Yam Wing Sze. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2005. / Includes bibliographical references (leaves 154-160). / Abstracts in English and Chinese. / Abstract English --- p.i / Chinese --- p.iii / Acknowledgments --- p.v / Table of contents --- p.vii / List of Tables --- p.x / List of Figures --- p.xi / List of Abbreviations --- p.xvi / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Chinese Medicine and Western Medication --- p.1 / Chapter 1.2 --- Chinese Medicine and Compound Formula --- p.2 / Chapter 1.3 --- Cardiovascular disease (CVD) and atherosclerosis --- p.6 / Chapter 1.4 --- General Research Objectives --- p.19 / Chapter Chapter 2 --- Establishment of compound formulation and Extract Preparation --- p.21 / Chapter 2.1 --- Formulation searched from Chinese Pharmacopoeia --- p.21 / Chapter 2.2 --- Aqueous extract preparation --- p.25 / Chapter 2.2.1 --- Materials and Methods --- p.25 / Chapter 2.2.2 --- Discussion --- p.27 / Chapter Chapter 3 --- Vasodilation study --- p.28 / Chapter 3.1 --- Vascular Smooth Muscle Contraction and Relaxation --- p.28 / Chapter 3.2 --- Endothelium and Vasodilation --- p.30 / Chapter 3.3 --- Vasodilation in organ bath --- p.32 / Chapter 3.3.1 --- Materials and Methods --- p.32 / Chapter 3.3.2 --- Results --- p.35 / Chapter 3.3.3 --- Discussion --- p.40 / Chapter 3.4 --- Endothelium dependent vasodilation --- p.40 / Chapter 3.4.1 --- Materials and Methods --- p.43 / Chapter 3.4.2 --- Results --- p.45 / Chapter 3.4.3 --- Discussion --- p.54 / Chapter 3.5 --- Adrenoceptor and vasodilation --- p.55 / Chapter 3.5.1 --- Materials and Methods --- p.57 / Chapter 3.5.2 --- Results --- p.58 / Chapter 3.5.3 --- Discussion --- p.62 / Chapter 3.6 --- Potassium Channels and Vasodilation --- p.63 / Chapter 3.6.1 --- Materials and Methods --- p.65 / Chapter 3.6.2 --- Results --- p.67 / Chapter 3.6.3 --- Discussion and Summary --- p.77 / Chapter 3.7 --- Potential active components from Fenge and Danshen --- p.82 / Chapter 3.7.1 --- Materials and Methods --- p.82 / Chapter 3.7.2 --- Results --- p.83 / Chapter 3.7.3 --- Discussion --- p.87 / Chapter Chapter 4 --- Comparison of Fenge and Yege --- p.88 / Chapter 4.1 --- Vasodilative effects of Fenge and Yege --- p.89 / Chapter 4.1.1 --- Materials and Methods --- p.89 / Chapter 4.1.2 --- Results --- p.89 / Chapter 4.1.3 --- Discussion --- p.101 / Chapter 4.2 --- The comparison of antioxidative effect between Yege and Fenge --- p.104 / Chapter 4.2.1 --- Red blood cell hemolysis model --- p.106 / Chapter 4.2.1.1 --- Materials and Methods --- p.106 / Chapter 4.2.1.2 --- Results --- p.108 / Chapter 4.2.1.3 --- Discussion --- p.110 / Chapter 4.2.2 --- Ischemia-reperfusion on Langendroff --- p.112 / Chapter 4.2.2.1 --- Materials and Methods --- p.114 / Chapter 4.2.2.2 --- Results --- p.117 / Chapter 4.2.2.3 --- Discussion --- p.125 / Chapter Chapter 5 --- Comparison of Chemical Profiles of Fenge and Yege --- p.127 / Chapter 5.1 --- The application of HPLC --- p.127 / Chapter 5.2 --- HPLC standardization --- p.129 / Chapter 5.2.1 --- Materials and Methods --- p.132 / Chapter 5.2.2 --- Results --- p.133 / Chapter 5.2.3 --- Discussion --- p.144 / Chapter Chapter 6 --- "Summaries, Discussion and prospects" --- p.146 / Chapter 6.1 --- Summaries and Discussion --- p.146 / Chapter 6.2 --- Prospects --- p.148 / Chapter 6.2.1 --- "Cardiovascular tonic effect of pure compounds, extracts with difference solvents and their vasodilative mechanism." --- p.148 / Chapter 6.2.2 --- Macrophage Foam Cell and Atherosclerosis --- p.149 / Chapter 6.2.3 --- The D:F (7:3) and D:Y (7:3) compound formulae capsule with GMP --- p.152 / References --- p.154

