Quality control on panoramic radiography units, calculation of effective dose, principles of function of panoramic units, QC protocols, radio-protection issues. / Έλεγχος ποιότητας οδοντιατρικών πανοραμικών συστημάτων ακτινογράφησης, υπολογισμός ενεργού δόσης, αρχές λειτουργίας πανοραμικών συστημάτων, πρωτόκολλα ποιοτικού ελέγχου, ζητήματα ακτινοπροστασίας.

No presente trabalho serão apresentados testes de comissionamento e de avaliação da utilização do portal dosimetry, da Varian, no controle de qualidade dos planejamentos de radioterapia de intensidade modulada. Os testes de comissionamento foram realizados para caracterizar o portal dosimetry em termos dosimétricos e para avaliar a sua possível aplicação em radioterapia. Esses testes demonstraram que o portal dosimetry possui todas as características necessárias para ser utilizado em dosimetria na radioterapia tais como linearidade da resposta com a dose, independência com a taxa de dose, reprodutibilidade, dentre outras. A avaliação da utilização do portal dosimetry no controle de qualidade de IMRT foi realizada em duas etapas: avaliação da capacidade do dispositivo em detectar erros propositalmente introduzidos em fluências simples e em fluências complexas. Foram introduzidos erros de magnitude conhecida em áreas determinadas das fluências e foi realizado o controle de qualidade dessas fluências com o portal dosimetry e com mais três sistemas dosimétricos: câmara de ionização, filme e matriz de câmaras de ionização. Os dados obtidos com o portal foram comparados com os dos outros dispositivos e todos foram capazes de identificar os erros introduzidos de maneira satisfatória, sendo os valores, normalizados para a fluência original, obtidos com o dosímetro portal indênticos aos da câmara de ionização e aos da matriz de câmaras de ionização (seven29) e diferindo em até 2% dos valores obtidos com os filmes. As fluências medidas com o portal dosimetry foram avaliadas em termos quantitativos e qualitativos. Os índices da função gama fornecidos pelo software de análise do portal dosimetry não apresentaram regras definidas de comportamento em relação aos erros introduzidos e por essa razão a análise qualitativa se mostrou indispensável nos casos avaliados. / In this paper we present commissioning testing and evaluation of the use of Varians portal dosimetry in the quality assurance of intensity-modulated radiotherapy. The commissioning tests were performed to characterize the portal dosimetry in terms dosimetric and to assess the its possible application in radiotherapy. These tests demonstrated that portal dosimetry has all the characteristics to be used for dosimetry in radiotherapy such as linear response with dose, the independence of dose rate, reproducibility, and others. The evaluation of the use of portal dosimetry in quality control of IMRT was performed in two steps: assessing the ability of the device to detect errors deliberately introduced in simple and complex fluences. Errors of known magnitude were introduced in certain areas of fluences and was carried out quality control of these fluences with portal dosimetry and three dosimetric systems: ionization chamber, film and array of ionization chambers. The data obtained from the portal were compared with those of other devices and all were able to identify errors introduced satisfactorily, the values, normalized to the original fluence, obtained with the portal dosimetry were similar to the ionization chamber and the array of ion chambers (seven29) and differing in up to 2% of the values obtained with the films. The fluences measured with the portal dosimetry were evaluated both quantitatively and qualitatively. The index of the gamma function provided by software analysis of portal dosimetry showed no defined rules of behavior in relation to the errors introduced and for this reason the qualitative analysis has proved indispensable in cases evaluated.

