Dagens städer växer alltmer och upptar därmed värdefulla odlingsbara marker runtom staden. I och med detta kommer därför förtätning, omvandling och återuppbyggnad av städer genom exempelvis återanvändning av grundkonstruktioner bli en avgörande lösning. I samband med EU-projektet, RuFUS (Reuse of Foundations for Urban Sites), studerades hur olika metoder för återanvändning av grundkonstruktioner kan utföras. Detta har genomförts vid relativt många projekt i andra europeiska länder såsom Tyskland och England, men i Sverige är återanvändning ännu ovanligt. Målet med arbetet har därför varit att undersöka hur vanligt det är med återanvändning av grundkonstruktioner i Sverige och hur detta eventuellt kan utvecklas. Utöver det har arbetets syfte varit att utreda hur framtida nybyggnationer och dess grundkonstruktioner ska förbättras för att möta den fortsatta urbaniseringsproblematiken vi står inför. För att skapa en bakgrundsförståelse angående arbetets problemformulering genomfördes först en litteraturstudie som presenteras i teorikapitlet. Därefter utfördes en enkätstudie och intervjuer vars syfte var att skapa en bild av hur situationen är i Sverige och vilka svårigheter som kan förekomma vid återanvändning av grundkonstruktioner. Resultaten från enkätundersökningen och intervjuer visade att förekomsten av projekt med återanvändning av grundkonstruktioner i Sverige är relativt liten. Orsakerna till detta kan delvis bero på hinder som informationsbrist och okunskap i ämnet. Dessutom framgick det att osäkerheter förkommer kring hur grundkonstruktioner ska återanvändas på ett beprövat och säkert sätt. Framför allt var framhävdes svårigheterna som i förstudien och projekteringen fram, då det ansågs vara problematiskt att hitta underlag om den befintliga grundkonstruktionen. Slutsatserna som har tagits utifrån litteraturstudien och enkätresultaten är att det kommer behöva vidtas ett flertal åtgärder för att underlätta återanvändningen av grundkonstruktioner. Exempelvis behöver dagens metoder som används för att testa den befintliga grundkonstruktionens kapacitet utvecklas så att de går att utföra på ett snabbt och enkelt sätt. Detta skulle i sin tur underlätta framtagandet av en tillförlitlig beräkningsmodell. En annan åtgärd skulle kunna vara att utforma standardiserade metoder som är pålitliga och enkla att tillämpa vid produktionen. Slutligen bör även tillgängliga och informativa relationshandlingar skapas vid nybyggnationer. I och med detta kan förutsättningarna förbättras för kommande projekt som ska återanvända grundkonstruktioner. Genom att vidta samtliga åtgärder skulle troligen fler aktörer inom byggbranschen få bättre kunskap inom ämnet och därmed skulle antalet projekt där grundkonstruktioner återanvänds öka. / Our aim has been to study the presence of reused foundations in Sweden and how it would be possible to improve these projects. Furthermore the purpose has been to examine how future constructions and its foundations will be evaluated and designed to meet the obstacles of urbanization we face. The thesis is divided into a theory chapter consisting of a literature study, to create an understanding and to further perceive the aim. The results from the thesis is presented in a chapter which consists of an inquiry and interviews with key persons on this topic and further analyze of the potential and challenges with the reuse of foundations. The results from the survey showed that the presence of reused foundations in Sweden is rather low. The causes of this can be multiple, such as lack of information regarding the technique and unawareness of its existence. It is also apparent that there are uncertainties how to reuse the foundations in a proven, tested and safe technique. The study suggested that another main challenge is finding relevant data regarding the existing foundation.In conclusion, the results of the survey and the literature study show that there are a number of measures which ought to be taken to create awareness and to simplify the reuse of foundations. It can be to create accessible and informative built documents which would facilitate the produce of reliable calculations. With the formulations of standardized methods which would be reliable and safer for the reuse of foundations as well as the produce and accessibility of more information for these kinds of projects.