Submitted by Cleide Dantas (cleidedantas@ufpa.br) on 2014-07-29T12:11:30Z No. of bitstreams: 2 license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) Dissertacao_ValorNutritivoLeguminosa.pdf: 723204 bytes, checksum: 6ec8237d127a836feed2314b3a253275 (MD5) / Approved for entry into archive by Ana Rosa Silva (arosa@ufpa.br) on 2014-08-28T17:09:08Z (GMT) No. of bitstreams: 2 license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) Dissertacao_ValorNutritivoLeguminosa.pdf: 723204 bytes, checksum: 6ec8237d127a836feed2314b3a253275 (MD5) / Made available in DSpace on 2014-08-28T17:09:08Z (GMT). No. of bitstreams: 2 license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) Dissertacao_ValorNutritivoLeguminosa.pdf: 723204 bytes, checksum: 6ec8237d127a836feed2314b3a253275 (MD5) Previous issue date: 2007 / CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Este trabalho foi realizado na Unidade de Pesquisa Animal “Senador Álvaro Adolpho” na Embrapa Amazônia Oriental, em Belém, Pará, para avaliar o efeito de quatro níveis de substituição (25%, 50%, 75% e 100%) da leguminosa Pueraria phaseoloides (Roxb.) Benth, no consumo voluntário e digestibilidade aparente da matéria seca (MS), proteína bruta (PB), energia bruta (EB), fibra em detergente neutro (FDN) e fibra em detergente ácido (FDA). O experimento foi de 14 dias de adaptação e sete dias de período experimental, utilizando-se dezesseis ovinos da raça Santa Inês, de dez meses de idade e média de 28 (± 2,44) kg de peso vivo, em delineamento inteiramente casualizado, com quatro tratamentos e quatro repetições. A substituição do quicuio-da-amazônia pela puerária na dieta influenciou o consumo voluntário e a digestibilidade aparente de todos os nutrientes, sendo descrito por função quadrática. Constatou-se um aumento quadrático nos consumos aparentes da FDN e FDA, contudo, com aumento do nível de leguminosa na dieta, foram observadas diminuições significativas nos teor de FDN quando foi fornecido o nível de 75%. A utilização da leguminosa Pueraria phaseoloides, proporciona maior disponibilidade de matéria seca na forragem e elevação do valor nutritivo, principalmente, proteína na dieta, promovendo aumento da produtividade animal. Níveis de substituição de P. phaseoloides, em torno de 100%, possibilitam maior consumo da matéria seca, matéria orgânica, PB e EB, enquanto que, o nível de 75% permite maior consumo das frações fibrosas. / This work was realized in the Animal Research Unit "Senator Alvaro Adolpho" in the Embrapa Eastern Amazon, in Belem, Para State, to evaluate the effect of four levels of substitution (25%, 50%, 75% and 100%) of the Pueraria phaseoloides (Roxb.) Benth leguminous, in the voluntary consumption and apparent digestibility of the dry substance (MS), crude protein (PB), crude energy (EB), detergent neutral fiber (FDN) and acid detergent fiber (FDA). The experiment was of 14 days of adaptation and seven days of experimental period, using sixteen sheep’s, of ten months and 28 (± 2,44) average of kg, in a completely randomized experimental design, with four treatments and four repetitions. The data had been analyzed by software SAS. The substitution of the quicuio-da-Amazônia for the pueraria in the diet influenced the voluntary consumption and the apparent digestibility of all the nutrients, being described for quadratic function. A quadratic increase in the apparent consumptions of the FDN was evidenced and FDA, however, with increase of the level of leguminous in the diet, had been observed significant reductions in the FDN text when the 75% level was supplied. The use of the Pueraria phaseoloides leguminous, provides to greater availability of dry substance in the fodder plant and rise of the nutritional value, mainly, protein in the diet, promoting increase of the animal productivity. Levels of substitution of P. Phaseoloides, around 100%, make possible greater consumption of the dry substance, organic substance, PB and EB, while that, the 75% level allows to greater consumption of the fibroses fractions.

