Biological invasions are currently a global problem. They often cause a loss of biodiversity and severely disrupt the balance of entire ecosystems. One of the most dangerous among the many species of crayfish known to be invasive is the marbled crayfish or Marmorkrebs (Procambarus fallax f. virginalis Martin et al., 2010). The marbled crayfish is a major ornamental crayfish species. It is a known vector of infectious crayfish plague, which is lethal for crayfish species of non-North American origin. Marbled crayfish is also very tolerant to different water temperatures. The most important feature making it an especially dangerous invasive species, is the way of its reproduction. Marbled crayfish reproduces via apomictic parthenogenesis. Therefore, only females are able to reproduce and males of this taxon has not been recorded. This crayfish is characterised by a very quick maturation and extremely rapid growth. Even though some established populations of this taxon in the wild have already been recorded, and probability of the establishment in selected regions was evaluated, no predictions for expansion on a global scale have been conducted. The program CLIMATCH was used in this thesis to match the climate conditions between the source area (distribution of established populations of marbled crayfish in the wild) and target areas (all continents excluding Antarctica) based on temperatures during the coldest and warmest quarters of the year. The results showed that marbled crayfish can probably survive and reproduce in even colder regions of the temperate zone. The presented findings additionally indicate that temperate zones contain slightly higher potentially suitable regions than in the case of tropics. There are temperature conditions most suitable for the survival and reproduction of the marbled crayfish within Europe in comparison of the rest of the world; and the least favourable temperature conditions are within Australia. The marbled crayfish introduction and ability to survive and reproduce in the wild is a potential threat to native biota and entire ecosystems on all continents assessed. Therefore, it is strongly recommended that the breeding of and the pet trade with this crayfish should be regulated by policymakers and wildlife managers by improvement of legislative restrictions (such as in the EU) and intensive future education of general public. It is obvious that the monitoring of invasive crayfish species might be continuous. Nevertheless, the most important step is to make producers and hobbyists aware of the risks related with marbled crayfish.

Diplomová práce se zabývá analýzou současné situace ve firmě Sportobchod.cz s.r.o a aplikací teoretických znalostí projektového řízení, nástrojů a metod k návrhu projektu, který má za cíl usnadnit proces budování a zapojení své komunity zákazníků na Slovensku. Kromě toho by projekt měl sloužit jako návod pro následující projekty analyzované společnosti. Diplomová práce je rozdělena do tří hlavních částí. První část je zaměřena na představení teorie projektového řízení a vymezení jeho hlavních konceptů. Druhá část je věnována analýze současné situace, která zahrnuje využití různých makroekonomických a mikroekonomických analýz. V poslední části autor navrhuje projekt pomocí metod projektového řízení jako je SMART definice projektových cílů, logický rámec, WBS, RACI Matice, Ganttův diagram a charakterizuje možné přínosy navrhovaného projektu pro analyzovanou společnost.

The topic of this diploma thesis is an analysis and selection of the new information and point of sale system for groceries divisions of the Inva Group a.s. company. First part of this thesis focuses on analysis of the current IT conditions in the company and on drawing up the requirements for the selection of the information system itself, based on the analysis of the company’s business processes. The second part will be focused on market analysis, and will present different options for information and POS systems and depending on the chosen IS, the timeline and the financial plan of the implementation itself will be created at the end.

This master thesis is focused on assessing the situation regarding the enterprise information system and its processes. Theoretically describes the topic of information systems and their use in the enterprise. The assessment itself is determined by appropriate analysis and on the basis of the requirements of the draft amendment is designed to shape the future information society.

Diplomová práce je věnována aplikaci metod a nástrojů projektového řízení v rámci projektu “You2ber Life!”. Kapitola 1 studovala teoretické základy projektového řízení a jeho metod. Kapitola 2 hodnotí (současná situace, udržitelnost události, konkurenci a shrnutí poznatků získaných pro zlepšení v příštích letech). Kapitola 3 simuluje (finanční analýzu a návrh rozpočtu), definuje (cíl SMART, WBS a řízení času pomocí Ganttova diagramu), vysvětluje (význam lidských zdrojů při řízení projektů), objasňuje (význam lidských zdrojů při řízení projektů), vyhodnocuje (rizika) a skládá se z návrhů platných pro projekt “You2ber Life!”. Nicméně, po úpravách může být použita i v příštích letech.

