Understanding the dynamical bases of the interaction between the plant mineral nutrition and the circadian clock could contribute to improve crop yield and resistance to adverse conditions, such as mineral element deficiencies. Magnesium is an essential element that catalyzes more than six hundred enzymatic reactions and occupies the center of the chlorophyll structure in plants. Physiological targets of magnesium deficiency are generally better described in aerial than in belowground organs. In this thesis, we first characterized the root morphology of the model species Arabidopsis thaliana (Columbia-0) in response to magnesium supply during in vitro culture. The length of primary root and the number and length of lateral roots decreased during magnesium depletion. A local magnesium-rich source does not enhance the root foraging capacity, unlike some other major nutrients. Auxin and abscisic acid emerged as two hormones shaping root morphology in response to magnesium deficiency. Second, we investigated the natural variation of the root morphology response to magnesium supply in Arabidopsis. Thirty-six accessions were screened in vitro. Compared to the reference Columbia-0, some accessions had higher number and length of lateral roots at low magnesium supply. Root or shoot magnesium concentrations did not implicitly correlate with the root morphological traits. However, shoot calcium and root phosphorus concentrations correlated positively with the lateral root number and length, while root iron negatively with the length of primary root. Third, we focused on the interaction between the plant magnesium nutrition and the circadian clock circuit. We tested for a possible involvement of PHYTOCHROME INTERACTING FACTOR 3-LIKE 1 (PIL1) - a clock-associated gene that is down-regulated during magnesium depletion - in morphological and physiological responses, and for a circadian connection of PIL1 in the context of magnesium deficiency. The phenotypes of loss-of-function and overexpression lines did not reveal a major role of PIL1 in the magnesium deficiency symptom manifestation but rather in the plant mineral profile. The expression of PIL1 was apparently not under any circadian control. However, PIL1 seemed to regulate the expressions of some core clock genes (CCA1, LHY and PRR9), which were also targets during magnesium deficiency. In conclusion, PIL1 has a link with the circadian rhythm machinery but it does not emerge as a pivotal regulator of magnesium stress responses. / Doctorat en Sciences / info:eu-repo/semantics/nonPublished

Made available in DSpace on 2014-06-11T19:22:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-02-24Bitstream added on 2014-06-13T19:48:26Z : No. of bitstreams: 1 figueiredo_pg_me_botfca.pdf: 864751 bytes, checksum: 77b495899b271a3df8c49ee115f500a4 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Características físicas do solo como porosidade, retenção de água, densidade e resistência a penetração são influenciadas diretamente pelo tipo de preparo do solo. Em resposta a estas modificações ocorrem adaptações morfofisiológicas das raízes. Verifica-se na literatura relatos sobre a influência de diferentes tipos de preparos do solo na produção e na qualidade culinária de raízes de mandioca cultivadas em diferentes tipos de solo, entretanto são desconhecidos os fatores, pertinentes às raízes, que determinam essas diferenças, o que leva a hipótese de que estes fatores possam ser de ordem morfo-anatômica. Diante disso, objetivou-se através deste trabalho de pesquisa estudar a influência de três tipos de preparo do solo (cultivo mínimo, plantio convencional e plantio direto) nas características físicas do solo, produção, morfologia e anatomia de raízes tuberosas de Manihot esculenta Crantz cultivar IAC 576-70. Para isso foi conduzido um experimento a campo em delineamento experimental de blocos ao acaso com parcelas subdivididas e seis repetições. Foram consideradas as causas de variação os tratamentos, preparos do solo, a saber: cultivo mínimo (CM), preparo convencional (PC) e plantio direto (PD) e a idade da planta, em dias após o plantio (DAP). No delineamento experimental os preparos do solo foram considerados parcelas e as idades da planta ou épocas de amostragens as subparcelas, neste experimento foram avaliados a micro, macro e porosidade total, teor de água e resistência a penetração do solo, número, comprimento, diâmetro, massas fresca e seca, porcentagem... / Soil physical characteristics such as porosity, soil water content, bulk density and penetration resistance are directly affected by tillage. In response to these changes occur roots’ morpho-physiological adaptations. In the literature there are reports about the influence of different tillage on the cassava root yields and culinary qualities affected by soil types. However the factors inner roots which determine these differences are unknown, which leads to the hypothesis that these factors may be morphological or anatomical. So the objective of this work was to study the influence of three tillage types (minimum tillage, conventional tillage and no tillage) on soil physical properties, yield, morphology and anatomy of Manihot esculenta Crantz, cultivar IAC 576-70 roots. For those was conducted a field experiment, which experimental design was randomized block in split-plot with six replications. That were considered variation causes, the treatments: minimum tillage (MT), conventional tillage (CT) and no tillage (NT) and plant age, in days after planting (DAP). In the experiment the treatments, tillages, were considered the plots and the subplots were established by plant ages or times of sampling (DAP). In this experiment were evaluated micro, macro and total porosity, soil water content and soil penetration resistance, number, length, diameter, fresh and dry weight, percentage of dry mass, morphological and anatomical characteristics and the proportion of tissues that make up the cassava tuberous roots at different ages of the plant. From the results of these evaluations there was possible to conclude that he order of setting of yield components of sweet cassava cv. IAC 576-70 are independent of tillage, and are defined in the following order: number of root, length, diameter, fresh weight, dry weight... (Complete abstract click electronic access below)

