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Engineering a protein for peptide detection and allosteric activationLewis, Marsha Jane, 1970- 06 October 2010 (has links)
Strategies for the engineering of allosteric proteins, which are proteins that bind ligands at a specific site other than the reaction site and affect the reaction activity, are still being perfected. There have been allosteric proteins successfully engineered based on the hypothesis that the two allosterically related sites are distinct, modular domains and use trial and error to construct and test novel protein domain fusions for allostery.
This work uses laboratory evolution to engineer the peptide binding affinity of the protein binding domain of the allosteric E. coli protease DegS. The protein binding domain is a PDZ domain (named for Postsynaptic density protein (PSD-95), Discs-large protein (Dlg), and Zonula occludens-1 (ZO-1)) that binds the C-terminus of unfolded outer membrane porins. Combinatorial libraries of PDZ domain variants were displayed anchored to the periplasmic membrane of E. coli. The cells were permeabilized and incubated with fluorescent peptide ligands. PDZ domains were screened by flow cytometry for binding to the target peptide ligands. The PDZ domain binding affinity was improved by 20-fold for the peptide ligand that represents the physiological ligand; and the PDZ domain binding affinity was expanded to accommodate a negatively charged residue in a novel peptide ligand. The E. coli anchored peripalsmic expression (APEx) methodology in conjunction with flow cytometry had not previously been used to modify the binding affinity of a PDZ domain.
The selected PDZ domain variants were then fused to the wild-type DegS protease domain and analyzed to determine if allosteric activation was made more sensitive to the native ligand or altered to respond to the novel peptide ligand. Interestingly, the DegS fusion protein with the PDZ variant containing the most subtle mutations retained a degree of allostery for the physiological peptide ligand and obtained a degree of allostery for the novel activating peptide ligand. Other selected PDZ variants with additional and expected mutations in the ligand binding site did not respond allosterically to the peptide ligands and the respective DegS fusions were constitutively active, suggesting that the amino acid network linking the allosteric binding event to protease activity is intricately integrated. / text
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Studies of disulphide mutants of barnaseClarke, Jane January 1993 (has links)
No description available.
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Protein SupplementationSprinkle, Jim 11 1900 (has links)
7 pp.
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Protein SupplementationSprinkle, Jim E. 12 1900 (has links)
Revised; Originally Published: 2000 / 6 pp.
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Folding and stability studies on papain and the effect of recombinant papain pro fragmentBriggs, Geoffrey Shaw January 1994 (has links)
No description available.
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Folding and assembly studies on the components of mammalian PDC and OGDCMcCartney, Richard Graham January 1998 (has links)
No description available.
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Improving detection of promising unrefined protein docking complexesRörbrink, Malin January 2016 (has links)
Understanding protein-protein interaction (PPI) is important in order to understand cellular processes. X-ray crystallography and mutagenesis, expensive methods both in time and resources, are the most reliable methods for detecting PPI. Computational approaches could, therefore, reduce resources and time spent on detecting PPIs. During this master thesis a method, cProQPred, was created for scoring how realistic coarse PPI models are. cProQPred use the machine learning method Random Forest trained on previously calculated features from the programs ProQDock and InterPred. By combining some of ProQDock’s features and the InterPred score from InterPred the cProQPred method generated a higher performance than both ProQDock and InterPred. This work also tried to predict the quality of the PPI model after refinement and the chance for a coarse PPI model to succeed at refinement. The result illustrated that the predicted quality of a coarse PPI model also was a relatively good prediction of the quality the coarse PPI model would get after refinement. Prediction of the chance for a coarse PPI model to succeed at refinement was, however, without success.
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An evaluation of aspects of the PEM (protein energy malnutrition) Scheme for malnourished children in Gauteng ProvinceMarshall, Carol Anne 21 May 2014 (has links)
This study assessed aspects of targeting of the PEM scheme in Gauteng province. This
food supplementation scheme targets beneficiaries including children 0-6 years, using
largely anthropometric criteria. Routine provincial intervention data was analysed and
related to available data cm expenditure, population and indices of socio-economic
need. Only 28% were children in the critical 7-36 month age group; and coverage was
very low, with no correlation between indices of need and programme implementation
by area. An exit interview to a sample of caretakers of 0-6 year old children in four
clinics (two Local Authority, two provincial) examined the process of identification of
beneficiaries. Low attendance by children over 1 year and 40% errors in growth
monitoring, more frequent among sick children, effectively reduced screening
coverage among the most at-risk. Health worker misclassification resulted in an 81%
exclusion error among those meeting entry criteria, while 4% of the total were wrongly
enrolled. Advice and nutrition promotion to caregivers was inadequate.