Made available in DSpace on 2015-03-03T11:52:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-07-11Bitstream added on 2015-03-03T12:06:11Z : No. of bitstreams: 1 000810536_20150711.pdf: 232432 bytes, checksum: 6ee1cf6466125450a3423816485e0d15 (MD5) Bitstreams deleted on 2015-08-03T12:21:13Z: 000810536_20150711.pdf,. Added 1 bitstream(s) on 2015-08-03T12:22:25Z : No. of bitstreams: 1 000810536.pdf: 1780355 bytes, checksum: ba562b46aa8c9d557ca573c7a698fd1f (MD5) / Com a busca por novas fontes de energia renovável, o biodiesel foi introduzido na matriz energética brasileira, e com isso a ANP assumiu a atribuição de regular e fiscalizar a qualidade do biodiesel. Quimicamente, o biodiesel é uma mistura de ésteres metílicos de ácidos graxos, derivados de óleos vegetais e gorduras animais, geralmente produzidos por uma reação de transesterificação, em que a matéria prima reage com um álcool, na presença de um catalisador. É a principal alternativa ao diesel fóssil e as principais vantagens de seu uso é o fato de que é um recurso renovável não tóxico, e que diminui as emissões de gases poluentes quando adicionado ao diesel, tornando-o importante não apenas do ponto de vista energético, mas também ambiental. A ANP estabelece 25 parâmetros que devem ser analisados para certificar a qualidade do biodiesel, e os métodos analíticos que devem ser usados para sua determinação. Dentre esses, o teor total de ésteres pode ser considerado o principal parâmetro, e os métodos oficiais de análise são por cromatografia gasosa convencional e apresentam por volta de 31 minutos de corrida cromatográfica. Este trabalho relata o uso de cromatografia gasosa ultra-rápida para determinar o teor total de ésteres do biodiesel, diminuindo o tempo de análise em 15 vezes (1,5 min). A diferença entre a CG-UR e a CG-C está no menor comprimento da coluna capilar, de 2 a 10m, diâmetro interno reduzido, entre 0,05 e 0,10mm, aquecimento resistivo da coluna, ao invés do uso do forno convencional e taxa elevada de aquecimento da coluna. Através da elaboração de uma curva analítica e posterior validação foi possível quantificar o teor total de ésteres em biodiesel de soja em um curto intervalo de tempo, com a mesma confiabilidade. / With the search for new sources of renewable energy, biodiesel was introduced in the Brazilian energy matrix, and the ANP took the assignment to regulate and supervise the quality of biodiesel . Chemically, Biodiesel is a mixture of a fatty acids methyl esters, derived from vegetable oils and animal fats, usually produced by a transesterification reaction in which the feedstock is reacted with an alcohol in the presence of a catalyst. It is the main alternative to fossil diesel and the main advantages of its use is the fact that it is a non-toxic renewable resource, and reduces greenhouse gas emissions when added to diesel, making it important not only from the point of view of energy but also environmental . The ANP provides 25 parameters that must be analyzed to ensure the quality of biodiesel and analytical methods to be used for its determination. Among these , the total ester content can be considered the main parameter and the official methods of analysis is by conventional gas chromatography and feature around 31 minutes into the chromatographic run . This paper reports the use of ultra -fast gas chromatography (GC- UF) to determine the total ester content of biodiesel , reducing analysis time by 15 times (1.5 min). The difference between the UF with GC- GC- C is the smaller capillary column length of 2 to 10m, reduced internal diameter between 0.05 and 0.10 mm , resistive heating of the column, rather than the use of the oven conventional and high rate of heating of the column. Through the development of an analytic curve and subsequent validation was possible to quantify the total ester content in biodiesel from soybean in a short period of time, with the same reliability.