Wong, Wai Yin. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2007. / Includes bibliographical references (leaves 92-108). / Abstracts in English and Chinese. / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Atherosclerosis --- p.1 / Chapter 1.1.1 --- Pathogenesis of Atherosclerosis --- p.2 / Chapter 1.1.2 --- Atherosclerosis and Cardiovascular Disease --- p.4 / Chapter 1.2 --- Cardiovascular Disease (CVD) --- p.5 / Chapter 1.2.1 --- Term Definition --- p.5 / Chapter 1.2.2 --- Risk Factors --- p.6 / Chapter 1.2.3 --- Current Western Medications --- p.7 / Chapter 1.3 --- Reactive Oxygen Species (ROS) --- p.8 / Chapter 1.3.1 --- Impact of ROS --- p.8 / Chapter 1.3.2 --- "Superoxide Anion Radical, Hydrogen Peroxide, Hydroxyl Radical, Nitric Oxide" --- p.9 / Chapter 1.3.3 --- ROS Production by NAD(P)H Oxidases --- p.11 / Chapter 1.3.4 --- ROS Production by Mitochondria --- p.12 / Chapter 1.3.5 --- Lipid Peroxidation --- p.13 / Chapter 1.3.6 --- Other Sources of ROS --- p.15 / Chapter 1.4 --- Antioxidants --- p.16 / Chapter 1.4.1 --- Superoxide Dismutase (SOD) --- p.16 / Chapter 1.4.2 --- Catalase (CAT) --- p.17 / Chapter 1.4.3 --- Glutathinoe Peroxidase (GPx) --- p.17 / Chapter 1.4.4 --- Glutathione-S-Transferase (GST) --- p.18 / Chapter 1.4.5 --- Vitamin E --- p.18 / Chapter 1.4.6 --- Vitamin C --- p.19 / Chapter 1.5 --- Ageing --- p.19 / Chapter 1.6 --- Antioxidants and CVD --- p.21 / Chapter 1.7 --- Traditional Chinese Medicine (TCM) --- p.22 / Chapter 1.7.1 --- Danshen --- p.23 / Chapter 1.7.2 --- Gegen --- p.25 / Chapter 1.7.3 --- Danshen-Gegen Compound Formula (DG) --- p.26 / Chapter 1.8 --- Aim of Study --- p.27 / Chapter Chapter 2 --- In vitro Foam Cells Formation --- p.29 / Chapter 2.1 --- Materials and Methods --- p.29 / Chapter 2.1.1 --- Materials --- p.29 / Chapter 2.1.2 --- Methods --- p.30 / Chapter 2.1.2.1 --- Herbal Preparation by Hot Water Extraction --- p.30 / Chapter 2.1.2.2 --- Resident Peritoneal Macrophages Preparation --- p.31 / Chapter 2.1.2.3 --- "Colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl Tetrazolium Bromide (MTT) Assay" --- p.31 / Chapter 2.1.2.4 --- DG Effect on in vitro Foam Cells Formation --- p.32 / Chapter 2.2 --- Results and Discussion --- p.32 / Chapter 2.3 --- Summary --- p.39 / Chapter Chapter 3 --- In vivo Antioxidant Level --- p.40 / Chapter 3.1 --- DG Effect on in vivo Antioxidant Levels on Young-adult Wistar Rats --- p.40 / Chapter 3.1.1 --- Materials and Methods --- p.40 / Chapter 3.1.1.1 --- Herbal Preparation by Hot Water Extraction --- p.40 / Chapter 3.1.1.2 --- Assay Kits --- p.41 / Chapter 3.1.1.3 --- Antibodies for Protein Expression Determination in Organs --- p.41 / Chapter 3.1.1.4 --- Animals and Experimental Design --- p.41 / Chapter 3.1.1.5 --- Plasma Antioxidants --- p.42 / Chapter 3.1.1.6 --- Lipid Peroxidation and Protein Expression in Organs --- p.46 / Chapter 3.1.1.7 --- Statistics --- p.52 / Chapter 3.1.2 --- Results and Discussion --- p.53 / Chapter 3.2 --- DG Effect on in vivo Antioxidant Levels on Middle-aged Wistar Rats --- p.74 / Chapter 3.2.1 --- Materials and Methods --- p.75 / Chapter 3.2.2 --- Results and Discussion --- p.75 / Chapter 3.3 --- Summary --- p.87 / Chapter Chapter 4 --- Conclusion and Future Work --- p.90 / Chapter 4.1 --- Conclusion --- p.90 / Chapter 4.2 --- Future work --- p.90 / Reference --- p.92 / Related Publication --- p.109

