Unternehmensvorstellung Parametric Technology und Roadmap PTC Mathcad Prime 3.0

In diesem Vortrag wird Mathcad Prime 2.0 gezeigt. Es wird ein Ausblick auf die Anbindung zu anderen PTC Produkten gegeben und auch ein Blick auf die Mathcad Roadmap geworfen.

Adaptor proteins play a vital role in the propagation of cellular signals. Although they lack endogenous catalytic activity, they contain a variety of protein binding modules, which enable them to promote specific and efficient interactions with their binding partners. They form integrative platforms for a variety of molecules (e.g. lipids, tyrosine kinases, cytoskeletal and signaling proteins), and thereby link and coordinate key functions such as cell growth, motility and shape determination. Our laboratory has recently cloned a novel, 130 kDa adaptor protein, named XB130, as a structural homolog of actin-filament-associated-protein (AFAP-110), a stress fiber-binding Src substrate. However, the molecular interactions and functions of this novel adaptor remained to be elucidated. To characterize the function of XB130 we asked two general questions: (1) Is XB130 involved in the signal transduction pathways of tyrosine kinases? And (2) Is XB130 capable of regulating the cytoskeleton and/or is it regulated by the cytoskeleton? To address these questions first we investigated the tissue distribution of XB130 and discovered that it is abundantly expressed in thyroid. Therefore we asked whether it is a target of the thyroid-specific tyrosine kinase, RET/PTC, a genetically rearranged, constitutively active enzyme that plays a pathogenic role in papillary thyroid cancer. We found that XB130 is a RET/PTC substrate that couples RET/PTC signaling to phosphatidylinositol 3-kinase (PI3K) activation through its phosphorylation dependent interaction with the regulatory subunit p85 of PI3K. XB130 plays an important role in PI3K signaling, as downregulation of XB130 in TPC1 papillary thyroid cancer cells, harboring the RET/PTC1 kinase, strongly reduced Akt activity and concomitantly inhibited cell cycle progression and survival in suspension. In the second part we demonstrate that XB130 is a novel Rac- and cytoskeleton-regulated protein that exhibits high affinity to lamellipodial (branched) F-actin and impacts motility and invasiveness of tumor cells. In conclusion, my work characterized a novel adaptor protein and assigned two well-defined pathophysiological functions to it in the context of thyroid cancer cells.

Adaptor proteins play a vital role in the propagation of cellular signals. Although they lack endogenous catalytic activity, they contain a variety of protein binding modules, which enable them to promote specific and efficient interactions with their binding partners. They form integrative platforms for a variety of molecules (e.g. lipids, tyrosine kinases, cytoskeletal and signaling proteins), and thereby link and coordinate key functions such as cell growth, motility and shape determination. Our laboratory has recently cloned a novel, 130 kDa adaptor protein, named XB130, as a structural homolog of actin-filament-associated-protein (AFAP-110), a stress fiber-binding Src substrate. However, the molecular interactions and functions of this novel adaptor remained to be elucidated. To characterize the function of XB130 we asked two general questions: (1) Is XB130 involved in the signal transduction pathways of tyrosine kinases? And (2) Is XB130 capable of regulating the cytoskeleton and/or is it regulated by the cytoskeleton? To address these questions first we investigated the tissue distribution of XB130 and discovered that it is abundantly expressed in thyroid. Therefore we asked whether it is a target of the thyroid-specific tyrosine kinase, RET/PTC, a genetically rearranged, constitutively active enzyme that plays a pathogenic role in papillary thyroid cancer. We found that XB130 is a RET/PTC substrate that couples RET/PTC signaling to phosphatidylinositol 3-kinase (PI3K) activation through its phosphorylation dependent interaction with the regulatory subunit p85 of PI3K. XB130 plays an important role in PI3K signaling, as downregulation of XB130 in TPC1 papillary thyroid cancer cells, harboring the RET/PTC1 kinase, strongly reduced Akt activity and concomitantly inhibited cell cycle progression and survival in suspension. In the second part we demonstrate that XB130 is a novel Rac- and cytoskeleton-regulated protein that exhibits high affinity to lamellipodial (branched) F-actin and impacts motility and invasiveness of tumor cells. In conclusion, my work characterized a novel adaptor protein and assigned two well-defined pathophysiological functions to it in the context of thyroid cancer cells.