I have chosen this topic of my paper, because I have been working in Divadlo Husa na provázku as a network administrator. The every day problems I have been contending with should be eliminated by this project as well as it can bring some new pleasant function in whole computer infrastructure. In theoretical part of my paper I will engage in information systems in general and it’s analyse. In a practical part of my paper I will use HOS 8 analyse for evaluation of current IS. After this analyse some variation of improvement will be introduced. In final part of this paper will be the financial calculation of suggested solutions included.

Analysis of the "Automated processing of telephone bills" in a large consulting firm. Use of project management methods: logical framework, analysis of stakeholders, responsibility matrix, milestones chart, PERT analysis.The main objective it to develop the concept, in which it will be better to handle projects in the consulting company in the future. The information for the purposes of this thesis were collected using the method of guided interviews with leading professionals in the company and using the online questionnaire.

Pesticides used in agro-ecosystems and forests enter aquatic environments such as streams, rivers, and lakes if applied in adjacent areas or if an accidental spill occurs. For the studies, we chose the early life stages of common carp (Cyprinus carpio L.) and marbled crayfish (Procambarus fallax f. virginalis). In selected organisms, we observed long-term effect of triazine herbicides (prometryne, terbuthylazine, metribuzine) and their metabolites (terbuthylazine-desethyl and tebuthylazine-2-hydroxy) in concentrations commonly occurring in Czech rivers. The first part is about monitoring the effects of triazines (prometryne, terbuthylazine) on the early development stages of carp and marble crayfish. Subchronic prometryne exposure of early-life stages of common carp at concentrations of 1 200 and 4 000 ?g/L affected their survival, growth rate, early ontogeny, and histology. Terbuthylazine in concentration 1 400 and 3 000 ?g/L caused significant decrease of mass, total length, delayed in development and cause of alternation of tubular system of caudal kidney of carp. Prometryne in concentration 144, 1 444 and 4 320 ?g/L caused decrease of weight, specific growth rates and caused histopathological changes in gill of crayfish. Moreover concentration 4 320 ?g/L of prometryne caused delay in ontogenetic development of crayfish. Second part of the work has included effects of low concentrations of metabolites (terbuthylazine-desethyl and tebuthylazine-2-hydroxy) on the early development stages of carp and marble crayfish. Chronic terbuthylazine-desethyl exposure in concentrations 180, 900, and 1 800 ?g/L affected growth, ontogenetic development, and the antioxidant system and caused pathological changes in the caudal kidney of early life stages of carp. Chronic terbuthylazine-2-hydroxy exposure in concentrations 2.9, 70, 1 400 and 3 500 ?g/L caused decreased of total superoxide dismutase activity of early life stages of carp. Moreover concentration 1 400 and 3 500 ?g/L of terbuthylazine-2-hydroxy caused delay in ontogenetic development and pathological changes in the caudal kidney of carp. Chronic terbuthylazine-2-hydroxy exposure in concentrations up 75 ?g/l affected growth, ontogenetic development, antioxidant system, caused oxidative stress and pathological changes in hepatopancreas of early life stages of marbled crayfish. The last part of our study examined the effect of metribuzine on signal crayfish (Pacifastacus leniusculus Dana). Crayfish were exposed to metribuzine concentrations of 0.52 ?g/L and 3.06 mg/L for 30 days and a 30-day depuration period. In the thiobarbituric acid reactive substances, superoxide dismutase, and catalase were observed differences in all examined tissues (gill, muscle, hepatopancreas) compared to the control group. Differences from control were observed in glutathione reductase activity in hepatopancreas after 10 days for both exposure concentrations and after 30 days at 3.06 mg/L. Histological examination revealed extensive focal autolytic disintegration of tubular epithelium in hepatopancreas of crayfish exposed to metribuzin. These studies provided important results for the evaluation of long-term impact and effect of (prometryne, terbuthylazine and metribuzine) and their metabolites (terbuthylazine-desethyl and tebuthylazine-2-hydroxy) on the water non-target organisms, even in real concentrations founded in surface waters.