Orientador: João Bosco Faria / Banca: André Ricardo Alcarde / Banca: Mauricio Boscolo / Banca: Daniela Cardoso Umbelino Cavallini / Banca: Valéria de Carvalho Santos Ebinuma / Resumo: Objetivo: analisar o uso do cobre, aço inoxidável e da prata na produção da aguardente de liquor de laranja e seus efeitos na qualidade química e sensorial da bebida, assim como o potencial de mercado da aguardente, junto às bebidas destiladas envelhecidas encontradas no mercado brasileiro. Métodos: o liquor fermentado pelas leveduras de descarte da indústria cervejeira foi destilado em alambiques de cobre e aço inoxidável. Os destilados foram submetidos ao processo de bidestilação utilizando-se no interior dos capitéis dos alambiques dispositivos de cobre e de prata, sendo assim obtidas sete aguardentes, as quais foram então envelhecidas em ancorotes de carvalho novos, por seis meses. Foram realizadas em todas as aguardentes as determinações de cobre e acidez volátil segundo a Instrução Normativa n° 24; aldeídos, ésteres, metanol, álcoois superiores, acidez volátil e furfural, por cromatografia em fase gasosa com detector de ionização de chama e carbamato de etila por cromatografia gasosa com detector de espectrometria de massas. O potencial de mercado da aguardente de liquor de laranja foi avaliado comparando-se o perfil sensorial da aguardente com bebidas destiladas envelhecidas comercializadas como cachaça, rum, whiskey e tequila. A análise sensorial foi realizada utilizando-se testes descritivo (Perfil Livre) e afetivo (Teste de aceitação e intenção de compra). Com os dados do Perfil Livre foi realizada a Análise Procrustes Generalizada (GPA). Só fize... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Objective: Analyze the use of copper, stainless steel and silver in the production of orange liquor spirit and its effects on the chemical and sensorial quality of the beverage, as well as the market potential of the new spirit along with the aged distilled beverages found in the Brazilian market. Methods: The liquor fermented by brewer's yeast from the brewing industry was distilled in copper and stainless steel stills with a capacity of twenty liters, heated with direct flame. The distillates were then subjected to the double distillation process using copper or silver devices in the interior of the capitals of the stills, thus obtaining seven different beverages. The spirits thus obtained, were aged in new oak barrels with a capacity of five liters for six months. All determinations of copper and volatile acidity were carried out in all spirits according to Normative Ruling n. 24; aldehydes, esters, methanol, higher alcohols, volatile acidity and furfural acid were determined by gas chromatography coupled to flame ionization detector and the ethyl carbamate by gas chromatography coupled to a mass spectrometer. The sensory analysis was performed using descriptive (Free Choice Profile) and affective (acceptance test and the attitude to purchase of the consumer). With the Free Choice Profile data, Generalized Procrustes Analysis (GPA) was performed. Only the final consensus dimension were the terms descriptors with r ≥ |0.6| in at least one of the first two dimensions and assessors with a maximum residue of 30%. For the acceptance test and chemical characterization data, descriptive statistical analysis, normality and homoscedasticity was performed. The analysis of the External Preference Map (PrefMap) was also carried out to correlate the chemical and sensorial characteristics with the acceptance of the consumers. The chi-square test... (Complete abstract click electronic access below) / Doutor

Orientador: Otávio José de Oliveira / Banca: Aldo Roberto Ometto / Banca: Rosane Aparecida Gomes Battistelle / Resumo: A relação entre a ISO 9001 e a inovação é complexa, necessitando assim, de estudos acadêmicos direcionados. Diante disso, esta dissertação verifica em que aspectos e de que forma o Sistema de Gestão da Qualidade ISO 9001 se relaciona com a inovação em empresas industriais de médio e grande porte do Estado de São Paulo, identificando-se e analisando-se a existências de sinergias e antagonismos entre elas, de maneira a se formularem recomendações para potencializar os resultados do seu trabalho conjunto. O método de pesquisa utilizado foi o estudo de casos múltiplos, sendo que todas as empresas participantes desta investigação eram certificadas com a ISO 9001. Como resultado, identificou-se que a relação existente entre a ISO 9001 e a inovação tem aspectos positivos e negativos, uma vez que em determinados pontos a qualidade influencia beneficamente o processo de inovação e em outros, dificulta a criação de ideias novas. Assim, não há predominância de um ponto mais positivo ou negativo, pois a maximização das relações sinérgicas e o encurtamento dos pontos antagônicos dependem da postura adotada por cada empresa e da direção que essas conduzem suas estratégias de desempenho organizacional. Além dos resultados, este estudo contribuiu com o estabelecimento de recomendações para uso conjunto da qualidade e da inovação a fim de buscar ganhos coletivos simultâneos, nas duas estratégias competitivas / Abstract: The relationship between ISO 9001 and inovation is complex, requiring, thus targeted academic studies. Thus, this dissertation finds that aspects and how the Quality Management System ISO 9001 relaters to inovation in industrial enterprises of medium and large size of the state of São Paulo, identifying and analyzing the existence of synergies and antagonisms between them, so to make recommendations to enhance the results of thei joint work. The research method used was a multiple case study, where all companies participating in this research were certified with ISO 9001. As a result, we found that the relationship between ISO 9001 and innovation has positive and negative aspects, since at certain points the quality beneficially influences the innovation process, and in others, hinders the creation of new ideas. Thus, there is a predominance of one point more positive or negative, for maximizing the synergistic relationships and shortening the points depend on the antagonistic posture adopted by each company and the direction that these strategies lead their organizational performance. Besides the results, this study contributed to the establishment of recommendations for joint use of quality and innovation in order to seek collective gains simultaneous in both competitive strategies / Mestre

With the reliance on information technology and the software that this technology utilizes increasing every day, it is of paramount importance that software developed be of an acceptable quality. This quality can be achieved through the utilization of various software engineering standards and guidelines. The question is, to what extent do these standards and guidelines need to be utilized and how are these standards and guidelines implemented? This research focuses on how guidelines developed by standardization bodies and the unified process developed by Rational can be integrated to achieve a suitable process and version control system within the context of a remote client/contractor small team environment.