The need to curb increased pollution of environmental resources caused by excessive nitrogen (N) fertilizer application and N fertilizer use inefficiencies in wheat (Triticum aestivum) production systems warrants an inexpensive, sustainable, environmentally sound solution, the root system. Wheat germplasm containing the short arm of rye chromosome 1 (1RS) has recently been found to have larger root system sizes in pot experiments in addition to previously documented higher yields and resistance to leaf, stem and yellow rust. These 1RS lines could therefore be useful in wheat breeding efforts targeting superior root system traits for yield improvements as well as environmental and economic benefits. This dissertation evaluated field root biomass production of Pavon 76 and its wheat-rye (1RS) translocations, effects of root biomass on nitrogen use efficiency, and the temporal variation in their root morphological traits and early growth vigor. The translocation 1RS.1BL had 9 and 23 % higher total root biomass than Pavon 76 at jointing and physiological maturity respectively. Root N uptake peaked at the jointing, where it comprised 22-34% of the total plant N uptake and was lowest at physiological maturity for all genotypes. The inclusion of root N uptake reduced the N utilization efficiency and N harvest index by 6-14 and 7-15% respectively, indicating that the use of only the above ground plant parts over estimates these parameters. In pot experiments, the translocation 1RS .1AL had 12 and 39% higher root biomass than Pavon 76 at anthesis and maturity respectively. 1RS.1BL had 38% higher root mass and 16% longer roots than Pavon76 at physiological maturity. This suggests the existence of differences among the genotypes in below ground partitioning of assimilates at peak nutrient demand (anthesis) for grain filling, and also in rates of root decay and senescence. The lack of differences in root morphological traits among genotypes at early growth stages (6-46 days after sowing) indicates that there are minimal differences in early root growth vigor. The 1RS translocations could therefore expand the wheat breeder's tool box in selections for superior root traits for improved NUE without adverse effects on grain yield.

