With biodiversity loss and ecosystem degradation ongoing, the conservationists' toolkit needs to be augmented by innovative and sometimes bold solutions. It is already apparent that the scale of the problem exceeds the capabilities of any one organisation working in isolation. However, collaboration between stakeholder groups may have the potential to enhance conservation outcomes. Cross-sector collaborations, such as those between non-governmental organisations (NGOs) and corporations, are proliferating in conservation. However, little is known about their efficacy and subsequent impact on the wider natural environment, with many assumptions based on anecdotal rather than empirical evidence. This thesis aims to fulfil an important and substantial knowledge gap by using data-driven approaches to understand partnerships in conservation from the perspective of conservation NGOs. Firstly, I assess the types and prevalence of partnerships between conservation NGOs and corporations and find a diverse range of projects are occurring. For example, financial donations are a frequent form of NGO-corporate interaction, but other collaborative activities such as terrestrial ecosystem restoration and educational activities, are regularly reported. Secondly, I evaluate an NGO-led collaborative network to understand what motivates organisations to initiate a membership committing them to voluntary environmental measures that go beyond regulatory compliance. Results suggest that the majority of members join to fulfil strategic objectives such as reputation protection, rather than for altruistic reasons. Furthermore, participants with stronger sustainability credentials occupy the most influential positions within the network, meaning that they are strongly positioned to receive and disseminate information. Lastly, I use social network analysis to explore how a conservation NGO delivers its landscape-scale conservation projects by acquiring key resources from multiple partners. Results indicate that partners providing land-based support are well connected with one another, meaning that landscape-scale conservation activities can be coordinated more easily. Furthermore, the in-kind support network, primarily comprising NGOs, displays the greatest innovative capacity. The research presented in this thesis highlights that cross-sector partnerships have a central role to play in bridging the interests of different stakeholder groups, and reflect the inclusive vision of conservation we should all be striving to create.

Global biodiversity is being lost at an unprecedented rate, such that we have entered the sixth mass extinction in the history of the earth with emerging infectious diseases (EID) recognised as an important contributor to this loss. Amphibian chytridiomycosis is an EID that has driven very rapid declines in, or even extinctions of, hundreds of amphibian species. Infectious diseases such as chytridiomycosis, caused by the fungal pathogen Batrachochytrium dendrobatidis (Bd), often persist in biological and non-biological reservoirs making them difficult to eradicate. In turn, this makes reintroductions of target species challenging due to the risk of infection. This thesis investigates the critically endangered mountain chicken (Leptodactylus fallax) as a case study of the population impacts of a chytridiomycosis epidemic and to test the effectiveness of strategies to mitigate the effects of the disease. Specifically, this research (1) charts the decline of the mountain chicken on the only two islands on which it exists, and determines the impact on genetic diversity; (2) tests whether anti-fungal treatment can improve the survival of mountain chickens with Bd infection in the wild; (3) examines the role of Bd reservoir species in causing Bd infections of reintroduced mountain chickens; and (4) determines habitat features that are predictors of infection at release sites. Chytridiomycosis drove the mountain chicken to near extinction on Dominica in 2002 and Montserrat in 2009, in one of the fastest recorded vertebrate species declines, leading to a significant loss of genetic diversity. On Montserrat, treating mountain chickens with an anti-fungal drug (itraconazole) during the chytridiomycosis epidemic improved survival rates and reduced Bd infection rates in the short term, but did not provide long-term protection. Although mountain chickens have been driven to near-extinction by Bd infection on Montserrat, the pathogen persists in two sympatric reservoir species which are not impacted by Bd infection, the most prolific of which (Eleutherodactylus johnstonei) displays strong seasonality in Bd infection prevalence and load. Timing mountain chicken reintroduction to occur during the period when tree frog Bd infection was at its lowest was tested to determine the impact on reintroduction success. Multi-state mark-recapture modelling applied post-release showed that optimising the timing of release reduced Bd infection rates and increased survival. Radio-tracking was utilised with geographic profiling to determine that release site water bodies were likely sources of Bd infection in reintroduced mountain chickens. This could inform targeted mitigation of the pathogen and improve future reintroduction success. Where species have been extirpated in the wild, and an irreversible threat such as an EID persists, novel reintroduction strategies are required. These include optimising the timing and conditions of release in order to minimise the impact of the threat along with targeted mitigation measures such as individual level treatments.

