Spelling suggestions: "subject:"1protein dinteractions"" "subject:"1protein d'interactions""
11 |
Sex hormone-binding globulin protein-protein interactions and identification of a novel isoform /Ng, Kwong-man. January 2006 (has links)
Thesis (Ph. D.)--University of Hong Kong, 2007. / Title proper from title frame. Also available in printed format.
|
12 |
The mechanism of interaction of the linker histone with DNA and nucleosomesEllen, Thomas Patrick 27 June 2003 (has links)
This dissertation examines the interaction of the linker histone with DNA and with
nucleosomes. The first goal of the project was to characterize the interaction of the
linker histone with DNA. Three factors previously reported to influence the linker
histone's interaction with DNA were examined: ratio of linker histone to DNA sites
of binding, monovalent ions in the local environment, and conformation of the DNA
molecules. Evidence obtained through gel mobility shift assays demonstrates the
strong preference by the linker histone for DNA with superhelical torsion, i.e.,
supercoiling, and the negative cooperative mode of binding that the linker histone
exhibits in association with supercoiled DNA.
The second part of the dissertation examines the location of linker histone binding
on the nucleosome, and documents the pronounced tendency of the linker histone to
bind to two DNA duplex strands. A preparation of homogeneous nucleosome core
particles, consisting of a defined 238 base pair DNA fragment and the core histone
octamer positioned precisely on this DNA, was used as a substrate for the UV-induced
crosslinking of the linker histone to the DNA of this nucleosome. By site-specific
labeling of a single site on the DNA of the nucleosome, the linker histone was
observed crosslinked at that labeled site, confirming that the linker histone binds at the
pseudo-dyad axis of the nucleosome. This evidence was used to support a model of
linker histone binding to the nucleosome that invokes the association of the linker
histone with no fewer than two duplex strands of DNA of the nucleosome. / Graduation date: 2004
|
13 |
Binding and assembly of H5 (and the globular domain of H5) onto DNACarter, George John 07 January 1998 (has links)
In order to better characterize linker histone interactions with DNA, avian erythrocyte-specific linker H5 and the trypsin-resistant globular domain of H5 (GH5) were used in DNA binding studies. To begin, H5 displayed a considerably higher binding
affinity for DNA than the isolated globular domain (GH5), supporting the importance of the terminal tail domains in binding. This conclusion is based upon binding curves conducted in low-salt solution, and on the considerably-higher salt concentration required
to prevent protein-DNA contact. Linker histones also induce DNA-protein aggregation in a process that was found to result in protein insolubility in 2% SDS, and included protein-protein interactions that did not require the terminal tail domains. In addition, DNA supercoiling appeared to impede the aggregation process; this that may be attributable to binding of linker histones in isolated clusters, as gauged by a limit in the number of observed dithiobis (succinimidyl) propionate (DSP)-crosslinkable contacts. In a related study, the property of GH5 to bind, then organize onto DNA was investigated. GH5 crosslinked onto DNA with dithiobis (succinimidyl propionate), then cleaved with chymotrypsin, displayed highly uniform contacts that appeared to involve the C-terminal four amino acids, and suggests protein-protein interactions are important for binding. This finding may be relevant since GH5 (and H5) were observed to self-associate free in solution in an arguably specific manner. Finally, the exposure of Phe 93 to chymotrypsin was used to identify the surface of the globular domain that contacts DNA for the binding
of intact H5. Results suggests that the side of the protein opposite to the recognition helix preferentially binds to DNA, supporting a novel winged-helix protein DNA-binding mechanism.
Furthermore, parallel studies with octamers reconstituted onto a DNA fragment with twelve copies of the 208 b.p. rDNA 5s gene from Lytechinus variegatus, shows that H5 had a high binding affinity with all detectable protein binding to the reconstituted complex. H5 binding conferred protection to a site located near the dyad axis from endonuclease digestion, supporting the contention that H5 binds near or at the nucleosome dyad axis. H5 binding also was observed to condense fibers as observed from agarose gel electrophoresis, although velocity analytical sedimentation studies indicate that H5 in itself was not sufficient to fully compact chromatin fibers; rather H5 and 30 mM NaCl, in combination, were required. Results indicate that the chromatin-reconstituted "208-12 DNA" makes an excellent model for analyzing the effect of linker proteins on chromatin morphology. / Graduation date: 1998
|
14 |
Protein-DNA interactions molecular modeling and energetics /Plaxco, Kevin W., Goddard, William A., January 1994 (has links)
Thesis (Ph. D.)--California Institute of Technology, 1994. UM #94-06,216. / Advisor names found in the Acknowledgements pages of the thesis. Title from home page. Viewed 01/15/2010. Includes bibliographical references.
|
15 |
The topology and geometry of DNA and DNA-protein interactions /Buck, Dorothy E. January 2001 (has links)
Thesis (Ph. D.)--University of Texas at Austin, 2001. / Vita. Includes bibliographical references (leaves 110-115). Available also in a digital version from Dissertation Abstracts.
|
16 |
Functional studies of mouse quaking protein /Wu, Jiang, January 2001 (has links)
Thesis (Ph. D.)--University of Texas at Austin, 2001. / Vita. Includes bibliographical references (leaves 135-149). Available also in a digital version from Dissertation Abstracts.
|
17 |
Targeting protein-protein interactions with fragment-based approachesStubbs, Christopher James January 2013 (has links)
No description available.
|
18 |
The use of long wavelength fluorescence in the study of ligand-protein interactionsBrown, Marc B. January 1993 (has links)
The binding of a drug or other ligand to plasma proteins can effect their absorption, metabolism and excretion which can lead to a change in its toxicity and therapeutic action. Fluorescence is a technique that has been used to study such interactions and has the advantages of extreme sensitivity and specificity. Previously fluorescence has been monitored in the UV /vis range of the spectrum. However, a new development is long wavelength fluorescence (600-1000nm), which has the added benefits of a lower background, decreased scattering, decreased photodecomposition and the availability of inexpensive, solid state, optical components. Certain dyes including polymethines, xanthenes and phenoxazines that fluoresce in the long wavelength region (600-1000nm) of the spectrum were investigated for use as fluorescence probes. Nile Red, a strongly hydrophobic phenoxazine dye, was found to have an emission wavelength and intensity which was strongly dependent on the polarity of its environment. Consequently, it was bound to certain proteins including bovine and human serum albumin, aI-acid glycoprotein and B-lactoglobulin and provided both qualitative and quantitative information on the nature and type of binding site on the protein. It was also used in the study of ligand protein binding interactions in which competition for a binding site on the protein occurs between the probe and other ligands such as drugs or fatty acids. The project also involved a preliminary investigation into a novel double probe technique for the study of drug-protein interactions and the development of a flow injection analysis method involving gradient titration of a drug against a probe:protein system.
|
19 |
Protein interactions and phase behavior in aqueous solutions effects of salt, polymer, and organic additives /Dumetz, Andre C. January 2008 (has links)
Thesis (Ph.D.)--University of Delaware, 2007. / Principal faculty advisor: Abraham M. Lenhoff, Dept. of Chemical Engineering, and Eric W. Kaler, College of Engineering . Includes bibliographical references.
|
20 |
Adenovirus and its interaction with host cell proteins /Carr, Sharon. January 2007 (has links)
Thesis (M.Phil.) - University of St Andrews, March 2007.
|
Page generated in 0.1064 seconds