Made available in DSpace on 2014-12-17T14:13:35Z (GMT). No. of bitstreams: 1 BernardoMR.pdf: 260986 bytes, checksum: 17a4b2567679fec24073be98451b0953 (MD5) Previous issue date: 2008-03-25 / Blood constituents labelled with technetium-99m (99mTc) has been used with radiobiocomplexes in several procedures in nuclear medicine. Some natural and sintetic drugs are capable to interfere on the labeling of blood constituents with 99mTc, on the morphology of red blood cells (RBC) and on the biodistribution of radiobiocomplexes. The aim of this study was evaluate the effect of an extract of Passiflora edulis f. flavicarpa on the labeling of blood constituints with 99mTc, on the morphology of RBC and on the biodistribution of the radiopharmaceutical sodium perthecnetate in Wistar rats. On the in vitro studies the Passiflora edulis f. flavicarpa decreased significantly (p<0.05) the %ATI on plasma proteins and on the in vitro morhology of RBC, the passion fruit peel flour altered the shape and the perimeter/?rea ratio. On the in vivo estudies the extract did not altered the %ATI in blood constituents, and did not altered the shape of RBC. Although, on the biodistribution of the radiobiocomplex sodium perthecnetate (Na99mTcO4) this extract decreased significantly (p<0.05) the uptake in duodenum, spleen, p?ncreas and blood, and increased the uptake in stomach. It can be suggested that the effects presented by this extract could be a result of some substances contained in this extract that could alter the binding of 99mTc to plasma proteins, the morphology of RBC and the biodistribution of the radiobiocomplex sodium perthecnetate. This study was multidisciplinary experimental research. It was developed with the contribution of different Departments and Services of the Hospital Universit?rio Pedro Ernesto of the Universidade Estadual do Rio de Janeiro / Constituintes sang??neos marcados com tecn?cio-99m (99mTc) t?m sido utilizadas com radiobiocomplexos em procedimentos da Medicina Nuclear. Determinadas drogas naturais ou sint?ticas s?o capazes de interferir na marca??o de estruturas sang??neas com 99mTc, na morfometria de hem?cias e na biodisponibilidade de radiobiocomplexos. O objetivo deste estudo foi avaliar o efeito de um extrato de farinha da casca do maracuj? (Passiflora edulis f. flavicarpa) na marca??o de constituintes sangu?neos com 99mTc, na morfologia de hem?cias marcadas com 99mTc e na biodisponibilidade do radiobiocomplexo pertecnetato de s?dio. Nos estudos in vitro, o extrato foi capaz de diminuir significativamente (p<0,05) a fixa??o do 99mTc nas prote?nas plasm?ticas e tamb?m alterar a forma e a rela??o per?metro/?rea de hem?cias. Nos estudos in vivo, o extrato de farinha da casca de maracuj? n?o alterou a marca??o dos constituintes sangu?neos com 99mTc, assim como a forma de hem?cias marcadas com 99mTc. No estudo da biodisponibilidade do radiobiocomplexo pertecnetato de s?dio (Na99mTcO4), o extrato estudado aumentou significativamente (p<0,05) a capta??o no duodeno e ba?o, e diminuiu a capta??o do Na99mTcO4 no p?ncreas, est?mago e sangue. Os efeitos apresentados por este extrato podem ser derivados de subst?ncias presentes no extrato, que poderiam alterar a liga??o do 99mTc ?s prote?nas plasm?ticas, induzir altera??es morfol?gicas nas hem?cias e/ou a biodisponibilidade do radiobiocomplexo pertecnetato de s?dio.. Este trabalho foi desenvolvido em diferentes Departamentos e Servi?os da ?rea biom?dica do Hospital Universit?rio Pedro Ernesto, UERJ, atestando o car?ter multidisciplinar da pesquisa

Made available in DSpace on 2014-12-17T14:13:46Z (GMT). No. of bitstreams: 1 Raquel Terra Agostinho_DISSERT.pdf: 212655 bytes, checksum: afa93fe07c8ba1db5dc83395a1dc586d (MD5) Previous issue date: 2009-09-29 / Clinical evaluations have been made possible with radiobiocomplexes marked with tecnecium-99m (99mTc). Natural or synthetic drugs are able to interfere in the marking of blood structures with 99m Tc. Also, the toxicity of several natural products has been described. The aim of this study was evaluating the effect of an extract of Ganoderma lucidum (Reishi) in the marking of blood constituents with 98m Tc and in the survival of Escherichia coli. Blood samples from Wistar rats were treated with reishi extract. Radiomarking procedure was performed. Samples of plasma (P), blood cells (CS), and insoluble (FI) and soluble (FS) fractions of P and CS were separated and the radioactivity was counted to determine radioactivity percentages (%ATI). Escherichia coli AB1157 cultures were treated with stannous chloride in the presence and absence of the reishi extract. Blood samples and bacterial cultures treated with NaCl 0.9% were used as controls. Data indicated that the reishi extract has significantly altered (p<0,05) the %ATI of P, CS, FI-P, FS-P, FI-CS e FS-CS, as well as it has increased survival of bacterial cultures treated with stannous chloride. Our results suggest that the Reishi extract would be able to present a redox/ chelant action by altering blood constituent marking with 99mTc and by protecting bacterial cultures against stannous chloride-induced oxydating lesions. The study had a multidisciplinary character, with the participation of the following areas of knowledge: Biophysics, Radiobiology, Botanics, Phytotherapy, and