This thesis addresses six main objectives answering questions on the origin, nature and social organization of SNS and their custodians; motivations for, and BCD conservation consequences of, their maintenance; threats SNS and ancestral institutions face and existing governance and protection instruments, with focus on local perceptions among the Wonsho of Sidama, Ethiopia. The study employs anthropologically-oriented, but interdisciplinary, conceptual framework and mixed methods to collect and analyse data. A year of fieldwork (July 2012-June 2013) was carried out using six major data collection methods (including interviews, BD inventory and HHS). The data were analysed using NVivo 10 and SPSS 20/21. The results are presented and discussed in seven key thematic areas and six chapter headings. The main findings are summarized as follows: 1. Forty-eight SNS (whose sizes ranging from a site of a single tree to a 90.6 ha and ages from 28 to ca 375 years) were identified in seven PAs. Three criteria were used to identify a typology of Wonsho SNS: spatial-clan structure, function and protection status. SAR was identified as the core of the origin, social organization, governance and geography of SNS and other BCD protection areas. Twenty-two of SNS were protected by SAR practitioners and four by Protestant Christians. The rest were either lost or transformed. 2. Answers to the question of why SNS are maintained are interpreted as linked to ancestral conceptions of the natural world, knowledge about, and practice relating to, it. The people valued SNS and native trees as ‘life’, ‘beauty’, ‘ancestor symbolizers’, ‘temples’, ‘wealth’, ‘shade’, ‘healing agents’, ‘food banks’, ‘place and name identifiers’, and ‘tribunal courts’ among others. Certain salient norms and practices, supporting tree biodiversity, are identified and interpreted as the foundation of the motivation for the maintenance of SNS. 3. 154 floral and 33 faunal species were documented for their reported and observed past and present existence in 26 of the 48 SNS and other informal protection areas. A partial inventory identified about 133 flora and some fauna, including two locally endangered species, Colobus guerezza and Tauraco ruspoli in various SNSs. Twenty-two locally reported endangered native trees were found here, of which ten were reportedly found nowhere. Eighteen major woody species were identified as extractively conserved in various informal protection areas, notably agroforests. 4. Forty-three types of uses of trees were identified. Eighteen woody species were identified as playing crucial socio-economic role; seven of these being culturally important and Podocarpus falcatus was identified as a truly ‘cultural keystone species’. The maintenance of SNS and native trees has important role through provisioning, regulating, supporting and cultural ecosystem services. 5. Maintenance of SNS and other botanic landscapes were found to contribute positively towards community health, herbal medicine and conservation of medicinal plants. SNS are perceived as key resources for health and wellbeing. Sixty-two percent of surveyed HHs accessed medicinal plants from SNS where 48% of the identified plants (including nine that were reported as locally endangered) were found. 6. The SNS and ancestral institutions faced threats. Fourteen SNS were lost, eight severely degraded through other land uses and the existing 26 also threatened in seven studied PAs. Twenty-two important native trees were reportedly threatened; ten of these exited only in the SNS. Twelve native woody species were reportedly lost. SAR is threatened (e.g. declined from 13.6% in 1994 to 2.7% in 2007). Eroding factors, especially external ones, have been intensifying since the 1890s, but momentum added over the past 50-60 years, salient drivers being introduction of cash economy, modern religions, modern education, misguided state policies, rapid population growth and resultant socio-economic pressures. 7. The SNS have for centuries been protected through ‘spirit agency and policing’ in a structure that gave supreme place to ancestors who influenced and guided governance. Some key principles of SNS management were identified, including ‘spirit-policing’, dreams and oracles in decision making, protecting entire habitat, protecting species, etc. In recent years, protection efforts have improved, with emerging collaborative governance, but these suffered from poor resourcing, coordination and fragmentation; and the future of SNS, native tree species and the SAR seemed uncertain despite some locals were optimistic. The study concludes the SNS and associated institutions of Wonsho have resiliently existed as ‘guardians of Sidama biocultural diversity’ and are showcases for the mutual adaptations of tree biodiversity and ancestral traditions. The study discusses a set of implications and recommendations for further research and action. The contribution of the study lies in the following areas which appear to be under-represented in the current literature: (a) qualitative analysis of the ontology, nature, structures, functions, geography and dynamisms of SNS and custodians, demonstrating that Wonsho SNS are not relics from static past but dynamic socio-ecological systems; (b) in-depth discussion of the role of SNS in conserving both biodiversity and cultural diversity; (c) a nuanced analysis of why and how the SNS are maintained, (d) local perceptions and parameters of the values and roles of, and threats facing, SNS and related local institutions; (e) our understanding of what constitutes ‘biocultural diversity’ and the indicators for cultural diversity when this concept is applied at a local scale; (e) interdisciplinary conceptual and analytical tools to understand the socio-ecological and biocultural systems embodied in sacred sites, combining concepts from a range of social and natural sciences, notably anthropology and conservation biology.

