Thesis (Ph. D.)--University of Chicago. / Includes bibliographical references (p. 14-15).

Academisch proefschrift--Amsterdam. / Summary in English: p. 91-93. "Litteratuurlijst": p. [94]-96.

Thesis (PH. D.)--University of Chicago. / Bibliography: p. 14-15. Also available on the Internet.

Thesis (PH. D.)--University of Chicago, 1919. / "Private edition, distributed by the University of Chicago Libraries, Chicago, Illinois." "Reprinted from the Journal of infectious diseases, Vol. 24, No. 5, May, 1919." Bibliography: p. 54-56.

Methylglyoxal synthetase, which catalyzes the formation of methylglyoxal and inorganic phosphate from dihydroxyacetone phosphate, was found in extracts of Proteus vulgaris. An efficient purification procedure utilizing ion exchange column chromatography and isoelectric focusing has been developed. Homogeneity of the enzyme preparation was confirmed by polyacrylamide gel electrophoresis and rechromatography.Two components of methylglyoxal synthetase were obtained upon isoelectric focusing. A comparison of the chemical and physical properties of the two components was carried out. The enzyme is a dimer. In the presence of inorganic phosphate, the hyperbolic saturation kinetics with dihydroxyacetone phosphate are shifted to sigmoidal.

Thesis (Ph. D.)--Illinois State University, 1973. / Title from title page screen, viewed Oct. 14, 2004. Dissertation Committee: William L. Daniel (chair), Herman E. Brockman, David F. Weber, Arlan Richardson, Howard Hetzel. Includes bibliographical references (leaves 53-66) and abstract. Also available in print.

Thèse (M.Sc.)--Université Laval, 2007. / Bibliogr.: f. 105-109. Publié aussi en version électronique dans la Collection Mémoires et thèses électroniques.

Les séquences d'insertion sont des parasites moléculaires d'ADN codant uniquement pour leur machinerie de transposition et sont retrouvées majoritairement dans les génomes et les plasmides des procaryotes. Le séquençage génomique massif de la dernière décennie nous a permis de détecter chez des souches bactériennes cliniques plusieurs gènes de résistance aux antibiotiques associés aux séquences d'insertion sous forme de transposons composés. Il a été suggéré que les séquences d'insertion jouent un rôle prépondérant dans l'évolution bactérienne et qu'elles possèdent un pouvoir recruteur permettant de sélectionner, réorganiser et propager des gènes conférant un avantage sélectif à l'hôte. Nous avons partiellement caractérisé le potentiel recruteur de deux séquences d'insertion associées à des gènes de résistance, la séquence IS26 de Proteus vulgaris et la séquence ISJO de Salmonella typhimurium, en observant les étapes de recrutement d'un gène chromosomique et leurs distributions épidémiologiques en consultant les banques de données. / Insertion sequences are DNA molecular parasites encoding exclusively their transposition function, and are mainly found within the genomes and plasmids of prokaryotic organisms. The massive genomic sequencing we have witnessed in the last decade has allowed us to detect in clinical bacterial isolates many antibiotic resistance genes associated with insertion sequences in the form of composite transposons. It has been suggested that insertion sequences may act as a primary modulator of bacterial evolution, and that they possess a recruitment capacity allowing them to select, reorganize and spread genes encoding adaptation functions for their hosts. We have partially characterized the recruitment potential of two insertion sequences well known for their association with antibiotic resistance genes, IS10 from Salmonella typhimurium and 1S26 from Proteus vulgaris, by observing the steps involved in the recruitment process of a chromosomal gene and their epidemiological distribution by consulting various databanks.

Programa de Apoio aos Planos de Reestruturação e Expansão das Universidades Federais / The microbiota intestinal of Psittaciformes is mainly composed of Gram positive bacteria, is considered a sign of illness the presence of Gram negative bacteria. Wild birds are important to public health by harboring pathogens able of zoonotic transmission. The displacement of these birds, such as occurs in the trafficking of wild animals, is a propagation mechanism of new endemic foci of infections agents to long distances from where they were acquired. In this study we examined the presence of enterobacteria in psittacines using samples of organs of birds received for necropsy at the Laboratório Central de Diagnóstico de Patologias Aviárias (LCDPA) situate in the Departamento de Medicina Veterinária Preventiva (DMVP) at Centro de Ciências Rurais (CCR) of the Universidade Federal de Santa Maria (UFSM). We also collected samples using swabs of cloaca and crop, in live birds, kept in captivity in order to monitor the possible presence of pathogenic bacteria. Escherichia coli was considered the predominant bacteria in most samples, then after microbiological analysis and confirmation of E. coli isolates, we selected 28 colonies, which were tested for virulence factors iss, this gen is associated with the strain s ability to resist the lytic effects of serum, and iutA an outer membrane receptor of involved in the high affinity binding of iron-aerobactin. It is hoped that the data obtained in this research will help in establishing the health status of psittacines helping maintain the species and preventing risks to public health. / A microbiota intestinal de Psitaciformes é composta principalmente por bactérias Gram positivas, não se considerando saudável a presença de bactérias Gram negativas. As aves silvestres possuem importância para a saúde pública por albergarem patógenos passíveis de transmissão zoonótica. O deslocamento territorial dessas aves, como o que ocorre no tráfico de animais selvagens, constitui um mecanismo de propagação de novos focos endêmicos de agentes infecciosos a grandes distâncias dos locais onde foram adquiridos. Neste trabalho analisamos a presença de enterobactérias em psitacídeos utilizando amostras de órgãos de aves recebidas para necropsia na rotina do Laboratório Central de Diagnóstico de Patologias Aviárias (LCDPA) situado no Departamento de Medicina Veterinária Preventiva (DMVP) do Centro de Ciências Rurais (CCR) da Universidade Federal de Santa Maria (UFSM). Também foi realizada coleta de suabes cloacais e de inglúvio em aves vivas mantidas em cativeiro visando monitorar a presença de possíveis bactérias patogênicas. Escherichia coli foi considerada a bactéria predominante na maioria das amostras, então após análise microbiológica e confirmação dos isolados de E. coli, selecionamos 28 colônias, as quais foram testadas para os fatores de virulência iss, relacionado com a capacidade da cepa em resistir aos efeitos líticos do soro, e iutA, sendo este gene um receptor de membrana externa dos compostos sideróforos da aerobactina (genes iuc) que é um sistema de captação de ferro pelo qual E. coli expressa afinidade. Espera-se que os dados obtidos nessa pesquisa contribuam no estabelecimento do estado sanitário de psitaciformes auxiliando na manutenção da espécie e prevenindo riscos à saúde pública.