Submitted by Barroso Patrícia (barroso.p2010@gmail.com) on 2014-08-21T01:51:35Z No. of bitstreams: 1 GESTEIRA, Mária de Fátima Malvar.pdf: 14332589 bytes, checksum: fab60e995789cf4073cfcc1575ba30b2 (MD5) / Made available in DSpace on 2014-08-21T01:51:35Z (GMT). No. of bitstreams: 1 GESTEIRA, Mária de Fátima Malvar.pdf: 14332589 bytes, checksum: fab60e995789cf4073cfcc1575ba30b2 (MD5) / Durante o preparo químico-cirúrgico do canal radicular, a efetividade dos instrumentos endodônticos e das soluções químicas auxiliares empregadas durante a modelagem, limpeza e desinfecção sustentam o sucesso do tratamento endodôntico. Ao seu lado, agentes quelantes têm sido utilizados ao final da instrumentação com o objetivo de remover da superfície dentinária um extrato de aparência amorfa, superfície irregular e granulosa, denominada camada de magma dentinário. Este estudo se propôs a avaliar, in vitro, por meio da Microscopia Eletrônica de Varredura, o efeito do EDTA a 17% / NaOCl 1% e do BioPure™ MTAD® na remoção da camada de magma dentinário, em dentes preparados química e cirurgicamente com o auxílio de Endo-PTC® / NaOCl 1% e de NaOCl 1%. Incisivos e caninos superiores humanos unirradiculares (n=50) foram distribuídos por sorteio em quatro Grupos Experimentais (n=10/grupo) e dois Grupos Controle Positivo (n=5/grupo). Nos grupos Experimentais 1 e 3 e no Grupo Controle Positivo 1, a instrumentação realizou-se com o auxílio da associação Endo- PTC® / NaOCl a 1%. Nos Grupos Experimentais 2 e 4 e no Grupo Controle Positivo 2, a instrumentação realizou-se com NaOCl 1%. Concluída a instrumentação, procedeu-se à irrigação final, nos Grupos Experimentais 1 e 2, com 10 mL de EDTA a 17% / NaOCl 1% por 1 minuto e, nos Grupos Experimentais 3 e 4, com BioPure™ MTAD® conforme orientação do fabricante. Em seguida, os dentes foram clivados, e a limpeza da superfície dentinária apical foi avaliada através da Microscopia Eletrônica de Varredura. Após tratamento estatístico, o resultado deste estudo revelou que as soluções de EDTA a 17% / NaOCl 1% mostraram-se mais eficazes na remoção da camada de magma dentinário no terço apical radicular do que o BioPure™ MTAD® (p<0,05), concluindo-se, porém, que nenhuma das substâncias testadas foi capaz de remover toda a camada de magma dentinário nessa região. O creme Endo-PTC® não contribuiu, do ponto de vista estatístico, para dificultar a remoção da camada de magma dentinário. A 6 mm do ápice, as superfícies dentinárias tratadas com EDTA a 17% / NaOCl a 1% apresentaram superfícies dentinárias mais limpas do que a 3 mm.