This dissertation presents the first evaluation of a VCA in terms of its multi-scalar governance approach with reference to the principles of the ICCAs category and the CPR principles for institutional arrangements for sustainable natural resource management. The research techniques applied to develop this research included: (1) document revision on national legislation for protected establishment and management; (2) forty four semi-structured interviews with conservation practitioners at different administrative levels, as well as (3) direct observations, 32 semi-structured and unstructured interviews to conform an in-depth case study of the VCA of El Reten, in San Miguel Chimalapa, Oaxaca, Mexico. Devolution processes in El Reten were analysed in terms of the bundles of rights or powers that local community holds for natural resource management after the certification of El Reten and during its early implementation. The issues examined by these dissertation have explored for the first time who is entitled to “give” which powers back to local communities; the actual procedures that allow these approaches to be called community-driven when ICCAs can only retain “power” by conforming to externally defined criteria, and finally, if devolution is happening, the way “bundles of rights” - or powers - (Ribot and Peluso 2003) interact with external criteria for conservation. The case study of El Reten provides clear examples of the implications of the formalisation of a VCA over local governance structures. These decentralised approaches for conservation are also subject to elite capture and the trade-offs between the availability of economic resources and local autonomy, as well as between administrative efficiency and equity and legitimacy. The VCA in El Reten represents the ideal scenario for the tragedy of the commons (Hardin 1968), where the establishment of a VCA, the arrival of economic incentives and the overlooking of the local political context by conservation agencies is fuelling the tragedy instead of alleviating it. This dissertation shows this explicitly in the context of the newly developed VCA category in Mexico for the first time.

Amphibians are the most threatened class of vertebrate, and rates of species decline and extinction far exceed those seen historically. Habitat loss, climate change, over-exploitation and emerging infectious disease have all been identified as threatening processes. The trade in amphibians has been implicated in over-exploitation through the harvesting of wild animals, and as an important pathway for the global spread of the fungal pathogen, Batrachochytrium dendrobatidis (Bd). However, there are no analyses of how Bd may spread through the trade chain. This thesis addresses this issue by (1) determining the prevalence of Bd at different stages of the trade chain; (2) examining knowledge, husbandry protocols and biosecurity among retailers and (3) assessing the risk of Bd dissemination into the wild in the UK. Approximately 20,000 amphibians from at least 11 countries enter the UK annually via Heathrow Animal Reception Centre. Overall Bd prevalence was 3.6%, but was confined to six of the 43 genera encountered, and only detected in shipments from the USA and Tanzania. Amphibians were sold by 30% of the estimated 3500 livestock retailers in the UK, but made a low contribution to overall income. Disease awareness and knowledge in retailers was found to be lacking, although husbandry standards were deemed to be appropriate. Mortality appeared to be influenced by restocking methods and number of species held, but mass die-offs as a result of disease were generally uncommon. Screening of over 2000 amphibians from 148 retailers for Bd revealed a prevalence of 5.8%, but the geographic distribution of infection in the UK was patchy, and was more prominent in aquatic species. A risk assessment conducted according to the framework set out by the World Organisation of Animal Health, identified regions and sections of the trade that pose the greatest threats in terms of introducing Bd, and assessed various mitigation measures. The consequences of novel strains of Bd and a second, recently discovered Batrachochytrium species were found to pose a risk to both native UK and captive amphibians. As trade bans are unlikely to be feasible or effective, alternative measures to mitigate the impact of disease are evaluated.

The creation and management of protected areas is our principal approach to conserving biodiversity worldwide. Management and governance models for these diverse institutions have become more pluralistic in recent decades, moving away from the traditional exclusionary protected area model that has proliferated historically. Indeed, most new protected areas are being established for ‘multiple-use’ and, therefore, permit a range of human livelihood activities to occur within their boundaries. However, we know little about how such sites can be effectively managed. In this thesis, I use an interdisciplinary mixed-methods approach to investigate the implementation of new multiple-use protected areas in Madagascar. Madagascar is a global conservation priority characterised by high levels of endemism, and has a largely forest-dependent biota. Since most of the human population is rural and dependent on natural resources for subsistence and income to differing extents, the expanded protected area system is managed for both conservation and socioeconomic goals (poverty alleviation and development). However, these objectives may be conflicting since human resource use can be a significant driver of biodiversity loss. I begin by examining trends in new protected area establishment at the nationwide-level to generate insights into protected area categorisation, and the role of natural resources and protected areas in poverty alleviation. I then consider the impacts of forest use on biodiversity, through a literature review and empirical study of bird and reptile communities across a degradation gradient. The findings indicate that habitat change arising from forest use may impact the high-value, endemic component of the fauna most negatively. In addition, I develop a simple index to enumerate the conservation value of different species. This is then used to determine how degradation influences the conservation value of exploited habitats, as well as assessing if the index is a suitable tool that can be used to prioritise conservation investment across a portfolio of sites. Finally, I seek to understand the drivers of natural resource use by rural communities within the Ranobe PK32 protected area, and discover that both bushmeat hunting and charcoal production are fallback activities or supplements to other livelihoods. The evidence collated in the thesis, derived from both ecological and social perspectives, suggests that managing new protected areas in Madagascar for conservation and development is overambitious, and that, at least in forest areas, management cannot be optimised towards both goals simultaneously.

