The purposes of this study were to determine if differences exist in distance-learning receptivity and perceived technology usefulness between American and Korean graduate students as well as Individualists and Collectivists. Results indicated that the two groups differed in distance-learning receptivity and perceived technology usefulness. However, cultural value tendency did not influence either receptivity or usefulness. Recommendations based on this study: 1. Researchers who are interested in cross-cultural field of distance learning should find what potential factors influence the differences in the receptivity and perceived usefulness between American and Korean group. 2. Administrators and decision makers who want to implement or adopt distance learning for their employees or students need to understand that cultural value, at least individualism and collectivism, is not a significant factor in distance learning. Instead, they should understand the importance of prior experience because people feel that distance is better than they??ve ever thought possible once they experience. 3. In implementing distance learning, practitioners should consider interactive media more than non-interactive media. Distance learning is mostly dependent upon technology. Practitioners should consider that distance-learning technology should be developed in terms of perceived usefulness to users. 4. In respect to usefulness, advanced and contemporary technologies were perceived more useful than traditional technologies in distance learning. Therefore, practitioners should also consider advanced technology rather than traditional technology in distance learning. Recommendations for future research: 1. It is suggested that Hofstede??s other cultural value dimensions should be included in future studies. 2. Future researchers should consider other factors such as personal background, learning style, skill level, and motivation. 3. Future research is needed to extend the current findings and test their generalizability to other types of users, for example, undergraduate students or organization employees. 4. This study used American and Korean samples only. Other national cultures should be tested with various cultural dimensions in a future study.

The purposes of this study were to determine if differences exist in distance-learning receptivity and perceived technology usefulness between American and Korean graduate students as well as Individualists and Collectivists. Results indicated that the two groups differed in distance-learning receptivity and perceived technology usefulness. However, cultural value tendency did not influence either receptivity or usefulness. Recommendations based on this study: 1. Researchers who are interested in cross-cultural field of distance learning should find what potential factors influence the differences in the receptivity and perceived usefulness between American and Korean group. 2. Administrators and decision makers who want to implement or adopt distance learning for their employees or students need to understand that cultural value, at least individualism and collectivism, is not a significant factor in distance learning. Instead, they should understand the importance of prior experience because people feel that distance is better than they??ve ever thought possible once they experience. 3. In implementing distance learning, practitioners should consider interactive media more than non-interactive media. Distance learning is mostly dependent upon technology. Practitioners should consider that distance-learning technology should be developed in terms of perceived usefulness to users. 4. In respect to usefulness, advanced and contemporary technologies were perceived more useful than traditional technologies in distance learning. Therefore, practitioners should also consider advanced technology rather than traditional technology in distance learning. Recommendations for future research: 1. It is suggested that Hofstede??s other cultural value dimensions should be included in future studies. 2. Future researchers should consider other factors such as personal background, learning style, skill level, and motivation. 3. Future research is needed to extend the current findings and test their generalizability to other types of users, for example, undergraduate students or organization employees. 4. This study used American and Korean samples only. Other national cultures should be tested with various cultural dimensions in a future study.

The endometrium is receptive to the embryo during a restricted window in the mid-secretory phase. My objectives were to develop a minimally-invasive endometrial sampling method for gene expression profiling, and to identify genes differentially expressed in the receptive phase. Twenty-three normo-ovulatory women underwent uterine fluid aspiration during the pre-receptive (LH+2) and receptive (LH+7) phase of the same natural cycle. RNA was extracted, reverse transcribed, amplified and hybridized to whole-genome microarrays. Unsupervised hierarchical clustering revealed self-segregation of pre-receptive and receptive samples. Importantly, profiling by uterine fluid aspiration was representative of biopsy. An unpaired t-test with a false discovery rate of 0.05 and a Δ threshold of 4-fold identified 245 unique transcripts as differentially expressed in the receptive phase. NanoString analysis validated 96% of these genes. This approach will now allow us to correlate expression of these candidate biomarkers to implantation outcomes, towards the development of clinical assays predictive for endometrial receptivity.