No description available.

Ce mémoire propose l’analyse de chansons extraites du diptyque Want (formé des albums Want One et Want Two), de l’auteur-compositeur-interprète Rufus Wainwright, en regard des relations entre l’énonciateur et l’espace. La notion d’oscillation spatiale, définie en introduction, est au cœur de la situation de l’énonciateur et se déploie sous de multiples formes dans les cinq chansons à l’étude. « Oh What a World » et « I Don’t Know What It Is » sur Want One, « Hometown Waltz », « Memphis Skyline » et « Old Whore’s Diet » sur Want Two, ont été choisies pour la façon dont la relation à l’espace y agit de même que pour leur emplacement dans l’ensemble. La position de l’énonciateur mise en évidence dans chaque analyse s’inscrit au sein d’un parcours, que l’étude préliminaire du paratexte des albums aura d’ailleurs mis en évidence. / In this dissertation are analysed five songs from the singer-songwriter Rufus Wainwright’s diptych Want (composed of Want One and Want Two), with regards to the relationship between the protagonist of the songs and the space. The notion of « spatial oscillation », of which a definition is given in the introduction, is central to the protagonist’s conflicting state and adopts various forms in the five songs analysed. « Oh What a World » and « I Don’t Know What It Is » on Want One, « Hometown Waltz », « Memphis Skyline » and « Old Whore’s Diet » on Want Two, have been selected for the way in which they deal with spatial relations, as well as for their location in the diptych. The protagonist’s position, highlighted in the five analyses, evolves throughout the diptych ; the preliminary analysis of the paratextual apparatus underlines this progression.

Thesis advisor: Alan Rogers / The following narrative traces the political lives of James Sullivan, Christopher Gore, Rufus King and John Quincy Adams, four Massachusetts men who were actively involved in the creation of state and national policy during the formative years of the new republic. Their years of public service bridged the critical period between the Revolution and the period of Democratic- Republican dominance. Because they knew each other so well, corresponded with one another on a regular basis, and held so many different state and national government posts, their lives provide an ideal vehicle to explore and better understand the changes that were taking place in post-Revolutionary Massachusetts. Their stories help trace the evolution of Massachusetts from a Federalist stronghold into a legitimate multi-party state firmly committed to the national union. The primary figure in this study is Sullivan, the oldest of the four men, who was the state's highest ranking Republican leader during much of the Federalist Era. A staunch opponent of the Federalist assumption that government should be in the hands of the natural gentry and ruling class, he spent his adult life promoting equal access to power. After serving as a member of the Massachusetts Provincial Congress from 1774 to 1776, Sullivan was an active participant in the creation of the new state government. He later served as attorney general for seventeen years, from 1790 to 1807, through several Federalist administrations and served as a member of state legislature for many years. He also was a member of the Supreme Judicial Court and, in the final years of his life, governor of the Commonwealth. Because he participated in or observed firsthand the most significant political events of his day, his words also help trace, as few others could, the gradual transformation of Massachusetts from a one party state to a multi-party state. His election as governor in 1807 was clear evidence of the growing strength of the Republican Party in Massachusetts and of the extent to which the emerging national consensus had grown. Christopher Gore, whose stature and perspective were more deeply rooted in the colonial past, stood in stark personal as well as political contrast to Sullivan. As a conservative Federalist who often served as spokesman for his party during this period, Gore was a major player in the Massachusetts legal community and government between the American Revolution and early years of the nineteenth century. He stubbornly adhered to the aristocratic belief that the government should only be managed by the propertied class and traditional ruling elite. Where Sullivan was a sentimental moralist who hated everything British, Gore was the stern and unyielding spokesman for the merchant class who seemingly admired everything British. Where Sullivan's father had emigrated from Ireland, the victim of oppressive Penal Laws, Gore's father was a Tory, who fled Boston with the British in March 1776. Though Gore himself supported the Revolution, he was never able to shed, or indeed temper, his attachment to Great Britain in later years. As perhaps the most passionate defender of everything British in the years after the Revolution, Gore's habits and customs reflected the old deferential order and embodied everything Sullivan opposed. Despite their personal and political differences, Sullivan and Gore shared a close personal friend. Rufus King was a longtime confidant of both men, corresponding with each of them over many years. Though King's habits and background were more similar to those of Gore than Sullivan, he was less rooted in the colonial past than his conservative friend. Though an ardent Federalist, he was respected by men on both sides of the political aisle and served not only as a bridge between the two parties, but as a bridge between the two branches of