winter to spring. Fifteen free ranging, subadult SSL of both sexes were captured and equipped with satellite telemeters at five haulout sites in PWS and Resurrection Bay, Alaska. Telemeters transmitted for an average of 122 days (range 38-181 days). A total of 11,692 locations were received and 217,419 dives recorded. All sea lions exhibited localized movements parallel or close to shore (3-15 km offshore). Young of the year (YOY) exhibited high site fidelity. Older juvenile sea lion lions were less restricted in their movements and traveled greater distances (200-400km) visiting a variety of islands, buoys, and other locations in PWS. Most dives were short (mean duration = 1.1 min) and shallow (mean depth = 10.8 m), with animals diving to an average maximum depth of 193 m. During winter (January and February), many dives (>40%) occurred during the daytime (0900-1500 LT). However, by April and May this pattern shifted and the animals made most of their dives (>40%) during the night (2100-0300 LT). This relationship was more pronounced for dives deeper than 20 m and coincided with the seasonal increase in photoperiod. Subadult SSL, especially YOY, remained within the 20 nautical mile coastal zone during winter and spring. Shallow, nearshore waters provide important habitat during this critical period of transition to nutritional independence. However, more conclusive data on SSL foraging ecology is necessary to better understand locations and depths preferred by the species.

Thesis (M.S.)--Oregon State University, 2007. / Printout. Includes bibliographical references (leaves 140-144). Also available on the World Wide Web.

This study was a replication of Fitzpatrick and Rubin's study of response strategies used in crisis communication. It differed though, in the type of crisis analyzed. While Fitzpatrick and Rubin studied cases of sexual harassment, this study examined oil spills of two supertanker groundings. Both the Exxon Valdez and the Sea Empress groundings, and especially Acomarit and Exxon, the companies responsible were examined by using content analysis. Units of analysis were statements by each company's spokesperson covered in either four American or four Norwegian newspapers. These statements were then categorized as one of four response strategies defined by Fitzpatrick and Rubin which were as follows: (1) The traditional public relations strategy, (2) The traditional legal strategy, (3) Mixed strategy, and, (4) Diversionary strategy.On behalf of these response strategies the study stated two hypotheses:H1: The spokespersons of Acomarit used the traditional public relations strategy significantly more than the traditional legal strategy, as defined by Fitzpatrick and Rubin, in Norwegian newspapers.The hypothesis was accepted.H2: The spokespersons of Exxon used the traditional legal strategy significantly more than the traditional public relations strategy, as defined by Fitzpatrick and Rubin, in American newspapers.The hypothesis was partially accepted.The study used an American and a Norwegian coder to secure reliability. Holsti's coder reliability test yielded eighty percent for the American statements, and seventy percent for the Norwegian statements.The study also indicated that the traditional public relations strategy was the most used response strategy. This was different to what Fitzpatrick and Rubin found in their study. They found the traditional legal strategy was used the most in cases of sexual harassment.However, both Exxon and Acomarit used the traditional public relations strategy more than the traditional legal strategy, but differed significantly in their use of mixed strategy and diversionary strategy. Exxon was found to have used the diversionary strategy significantly more than Acomarit. The researcher indicated that this may have been one of the contributing factors which lead to Exxon's public relations nightmare. In addition, Exxon was found to have used more spokespeople than Acomarit, which in turn may have made it difficult to centralize the information flow. / Department of Journalism

Range, areas of concentrated activity, and dispersal characteristics for juvenile Steller sea lions Eumetopias jubatus in the endangered western population (west of 144° W in the Gulf of Alaska) are poorly understood. This study quantified space use by analyzing post-release telemetric tracking data from satellite transmitters externally attached to n = 65 juvenile (12-25 months; 72.5 to 197.6 kg) Steller sea lions (SSLs) captured in Prince William Sound (60°38'N -147°8'W) or Resurrection Bay (60°2'N -149°22'W), Alaska, from 2003-2011. The analysis divided the sample population into 3 separate groups to quantify differences in distribution and movement. These groups included sex, the season when collected, and the release type (free ranging animals which were released immediately at the site of capture, and transient juveniles which were kept in captivity for up to 12 weeks as part of a larger ongoing research program). Range-use was first estimated by using the minimum convex polygon (MCP) approach, and then followed with a probabilistic kernel density estimation (KDE) to evaluate both individual and group utilization distributions (UDs). The LCV method was chosen as the smoothing algorithm for