The endometrium is receptive to the embryo during a restricted window in the mid-secretory phase. My objectives were to develop a minimally-invasive endometrial sampling method for gene expression profiling, and to identify genes differentially expressed in the receptive phase. Twenty-three normo-ovulatory women underwent uterine fluid aspiration during the pre-receptive (LH+2) and receptive (LH+7) phase of the same natural cycle. RNA was extracted, reverse transcribed, amplified and hybridized to whole-genome microarrays. Unsupervised hierarchical clustering revealed self-segregation of pre-receptive and receptive samples. Importantly, profiling by uterine fluid aspiration was representative of biopsy. An unpaired t-test with a false discovery rate of 0.05 and a Δ threshold of 4-fold identified 245 unique transcripts as differentially expressed in the receptive phase. NanoString analysis validated 96% of these genes. This approach will now allow us to correlate expression of these candidate biomarkers to implantation outcomes, towards the development of clinical assays predictive for endometrial receptivity.

The research on transition in supersonic and hypersonic boundary layers has been reinvigorated in the last decades because of the increased interest in high-speed flight. The receptivity to environmental disturbances of high-speed boundary layers developing over flat plates or curved surfaces is a very important problem because the transition process is directly impacted by it. The main objective of the research is to determine the effect of small steps on laminar high-speed boundary-layers that are excited by freestream disturbances in the form of vorticity and acoustic waves. Both supesonic and hypersonic regimes are analyzed using a high-order compressible Navier-Stokes numerical algorithm. It is found that both the backward and the forward steps are capable of stabilizing the disturbances that propagate inside the boundary layer. This will potentially delay the formation of three-dimensional disturbances that are precursors to transition into turbulence.

Community college transfer pathways may play a critical role in realizing broadened participation in engineering; Community colleges serve as an important access-oriented pathway through the postsecondary system in the United States, and also depend on 4-year institutions to streamline vertical transfer. The extent to which 4-year institutions are receptive to community college transfer as a viable pathway to engineering degrees may play a significant role in its efficacy. This dissertation explores a few aspects of transfer receptivity at 4-year institutions to understand how they relate to the efficacy of vertical transfer pathways in engineering disciplines. The first manuscript is a case study of an articulation agreement partnership between one 4-year institution and two public community college partners. The second manuscript examines how transfer policies and institutional characteristics of 4-year institutions in the U.S. relate to the enrollment, graduation and reporting of transfer students in engineering. I use a combination of quantitative and qualitative methodologies across both manuscripts. The results of these studies revealed that: 1) specific challenges for transfer in engineering suggest that adequate examinations of transfer receptivity need be discipline-specific, 2) institutions encounter dissonance when simultaneously managing aims to increase access and prestige, 3) there is a need for shifts in policy and ranking systems that incentivize increases and improvements in vertical transfer, 4) there is a need for more transparency of transfer-related policies and transfer student data, and 5) our understanding of transfer matriculation remains well ahead of graduation outcomes. / Doctor of Philosophy / Community college transfer pathways may be critical to increasing the number of women and underrepresented minority engineers. Community colleges serve as an affordable way for students and families to begin their postsecondary education in the United States, but depend on universities to support community college transfer to complete bachelor's degrees. How well universities partner with community colleges in transfer may determine how well it works for students and families. This dissertation explores a few ways that universities encourage or discourage community college transfer as a way to earn a bachelor's degree in engineering. The first study looks closely at a transfer partnership between one university and two community colleges. The second study looks at how transfer policies and characteristics of universities relate to the enrollment, graduation and reporting of engineering transfer students. The results of these studies revealed several important themes. First, there are specific challenges to transfer in engineering. Next, institutions have a hard time increasing access to their institutions for community college students while also increasing their academic ranking and prestige. With this in mind, there is a need to shift policies and ranking systems that encourage institutions to focus more on increasing community college transfer. Next, universities need to be more transparent about their policies and data that influence transfer students. Finally, we know a lot more about how often transfer students successfully transfer to universities than we do about how many students end up graduating from the university and how long it takes them to do so.