his own party. It is because he enjoyed such a close personal relationship with Sullivan and Gore, and corresponded with both men on a regular basis, that King provides a unique vehicle to explore the differences between the two parties during this critical period in Massachusetts political history. The fourth subject of this study is John Quincy Adams. The fiercely independent one-time Federalist, who, though born many years after Gore, King, and Sullivan, became active in politics at a very young age and crossed political paths with all three men on a regular basis. Although born a member of the second generation of political leaders, Quincy Adams identified with the first generation of Revolutionary leaders. He matured early and took part in every critical debate that took place after the ratification of the Constitution. From the beginning, Quincy Adams charted an independent course and played a critical role in the growth of the Republican Party. John Quincy Adams is particularly relevant to this study because his political transformation reflected the change in attitude that was taking place in Massachusetts and the country in the early years of the nineteenth century. He represented a commitment to the interests of union over sectional concerns. A strong and independent unionist throughout his life, Quincy Adams eventually came to represent a new global nationalism. In many respects, Quincy Adams was the `transition man' in post- Revolutionary America. The son of a colonial who was very much a product of the deferential society of the eighteenth century, young Adams came to embrace the principle of majority rule. His elevation to the highest political posts in the country marked the final stage in America's transition from colony to union to nation. James Sullivan, Christopher Gore and Rufus King each played significant roles in the establishment of constitutional government in Massachusetts and in the United States. Though he was considered a member of the so-called Hancock faction, a group viewed as primarily anti-Constitutionalist, Sullivan was an independent thinker. He would call for greater legal safeguards for the benefit of the more vulnerable and for the end of the practice of multiple office holding which had long been a tool of the ruling elite to maintain power and influence.   A vocal proponent of the national government before King, Gore and Hancock, Sullivan had long recognized the importance of strengthening the central government. His embrace of participatory government and of law aimed at protecting all classes of people naturally appealed to a wider audience would continue to contribute to the democratization of Massachusetts politics. With a new national government in place and a new political era begun, Sullivan, King, Gore, and soon Quincy Adams, were uniquely positioned to play significant, if competing, roles in the coming struggle. This narrative differs from other secondary works on post-colonial Massachusetts in several respects. Firstly, the significant role played by Sullivan in the growth of Republicanism in Massachusetts has been largely overlooked by historians. His persistent calls for equal access to power stood in stark contrast to the views of the Federalists who dominated Massachusetts government in the years after the American Revolution. His active participation in regional politics both during and after the Revolution helped the people of Massachusetts in their transition from colony to state. Furthermore, he was one of the first Massachusetts political leaders to insist on placing the new central government on a sound financial footing. Indeed, his call for a strengthened and sufficiently financed national government predated the efforts of Massachusetts Federalists, including King and Gore. He was, I contend, one of the first political leaders of either party to be considered a true `nationalist.' While Quincy Adams' support for Jefferson's Embargo and his conversion to Republicanism have been well documented, this work explores the link between Sullivan and Quincy Adams, and details the critically important role they played in the national debate over how to respond to British aggression towards American shipping and American sailors. Though Gordon Wood and other historians point to the Embargo as the single biggest failure of Jefferson and his Republican supporters, I contend the opposite is true. The Embargo highlighted the central difference between the two parties, and though it provided Federalists with a temporary victory, it also sowed the seeds of their defeat. The Embargo enabled men like Sullivan and Quincy Adams to clarify one of the central issues of the post-Revolutionary period, ... national honor. Though Paul Goodman correctly points out that Republicanism tapped into the growing sense of nationalism in the country, I carry the discussion further and detail the growing disconnect between the Federalist Party and the American people. Quincy Adams, in particular, articulated the need to announce to the world that the United States would not submit to foreign aggression. Furthermore, his call for a stronger and expanded union, even if it meant a loss of power and prestige for Massachusetts, would soon strike a chord with a growing majority of Americans. Quincy Adams personified the shift in the national mood and represented a new national perspective. When John Quincy Adams left the Federalist Party, many Americans left with him. / Thesis (PhD) — Boston College, 2011. / Submitted to: Boston College. Graduate School of Arts and Sciences. / Discipline: History.

