Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-04-28T13:43:39Z No. of bitstreams: 1 467666.pdf: 1009580 bytes, checksum: fdf4a9b22505abde150b877e386a5b96 (MD5) / Made available in DSpace on 2015-04-28T13:43:39Z (GMT). No. of bitstreams: 1 467666.pdf: 1009580 bytes, checksum: fdf4a9b22505abde150b877e386a5b96 (MD5) Previous issue date: 2015-03-23 / Conselho Nacional de Pesquisa e Desenvolvimento Cient?fico e Tecnol?gico - CNPq / During the cycle of life of organisms, the food intake is crucial to maintenance and anticipation of biological events. One of the major events in terms of metabolic costs is reproduction. Indicators of oxidative status may become important tools in conservation biology, in order to evaluate survival and reproduction expectations on organisms, as well as to elucidate the effects of anthropic impacts among populations in their natural environment. Tropidurus catalanensis it is a dark-colored and medium size lizard that can be found in rocky outcrops, and has a day-light habit and territorial behavior. It?s reproductive period goes to September from January, with males defending its harem and females tend to be more sedentary. The objective of the present study was to analyze seasonal variations of the intermediate metabolism, body index and biomarkers of the oxidative status of males and females from a population of Tropidurus catalanensis from Pampa biome, which occurs in Argentina, Brazil and Uruguay. The study was developed in a farm located in the city of Alegrete, RS. The study lasted one year, and it was collected 73 animals by active search and manual capture. It was measured the snout-vent-length (SVL), the body weight and it was taken blood samples by cardiac puncture with heparinized syringes.It was analyzed total proteins, uric acid, glucose, total lipids, triglycerides, total cholesterol and VLDL cholesterol of the blood plasma. On the liver, it was analyzed the levels of total proteins, glycogen, total lipids and triglycerides. On the liver, kidneys and muscle tissue from the lizard?s tale it was analyzes the levels of lipid peroxidation (TBARS) and the activities of the following antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST). It was also measured the hepatosomatic and gonadosomatic index, as well as the abdominal fat. The present results indicate that this lizard has a clear pattern of seasonal variation on its metabolism, its oxidative stress biomarkers and its body indexes. The winter period seems to induce a hypometabolic state mainly in males, and the reproductive period seems to be the biological event that requires the largest energetic demands, especially in females. These costs caused by reproduction may increase the vulnerability of the species to any environmental disturbance. Thus, the present study can be considered important on the description and understanding of the effects of the seasonality on the intermediate metabolism and oxidative status of free-living lizards, providing resources for the conservation of herpetofauna. / Ao longo do ciclo de vida dos organismos, a entrada de nutrientes se faz necess?ria para a manuten??o e antecipa??o de eventos biol?gicos. Um dos eventos com maiores custos para o metabolismo ? a reprodu??o. Marcadores do status oxidativo podem tornar-se ferramentas importantes na biologia da conserva??o para avaliar expectativas de sobreviv?ncia e reprodu??o nos organismos, bem como elucidar os efeitos de impactos antr?picos em popula??es em ambiente natural. Tropidurus catalanensis ? um lagarto encontrado em afloramentos de rocha, de porte m?dio e cor escura, com h?bito diurno e comportamento territorialista. O per?odo reprodutivo inicia-se em setembro e se encerra em janeiro, onde machos defendem seus har?ns e f?meas tornam-se residentes. O objetivo do presente estudo foi analisar as flutua??es sazonais do metabolismo intermedi?rio, de ?ndices corporais e marcadores do status oxidativo de machos e f?meas de uma popula??o de Tropidurus catalanensis, que habita os campos do Pampa, bioma que abrange os pa?ses Argentina, Brasil e Uruguai. O estudo foi desenvolvido em uma ?rea localizada em Alegrete, RS. Foram coletados 73 animais ao longo de um ano, atrav?s de busca ativa e captura manual. Houve o levantamento do comprimento rostro-cloacal (CRC), da massa corporal e foram retiradas amostras de sangue atrav?s de pun??o card?aca com o aux?lio de seringas heparinizadas.No plasma foram analisados os n?veis de prote?nas totais, ?cido ?rico, glicose, lip?deos totais, triglicer?deos, colesterol total e colesterol VLDL. No f?gado foram analisados os n?veis de prote?nas totais, glicog?nio, lip?deos totais e triglicer?deos. Nos tecidos hep?tico, renal e por??o do m?sculo caudal foram analisados os n?veis de lipoperoxida??o (TBARS) e a atividade das enzimas antioxidantes super?xido dismutase (SOD), catalase (CAT) e glutationa S-transferase (GST). Tamb?m foram levantados os ?ndices hepatossom?tico, gonadossom?tico e de gordura abdominal. Os resultados obtidos revelam que este s?urio apresenta um claro padr?o de varia??o sazonal em seu metabolismo, dos marcadores de estresse oxidativo e nos ?ndices corporais. Os meses de inverno parecem levar a um estado de hipometabolismo principalmente em machos, assim como o per?odo reprodutivo parece ser o evento biol?gico que exige maior demanda energ?tica, principalmente para as f?meas. Estes custos ocasionados pela reprodu??o podem tornar a esp?cie mais vulner?vel a altera??es em seu meio ambiente. Sendo assim, o presente trabalho pode ser considerado importante na descri??o e entendimento dos efeitos da sazonalidade no metabolismo intermedi?rio e no status oxidativo de lagartos de vida livre, dando subs?dios para a conserva??o da herpetofauna.