The linear receptivity and stability of plane idealized detonation with one-step Arrhenius type reaction kinetics is explored in the case of three-dimensional perturbations to a Zel'dovich-von Neumann-Doering base flow. This is explored in both overdriven and explicitly Chapman-Jouguet detonation. Additionally, the use of a multi-domain spectral collocation method for solving the conventional stability problem is explored within the context of normal-mode detonation. An extension of the stability analysis to confined detonations in a slightly porous walled tube is also carried out. Finally, an asymptotic analysis of a detonation with two-step reaction kinetics in the limit of large activation energy and for general overdrive and reaction order is performed yielding a nonlinear evolution equation for perturbations that produce stable limit cycle solutions.

Em bovinos, o desenvolvimento embrionário pré implantacional depende das funções do endométrio bovino que tem suas funções mediadas por uma complexa interação da ação e dos efeitos dos hormônios esteroides ovarianos E2 e P4. Estes hormônios regulam a expressão gênica e controlam o ambiente uterino modulando, entre outros, a expressão de microRNAs e a rede de citocinas relacionadas ao sistema imune. Os objetivos do presente trabalho foram abordados em dois capítulos, sendo (I) comparar os efeitos dos distintos ambientes endócrinos peri-ovulatórios sobre a expressão de microRNAs (II) e na modulação do sistema IL1/TLR no endométrio bovino nos dias 4 e 7 após a indução da ovulação. Para isso, controlou-se farmacologicamente o crescimento do folículo objetivando induzir a ovulação de folículos de maior diâmetro (grupo folículo grande-CL grande, FG-CLG) ou de menor diâmetro (grupo folículo pequeno-CL Pequeno, FP-CLP). Vinte e duas vacas multíparas nelore, foram pré-sincronizadas, metade destes animais foram destinados para o grupo FG-CLG e receberam uma dose de prostaglandina F2&#945; (PGF) e um dispositivo de progesterona, juntamente com benzoato de estradiol no D10. No momento da retirada dos dispositivos de progesterona (entre D1,75 e D2,5) todos os animas receberam uma dose de PGF. A ovulação foi induzida com acetato de buserelina (D0). O que diferiu entre os tratamentos foi que os animais do grupo FP-CLP não receberam uma dose de PGF no D10 e o momento da retirada dos dispositivos foi entre D1,25 e o D1,5. No capítulo I, o a expressão de microRNAs foi determinada por qPCR nos dias 4 e 7. Dos 90 microRNAs testados, 21 apresentaram se up-regulated e dois down-regulated no grupo FG-CLG (P<0.1) no D4. No D7, quatro microRNAs foram diferentemente expressos, sendo um up-regulated e três down-regulated no grupo FG-CLG (P<0.1) no D7. Para os microRNAs diferentemente expressos determinou-se mRNA-alvos preditos. Uma análise de ontologia demonstrou que os mRNAs-alvos apresentaram enriquecimento funcional na via dos receptores de hormônios esteroides, entre outras. No capítulo II, o sistema IL1/TLR foi avaliado quanto a abundância de transcriptos envolvidos neste sistema, do