Voedseltuisnywerhede (hierna genoem tuisnywerhede) word in 'n toenemende mate van aktuele belang as gevolg van verskeie redes soos die veranderde rol van die hedendaagse vrou en omdat 'n sekere behoefte eie aan vandag vervul word. Bekostigbare voedsel van goeie gehalte is van die uiterste belang. Dit is belangrik om bestanddele van swak gehalte vroegtydig te ontdek voordat produkte van swak gehalte gelewer word en 'n markaandeel verloor word. Tuisnywerhede het 'n verantwoordelikheid teenoor kliente om produkte van goeie gehalte teen bekostigbare pryse te voorsien. Daar word toenemende druk op tuisnywerhede geplaas om produkte van gewenste gehalte te verskaf en om aan kliente se verwagtinge te voldoen. 'n Totalegehaltebestuurprojek kan tuisnywerhede help om hul totale stelsel te ondersoek en te verbeter. In die studie is aile dimensies van die bestuur van gehalte by tuisnywerhede in SuidAfrika ondersoek en geevalueer en 'n TQM model vir tuisnywerhede is ontwikkel. Die probleem is ontleed deur 'n literatuurstudie te doen waarop die empiriese ondersoek gebaseer is. Verskeie inligtingsbronne, in die vorm van artikels, referate en ander geskrewe literatuur oor TQM en tuisnywerhede, is geraadpleeg. 'n Proto-model vir totalegehaltebestuur by tuisnywerhede is uit die literatuur ontwikkel en na die ondersoek aangepas. Die empiriese ondersoek was tweeledig van aard, naamlik op makro- en mikrovlak. Empiriese data is deur middel van posvraelyste aan aile tuisnywerhede in Suid-Afrika verkry. Dieptestudie-ondersoeke deur middel van waarneming, onderhoude en gestruktureerde vraelyste aan tuisnywerheidbestuur, verskaffers en kliente van drie gekose tuisnywerhede is op mikrovlak uitgevoer om hul persepsies oor die gehalte van produkte en bestuur van totale gehalte in tuisnywerhede te ontleed. Die resultate wat uit hierdie studie voortspruit dui daarop dat: • tuisnywerhede wat as 'n handelsko6perasie bedryf word, die meeste voordele bied vir aile partye wat betrokke is. tuisnywerhede met 'n formele gehaltebestuursprogram sonder uitsondering beter resultate toon teenoor die wat nie formele gehaltebestuur toepas nie. • verskaffers se werksfasiliteite nog nooit op enige wyse ge"inspekteer is of vir higieniese praktyke getoets is nie. / Food home industries (hereafter referred to as home industries) are increasingly becoming more important in our society due to several reasons such as the changing role of the modern woman and because a specific need peculiar to today is met. Affordable, high quality food is of the utmost importance. It is essential to identify ingredients of a low standard in good time before poor quality products are supplied and market share is lost. It is the responsibility of home industries to provide the customer with affordable products of a high quality. Home industries are increasingly pressurised to measure up to the customers' expectations and to supply them with top quality products. A total quality management project can assist home industries in examining their total system and can identify areas for improvement. In this study all dimensions of management and quality in home industries were examined and evaluated. A TQM model for home industries was developed. The problem was analysed by means of a literature study on which the empirical investigation was based. Various sources of information such as articles, papers read at conferences and other written literature on TQM and home industries were consulted. A proto model for total quality management for home industries was developed from the literature and was adapted after the investigation. The empirical investigation was executed at both a macro- and a microlevel. Empirical data was obtained through questionnaires distributed to all managers of home industries in South Africa. In-depth studies by means of observation, interviews and structured questionnaires to home industry management, suppliers and customers of three selected home industries were done at microlevel in order to analyse the quality of products and the management of total quality in home industries. The results that emerge from this study indicate that • home industries which are run as trade co-operatives hold more advantages for all parties concerned. home industries which follow a formal quality management programme prove, without exception, to have better results than those which do not implement formal quality management. • the working facilities of suppliers have never been inspected or tested for hygienic practices in any way. / Business Management / DCOM (Sakebestuur)

