Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-02-01T21:57:42Z No. of bitstreams: 1 ConceicaoAparecidaDornelasMonteiroMaia_TESE.pdf: 4715408 bytes, checksum: ce943e481eedb60a4a5cb2e44f04c0f6 (MD5) / Approved for entry into archive by Elisangela Moura (lilaalves@gmail.com) on 2016-03-07T23:10:59Z (GMT) No. of bitstreams: 1 ConceicaoAparecidaDornelasMonteiroMaia_TESE.pdf: 4715408 bytes, checksum: ce943e481eedb60a4a5cb2e44f04c0f6 (MD5) / Made available in DSpace on 2016-03-07T23:10:59Z (GMT). No. of bitstreams: 1 ConceicaoAparecidaDornelasMonteiroMaia_TESE.pdf: 4715408 bytes, checksum: ce943e481eedb60a4a5cb2e44f04c0f6 (MD5) Previous issue date: 2014-08-22 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / O sistema RANK/RANKL/OPG contribui para a compreens?o do processo e regula??o da forma??o e reabsor??o do osso, tendo sido o maior avan?o na biologia ?ssea com rela??o a osteoclastog?nese. RANKL e OPG inibem RANK regulando a forma??o, ativa??o e sobreviv?ncia de osteoclastos na remodela??o ?ssea. O objetivo desse estudo foi investigar a express?o dos marcadores RANKL/RANK/OPG em dentes de ratos reimplantados, bem como observar a rela??o entre a express?o desses marcadores com o processo de reabsor??o dent?ria e ?ssea. Foram utilizados 30 incisivos superiores direitos de 30 ratos machos, adultos, da linhagem Wistar (Rattus norvegicus albinus). Os dentes foram avulsionados e divididos em dois grupos que permaneceram extra-alveolar em ar seco: G1 (n=15) - 5 minutos e G2 (n=15) - 60 minutos, e em seguida foram reimplantados e analisados nos intervalos de 1, 3 e 7 dias. Finalizado os per?odos experimentais, ocorreu a eutan?sia dos animais. Cortes longitudinais com 5?m de espessura foram obtidos e corados pela t?cnica H/E para a an?lise histol?gica e cortes com 3?m de espessura foram submetidos ? rea??o imuno-histoqu?mica mediante a utiliza??o de anticorpos prim?rios para ratos como OPG, RANK e RANKL. Os resultados demonstraram que o sistema RANK/RANKL/OPG participa ativamente, tanto do processo de reparo de dentes de ratos reimplantados, quanto das reabsor??es dent?rias e ?sseas; que RANKL apresentou maior imunomarca??o em ambos os grupos, participando em todas as fases da reabsor??o ?ssea e dent?ria; e o aumento da express?o de RANKL foi observada em ambos os grupos em todos os intervalos de tempo, comprovando que a resposta inflamat?ria no ligamento periodontal ocorre no in?cio do processo de reparo; e que a fraca express?o de RANK e OPG e o aumento da express?o de RANKL sugere uma diminui??o da reabsor??o ativa e aumento da reabsor??o reparada no osso e no cemento, sendo maior no osso. / The RANK / RANKL / OPG sy stem plays an important role in bone formation and resorption . This finding has been regarded as one of the m ost important advances in the understanding of bone biology with respect to osteoclastogenesis. The aim of this study was to investigate the expression of RANKL / RANK / OPG markers in reimplanted t eeth of rats, and to observe the relationship between the expression of these markers and to oth and bone resorption. Thirty male Wistar rats (Rattus norvegicus albinos) had their maxillary right incisors extrac ted , and were divided into 2 groups according to the period that the extracted teeth were kept in dry air before reimplantation : G1 (n = 15) - 5 minutes , and G2 (n = 15) - 60 minutes . After reimplantation, teeth were analyzed at intervals of 1, 3 and 7 da ys. After these experimental periods, the animals were euthanized. Longitudinal sections with 5?m thick were obtained and stained with Hematoxylin and Eosin for histological analysis , while 3?m thick sections were subjected to immunohistochemical analysis of OPG , RANK and RANKL. The results showed that the RANK / RANKL / OPG system actively participates in both the repair process, as well as tooth and bone resorption . Extr a - alveolar time of 60 minutes before replantation caused minor expressions of RANKL a nd OPG, not influencing the expression of RANK; RANKL immunostaining showed higher in both groups when compared to other biomarkers, participating in all phases of bone and tooth resorption; RANKL was associated to both osteoclastogenesis and c ell ular proliferation , and was expressed in both groups.