Hematology / Avalia??es cl?nicas t?m sido poss?veis com radiobiocomplexos marcados com tecn?cio-99m (99mTc). Drogas naturais ou sint?ticas s?o capazes de interferir na marca??o de estruturas sangu?neas com 99mTc, e tamb?m tem sido descrita a toxicidade de v?rios produtos naturais. O objetivo deste estudo foi avaliar o efeito de um extrato de Ganoderma lucidum (Reishi) na marca??o de constituintes sangu?neos sang??neas com 99mTc e na sobreviv?ncia de Escherichia coli. Amostras de sangue de ratos Wistar foram tratadas com extrato de reishi. O procedimento de radiomarca??o foi realizado. Amostras de plasma (P), c?lulas sang??neas (CS) e fra??es insol?vel (FI) e sol?vel (FS) de P e CS foram separadas e a radioatividade foi contada para determina??o das porcentagens de radioatividade (%ATI).Culturas de Escherichia coli AB1157 foram tratadas com cloreto estanoso na presen?a e aus?ncia do extrato de reishi. Amostras de sangue e culturas bacterianas tratadas com NaCl 0.9% foram usadas como controles. Dados indicaram que o extrato de reishi alterou significativamente (p<0,05) a %ATI de P, CS, FI-P, FS-P, FI-CS e FS-CS, bem como, aumentou a sobreviv?ncia de culturas bacterianas tratadas com cloreto estanoso. Nossos resultados sugerem que o extrato de Reishi poderia apresentar a??o redox/quelante alterando a marca??o de constituintes sang??neos com 99mTc e protegendo culturas bacterianas contra les?es oxidativas induzidas pelo cloreto estanoso. O estudo teve car?ter multidisciplinar com a participa??o das seguintes ?reas do conhecimento: Biof?sica, Radiobiologia, Bot?nica, Fitoterapia e Hematologia

Made available in DSpace on 2014-12-17T14:13:22Z (GMT). No. of bitstreams: 1 SebastiaoDSF.pdf: 475002 bytes, checksum: d28adb2821adc67501d7e19f42e9b6a3 (MD5) Previous issue date: 2007-08-16 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Several clinic evaluations have been possible with radiobiocomplexes labeled with technetium-99m (99mTc). Some natural and synthetic drugs are capable of to interfere on the labeling of blood constituents with 99mTc, as well as on the biodistribution of radiobiocomplexes. Authors have also reported about the toxicity of several natural products. The aim of this study was to compare the effects of the Mentha crispa (hortel?) and of the Hypericum perforatum (hip?rico) in different experimental models. On the labeling of red blood cells (RBC) and plasma and cellular proteins with 99mTc, both extracts were capable of to decrease the radioactivity percentage on the cellular compartment and on the fixation on plasma and cellular proteins. On the morphometry of the RBC, only the hortel? was capable to alter the shape and the perimeter/area ratio of the RBC. On the biodistribution of the radiobiocomplex sodium pertechnetate (Na99mTcO4), the hortel? increased the Na99mTcO4 distribution in the kidney, spleen, liver and thyroid, meanwhile the hip?rico decreased the Na99mTcO4 distribution in the bone, stomach, lungs and thyroid, and increased the Na99mTcO4 distribution in the pancreas. On the bacterial cultures survival, the hip?rico was capable of to protect the bacteria against the stannous chloride (SnCl2) effect. The hip?rico did not alter the topology of plasmidial DNA and did not protect the plasmidial DNA against the SnCl2 action. Probably, the effects presented by both extracts could be due to chemical compounds of the extracts that could alter the morphology of the RBC and the plasma membrane ions transport, and/or by phytocomplexes that could be formed with different effects dependent on the biological system considered / Avalia??es cl?nicas t?m sido poss?veis com radiobiocomplexos marcados com tecn?cio-99mTc (99mTc). Drogas naturais ou sint?ticas s?o capazes de interferir na marca??o de estruturas sangu?neas com 99mTc, assim como na biodistribui??o de radiobiocomplexos. Tamb?m tem sido descrita a toxicidade de v?rios produtos naturais. O objetivo deste estudo foi comparar o efeito dos extratos de Mentha crispa (hortel?) e de Hypericum perfloratum (hip?rico) em diferentes modelos experimentais. Na marca??o de estruturas sang??neas com 99mTc verificou-se que ambos os extratos foram capazes de diminuir a radioatividade no compartimento celular, nas prote?nas plasm?ticas e celulares. Na morfometria das hem?cias, apenas a hortel? foi capaz de alterar a forma e a rela??o per?metro/?reas das hem?cias. Na biodistribui??o do radiobiocomplexo pertecnetato de s?dio (Na99mTcO4) a hortel? aumentou a capta??o do Na99mTcO4 no rim, no ba?o, no f?gado e na tire?ide, enquanto que o hip?rico diminuiu a capta??o do Na99mTcO4 no osso, no est?mago, no pulm?o e na tire?ide, e aumentou no p?ncreas. Na sobreviv?ncia de culturas bacterianas o hip?rico foi capaz de proteger a bact?ria do efeito danoso do cloreto estanoso (SnCl2). O hip?rico n?o alterou a topologia nem protegeu o DNA plasmidial da a??o do SnCl2. Provavelmente os efeitos apresentados por ambos os extratos poderiam ser explicados por subst?ncias presentes nos extratos que poderiam alterar a morfologia das hem?cias, o transporte de ?ons pela membrana e/ou formar fitocomplexos. O estudo teve car?ter multidisciplinar com a participa??o das seguintes ?reas do conhecimento: Radiobiologia, Bot?nica, Endocrinologia, Fitoterapia e Hematologia