Thesis advisor: Gabor T. Marth / To help improve the analysis of forward genetic screens, we have developed an efficient and automated pipeline for mutational profiling using our reference guided tools including MOSAIK and FREEBAYES. Studies using next generation sequencing technologies currently employ either reference guided alignment or de novo assembly to analyze the massive amount of short read data produced by second generation sequencing technologies; the far more common approach being reference guided alignment due to the massive computational and sequencing costs associated with de novo assembly. The success of reference guided alignment is dependent on three factors; the accuracy of the reference, the ability of the mapper to correctly place a read, and the degree to which a variant allele differs from the reference. Reference assemblies are not perfect and none are entirely complete. Moreover, read mappers can only map reads in genomic locations that are unique enough to confidently place reads; paralogous sections, such as related gene families, cannot be characterized and are often ignored. Further, variant alleles that drastically alter the subject's DNA, such as insertions or deletions (INDELs), will not map to the reference and are either entirely missed or require further downstream analysis to characterize. Most importantly, reference guided methods are restricted to organisms for which such reference genomes have been assembled. The current alternative, de novo assembly of a genome, is prohibitively expensive for most labs requiring deep read coverage from numerous different library preparations as well as massive computing power. To address the shortcomings of current methods, while eliminating the costs intrinsic to de novo sequence assembly, we developed RUFUS, a novel, completely reference-independent variant discovery tool. RUFUS directly compares raw sequence data from two or more samples and identifies groups of reads unique to one or the other sample. RUFUS has at least the same variant detection sensitivity as mapping methods, with greatly increased specificity for SNPs and INDEL variation events. RUFUS is also capable of extremely sensitive copy number detection, without any restriction on event length. By modeling the underlying k-mer distribution, RUFUS produces a specific copy number spectrum for each individual sample. Applying a Bayesian detection method to detect changes in k-mer content between two samples, RUFUS produces copy number calls that are equally as sensitive as traditional copy number detection methods with far fewer false positives. Our data suggest that RUFUS' reference-free approach to variant discovery is able to substantially improve upon existing variant detection methods: reducing reference biases, reducing false positive variants, and detecting copy number variants with excellent sensitivity and specificity. / Thesis (PhD) — Boston College, 2014. / Submitted to: Boston College. Graduate School of Arts and Sciences. / Discipline: Biology.

