Thèse-Paris, 1914.

Thesis (M.S.)--University of Wisconsin--Madison, 1982. / Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1959. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (Ph. D.)--University of Wisconsin-Madison, 1974. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (PH. D)--University of Illinois, 1916. / "Reprinted from the American journal of botany 4 ... May, 1917." "Literature": p. 312-313.

No description available.

No description available.

Red radish (Raphanus sativus L.) anthocyanins were extracted from liquid nitrogen powdered epidermal tissue using acetone and chloroform and isolated using C-18 resin. Chemical structure and conformation of major pigments were elucidated by 1-D and 2-D NMR, Mass Spectroscopy, HPLC, and spectral analyses. Two novel di-acylated anthocyanins, pelargonidin 3-0-[2-0-(β-D-glucopyranosyl)-(6-0- trans-p-coumaroyl- β-D-glucopyranoside)]-5-0-(6-0-malonyl-β-D-glucopyranoside)and pelargonidin 3-0-[2-0-(β-D-glucopyranosyl)-(6-0-trans-feruloyl-β-D-glucopyranoside)]- 5-0-(6-0-malonyl-β-D-glucopyranoside) represented 70% of total pigment. Two mono-acylated anthocyanins (20%) were pelargonidin 3-0-[2-0-(β-D-glucopyranosyl)-(6-0-trans-p-coumaroyl-β-D-glucopyranoside)]-5-0-(β-D-glucopyranoside) and pelargonidin 3-0-[2-0-(β-D-glucopyranosyl)-(6-0-trans- feruloyl-β-D-glucopyranoside)]-5-0-(β-D-glucopyranoside). NOESY revealed folding of the molecule. Electrospray (ES-MS) and tandem mass spectroscopy (MS-MS) were tested as tools for anthocyanin characterization. Anthocyanins were semi-purified using C-18 resin, washed with acidified water and ethyl acetate, and recovered with acidified methanol. Samples were injected into a mass spectrometer in aqueous or methanolic solutions. Charged character of anthocyanins favored the fast and effective detection of intact molecular ions requiring minimal sample preparation and with little interference. MS-MS provided clear and characteristic fragmentation patterns. Qualitative and quantitative anthocyanin pigment content of radish cultivars grown at 2 locations (Corvallis and Hermiston, OR) and harvested at 2 maturity stages, were evaluated. Pigment content depended on cultivar, root weight and location, with higher amounts obtained at Hermiston. Spring cultivars (n=22) had pigmentation in the skin, ranging from 39.3 to 185 mg anthocyanin/100g skin. Red-fleshed Winter cultivars (n=5) possessed from 12.2 to 53 mg anthocyanin/100g root. The major pigments were pelargonidin-3-sophoroside-5-glucoside, mono- or di-acylated with cinnamic and malonic acids; individual proportions varied among cultivars. Estimated pigment yields ranged from 1.3 to 15.8 kg/ha. Acylated and non-aeylated pelargonidin were isolated using semi-preparative HPLC to evaluate the effect of glycosylation and acylation in spectral characteristics, molar absorptivity and color. Molar absorptivity ranged from 15,600 to 39,590 for pelargonidin-3-glucoside and pg-3-rutinoside-5-glucoside acylated with p-coumaric acid, respectively. An hypsochromic shift on λmax was observed in presence of glycosylation. Pelargonidin-3,5-diglucoside and 3,5-triglucoside showed yellow-orange hue angle (>40°) in pH 1.0 buffer, higher than other pg-derivatives. Cinnamic acid acylation caused a bathochromic shift. Malonic acid acylation had little effect on color and no effect on λmax. The solvent system affected molar absorptivity and visual color characteristic of the pigments. Acylation increased pigment resistance to acid hydrolysis. / Graduation date: 1999

Effects of acid rain on the dry weight, nitrogen and sulfur content of radish plants were determined. They were exposed to distilled water acidified with a 7:3 ratio of sulfuric acid and nitric acid and adjusted to pH levels 2.5, 3.0, 3.5, 4.0, 4.5, 5.0, and 5.7. A control group, exposed to distilled water only, was also used. Four hundred plants, divided in eight groups, were grown under laboratory conditions. At four, seven and ten week intervals, an equal number of plants of each pH level were removed from the soil.Plants exposed to water of lower pH values were found to be significantly higher (p < .05) in dry weight, percentage of nitrogen, and weight of sulfur per plant than crops at more neutral pH levels. The increased weight may be due to the greater availability of nitrates and sulfates at lower pH levels. These compounds probably contributed to better growth and greater dry weight of the plants at low pH levels.

Typescript. / Bibliography: leaves [94]-98. / [vi], 98 l graphs, tables