Orientador: Silvio José Bicudo / Coorientador: Marina Aparecida de Moraes Dallaqua / Banca: Teresa Lousada Valle / Banca: Mario Takahashi / Resumo: Características físicas do solo como porosidade, retenção de água, densidade e resistência a penetração são influenciadas diretamente pelo tipo de preparo do solo. Em resposta a estas modificações ocorrem adaptações morfofisiológicas das raízes. Verifica-se na literatura relatos sobre a influência de diferentes tipos de preparos do solo na produção e na qualidade culinária de raízes de mandioca cultivadas em diferentes tipos de solo, entretanto são desconhecidos os fatores, pertinentes às raízes, que determinam essas diferenças, o que leva a hipótese de que estes fatores possam ser de ordem morfo-anatômica. Diante disso, objetivou-se através deste trabalho de pesquisa estudar a influência de três tipos de preparo do solo (cultivo mínimo, plantio convencional e plantio direto) nas características físicas do solo, produção, morfologia e anatomia de raízes tuberosas de Manihot esculenta Crantz cultivar IAC 576-70. Para isso foi conduzido um experimento a campo em delineamento experimental de blocos ao acaso com parcelas subdivididas e seis repetições. Foram consideradas as causas de variação os tratamentos, preparos do solo, a saber: cultivo mínimo (CM), preparo convencional (PC) e plantio direto (PD) e a idade da planta, em dias após o plantio (DAP). No delineamento experimental os preparos do solo foram considerados parcelas e as idades da planta ou épocas de amostragens as subparcelas, neste experimento foram avaliados a micro, macro e porosidade total, teor de água e resistência a penetração do solo, número, comprimento, diâmetro, massas fresca e seca, porcentagem... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Soil physical characteristics such as porosity, soil water content, bulk density and penetration resistance are directly affected by tillage. In response to these changes occur roots' morpho-physiological adaptations. In the literature there are reports about the influence of different tillage on the cassava root yields and culinary qualities affected by soil types. However the factors inner roots which determine these differences are unknown, which leads to the hypothesis that these factors may be morphological or anatomical. So the objective of this work was to study the influence of three tillage types (minimum tillage, conventional tillage and no tillage) on soil physical properties, yield, morphology and anatomy of Manihot esculenta Crantz, cultivar IAC 576-70 roots. For those was conducted a field experiment, which experimental design was randomized block in split-plot with six replications. That were considered variation causes, the treatments: minimum tillage (MT), conventional tillage (CT) and no tillage (NT) and plant age, in days after planting (DAP). In the experiment the treatments, tillages, were considered the plots and the subplots were established by plant ages or times of sampling (DAP). In this experiment were evaluated micro, macro and total porosity, soil water content and soil penetration resistance, number, length, diameter, fresh and dry weight, percentage of dry mass, morphological and anatomical characteristics and the proportion of tissues that make up the cassava tuberous roots at different ages of the plant. From the results of these evaluations there was possible to conclude that he order of setting of yield components of sweet cassava cv. IAC 576-70 are independent of tillage, and are defined in the following order: number of root, length, diameter, fresh weight, dry weight... (Complete abstract click electronic access below) / Mestre

Softwood cuttings of Euonymus alatus and the cultivar Rudy Haag, a nearly seedless cultivar, were taken in Kentucky on two cutting dates. E. alatus Rudy Haag cuttings taken in mid-May, untreated or treated with 1000 - 3000 ppm IBA, produced many roots on a high percentage of cuttings. Post-rooting shoot growth was reduced for the species with cuttings taken in June. Rudy Haag produced new growth on cuttings taken in May with no IBA treatment. Transplanting resulted in a decrease in new growth. Liner plants of E. alatus Compactus and Rudy Haag were planted into 1 and 3 gallon containers of two different types, conventional black plastic and root training. A second experiment exposed plants to two levels of supplemental fertilizer in addition to slow release. A subsample was sprayed with Fascination (BA + GA4+7) at 1500 ppm in late July. No difference was seen in above-ground growth due to container type or supplemental fertilizer. A change in root morphology is seen with root trainers. Three gallon containers produced a larger plant than one gallon containers. Rudy Haag sprayed with Fascination were greater in size and branch number than those not sprayed.

The aim of this study was to establish a classification system for use in immediate implant placement by examining fresh extraction sockets in maxillary premolars and evaluation of the varying morphologies using CBCT imaging. Selection criteria included dentulous patients ages 15-85 that had CBCT imaging taken for varying treatment. A total of 400 maxillary premolars were classified by their root morphology as Type I (two-rooted premolar with interradicular bone), II (fused roots with mesiodistal alveolar constriction) or III (single blunted root with no interradicular bone). The internal root angle was measured for all Type 1 maxillary first premolars (n=40), as well as the interradicular septal bone height. A Type 1 premolar socket is present in 32% of the sampled maxillary first and second premolars. Type 2 socket was present in 22% of premolars, and type 3 socket was present in 46% of premolars. The average internal angle formed between the long axis of the crown versus the long axis of the palatal root was 11.46 ± 4.35° (range 4° to 20.7°). The average interradicular septal bone height was of 6.9 ± 1.6 mm (range 3.28 to 9.61). Type I root form had the highest incidence at maxillary first premolars sites and has the most alveolar bone available, thus having a higher probability for primary stability. The type III root form is most common in the maxillary second premolar site and has the least amount of alveolar support for immediate implant placement. The angulation and alveolar bone support provided by the palatal root in Type I root form maxillary first premolar sites may provide stability for an immediate implant at an appropriate prosthetic position.