Urban landscapes are quickly replacing native habitat around the world. As wildlife and people increasingly overlap in their shared space and resources, so does the potential for human-wildlife conflict, especially with predators. Bobcats (Lynx rufus) are a top predator in several urban areas across the United States and a potential contributor to human-carnivore conflicts. This study evaluated the movements and habitat use of bobcats in the Dallas-Fort Worth (DFW), Texas metroplex. Spatial data were collected from 10 bobcats via Global Positioning Satellite (GPS) for approximately one year. Average home range size was 4.60 km2 (n=9, SE=0.99 km2) for all resident bobcats, 3.48 km2 (n=5, SE=1.13 km2) for resident females, and 6.00 km2 (n=4, SE=1.61 km2) for resident males. Resource selection function (RSF) models show that bobcats avoid areas close to and far from grasslands and low-medium development, while selecting for these areas at intermediate distances. Bobcats also selected areas closer to developed open space, agricultural areas, and railroads. In addition, camera trap data analyzed with spatially explicit capture-recapture (SECR) models informed by the RSF results estimated a population density of 0.64 bobcats/km2 (SE = 0.22). Bobcats in DFW have significantly smaller home ranges and occur at higher densities compared to rural bobcat populations. Home ranges were also slightly smaller and densities higher than the most closely similar peri-urban bobcat studies. These differences likely arise due to the abundant urban prey species the DFW landscape provides despite limited space and habitat for bobcats. The dense urban development surrounding this population of bobcats may also discourage dispersing from the area, and contributing to higher densities. These results provide information to facilitate management of urban bobcats by providing new insight into how bobcats live amidst people in urban areas.

Remote cameras have become a popular tool for monitoring wildlife. We used remote cameras to estimate bobcat (Lynx rufus) population abundance on the Utah Test and Training Range during two sample periods between 2015 and 2017. We used two statistical methods, closed capture mark-recapture (CMR) and mark-resight Poisson log-normal (PNE), to estimate bobcat abundance within the study area. We used the maximum mean distance moved method (MMDM) to calculate the effective sample area for estimating density. Additionally, we captured bobcats and estimated home range using minimum convex polygon (MCP) and kernel density estimation (KDE) methods. Bobcat abundance on the UTTR was 35-48 in 2017 and density was 11.95 bobcats/100 km2 using CMR and 16.69 bobcats/100 km2 using PNE. The North Range of the study area experienced a decline of 36-44 percent in density between sample periods. Density declines could be explained by natural predator prey cycles, by habituation to attractants or by an increase in home range area. We recommend that bobcat abundance and density be estimated regularly to establish population trends.To improve the management of bobcats on the Utah Test and Training Range (UTTR), we investigated bobcat (Lynx rufus) habitat use. We determined habitat use points by capturing bobcats in remote camera images. Use and random points were intersected with remotely sensed data in a geographic information system. Habitat variables were evaluated at the capture point scale and home range scale. Home range size was calculated using the mean maximum distance moved method. Scales and habitat variables were compared within generalized linear mixed-effects models. Our top model (AICc weight = 1) included a measure of terrain ruggedness, mean aspect, and land cover variables related to prey availability and human avoidance.

No description available.

Identifying the best control method for problematic wildlife is an ever present issue in wildlife management. Popular control methods have ranged from lethal techniques, extirpating the animal, to multiple non-lethal methods focused on deterring undesired behavior. In the past, lethal methods were the preferred choice. However, with increased awareness of the need for biodiversity conservation, new management methods focus on non-lethal control, with emphasis on exploiting aspects of naturally occurring organismal behaviors and ecology. Over the past decade, technological advances in extraction method’s and equipment have also developed new techniques providing a broader range of information about species biology for management use. One of the most well documented conflicts between wildlife and humans is that of the wolf. Using advanced technology and new techniques, we investigated the implication of using chemosensory signals in canid urine to modify behavior as a possible non-lethal alternative in large predator management. Here we used the SBSE method coupled with improved GC/MS equipment to analyze the volatile organic compounds in the urine of four canid species, gray wolf (Canis lupus), red wolf (Canis rufus), wolf-dog hybrids (Canis familiaris) and the domestic dog (Canis familiaris) in order to create working urinary profiles. The extraction method identified several compounds also seen in the urinary profiles of other large predators. In addition, similarities and differences were also noted between taxa and the sexes, and these can be further explored in future studies. Two identified urinary compounds, acetophenone and methyl propyl sulfide, were selected for further behavioral evaluation. We focused on these compounds and their influence as chemosensory signals triggering urine marking events in both the gray wolf and red wolf. Behavioral observations of the effects of these two chemicals indicated they elicited responses from captive wolves. At each of the three study sites, the combination of these chemicals produced urine-marking events along the territory boundary by dominant animals. As a result, the investigation focused on what triggered the urine-marking events, the chemicals themselves, their combination, or the breakdown of the chemicals producing other odorants. It was found that there was no significant degradation of the chemicals over time and environmental conditions produced no significant breakdown of the acetophenone prior to the addition of methyl propyl sulfide. This posed a number of new questions and illustrated the need for additional behavioral studies. The results of this study analyzing chemosensory signals in canid urine, provides biologists with new information to aid in the development of new non-lethal management strategies for handling problematic wildlife as well as providing useful information for future research involving reproduction, predator/prey dynamics, territory maintenance, and a host of other studies focusing on animal ecology in association with chemosensory signaling.