The aim of this work was to establish a range of suitable process parameters which can be utilized to produce needlepunched nonwoven fabrics for geotextile applications. Nonwoven fabrics were produced from 100% PP, a blend of 50/50% PP/kenaf and 100% kenaf fibres. The depths of needle penetration of 4, 7 and 10 mm, stroke frequencies of 250, 350 and 450 strokes/min and mass per unit area of 300, 600 and 900 g/m2 were utilized for producing the fabrics, on a Dilo loom. The effect of depth of needle penetration, stroke frequency and mass per unit area on the fabric properties, namely, tensile strength, puncture resistance, pore size, water permeability and transmissivity were analysed. In addition, the effect of chemicals, namely, 10% ammonium hydroxide (NH4OH), 10% sodium chloride (NaCl) and 3% sulphuric acid (H2SO4) solutions on degradation of the fabric was also studied. The results have shown that density, thickness and nominal weight of the needlepunched nonwoven fabrics were related to each other and they were influenced by stroke frequency, depth of needle penetration and feed rate of the needlepunching process. The increase in nominal weight of the fabrics also increases thickness and density of the fabrics. The tensile strength and puncture resistance of the fabrics increased with the increases in stroke frequency, depth of needle penetration and fabric mass per unit area. However, lower tensile strength and puncture resistance were achieved in the fabrics produced at lower stroke frequency, lower depth of needle penetration and lower mass per unit area. Bigger pores were resulted in the fabrics produced at lower stroke frequency, lower depth of needle penetration and lower mass per unit area, however, pore size decreased with increases in stroke frequency, depth of needle penetration and mass per unit area. Water permeability depends on the pore size, properties of the fibres, stroke frequency, depth of needle penetration and mass per unit area. Higher tensile strength and higher puncture resistance were achieved in the needlepunched nonwoven fabrics produced from 100% PP fibres, therefore, they are suitable for some load-bearing geotextile applications, such as reinforcement and separation. However, higher water permeability was achieved in the fabrics produced from 100% kenaf fibres, therefore, they are ideal for geotextile applications where good water permeability is required. Higher values for transmissivity were obtained in the fabrics produced from a blend of 50/50% PP/kenaf fibres, therefore they are suitable for drainage applications. The fabrics produced from a blend of 50/50% PP/kenaf fibres achieved better values of tensile strength, puncture resistance, pore size and water permeability in comparison to that produced from 100% PP and 100% kenaf fibres. However, better tensile strength and puncture resistance were achieved in the fabrics produced from 100% PP fibres and bigger pore size and higher water permeability were achieved in the fabrics produced from 100% kenaf fibres. Therefore, it can be suggested that the nonwoven fabrics produced from a blend of 50/50% PP/kenaf fibres can fulfil almost all requirements of geotextile applications, such as, filtration, separation, reinforcement and drainage. The fabrics produced from 100% PP fibres were not damaged or deteriorated when treated with all the three chemicals due to chemical inertness of polypropylene. However, the fabrics produced from a blend of 50/50% PP/kenaf and 100% kenaf fibres were damaged and deteriorated when treated with H2SO4.