牛樟芝 (Antrodia camphorata; AC) 為台灣特有的藥用真菌,是常見的傳統 中藥,富含三萜類化合物及多醣體。本研究使用牛樟芝、葛花、枳椇子與山楂共 四種萃取物製成之牛樟芝複方飲品 (A. camphorate drink, ACD)為原料,評估此一 複方飲品之抗疲勞功效。本研究從運動表現、疲勞生化指標、肌肉損傷生化指數 以及能量儲存等做為抗疲勞評估標準。將 5 週齡雄性 ICR (Institute of Cancer Research) 小鼠,分為三組,每組各 10 隻,分組如下:(1) 對照組 (vehicle, V); (2) 餵食 15.625 mL/kg 牛樟芝複方飲品 (ACD-1X);(3) 餵食 31.25 mL/kg 牛樟芝 複方飲品 (ACD-2X),連續經口餵食 28 天後進行以下試驗: 1. 測試小鼠前肢抓 力、2. 測試 5%負重游泳力竭時間、3. 進行游泳運動 15 分鐘後立即進行採血觀 察乳酸、血氨、血糖及肌酸激酶、4. 小鼠犧牲後,採集肝臟及肌肉組織進行肝醣 分析與切片。結果顯示,ACD-1X 組 (1124 g) 與 ACD-2X 組 (125 6 g) 在前肢 抓力與對照組 (95 6 g) 相比,分別顯著提升 1.19 與 1.32 倍 (p < 0.05)。ACD-1X 組 (56.714.3 min) 與 ACD-2X 組 (60.717.7 min) 在游泳力竭時間與對照組 (18.23.7 min) 比較分別延長 3.12 及 3.34 倍,均有顯著差異 (p < 0.05)。游泳運 動結束後,ACD-1X 組與 ACD-2X 組之乳酸、血氨及肌酸肌酶均顯著低於對照組 (p < 0.05),而血糖方面則顯著高於對照組(p < 0.05)。肌肉肝醣部分,ACD-1X 組 (1.79 ± 0.12 mg/g) 與 ACD-2X 組 (2.36 ± 0.21 mg/g) 相較於對照組 (1.54 ± 0.06 mg/g),2倍牛樟芝複方飲品組顯著提升1.53倍。肝臟肝醣部分,ACD-1X組 (48.9± 4.1 mg/g) 與 ACD-2X 組 (54.1 ± 5.8 mg/g) 與對照組 (27.0 ± 2.4 mg/g) 比較分別 增加 1.18 及 2.00 倍。研究結果顯示,牛樟芝複方飲品具有增加肝臟及肌肉組織 中的肝醣儲存量,且在游泳運動過程中降低血乳酸、血氨及肌酸激酶的產生。進 一步延長負重游泳力竭的時間及前肢抓力表現。因此,本研究證實牛樟芝複方飲 品具有抗疲勞及提升運動表現之功效。 / Antrodia camphorata is an endemic medical mushroom in Taiwan and has been reported to have multi-biological activities such as anti-fatigue, liver protection, anti-cancer, and anti-inflammation. This study was designed to ascertain the ergogenic and anti-fatigue properties of an A. camphorata drink (ACD) (comprising A. camphorata, Pueraria flos flowers, Hovenia dulcis Thunb, and Crataegus pinnatifida) by forelimb grip strength, load-weighted swimming test, and biochemical examinations in mouse model. Thirty male ICR mice (5-week-old) were randomly divided into three groups (n=10 per group): (1) vehicle; (2) a recommended dosage of 15 mg per 75 mL once per day for adult human (ACD-1X); (3) a 2-fold of recommended usage for adult human (ACD-2X). Mice were given orally either vehicle or A. camphorate drink by gavage for 28 days. The groups ACD-1X and ACD-2X showed significant increases in forelimb grip strength, swimming time to exhaustion skeletal, and muscle glycogen as compared to the vehicle group. Blood lactate and ammonia levels in the ACD-1X and ACD-2X groups were significantly lower than in the vehicle group post a swimming test. Moreover, the activity of plasma creatine kinase (CK), a marker of muscular damage, was significantly decreased in the ACD-1X and ACD-2X groups than vehicle group after swimming exercise. These results suggest that A. camphoratahas great potential for application in relevant fields for its ergogenic and anti-fatigue activities. / 第一章 緒論 .........................................................................................................................1 第一節 研究目的..............................................................................................................2 第二章 文獻探討 .................................................................................................................3 第一節 牛樟芝成份及相關研究......................................................................................3 第二節 葛花成份及相關研究..........................................................................................6 第三節 枳椇子成份及相關研究......................................................................................7 第四節 山楂成份及相關研究..........................................................................................8 第五節 運動與疲勞..........................................................................................................9 第三章 材料與方法 ...........................................................................................................12 第一節 實驗測試樣品....................................................................................................12 第二節 實驗動物之飼養與實驗流程............................................................................14 第三節 血液與組織樣本之收集與前處理....................................................................16 第四節 統計分析............................................................................................................17 