While studying packs of the eastern timber wolf in Algonquin Provincial Park in Ontario, DNA profiles at 8 microsatellite loci and the mitochondrial control region were found to be similar to those of the red wolf, C. rufus. Based on this it was suggested that both the red wolf and the eastern timber wolf have a common origin, evolving in North America, with the coyote diverging from them 150,000-300,000 years ago and with neither having any recent connection with the gray wolf that evolved in Eurasia. It was further proposed, that the eastern timber wolf retain its original species designation of C. lycaon instead of the present status of a subspecies of the gray wolf. Four "types" or "races" of wolves have been previously described in Ontario. Using DNA profiles, assignment tests identified four groups, which were typified by animals in Algonquin Provincial Park, Pukaskwa National Park, Frontenac Axis and those north of Lake Superior. The tests indicate that Frontenac animals are hybrids between the western coyote and C. lycaon and represent the eastern coyote. Pukaskwa maintains a small wolf population, which is genetically closer to the gray wolves of the Northwest Territories than the surrounding C. lycaon. These may represent an isolated remnant population of the original "Ontario type" (C. lupus). Animals north of Lake Superior were identified as C. lycaon, but represent products of hybridization between C. lycaon and C. lupus. Currently within Ontario, Algonquin Park contains the largest protected area of the eastern timber wolf. DNA profiles, including Y-linked microsatellite loci were used to establish maternity, paternity and kin relationships for 102 animals from 24 packs over a 12-year period. A complex pack structure was identified. A pack is not composed simply of an unrelated breeding pair and their offspring and subordinates appear to enter pack systems through adoption, pack splitting, dispersal and immigration. Relatively high genetic structuring was found between the Park animals and the "Tweed" wolves to the southeast suggesting introgression of coyote genetic material is not a present concern to the integrity of park animals. Evidence of gene flow with animals to the west, northeast and northwest coupled with the high genetic diversity, suggest that the Park animals are not an island population, but the southern part of a larger metapopulation of C. lycaon. Increased interest in the relationship of the red and eastern wolves led to the investigation of a gene in the major histocompatibility complex. Allelic variation in the exon 2 region of the DLA-DQA1 locus was analysed for gray wolves, red wolves, the eastern timber wolf and the western coyote. Twelve alleles were identified, seven of which were previously characterized in dogs. Non-synonomous nucleotide substitutions was 3.0 times higher than the synonomous changes, indicative of strong positive selection. These data provide baselines for the determination of allele frequencies and their distribution across the geographical range of the four species in North America. The results in this thesis have sparked numerous debates with respect to the protection of the wolves in Algonquin Provincial Park and reintroduction of wolves into Northeastern United States. The data support the idea that the C. lycaon population in Ontario is relatively large, numbering in the thousands rather than the hundreds. Concern for the conservation of wolves in Ontario should be directed at the declining numbers of gray wolves present in Ontario. / Thesis / Master of Science (MSc)