With the increasing scarcity of water resources, soil moisture stress is the single most threat to global soybean production causing extensive yield losses. The objectives of this study were to investigate soil moisture stress effects on all aspects of soybean growth and development processes and to develop functional algorithms that could be used for field management decisions and in soybean crop modeling. To fulfill these objectives, six experiments were conducted; one in vitro osmotic stress study on seed germination, four studies by imposing five soil moisture treatments, 100, 80, 60, 40, and 20% of daily evapotranspiration of the control at different growth stages using sunlit plant growth chambers, and one transgenerational study on seed germination at different osmotic levels and offspring growth at three irrigation treatments (100, 66, and 33% based on field capacity) for plants grown at different soil moisture levels. Two cultivars from maturity group V, Asgrow AG5332 and Progeny P5333RY, with different growth habits were used in all these studies. Midday leaf water potential, plant height, mainstem nodes, gas-exchange traits, canopy reflectance, and several yield components including pod weight, seed yield, and seed quality were measured. Soil moisture stress decreased biomass, net photosynthesis, yield, individual seed weight, maximum seed germination, protein, fatty acids, sucrose, N, and P and increased oil, stachyose, Fe, Mg, Zn, Cu, and B contents. Overall, Asgrow AG5332 was more tolerant to drought stress than Progeny P5333RY. Soil moisture stress induced changes in seed quality that were correlated with seed germination and seedling vigor in the F1 generation. These data can be used to build a model-based decision support system capable of predicting yield under field conditions.

The purpose of this study was to determine the effects of amendments (control, (1x) compost, (2x) compost, (TS) topsoil, and 1x+TS) and microtopography (level, pit and mound) on three parameters (plant species composition, above-ground characteristics of Taxodium distichum, and plant root characteristics) of vegetation growing at a created tidal freshwater swamp in Virginia. None of the soil treatments met the traditional vegetation criteria for federal wetland jurisdictional determination, which only considers dominant species. When the same criteria were used for all of the species, the control, 1x, and 2x treatments met jurisdictional criteria. Considering these findings, vegetative criteria should be re-evaluated for young created wetlands. Compost addition produced the highest proportion of obligate wetland species (30%) while topsoil additions created the lowest proportion of wetland obligates (11%) and the highest proportion of upland plants. The 1x treatment generated the greatest species evenness and lowest weighted average (2.57). Topsoil treatments had the lowest diversity and evenness. Therefore, compost amendment is recommended to increase hydrophytes without compromising evenness and diversity. Bald cypress in pits were taller, had larger trunk diameter and basal trunk swelling than trees growing at higher elevations. Roots growing in mounds were more numerous with greater length than roots at lower elevations. Root length and count were highest for the control soil treatment. Amended treatments may have had lower rooting values because nutrient supply was adequate. Overall, incorporating microtopography and compost during wetland creation had a positive effect on vegetative function in this system. / Master of Science

No description available.