microRNA bta-mir-155 e das proteínas IL1&#946; e IL1R1. A abundância relativa de mRNA apresentou diferença (P<0.1) na abundância dos mRNAs de IL1R1, TAB1 e FOXP3, das proteínas IL1&#946; e IL1R1, sendo essas moléculas up-regulated no grupo FG-CLG. O microRNA bta-mir-155 foi down-regulated no grupo FG-CLG (P<0.1). Diante disto, pode-se concluir que o ambiente endócrino peri-ovulatório determina o perfil de expressão de microRNAs e modula o sistema IL1/TLR no endométrio bovino / In cattle, the pre implantation embryo development depends on the functions of the bovine endometrium that has its functions mediated by a complex interaction of action and the effects of ovarian steroid hormones E2 and P4. These hormones regulate gene expression and control the modulating uterine environment among others, the expression of microRNAs and the network of cytokines related to the immune system. The objectives of this study were discussed in two chapters, (I) to compare the effects of different peri-ovulatory endocrine environment on the expression of microRNAs (II) and modulation of the IL-1 system / TLR in bovine endometrium on days 4 and 7 after induction of ovulation. For this, it was controlled pharmacologically follicle growth aiming to induce ovulation of follicles larger diameter (great grand-CL follicle group, FG-CLG) or smaller in diameter (small-CL Small follicle group, FP-PLC). Twenty two nelore multiparous cows were pre-sync, half of these animals were used for the FG-NCG group and received a dose of F2á prostaglandin (PGF) and progesterone device along with oestradiol benzoate in D-10. Upon withdrawal of progesterone devices (between 1.75 and D-D-2,5) all animas received a dose of PGF. Ovulation was induced with buserelin acetate (D0). What differed between treatments was that animals FP-CLP group did not receive a dose of PGF in the D-10 and the time of removal of the devices was between D-1,25 and D-1.5. In Chapter I, the expression of microRNAs was determined by qPCR on 4 and 7. Of the 90 microRNAs tested, 21 showed was up-regulated and down-regulated in two FG-CLG group (P <0.1) in the D4. In D7 four microRNAs were differently expressed, one up-regulated and down-regulated in three FG-CLG group (P <0.1) at D7. For differently expressed microRNAs was determined predicted mRNA-target. An ontology analysis showed that the mRNA-targets had functional enrichment in via the steroid hormone receptors, among others. In Chapter II, the IL-1 / TLR system was evaluated as the abundance of transcripts involved in this system, the bta-mir-155 microRNA and IL1&#946; and IL1R1 proteins. The relative abundance of mRNA was different (P <0.1) in the abundance of mRNAs IL1R1, TAB1 and FOXP3, the IL1&#946; and IL1R1 proteins, and these up-regulated molecules in the FG-CLG group. The bta-mir-155 microRNA was down-regulated in the FG-CLG group (P <0.1). Given this, we can conclude that the peri-ovulatory endocrine milieu determines the profile of microRNA expression and modulates the IL1 / TLR system in bovine endometrium