第四章 結果與討論 ...........................................................................................................18 第一節 補充牛樟芝複方飲品對於小鼠游泳運動能力表現之測試............................18 第二節 補充牛樟芝複方飲品對於小鼠游泳運動後血液中乳酸、血氨、血糖濃 度以及 CK 活性之分析..................................................................................................19 第三節 補充牛樟芝複方飲品對於小鼠前肢抓力表現之測試....................................20 第四節 補充牛樟芝複方飲品對於小鼠肌肉與肝臟部位肝醣含量之分析................21 第五章 結論 .......................................................................................................................22 參考文獻.............................................................................................................31

Submitted by Alison Vanceto (alison-vanceto@hotmail.com) on 2017-01-23T10:47:55Z No. of bitstreams: 1 DissACCA.pdf: 1129010 bytes, checksum: b80861cac806c1b3baecbc65e887e809 (MD5) / Approved for entry into archive by Camila Passos (camilapassos@ufscar.br) on 2017-01-23T15:47:26Z (GMT) No. of bitstreams: 1 DissACCA.pdf: 1129010 bytes, checksum: b80861cac806c1b3baecbc65e887e809 (MD5) / Approved for entry into archive by Camila Passos (camilapassos@ufscar.br) on 2017-01-23T15:47:35Z (GMT) No. of bitstreams: 1 DissACCA.pdf: 1129010 bytes, checksum: b80861cac806c1b3baecbc65e887e809 (MD5) / Made available in DSpace on 2017-01-23T15:47:48Z (GMT). No. of bitstreams: 1 DissACCA.pdf: 1129010 bytes, checksum: b80861cac806c1b3baecbc65e887e809 (MD5) Previous issue date: 2016-06-07 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Maize can be intercropped with green manures, which in addition to performing the coverage and soil protection, the weeds control, also cycle nutrients and perform nitrogen biological fixation. This study aimed at evaluating the growth, yield of grains and straw, the content of macronutrients of maize and the weeds control in intercropping with perennial green manures sown at two different maize growth stages. The experiment was conducted in the agricultural year of 2014/2015, at UFSCar/Araras-SP, in randomized complete blocks in factorial scheme 3 x 2 + 1 with 4 repetitions. The factors were: 3 species of green manures (calopogonio (Calopogonium mucunoides Desv.); kudzu (Pueraria phaseoloides (Roxb) Benth.) and perennial soybean (Neonotonia wightii (Wight & Arn) Lackey.) and two green manures sowing times (sowing at the V4 - four expanded leaves and VT – bolting of maize). And a control treatment, maize monoculture. The evaluations were: in the R1/R2 of maize stage - milky/doughy grain (height (ALT); diameter (DIA); specific leaf area (AFE); leaf area index (IAF); stalk dry matter (MSC), leaves (MSF) and total (MST) of maize; Falker chlorophyll index (ICF); number of leaves above the upper ear (FAS)); in the R5 of maize stage – chalky grain (dry mass of green manure (MSA); total dry mass maize intercropping/green manure (MSTC); percentage of land cover (PC) and dry mass of weeds (MSE)); in the R6 – maize harvest (final plant stand (EST); prolificacy (PROL); number of rows per ear (NFE); number of kernels per row (NGF); yield of grain (PROD); weight of 100 grains (P100) and content of macronutrients of maize). It was found higher production of EF with the sowing of green manures in the V4 stage (69,814 plants ha-1), being the intercropping maize/calopogonio with higher values of MSA and PC, with 1002.00 kg ha-1 and 35.63%, respectively. The highest P100 occurred in the VT (32.89 g). There was not difference for the other variables. The PROD was 9.04 t ha-1 and 7.0 t ha-1 of MSTC. The smallest leaf N content was observed in the intercropping with calopogonio in V4 (22.75 g kg-1). The