Destacam-se as semelhanças entre os nutrientes boro (B) e cálcio (Ca) quanto às formas e as funções que eles exercem nos vegetais. Partindo do princípio que a interação Ca - B afeta os atributos morfológicos, ultraestruturais e bioquímicos, bem como a nodulação nas plantas leguminosas, objetivou-se com esse estudo determinar a relação Ca:B mais adequada para a produção de grãos e matéria seca de soja, bem como avaliar os efeitos da interação B e Ca na fixação biológica de N2 (atividade da nitrogenase -N-ase [EC 1.18.6.1]), na morfologia de raízes (comprimento e superfície total), anatomia de raízes (nódulos) e de folhas, assim como as avaliações ultraestruturais dos mesófilos foliares, rendimento do teor de óleo nos grãos das plantas de soja (cultivar BRS 284), crescidas em solução nutritiva. Foram avaliadas ainda a produção de massa seca das plantas, as concentrações e os acúmulos de Ca, B, Mg, K e N na parte aérea e raízes. Foi empregado um esquema fatorial 5² incompleto, perfazendo treze combinações de doses de Ca (mmol L-1) e B (µmol L-1), as quais foram distribuídas segundo o delineamento experimental de blocos ao acaso, com quatro repetições. As maiores atividades da N-ase foram obtidas com as maiores doses de Ca e B (7,25 mmol L-1 de Ca e 50 µmol L-1 de B). A menor dose de Ca resultou na inibição da N-ase em cerca de 90%. Os maiores comprimentos e superfícies totais de raízes foram obtidos na combinação de doses de 7,25Ca e 12,5B, enquanto que para a combinação das menores doses de Ca e B observou-se uma redução de cerca de 70% e 60% para comprimento e superficial de raízes, respectivamente. A maior produção de biomassa seca das plantas e de grãos, os maiores acúmulos de Ca, B, N, K e Mg nas folhas e o maior rendimento de óleo foram obtidos com o fornecimento de 4,25 mmol L-1 de Ca e 12,5 µmol L-1 de B, observando-se relação Ca:B na solução nutritiva próxima de 350:1 e nas folhas de 700:1. O teor de óleo nos grãos incrementou cerca de 20% com o aumento das doses de B de 3,125 para 50 µmol L 1. O número de camadas de células do córtex dos nódulos aumentou com o incremento das concentrações de Ca e B na solução. Observou-se ruptura da membrana dos cloroplastos no mesófilo foliar e elevado número de bactérias fixadoras de N2 penetrando o interior dos tecidos nodulares quando as plantas foram submetidas ao menor suprimento de Ca e maior de B. A combinação da menor dose de B junto a maior dose de Ca provocou ruptura da parede celular e o espessamento da lamela média das folhas. O aumento no fornecimento de Ca ameniza os efeitos negativos do baixo suprimento de B na produção de biomassa das plantas e de grãos, no comprimento e superfície de raízes e na atividade da nitrogenase. A relação Ca:B, tanto na solução, quanto no tecido vegetal, não pode ser entendida como parâmetro exclusivo de equilíbrio nutricional / Regarding mineral nutrition of plants, we highlight the similarities between nutrients boron (B) and calcium (Ca) in relation to similar functions they play in plant physiology. However, the mechanisms involved in the interaction between these nutrients are not very well known. Assuming that the interaction Ca - B affects the morphological, ultrastructural and biochemical attributes, as well as the nodulation in leguminous plants, the objective with this study was to determine the optimum Ca:B ratio, in the solution and plan tissue as well for dry matter yield of grain and dry matter of soybean, and to evaluate the effects of B - Ca interaction in the biological N2 fixation (nitrogenase activity - N-ase [EC 1.18.6.1]), root morphology (total root length and total root surface area), root anatomy (nodules), as well as in the ultrastructural evaluation of foliar mesophilic, and yield of oil content of soybean (cultivar BRS 284), grown in nutrient solution. Dry matter yield of plants, Ca-concentrations and amounts of Ca, B, Mg, K and N in shoots and roots were also evaluated. The experimental arrange used was an incomplete 5² factorial, making thirteen combinations of Ca (mmol L-1) and B (µmol L-1) rates, which were set in a randomized block design, with four replications. The highest N-ase activity was obtained with the highest doses of Ca and B (7,25 mmol L-1 of Ca and 50 µmol L-1 of B). The lowest Ca rate resulted in approximately 90% inhibition of N-ase. The highest total root length and total root surface area were obtained with combination of 7,25Ca and 12,5B rates, whereas the combination of the lowest Ca and B rates caused reduction of approximately 70% and 60% on root length and area, respectively. The highest dry matter and grain yield, highest accumulations of Ca, B, N, K and Mg on the leaves and the highest oil content in grains were obtained when 4,25 mmol L-1 of Ca and 12,5 µmol L-1 of B were supplied, resulting in a Ca:B ratio of approximately 350:1 on nutrient solution and 700:1 on leaves. The oil content in grains raised about 20% increasing B rates from 3,125 to 50 µmol L-1. The number of cell layers of nodule cortex raised with increasing Ca and B levels in the solution. It was observed membrane rupture of chloroplasts in leaf mesophyll and high number of N2-fixing bacteria entering the interior of nodular tissues when plants were supplied with the lowest Ca rate (1,75Ca) and the highest B (50B). The combination of the lowest B rate (3,125B) and the highest Ca rate (7,25) caused cell wall rupture and thickening of the middle lamella on the leaves. The increase of Ca levels on the solution mitigates the negative effects of the low B supply in plant biomass and grain yield, in root length and root surface area and in N-ase activity. The Ca:B ratio either in the solution or plant tissue, cannot be understood as an exclusive parameter of nutritional status