Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2018-02-21T21:07:29Z No. of bitstreams: 1 MariliaDanielaFerreiraDeCarvalhoMoreira_TESE.pdf: 929428 bytes, checksum: 122ddbe6f0f95a006b02637a7a41c56e (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2018-02-22T20:36:24Z (GMT) No. of bitstreams: 1 MariliaDanielaFerreiraDeCarvalhoMoreira_TESE.pdf: 929428 bytes, checksum: 122ddbe6f0f95a006b02637a7a41c56e (MD5) / Made available in DSpace on 2018-02-22T20:36:24Z (GMT). No. of bitstreams: 1 MariliaDanielaFerreiraDeCarvalhoMoreira_TESE.pdf: 929428 bytes, checksum: 122ddbe6f0f95a006b02637a7a41c56e (MD5) Previous issue date: 2017-12-12 / Estudos sobre a influ?ncia do c?lon na regenera??o hep?tica s?o escassos. Alguns t?m demonstrado que ap?s a colectomia ocorre aumento da regenera??o e sua redu??o em outros. Diante da controv?rsia, este trabalho visou examinar se a colectomia associada ? hepatectomia 70% influencia par?metros de regenera??o do f?gado em ratos. Foram utilizados 18 ratos Wistar distribu?dos em 3 grupos (6 animais cada). No grupo I (sham) foi realizada uma laparotomia com leve manipula??o de al?as; no grupo II uma colectomia + hepatectomia 70%; e no grupo III apenas hepatectomia 70%. No 6? dia p?s-operat?rio foi colhido sangue por pun??o card?aca sob anestesia, seguido de eutan?sia. Realizadas dosagens s?ricas de alanina aminotransferase (ALT), aspartato amino transferase (AST), fosfatase alcalina (FA), albumina, fator de crescimento de hepat?citos (HGF) e fator de crescimento transformador-? (TGF-?). A regenera??o do f?gado foi calculada pela f?rmula: (raz?o do peso do f?gado por 100g do peso corporal no momento da eutan?sia/peso do f?gado no pr?-operat?rio projetado por 100g de peso corporal) ? 100. Os testes de an?lise de vari?ncia (ANOVA) e Tukey foram usados, com signific?ncia p<0,05. Resultados: Houve uma menor e significante magnitude dos n?veis de ALT e AST no grupo II comparados com o grupo III (p<0,001). A albuminemia mostrou n?veis significativamente mais elevados no grupo II que no III. Os n?veis de HGF (408?18,2 pg/mL) e TGF-? (3,8?0,3 ng/mL) no grupo II foram significativamente mais elevados que o HGF (360?58,6 pg/mL) e TGF-? (2,3?0,4 ng/mL) no grupo III, respectivamente (p<0,001). O percentual de regenera??o hep?tica foi significativamente mais elevado no grupo II, do que no grupo III (p=0,003). Em conclus?o, este estudo concluiu que a colectomia realizada simultaneamente ? hepatectomia 70% influenciou positivamente na regenera??o do f?gado em ratos. Pesquisas adicionais s?o necess?rias para revelar os mecanismos moleculares deste efeito e para caracterizar a influ?ncia do c?lon na fisiologia do f?gado. / Studies on the influence of the colon on liver regeneration are scarce. Some have shown that after colectomy there is an increase in regeneration and its reduction in others. Faced with the controversy, this work aimed to investigate whether colectomy simultaneously performed with 70% hepatectomy influences liver regeneration in rats. Eighteen Wistar rats were distributed in 3 groups, 6 animals each. In group I (sham) only laparotomy was performed with mild manipulation of boweld loops; in group II 70% hepatectomy and simultaneous colectomy; in group III only 70% hepatectomy. On the 6th postoperative day, blood was collected by cardiac puncture under anesthesia, and remaining liver was removed. Serum measures of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (FA), albumin, hepatocyte growth factor (HGF) and transforming growth factor-? (TGF-?) were performed. Liver regeneration was calculated by (liver weight per 100g of the body weight at sacrifice/preoperative projected liver weight per 100g of the body weight) ? 100. The Analyses of variance (ANOVA) and Tukey tests were used, with significance p<0.05. There was significant lower levels of ALT and AST in group II rats when compared to group III (p<0.001). Albuminemia showed significantly higher levels in group II rats than in III (p<0.01). Serum levels of HGF (408?18.2pg/mL) and TGF-? (3.8?0.3ng/mL) were significantly higher in group II rats than HGF (360?58.6pg/mL), TGF-? (2.3?0.4ng/mL) in group III, respectively. The percentage of liver regeneration was significantly higher in group II than in group III (p = 0.003). In conclusion, this study demonstrated that colectomy, performed simultaneously with 70% hepatectomy, positively influenced liver regeneration in rats. Further research is needed to reveal the molecular mechanisms of this effect and to characterize the effects of the colon on liver physiology.