Em bovinos, o desenvolvimento embrionário pré implantacional depende das funções do endométrio bovino que tem suas funções mediadas por uma complexa interação da ação e dos efeitos dos hormônios esteroides ovarianos E2 e P4. Estes hormônios regulam a expressão gênica e controlam o ambiente uterino modulando, entre outros, a expressão de microRNAs e a rede de citocinas relacionadas ao sistema imune. Os objetivos do presente trabalho foram abordados em dois capítulos, sendo (I) comparar os efeitos dos distintos ambientes endócrinos peri-ovulatórios sobre a expressão de microRNAs (II) e na modulação do sistema IL1/TLR no endométrio bovino nos dias 4 e 7 após a indução da ovulação. Para isso, controlou-se farmacologicamente o crescimento do folículo objetivando induzir a ovulação de folículos de maior diâmetro (grupo folículo grande-CL grande, FG-CLG) ou de menor diâmetro (grupo folículo pequeno-CL Pequeno, FP-CLP). Vinte e duas vacas multíparas nelore, foram pré-sincronizadas, metade destes animais foram destinados para o grupo FG-CLG e receberam uma dose de prostaglandina F2&#945; (PGF) e um dispositivo de progesterona, juntamente com benzoato de estradiol no D10. No momento da retirada dos dispositivos de progesterona (entre D1,75 e D2,5) todos os animas receberam uma dose de PGF. A ovulação foi induzida com acetato de buserelina (D0). O que diferiu entre os tratamentos foi que os animais do grupo FP-CLP não receberam uma dose de PGF no D10 e o momento da retirada dos dispositivos foi entre D1,25 e o D1,5. No capítulo I, o a expressão de microRNAs foi determinada por qPCR nos dias 4 e 7. Dos 90 microRNAs testados, 21 apresentaram se up-regulated e dois down-regulated no grupo FG-CLG (P<0.1) no D4. No D7, quatro microRNAs foram diferentemente expressos, sendo um up-regulated e três down-regulated no grupo FG-CLG (P<0.1) no D7. Para os microRNAs diferentemente expressos determinou-se mRNA-alvos preditos. Uma análise de ontologia demonstrou que os mRNAs-alvos apresentaram enriquecimento funcional na via dos receptores de hormônios esteroides, entre outras. No capítulo II, o sistema IL1/TLR foi avaliado quanto a abundância de transcriptos envolvidos neste sistema, do microRNA bta-mir-155 e das proteínas IL1&#946; e IL1R1. A abundância relativa de mRNA apresentou diferença (P<0.1) na abundância dos mRNAs de IL1R1, TAB1 e FOXP3, das proteínas IL1&#946; e IL1R1, sendo essas moléculas up-regulated no grupo FG-CLG. O microRNA bta-mir-155 foi down-regulated no grupo FG-CLG (P<0.1). Diante disto, pode-se concluir que o ambiente endócrino peri-ovulatório determina o perfil de expressão de microRNAs e modula o sistema IL1/TLR no endométrio bovino / In cattle, the pre implantation embryo development depends on the functions of the bovine endometrium that has its functions mediated by a complex interaction of action and the effects of ovarian steroid hormones E2 and P4. These hormones regulate gene expression and control the modulating uterine environment among others, the expression of microRNAs and the network of cytokines related to the immune system. The objectives of this study were discussed in two chapters, (I) to compare the effects of different peri-ovulatory endocrine environment on the expression of microRNAs (II) and modulation of the IL-1 system / TLR in bovine endometrium on days 4 and 7 after induction of ovulation. For this, it was controlled pharmacologically follicle growth aiming to induce ovulation of follicles larger diameter (great grand-CL follicle group, FG-CLG) or smaller in diameter (small-CL Small follicle group, FP-PLC). Twenty two nelore multiparous cows were pre-sync, half of these animals were used for the FG-NCG group and received a dose of F2á prostaglandin (PGF) and progesterone device along with oestradiol benzoate in D-10. Upon withdrawal of progesterone devices (between 1.75 and D-D-2,5) all animas received a dose of PGF. Ovulation was induced with buserelin acetate (D0). What differed between treatments was that animals FP-CLP group did not receive a dose of PGF in the D-10 and the time of removal of the devices was between D-1,25 and D-1.5. In Chapter I, the expression of microRNAs was determined by qPCR on 4 and 7. Of the 90 microRNAs tested, 21 showed was up-regulated and down-regulated in two FG-CLG group (P <0.1) in the D4. In D7 four microRNAs were differently expressed, one up-regulated and down-regulated in three FG-CLG group (P <0.1) at D7. For differently expressed microRNAs was determined predicted mRNA-target. An ontology analysis showed that the mRNA-targets had functional enrichment in via the steroid hormone receptors, among others. In Chapter II, the IL-1 / TLR system was evaluated as the abundance of transcripts involved in this system, the bta-mir-155 microRNA and IL1&#946; and IL1R1 proteins. The relative abundance of mRNA was different (P <0.1) in the abundance of mRNAs IL1R1, TAB1 and FOXP3, the IL1&#946; and IL1R1 proteins, and these up-regulated molecules in the FG-CLG group. The bta-mir-155 microRNA was down-regulated in the FG-CLG group (P <0.1). Given this, we can conclude that the peri-ovulatory endocrine milieu determines the profile of microRNA expression and modulates the IL1 / TLR system in bovine endometrium

A thesis submitted to the Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, in fulfillment of the requirements for the degree of Doctor of Philosophy July, 2015 / Introduction: Anastrozole is clinically effective in ovulation induction, but it has not been well researched. The aim of this study was to determine the optimal dose for anastrozole as a superovulator and ascertain its effects on implantation in Wistar rats; also to determine its effects on uterine morphology during early pregnancy using light microscopy and scanning electron microscopy (SEM). [Abbreviated Abstract. Open document to view full version] / AC2016