the KDE analysis as it provided biologically meaningful results pertaining to areas of concentrated activity (generally, haulout locations). The average distance traveled by study juveniles was 2,131 ± 424 km. The animals mass at release (F[subscript 1, 63] = 1.17, p = 0.28) and age (F[subscript 1, 63] = 0.033, p = 0.86) were not significant predictors of travel distance. Initial MCP results indicated the total area encompassed by all study SSLs was 92,017 km², excluding land mass. This area was heavily influenced by the only individual that crossed over the 144°W Meridian, the dividing line between the two distinct population segments. Without this individual, the remainder of the population (n = 64) fell into an area of 58,898 km². The MCP area was highly variable, with a geometric average of 1,623.6 km². Only the groups differentiated by season displayed any significant difference in area size, with the Spring/Summer (SS) groups MCP area (Mdn = 869.7 km²) being significantly less than that of the Fall/Winter (FW) group (Mdn = 3,202.2 km²), U = 330, p = 0.012, r = -0.31. This result was not related to the length of time the tag transmitted (H(2) = 49.65, p = 0.527), nor to the number of location fixes (H(2) = 62.77, p = 0.449). The KDE UD was less variable, with 50% of the population within a range of 324-1,387 km2 (mean=690.6 km²). There were no significant differences in area use associated with sex or release type (seasonally adjusted U = 124, p = 0.205, r = -0.16 and U = 87, p = 0.285, r = -0.13, respectively). However, there were significant differences in seasonal area use: U = 328, p = 0.011, r = -0.31. There was no relationship between the UD area and the amount of time the tag remained deployed (H(2) = 45.30, p = 0.698). The kernel home range (defined as 95% of space use) represented about 52.1% of the MCP range use, with areas designated as "core" (areas where the sea lions spent fully 50% of their time) making up only about 6.27% of the entire MCP range and about 11.8% of the entire kernel home range. Area use was relatively limited – at the population level, there were a total of 6 core areas which comprised 479 km². Core areas spanned a distance of less than 200 km from the most western point at the Chiswell Islands (59°35'N -149°36'W) to the most eastern point at Glacier Island (60°54'N -147°6'W). The observed differences in area use between seasons suggest a disparity in how juvenile SSLs utilize space and distribute themselves over the course of the year. Due to their age, this variation is less likely due to reproductive considerations and may reflect localized depletion of prey near preferred haul-out sites and/or changes in predation risk. Currently, management of the endangered western and threatened eastern population segments of the Steller sea lion are largely based on population trends derived from aerial survey counts and terrestrial-based count data. The likelihood of individuals to be detected during aerial surveys, and resulting correction factors to calculate overall population size from counts of hauled-out animals remain unknown. A kernel density estimation (KDE) analysis was performed to delineate boundaries around surveyed haulout locations within Prince William Sound-Kenai Fjords (PWS-KF). To closely approximate the time in which population abundance counts are conducted, only sea lions tracked during the spring/summer (SS) months (May 10-August 10) were chosen (n = 35). A multiple state model was constructed treating the satellite location data, if it fell within a specified spatiotemporal context, as a re-encounter within a mark-recapture framework. Information to determine a dry state was obtained from the tags time-at-depth (TAD) histograms. To generate an overall terrestrial detection probability 1) The animal must have been within a KDE derived core-area that coincided with a surveyed haulout site 2) it must have been dry and 3) it must have provided at least one position during the summer months, from roughly 11:00 AM-5:00 PM AKDT. A total of 10 transition states were selected from the data. Nine states corresponded to specific surveyed land locations, with the 10th, an "at-sea" location (> 3 km from land) included as a proxy for foraging behavior. A MLogit constraint was used to aid interpretation of the multi-modal likelihood surface, and a systematic model selection process employed as outlined by Lebreton & Pradel (2002). At the individual level, the juveniles released in the spring/summer months (n = 35) had 85.3% of the surveyed haulouts within PWS-KF encompass KDE-derived core areas (defined as 50% of space use). There was no difference in the number of surveyed haulouts encompassed by core areas between sexes (F[subscript 1, 33] << 0.001, p = 0.98). For animals held captive for up to 12 weeks, 33.3% returned to the original capture site. The majority of encounter probabilities (p) fell between 0.42 and 0.78 for the selected haulouts within PWS, with the exceptions being Grotto Island and Aialik Cape, which were lower (between 0.00-0.17). The at-sea (foraging) encounter probability was 0.66 (± 1 S.E. range 0.55-0.77). Most dry state probabilities fell between 0.08-0.38, with Glacier Island higher at 0.52, ± 1 S.E. range 0.49-0.55. The combined detection probability for