As utilizações de dados altimétricos, nesse estudo analisados a partir de modelos digitais de terreno (MDT), são as mais variadas assim como as técnicas de obtenção desse tipo de dado. Pode-se obter MDT por radar de abertura sintética que é utilizado para o levantamento de grandes áreas e de difícil acesso. Também pode-se gerar esse tipo de dado por Fotogrametria, por Topografia utilizando estação total com apoio GNSS (Global Navigation Satellite System). Uma tecnologia mais atual para a obtenção de dados altimétricos é a utilização de tecnologia Lidar (light detection and ranging) que consiste na medição de distância e coordenadas através de um pulso eletromagnético que interage com os alvos. O produto básico dos sistemas que utilizam a tecnologia Lidar, seja em plataformas aéreas ou terrestres, é denominado de nuvem de pontos. Essas nuvens de pontos possuem coordenadas tridimensionais (X, Y e Z) referenciadas ao centro de fase do sensor. Nesse estudo foram realizados experimentos com dois sistemas de varredura a laser terrestre. O primeiro é um sistema de varredura modelo Optech Ilris 3D e outro Faro Focus 3D, nesse estudo denominados de SVLT Optech e SVLT Faro. Assim, o objetivo desse trabalho é estimar a qualidade posicional dos dados altimétricos gerados por dois SVLT. Os estudos foram executados em dois experimentos. No primeiro experimento foram realizados escaneamentos num campo de calibração. Nessa etapa foi gerada uma nuvem de pontos de cada sistema de varredura, e esses dados foram georreferenciados e a partir de pontos de checagem medidos no mesmo e assim foi estimada o controle de qualidade posicional. No segundo experimento, os dois SVLT foram utilizados para efetuar um levantamento topográfico planialtimétrico em um percurso de aproximadamente um quilômetro. Os dados gerados pelos SVLT passaram por um processo de registro, que consiste na junção de pares de nuvens de pontos, e foram georreferenciados a partir de pontos de controle medidos no terreno. Nesse percurso foram realizados circuitos de nivelamento cujos vértices foram utilizados para se estimar o controle de qualidade posicional, planimétrico e altimétrico, das nuvens de pontos oriundas dos dois sistemas de varredura. Assim, após a realização de testes estatísticos, no primeiro experimento, obteve-se valores de acurácia altimétrica de 1,5 cm para o SVLT Faro e de 1,3cm para o SVLT Optech e no segundo experimento acurácia altimétrica de 1,9 cm para o SVLT Faro e de 5,9 cm para o SVLT Optech. / The uses of altimetric data, in this study approached from digital terrain models (TDM) are the most varied as well as the techniques for getting this type of data. It can be get DTM by synthetic aperture radar that is used for surveying wide areas and with difficult access. As well it can be this type of data by Photogrammetry, by conventional Topography using total station with GNSS (Global Navigation Satellite System) support. A more actual technology for obtaining altimetric data is the use of light detection and ranging technology, which consists of measuring distance and coordinates through an electromagnetic pulse that interacts with the targets. The basic product of the systems that use the technology lidar, be it in aerial or terrestrial platforms, is denominated a point cloud. These point clouds have three-dimensional coordinates (X, Y, and Z) referenced to phase center of the sensor. In this study, will be performed experiments with two systems of terrestrial laser scanning (STLS). One STLS of the brand Optech Ilris 3D and another of the brand Faro Focus 3D, in this study called of STLS Faro and STLS Optech. Therefore, the aim of this work is to estimate the positional quality of digital terrain models generated by these two STLS. The studies were executed in two experiments. In the first experiment, scans has been made on a calibration wall. The generated point clouds were georeferenced and from the checkpoints measured in this calibration wall, the positional quality of these data was estimated. In the second experiment, the two STLS were has been made a planialtimetric topographic survey of a course of approximately one kilometer. In this course a leveling network was created and the vertices of this network were used to estimate the positional quality control of the points clouds from the two STLS. Therefore, after realization statistical tests, in the first experiment, it was obtained altimetric accuracy values of 1.5 cm for the STLS Faro and 1.3 cm for the STLS Optech were obtained and in the second experiment, the altimeter accuracy of 1.9 cm for the STLS Faro and 5.9 cm for the Optech STLS.

