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Europäisches Rassen-Monitoring und Pathogenesestudien zur Turcicum-Blattdürre (Exserohilum turcicum) an Mais (Zea mays L.) / European monitoring of physiological races and studies on pathogenesis of Northern Corn Leaf Blight (Exserohilum turcicum) on maize (Zea mays L.).Hanekamp, Hendrik 25 April 2016 (has links)
No description available.
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Molecular Analysis of Oomycete Pathogens to Identify and Translate Novel Resistance Mechanisms to CropsFedkenheuer, Kevin E. 14 July 2016 (has links)
Disease outbreaks caused by oomycetes can be catastrophic. The first part of this dissertation describes development of a system to identify potential new and durable resistance (R) genes against P. sojae in soybean germplasm. We developed a system to screen soybean germplasm for genes that recognize core Phytophthora sojae RXLR effectors that are conserved within the pathogen species and essential for virulence. R genes that recognize these effectors will likely be effective and durable against diverse P. sojae isolates. We developed a system to deliver individual P. sojae effectors by Type III secretion into soybean using the bacterium Pseudomonas, and we screened 12 core effectors on a collection of 30 G. max lines that likely contain new resistance genes against P. sojae. We identified candidate R genes against 10 effectors. Genetic segregation ratios from crosses indicated that three of these genes have a simple inheritance pattern and would be amenable to breeding into elite cultivars. The second part of the dissertation involves use of a model plant-oomycete system to study the genetic basis of susceptibility to oomycete diseases. We compared host transcriptomes from a resistant and a susceptible infection of Arabidopsis thaliana by the downy mildew pathogen Hyaloperonospora arabidopsidis (Hpa). We identified five gene clusters with expression patterns specific to the susceptible interaction. Genes from each cluster were selected and null mutants were tested for altered susceptibility to virulent Hpa. Most A. thaliana null mutants showed enhanced disease susceptibility, suggesting their involvement in pattern-triggered immunity (PTI). A knockout mutant in the AtGcn5 gene was completely resistant to Hpa Emco5 suggesting that the gene/protein is necessary for Hpa to successfully colonize the plant. This study provided new molecular insights into plant-oomycete interaction and revealed a plant gene that could potentially be engineered to provide enhanced resistance to oomycete pathogens. / Ph. D.
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Identification, Validation, and Mapping of Phytophthora sojae and Soybean Mosaic Virus Resistance Genes in SoybeanDavis, Colin Lee 24 May 2017 (has links)
Estimated at approximately $43 billion annually, the cultivated soybean Glycine max (L.) Merr., is the second most valuable crop in the United States. Soybeans account for 57% of the world oil-seed production and are utilized as a protein source in products such as animal feed. The value of a soybean crop, measured in seed quality and quantity, is negatively affected by biotic and abiotic stresses. This research is focused on resistance to biotic disease stress in soybean. In particular, we are working on the Phytophthora soja (P. sojae) and Soybean Mosaic Virus (SMV) systems. For each of these diseases, we are working to develop superior soybean germplasm that is resistant to the devastating economic impacts of pathogens. The majority of this research is focused on screening for novel sources of P. sojae resistance with core effectors to identify resistance genes (R-genes) that will be durable under field conditions. Four segregating populations and two recombinant inbred line (RIL) populations have been screened with core effectors. Effector-based screening methods were combined with pathogen-based phenotyping in the form of a mycelium-based trifoliate screening assay. One RIL population has been screened with virulent P. sojae mycelium. Disease phenotyping has generated a preliminary genetic map for resistance in soybean accession PI408132. The identification of novel R-genes will allow for stacking of resistance loci into elite G. max cultivars. The second project covered in this dissertation describes the validation of the SMV resistance gene Rsv3. Utilizing a combination of transient expression and homology modeling; we provide evidence that Glyma14g38533 encodes Rsv3. / Ph. D.
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Identification of Molecular Markers Associated with the <i>Rps</i>8 locus in Soybean and Evaluation of Microsporogenesis in <i>Rps</i>8/<i>rps</i>8 Heterozygous LinesOrtega, Maria Andrea January 2009 (has links)
No description available.
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Characterization of <i>Rps</i>8 and <i>Rps</i>3 Resistance Genes to <i>Phytophthora sojae</i> through Genetic Fine Mapping and Physical Mapping of Soybean Chromosome 13Gunadi, Andika 19 December 2012 (has links)
No description available.
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