Recommendations include service re-organisation, community-based initiatives and
better monitoring.
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Interplay of YB-1 between tubulin and mRNA / Interaction de YB-1 avec la tubuline et l'ARN messagerChernov, Konstantin Grigorievich 05 December 2008 (has links)
YB-1 est un régulateur important de l’expression des gènes dans les cellules eucaryotes. En plus de son rôle dans la transcription, YB-1 joue un rôle clé dans la traduction et la stabilisation des ARN messagers. Nous avons identifié plusieurs nouveaux partenaires de la protéine YB-1 par chromatographie d’affinité à partir de différents extraits tissulaires. Parmi ces partenaires, nous avons démontré que YB-1 interagit avec la tubuline et les microtubules et stimule fortement l'assemblage des microtubules in vitro. Les microtubules assemblés en présence de YB-1 ont une ultrastructure normale, et les données montrent que YB-1 recouvre probablement la surface extérieure des microtubules. De la même façon YB-1 stimule aussi l'assemblage de la tubuline-MAP qui est plus proche des complexes protéiques qui existent dans la cellule, et de la tubuline clivée par subtilisine ce qui suggère que son interaction avec la tubuline ne relève pas seulement d’effets électrostatiques. Nous avons enfin découvert que la tubuline interfère avec la formation des complexes ARNm:YB-1. Ces résultats suggèrent que YB-1 peut réguler l'assemblage des microtubules in vivo et que son interaction avec la tubuline peut contribuer à la régulation de la traduction des ARN messagers. En effet, in vivo, la traduction des mRNPs dépend de l’état de saturation de l’ARN messager par YB-1. Nous avons montré ici que lorsque le rapport YB-1:ARNm est faible, les complexes mRNPs possèdent des structures non-compactes, alors que les mRNPs saturés sont compacts. Ce changement structural est observé de façon parallèle à l'inhibition de la traduction des ARN messagers lorsqu’ils passent des polysomes (traduits) aux mRNPs libres (non traduits). De façon intéressante, nous avons découvert que les mRNPs saturés se lient aux microtubules via des interactions protéine:protéine et ont tendance à former des agrégats sur la surface des microtubules. Cette dernière propriété pourrait contribuer à la formation de granules de stress et à la localisation des mRNPs dans le cytoplasme. Finalement, un modèle de diffusion facilité a été développé pour expliquer l'assemblage des microtubules orchestré par les polyamines naturelles (telles que YB-1 qui sont positivement chargées dans la cellules). L’ensemble de ces données contribuent à une meilleure compréhension de processus biologiques fondamentaux concernant l’assemblage de la tubuline en microtubules et le trafic des ARN dans la cellule. Ils pourraient avoir un intérêt pour développer de nouveaux médicaments qui ciblent les microtubules. / YB-1 is a major regulator of gene expression in eukaryotic cells. In addition to its role in transcription, YB-1 plays a key role in translation and stabilization of mRNAs. We identify several novels YB-1 protein partners by affinity chromatography of different tissue extracts. We observed that YB-1 interacts with tubulin and microtubules and stimulates microtubule assembly in vitro. Microtubules assembled in the presence of YB-1 exhibited a normal single wall ultrastructure where YB-1 probably coats the outer microtubule wall. Furthermore, we found that YB-1 also promotes the assembly of MAPs-tubulin and subtilisin-treated tubulin. Additionally, we demonstrated that tubulin interferes with mRNA:YB-1 complexes. These results suggest that YB-1 may regulate microtubule assembly in vivo and that its interaction with tubulin may contribute to the control of mRNA translation. The translational status of mRNPs in vivo depends on amount of YB-1 associated with mRNA. We show here that at low YB-1:mRNA ratios mRNP complexes possess an incompact structures, whereas saturated mRNPs are compact. This structural change corresponds to translation inhibition when mRNA moves from polysomal (translatable) to free (untranslatable) mRNPs. Saturated mRNPs bind to microtubules via protein:protein interactions and tend to self-aggregate on microtubule surface. This property could contribute to stress granule formation, mRNPs traffic and localization of translation apparatus within cytoplasm. Finally, the facilitated diffusion model was developed to explain enhancement of microtubule assembly by positively charged natural polyamines in living cells. Altogether our data contribute to the understanding of fundamental biological processes.
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Double-click stapled peptides for inhibiting protein-protein interactionsLau, Nathan Yu Heng January 2015 (has links)
No description available.
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