In this project a number of peptidyl transferase antibiotics were studied, specifically a group of aminohexose cytosine nucleoside antibiotics and their interaction with a selected number of highly conserved ribonucleic acid (RNA) motifs, designed to represent their possible binding site within the ribosome. This group of antibiotics shows a wide range of interesting properties, including antiviral and anti-tumour activity, and as they bind to a particularly conserved region in the ribosome, they are likely to be difficult for microorganisms to develop resistance to. It is hoped that once the mechanism of action of these antibiotics is better understood, that modifications to the antibiotics can be effectively made to create new or hybrid antibiotics with more selective antibacterial, or indeed antiviral or anti-tumour properties. The nuclear magnetic resonance (NMR) structure of the RNA binding, peptidyl tranferase inhibitor antibiotics amicetin, blasticidin S and gougerotin, in their native solution states, have been successfully determined. The structures all exhibit a stable conformation, stabilised by intramolecular hydrogen bonds. Amicetin was observed to be folded, distinctly different from the linear, extended conformation of amicetin previously determined by X-ray crystallography. The structure of blasticidin S was found to be very similar to its X-ray crystal structure. Gougerotin was shown to form a similar conformation to blasticidin S, save that the end chain of gougerotin was bent at right angles to the rest of the molecule, forming a structure similar to that of the major bound X-ray crystal structure of blasticidin S. All the solution structures showed a similar conformation in the analogous regions of their chemical structure, suggesting that hybrid antibiotics could be produced.Two highly conserved RNA motifs of Halobacterium halobium (H. h.) and Escherichia coli (E. coli) 23S ribosomal RNAs were chosen to investigate their interaction with amicetin. The NMR structure of the H. h. and E. coli. 29-mer RNA motifs have been determined; the motifs both form well folded A-form RNA conformations. The E. coli NMR structure differs from the X-ray crystal structure of the motif contained within the ribosome, as a highly conserved adenine residue, which resides in a bulge strongly implicated with amicetin binding, folds into the helix as opposed to being flipped out. Instead, an adjacent cytosine residue partially flips out; whereas in the crystal structure, it is folded within the helix. The NMR stuctures of the H. h. motif differs from the X-ray crystal structure of the motif, contained within the ribosome, as none of the bases are flipped out and a number of non-canonical base pairs are formed in the solution structure. To continue this study, a fully 13C and 15N isotopically labelled version of the H. h. RNA sample has been partially assigned, and an initial structure determination has been performed, using ultra high field 1 GHz spectroscopy.Addition of amicetin to both the H. h. and E. coli 29-mer RNA samples were accompanied by discrete changes to the spectra, suggesting weak interaction between the two components. These can be qualitatively interpreted to changes induced in the local conformation of the RNA motifs and the amicetin arising from the formation of a complex, between the amicetin and the bulge region of the particular motif.

Gegenstand der vorliegenden Publikation sind Festigkeitsberechnungen von Maschinen aus der Hütten- und Walzwerkstechnik mit Pro/Mechanica. Erläutert werden die Arbeitstechniken, die bei der Aufbereitung der Modelle zum Einsatz kommen, sowie die genutzten Regelwerke für den Festigkeitsnachweis. Die angeführten Rechenbeispiele stammen allesamt aus der täglichen Praxis der industriellen Nutzung von FE-Methoden im Maschinenbau.

Gerenderte Animation von kinematisch gekoppelten Komponenten mit Pro/ENGINEER bzw. Creo zur Produktpräsentation oder auch Montageanleitung

Einsatzmöglichkeiten, Neuheiten und Ausblick auf kommende Releases von Mathcad Prime

Die neuen Fertigungsmöglichkeiten durch die Additive Fertigung ermöglicht es nicht nur topologisch neuartige Bauteile herzustellen, sondern auch Bauteile mit inneren Strukturen zu versehen, die der Bauteilbelastung angepasst sind oder anderen Funktionen Freiräume bieten. Ein Ansatz ist es durchlässige innere Strukturen, z. B. Gitterstrukturen (auch als Lattice Strukturen bezeichnet) einzusetzen und durch die damit geschaffenen großen inneren Flächen eine effiziente Bauteilkühlung zu realisieren. Anhand eines einfachen Beispiels wird durch Simulation und Experiment die Wirkung einer solchen Kühlung gezeigt. Als weiteres Anwendungsbeispiel wird der Einsatz verschiedener innere Strukturen zur festigkeitsgerechten Gestaltung gewichtsoptimierter Bauteile vorgestellt. In beiden Fällen wird die Gestaltung mit Hilfe von FE-Modellen experimentell begleitet. / The new manufacturing possibilities offered by additive manufacturing not only allows to produce topologically novel components, but also enables to provide components with internal structures that are adapted to the component load or offer new possibilities for other functions. One approach is to use permeable internal structures, e. g. lattice structures, to realize efficient component cooling through the large internal surfaces created thereby. The effect of such a cooling is demonstrated by simulations and experiments using a simple example. As a further application example, the use of various internal structures for the strength-oriented design of weight-optimized components will be presented. In both cases the design is experimentally accompanied by FE models.