hauled-out animals (the product of at haul-out and dry state probabilities), fell mostly between 0.08-0.28, with a distinct group (which included Grotto Island, Aialik Cape, and Procession Rocks) having values that averaged 0.01, with a cumulative range of ≈ 0.00-0.02 (± 1 S.E.). Due to gaps present within the mark-recapture data, it was not possible to run a goodness-of-fit test to validate model fit. Therefore, actual errors probably slightly exceed the reported standard errors and provide an approximation of uncertainties. Overall, the combined detection probabilities represent an effort to combine satellite location and wet-dry state telemetry and a kernel density analysis to quantify the terrestrial detection probability of a marine mammal within a multistate modeling framework, with the ultimate goal of developing a correction factor to account for haulout behavior at each of the surveyed locations included in the study. / Graduation date: 2013

The pigeon guillemot (Cepphus columba) population in Prince William Sound has failed to recover from declines that occurred both before and after the Exxon Valdez Oil Spill (EVOS). Post-spill studies of pigeon guillemot breeding biology have identified three potential factors limiting recovery: (1) predation on eggs and nestlings; (2) declines in the proportion of high-lipid, schooling forage fish (sand lance [Ammodytes hexapterus], herring [Clupea pallasi], and capelin [Mallotus villosus]) in the diet; and (3) continued exposure to residual oil from the spill. This laboratory study with captive-reared pigeon guillemots at the Alaska SeaLife Center investigated two aspects of the species' biology that are relevant to restoration in the aftermath of EVOS. First, we investigated the role of dietary factors (prey type, quantity of food consumed, dietary fat content, and energy intake rate) in limiting the growth, development, survival, and fledging condition of nestling pigeon guillemots. The objective was to understand how changes in prey availability and prey quality might affect pigeon guillemot productivity. Second, we fed nestlings sublethal doses of weathered Prudhoe Bay crude oil (PBCO) and then measured several potential biomarkers of effects from this pollutant. These dose-response experiments were designed to (1) better understand the impact on nestling guillemots of petroleum hydrocarbons in food, (2) calibrate existing and potential biomarkers of exposure to PBCO in pigeon guillemots in a controlled, laboratory setting, and (3) develop better nondestructive biomarkers of exposure to PBCO in pigeon guillemots in particular, and seabirds in general. Results of feeding experiments indicated that most variation in nestling growth rates could be explained by variation in daily energy intake. The type of forage fish consumed, the lipid or protein content of the forage fish, and even the quantity of food consumed daily did not have as strong an effect on nestling guillemot growth as did daily energy intake. The metabolic efficiency and growth performance of nestling guillemots was not enhanced on high-lipid diets, contrary to results with nestlings of some other seabird species. Instead, structural growth (wing length) in nestling guillemots was somewhat stunted on high-lipid diets. These attributes of guillemot nutritional requirements are associated with the guillemots' nearshore foraging niche and high food provisioning rates to nestlings. The average lipid content of sand lance, juvenile herring, and capelin may represent the optimal dietary lipid content for nestling pigeon guillemots. This study supports the hypothesis that guillemot productivity is limited by the availability of these forage fishes through effects on energy provisioning rates to nestling guillemots. Consequently, recovery of pigeon guillemot populations injured by EVOS is likely linked to recovery of these key forage fish stocks. Results of the oil-dosing experiments indicated that nestling guillemots are resistant to small doses of weathered PBCO in their food. No nestlings died or suffered noticeable health effects following dosing. The high dose in this study (0.5 ml kg����� day�����) was sufficient to induce hepatic cytochrome P450A1 (a liver enzyme indicative of contaminant exposure), but growth rate, fledging mass, and blood chemistry were largely unaffected. None of the 12 plasma or hematological markers examined responded in a dose-dependent manner to ingestion of weathered PBCO, except lactate dehydrogenase (LDH). Although baseline stress hormone (corticosterone) levels were not different between oil-dosed and control nestlings, a standardized acute stress protocol revealed that corticosterone was more elevated during stress for oiled nestlings compared to controls. Although we were not successful in identifying a noninvasive biomarker (e.g., growth) or a blood biomarker (e.g., haptoglobin) of crude oil exposure in nestlings, we were able to confirm that levels of hepatic cytochrome P4SO1AI and corticosterone during stress were elevated by the sublethal doses administered during our experiments. Based on this and other studies, it is unlikely that the failure of pigeon guillemots to recover from EVOS is due to effects on nestling health of residual oil in food. / Graduation date: 2003