The aim of this study was to investigate effects of the triazine herbicide metribuzine on oxidative stress level and antioxidant enzymes activity in gills, muscle and hepatopancreas of signal crayfish (Pacifastacus leniusculus Dana) and also extension of knowledge about effect of metribuzine on the environment. The experiment took up 60 days. Crayfish were exposured to metribuzine concentrations of 0.52 micrograms.l-1 (real environmental concentration) and 3.06 mg.l-1 (10% 96hLC50) for the first 30 days. Then a second phase followed depuration without metribuzine (30 days). Changes in the oxidative stress level (TBARS), superoxiddismutase (SOD) activity and catalase (CAT) activity were observed in all examined tissues. Changes in glutathionreductase (GR) activity were observed only in hepatopancreas. Chronic exposure of metribuzine demonstrated an oxidative damage of cell lipids, proteins and also changes in antioxidant activity in examined crayfish tissues.The results of this study suggest that crayfish are a very suitable organisms for toxicological tests and simultaneously extend knowledge about effect of metribuzine on the environment.

Several studies have been carried out to understand reproductive biology of invasive crayfish. Many researchers have described how invasive species establish their population in new habitats, interactions between native and invasive species, their fast spreading, changing of behavior and biology especially reproductive behavior and even genetics. But it seems that there are still lots of questions and gaps which need to be addressed in case of invasive crayfish species. This thesis tried to focus on different aspects of reproduction biology of invasive crayfish. Generally, the normal crayfish reproduction mode is gonochorism. But, it has been showed in the literature that a few species such as Cherax quadricarinatus, Samastacus spinifrons, Parastacus virilastacus and Pacifastacus leniusculus may have different reproduction modes such as hermaphroditism or intersex. Parthenogenesis has been found in Procambarus fallax f. virginalis. Also, apomictic parthenogenesis has been reported in Orconectes limosus. Moreover, there have been several manipulations which directly affect reproduction biology of crayfish under lab conditions. Additionally, hybridization can be expected between Astacus astacus and Astacus leptodactylus, Orconectes rusticus and Orconectes propinquus under natural conditions. Some studies supposed that chemical factors also one of the reasons which could lead to some changes in reproductive system of crayfish. The objective of the Chapter 2 was to report first evidence of intersex in the signal crayfish, Pacifastacus leniusculus (Dana, 1852). That specimen appeared morphologically male but with both male and female genital openings, as well. Spermophores were obtained from vas defertia of this speciemen. Histological study was carried out and it has shown that both spermatophores and oocytes were present. The gonadosomatic index showed that intersex male had a GSI three times more higher than normal males. The ultrastructure of spermatozoa in three cambarid species Cambarus robustus, Orconectes propinquus, Orconectes rusticus were described and compered with eight previously studied species from family of Astacidae, Cambaridae and Parastacidae. In the studied cambarids, the crest-like protrusions in the anterior part of the acrosome is one of the most remarkable differences in case of morphological feature in spermatozoa and can be used for distinguishing the members of Cambaridae. The results of biometrical data showed that the smallest and biggest acrosome sizes in the studied species were in Parastacidae and Astacidae, respectively. The duration of post-mating spermatophore storage as well as the timing and temperature of spawning in two crayfish species (Pacifastacus leniusculus and Astacus astacus) were investigated in the Chapter 4. Seventy-one pairs of adult signal crayfish (Pacifastacus leniusculus) and thirty six pairs of noble crayfish (Astacus astacus) were used for experiment. The results indicated that there was a significant difference (P<0.05) between noble crayfish and signal crayfish in average duration of the post-mating spermatophore storage. The duration of the post-mating spermatophore storage is longer in the noble crayfish with 34.6 ? 1.7 days than the signal crayfish with 3.9 ? 0.5 days. There were also differences in the timing of mating and egg laying between the signal crayfish and noble crayfish. In the population of signal crayfish both mating and egg laying overlapped, but in the population of noble crayfish there was at least two weeks gap between last mating and first egg laying individuals. Water temperature was significantly (P<0.05) higher during mating and egg laying in the signal crayfish than the noble crayfish. The average temperatures for mating in both species were significantly (P<0.05) higher than the temperatures that they utilized for egg laying. As a conclusion more research is needed for better understand of the reproduction strategies in invasive crayfish.