A atropina é um alcaloide conhecido pela inibição da acetilcolina e como substância antimuscarínica e está presente em folhas de algumas espécies da família Solanaceae, e, em especial, da Atropa belladonna L. A importância da produção de fitoterápicos na indústria farmacêutica tem impulsionado estudos científicos no sentido de desenvolver metodologias analíticas para quantificação de marcadores químicos, presentes em drogas vegetais, e que atendam os parâmetros de controle de qualidade da legislação vigente. Assim, neste trabalho, diferentes metodologias para quantificação da atropina (marcador químico predominante nas folhas de A. belladonna) foram desenvolvidas e validadas. Os métodos propostos por Cromatografia em Camada Delgada de Alta Eficiência (CCDAE), Cromatografia a Líquido de Alta Eficiência (CLAE) e Cromatografia a Líquido de Ultra Eficiência (CLUE) foram validados comprovando ter especificidade/seletividade, linearidade, precisão, exatidão e robustez e adequando-se à legislação vigente. Além disso, diferentes métodos de extração foram propostos. Para o primeiro método de análise (CCDAE), uma extração com ácido diluído (H2SO4 0,5 mol/L), seguida de extração líquido-líquido foi utilizada, resultando em teor médio de 0,2913 % de atropina. Para o segundo (CLAE), uma extração com solvente apolar (metanol p.a.) foi proposta, seguida também de extração líquido-líquido e com teor médio de 0,2660 % do ativo. No terceiro (CLUE), otimização de extração foi realizada através de Desenho Fatorial Fracionado, seguido de Desenho Box-Behnken, resultando em extração realizada pela mistura de solvente apolar e água (metanol 47 %), seguido de uma extração em fase sólida (sílica) e um teor de atropina médio de 0,3343 %. Um extrato otimizado foi analisado nos três métodos, resultando em teores de atropina iguais a 0,2905, 0,3598 e 0,3343 % para CCDAE, CLAE e CLUE, respectivamente. A revisão da monografia do material vegetal, com ensaios de identificação e físico-químicos, juntamente com a metodologia de doseamento por Cromatografia a Líquido de Alta Eficiência foi proposta com o objetivo de atualizar as metodologias que hoje compõe o roteiro de controle de qualidade da espécie na Farmacopeia Brasileira, que está em sua 5° edição. / Atropine is an alkaloid known for acetylcholine inhibition and as antimuscarinic substance and is present in leaves of some species of the family Solanácea, and especially of Atropa belladonna L. The importance of the production of herbal medicines in the pharmaceutical industry has driven scientific studies to develop analytical methods for quantification of chemical markers, present in herbal drugs, and that meet the quality control parameters of the current legislation. Thus, in this work, different methodologies for quantification of atropine (chemical marker predominant in the leaves of A. belladonna) were developed and validated. The methods proposed by High Performance Thin-layer Chromatography (HPTLC), High Performance Liquid Chromatography (HPLC) and Ultra Performance Liquid Chromatography (UPLC) were validated proving to have specificity/selectivity, linearity, precision, accuracy and robustness, and conforming to legislation. In addition, different extraction methodologies were proposed. For the first method of analysis (CCDAE), extraction with dilute acid (H2SO4 0.5 mol/L) followed by liquid-liquid extraction was used, resulting in an average content of 0.2913% atropine. For the second (HPLC), extraction with apolar solvent (methanol p.a.) was proposed, followed also by liquid-liquid extraction and with an average content of 0.2660% of the active. In the third (CLUE), optimization of extraction was done through Fractional Factorial Design, followed by Box-Behnken Design resulting in extraction by the apolar solvent mixture and Water (47% methanol), followed by solid phase extraction (silica) and an average atropine content of 0.3343%. An optimized extract was analyzed in three methods, resulting in atropine levels equal to 0.2905, 0.3598 and 0.3334 % for HPTLC, HPLC and UPLC, respectively. The review of the monograph of the plant material, with identification and physicochemical tests, together with the methodology of determination by HPLC was proposed with the objective of updating the methodologies that today compose the roadmap of quality control of specie in Brazilian Pharmacopoeia, which is in its 5th edition.