The Mijikenda sacred natural sites (SNS) contribute towards Kenya’s East African Coastal Forest ecosystem. This ecosystem is highly biodiverse and important to the conservation of many rare and endemic species. The SNS are therefore thought to be very important to biodiversity locally and globally, as well as playing a significant role in the preservation of the local traditional culture. Whilst it is known that the SNS contain coastal forest, there are no accurate estimates on the amount, nor are there assessments of habitat diversity within the sites and no systematic surveys have been done in the past 20 years. In addition, degradation of the sites has been described, but the level of encroachment and amount of forest loss has not been measured. A major driver of the degradation and deforestation of SNS is thought to be cultural change, leading to a decrease in the adherence to traditional practices, and loss of knowledge and respect for local customs. The existing management of the SNS is based on the traditional laws associated with the SNS; enforcement is left to local Kaya Elders, and it follows the assumption that the Mijikenda are a homogenous and culturally static group. As such it is thought that changes in culture and values systems may be undermining the successful conservation of the sites. However, again, whilst changes within the local communities surrounding the SNS have been described, no research has been done on how such changes may have altered the attitudes and values of the local people in this region, or what impact this may have on the preservation of the SNS. The aims of this thesis are to: measure the amount of costal forest within the Mijikenda SNS; to assess if the habitat heterogeneity within the sites; investigate their potential for biodiversity; measure the amount of forest loss within the sites, and the amount of encroachment that they suffer from; understand the current attitudes and values of the local communities towards the SNS, their culture, and conservation; compare current attitudes and values to what would be expected traditionally; investigate the use of resources from the Kayas by the local communities; and, in light of these questions, assess the efficacy of the existing management plan in light of the current landscape in which the SNS are located and any changes in local culture, and associated values. The results show that the Mijikenda SNS contain a substantial proportion (1.4%) of Kenya’s coastal forest. Due to their habitat heterogeneity and habitat features, as well as being some of the only forest habitat within a degraded landscape, they are important to both local and global conservation, including the possibility to maintain viable populations of rare and endemic species. Whilst the rate of forest loss within the SNS was found to be significantly lower than forest loss outside the sites, almost all sites were undergoing encroachment, degradation and forest loss. The local communities were found to be diverse, with different demographics, attitudes, values and behaviours. There has been a significant departure from the traditional culture, including a decrease of participation in traditional practices, a lack of adherence to customary laws, and a loss of traditional knowledge. The SNS were found to be important for resources to the local communities; however, extraction is not being monitored or managed for. In addition, ongoing developments in the region could pose a significant threat to the SNS. This research provides the first set of accurate estimations of coastal forest within the sites, and the range of habitat heterogeneity and potential contribution to biodiversity they make. It also offers the first set of accurate measurements, of the extent of encroachment, and forest loss, that a number of sites on the north coast have undergone. In addition it provides some of the only large-scale social data associated with the Mijikenda SNS. This thesis shows that whilst some of the threats to the sites, and changes in local culture have been observed in the past, none have been accounted for in the current management plans associated with the sites. The management of the SNS needs to be redesigned to account for the changes within the local communities and the surrounding area, as well as addressing the threats that the SNS face. Management should be created on a site-by-site basis, to work with all stakeholders in the area, and must encompass the changes which are happening within the region. In addition, interventions to address conflicts within communities, provide alternative access to resources, and to improve transmission of knowledge, need to be put in place to aid communities in protecting the SNS. Management of the sites must be done jointly by the local people, the government, and NGOs, with the local communities predominantly having autonomy over the protection of the sites and their culture. This research contributes to the understanding of the roles that SNS play in the conservation of biodiversity; and the issues that arise for the conservation of traditionally managed sites of communities undergoing cultural change. It will help to provide information which can be used to address the management of the Mijikenda SNS as well as SNS and community conserved areas around the world.