Ca had smallest levels for the perennial soybean in the VT and kudzu in the V4. The smallest Mg content was in intercropping with the soybean perennial in the VT, in the P was in the intercropping with calopogonio and S, when the sowing of the green manures occurred in V4 of maize. For K, there was not statistical difference. The MSTC production was not affected by green manures, with maize straw production in monoculture and in the intercropping systems exceeding the minimum amount recommended for ground cover (6.0 t ha-1). The green manures negatively affect the content of P, K, Ca, Mg and S in maize and calopogonio sown in the VT, the kudzu and the soybean perennial sowing in the V4 and VT, contributed to provide N to maize. / O milho pode ser consorciado com adubos verdes perenes, que além de realizarem a cobertura e proteção do solo, o controle de espontâneas, ciclam nutrientes e realizam a fixação biológica de nitrogênio. Objetivou-se com este trabalho avaliar o crescimento, a produtividade de grãos e de palhada, o teor de macronutrientes do milho e a supressão das plantas espontâneas em cultivo consorciado com adubos verdes perenes semeados em duas épocas distintas de desenvolvimento do milho. O experimento foi realizado na safra de 2014/2015, na UFSCar/Araras-SP, em blocos casualizados no esquema fatorial 3 x 2 + 1 com 4 repetições. Os fatores foram: 3 espécies de adubos verdes perenes (calopogônio (Calopogonium mucunoides Desv.); puerária (Pueraria phaseoloides (Roxb) Benth.) e soja-perene (Neonotonia wightii (Wight & Arn.) Lackey) e 2 épocas de semeadura (semeadura no V4 – quatro folhas expandidas e VT – pendoamento do milho). E a testemunha, o milho solteiro. Avaliaram-se: no estádio R1/R2 do milho - grão leitoso (altura (ALT), diâmetro (DIA), área foliar específica (AFE), índice de área foliar (IAF), massa de matéria seca do colmo (MSC), folhas (MSF) e total (MST) do milho, índice de clorofila Falker (ICF) e nº de folhas acima da espiga superior (FAE)); no estádio R5 do milho – grão farináceo (massa de matéria seca dos adubos verdes (MSA), massa de matéria seca total do consórcio milho/adubos verdes (MSTC), porcentagem de cobertura do solo (PC) e massa de matéria seca das plantas espontâneas (MSE)); no estádio R6 – colheita do milho (estande final de plantas de milho (EF), prolificidade (PROL), número de fileiras por espiga (NFE), número de grãos por fileira (NGF), produtividade de grãos (PROD), peso médio de 100 grãos (P100) e o teor de macronutrientes no milho). Foi encontrado maior EF com a semeadura dos adubos verdes no V4 (69.814 plantas ha-1), sendo o consórcio milho/calopogônio com maiores valores de MSA e PC, com 1002,0 kg ha-1 e 35,63%, respectivamente. O maior P100 ocorreu no VT (32,89 g). Não houve diferença para as outras variáveis. A PROD foi de 9,04 t ha-1 e 7,0 t ha-1 de MSTC. O menor teor de N foliar do milho foi observado no consórcio com calopogônio no V4 (22,75 g kg-1). O Ca teve menores teores para a soja perene no VT e a puerária no V4. O menor teor de Mg foi no consórcio com a soja perene semeada no VT, de P foi no consórcio com calopogônio e de S, ocorreu quando a semeadura dos adubos verdes ocorreu no V4 do milho. Para o K, não houve diferença estatística. A produção de MSTC não foi influenciada pelos adubos verdes, com a produção de palha do milho em cultivo solteiro e nos sistemas consorciados superior a quantidade mínima recomendada para cobertura do solo (6,0 t ha-1). Os adubos verdes afetaram de forma negativa o teores foliares de P, K, Ca, Mg e S do milho e o calopogônio semeado no VT, a puerária e a soja perene semeados no V4 e VT, contribuíram para o incremento de N ao milho.

Tam Wing Yin. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. / Includes bibliographical references (leaves 82-100). / Abstracts in English and Chinese. / English abstrac --- p.I / 中文摘要 --- p.VI / Glossary --- p.X / Chapter Chapter 1. --- Background: / Chapter 1.1. --- Coronary heart disease in Hong Kong --- p.1 / Chapter 1.2. --- Coronary heart disease and atherosclerosis --- p.2 / Chapter 1.3. --- Pathogenesis of atherosclerosis --- p.2 / Chapter 1.4. --- Risk factors for atherosclerosis --- p.5 / Chapter 1.5. --- Homocysteine --- p.6 / Chapter 1.6. --- Folate --- p.10 / Chapter 1.7. --- Vitamin B12 --- p.13 / Chapter 1.8. --- Adhesion Molecules --- p.14 / Chapter 1.9. --- Phytoestrogen --- p.17 / Chapter 1.10. --- Secondary prevention of coronary artery disease --- p.20 / Chapter Chapter 2. --- "Heart disease, Danshen and Gegen in Chinese medicine" / Chapter 2.1. --- The record of Cardiac symptoms in Chinese Medicine --- p.24 / Chapter 2.2. --- Danshen (Salvia Miltriorrhiza) --- p.25 / Chapter 2.3. --- Gegen (Radix Pueraria) --- p.28 / Chapter Chapter 3. --- Surrogate atherosclerotic markers / Chapter 3.1. --- Flow-mediated dilatation of brachial artery (FMD) --- p.31 / Chapter 3.2. --- Carotid intima media thickness (IMT) --- p.32 / Chapter Chapter 4. --- Method / Chapter 4.1. --- Rational of the study --- p.33 / Chapter 4.2. --- Clinical protocol --- p.35 / Chapter 4.3. --- Measurement of plasma homocysteine --- p.38 / Chapter 4.4. --- Measurement of folate and vitamin B12 --- p.40 / Chapter 4.5. --- Measurement of soluble cellular adhesion molecules (CAMs) --- p.41 / Chapter 4.6. --- Measurement of plasma enterolactone --- p.43 / Chapter 4.7. --- Measurement of plasma hs-C-reactive protein --- p.44 / Chapter 4.8. --- Other laboratory tests --- p.45 / Chapter 4.9. --- High resolution ultrasound imaging --- p.46 / Chapter 4.10. --- Statistical analysis --- p.49 / Chapter 4.11. --- My contribution to this joint project --- p.49 / Chapter Chapter 5. --- Results / Chapter 5.1. --- Recruitment and outcomes of subjects --- p.51 / Chapter 5.2. --- Baseline characteristics --- p.53 / Chapter 5.3. --- Medical history and treatment received in the study subjects --- p.54 / Chapter 5.4. --- Safety profiles --- p.55 / Chapter 5.5. --- Severe adverse events --- p.56 / Chapter 5.6. --- Lipid profiles --- p.57 / Chapter 5.7. --- Secondary endpoints --- p.58 / Chapter 5.8. --- Primary endopoints --- p.59 / Chapter 5.9. --- The effect of statin usage on the primary endpoints / Chapter 5.10. --- The major determinant of the change in FMD by multivariate logistic regression / Chapter 5.11. --- Progress of lipid profiles and primary endpoints in the open label phase / Chapter Chapter 6. --- Discussion / Chapter 6.1. --- Brachial FMD --- p.66 / Chapter 6.2. --- Carotid IMT --- p.69 / Chapter 6.3. --- Brachial GTN --- p.70 / Chapter 6.4. --- Lipid-lowering effect --- p.72 / Chapter 6.5. --- Phytoestrogen --- p.72 / Chapter 6.6. --- Folate --- p.73 / Chapter 6.7. --- Vitamin B12 and glucose --- p.76 / Chapter 6.8. --- Summary of possible anti-atherogenic mechanism of D&G --- p.76 / Chapter 6.9. --- Placebo effect --- p.77 / Chapter 6.10. --- Safety profile --- p.77 / Chapter 6.11. --- Limitation of the study and suggestion of solution --- p.77 / Chapter 6.12. --- Suggestions and ummary of the future work --- p.79 / Chapter Chapter 7. --- Conclusions --- p.81 / References --- p.82