The first objective was to estimate total internal fat in beef cattle based on a technique that measures kidney fat (uKFd) using real-time ultrasound (RTU). Data were obtained from 109 cattle from four studies, and animals were scanned 7 d preslaughter for uKFd and ultrasound backfat thickness. At slaughter carcass kidney fat depth (cKFd), KPH weight, and total internal fat were measured. The second objective was to characterize residual feed intake (RFI) in finishing cattle fed high grain diets and to examine the relationships with growth, ultrasound, carcass, non-carcass, and tenderness traits in two studies involving Santa Gertrudis (n = 114) steers, and Angus bulls (n = 16) and heifers (n = 16). In both experiments, RFI was calculated as the difference between actual DMI and predicted DMI. Results for the first objective indicated that RTU can be used to estimate cKFd, KPH weight and total internal fat (IFAT). Prediction equations developed to predict IFAT had R2 that ranged from 0.65 to 0.97 (P < 0.05). Results for the second objective indicate that RFI was not correlated with ADG, but was positively correlated with DMI and feed conversion ratio. Carcass 12th-rib fat depth was positively correlated with RFI in Santa Gertudis steers, such that steers with low RFI were leaner than steers with high RFI. Residual feed intake was not correlated with carcass or non-carcass composition traits in Angus bulls and heifers. Marbling and tenderness traits were not associated with RFI. Results from these studies indicate that we are able to measure IFAT with RTU, and that beef cattle producers can utilize RFI to identify animals that are more efficient with minimal impacts on growth, carcass composition and tenderness.

Residual feed intake (RFI) is one method to identify feed efficient animals; however, this method is costly and time consuming therefore, identifying an indirect measure of RFI is important. Evaluating the glucoregulatory mechanisms in cattle selected for divergent RFI may provide insight into metabolic processes involved in feed efficiency. This study evaluated the effect of a glucose (GLUC) challenge on efficient (LRFI) and inefficient (HRFI) tropically adapted bulls and heifers. Insulin (INS) secretion was determined by radioimmunoassay (RIA) and GLUC was determined by colorimetry. Insulinogenic index (IIND) was calculated as the ratio of INS to GLUC (I/G). Bonsmara heifers were evaluated in two experiments. Similar results were observed in both experiments. RFI affected (P < 0.05) INS response; with LRFI heifers having a greater INS response than HRFI heifers. Similarly, RFI affected (P < 0.05) IIND; with LRFI heifers having a greater IIND than HRFI heifers. In Santa Gertrudis bulls, RFI did not affect (P > 0.05) GLUC conc. or Ins. response; however, numerically HRFI bulls had a greater INS response than LRFI bulls. RFI affected (P < 0.05) IIND; with LRFI bulls having a lower IIND than HRFI bulls. In Brahman heifers (Exp 1), RFI did not affect (P > 0.05) GLUC concentration or INS. response; however, numerically HRFI heifers had a greater INS response than LRFI heifers. RFI affected (P < 0.05) IIND; with LRFI heifers having a lower IIND than HRFI heifers. In Brahman bulls (Exp 2), RFI affected (P > 0.05) INS response; with HRFI bulls having a greater INS response than LRFI bulls. RFI affected (P < 0.05) IIND; with LRFI bulls having a lower IIND than HRFI bulls. Bonsmara cattle evaluated for RFI had a response to an influx of exongenous glucose that was opposite to that observed in the Brahman and Santa Gertrudis cattle evaluated for RFI. Insulinogenic index was significantly different between RFI groups in each experiment. The lower amount of INS required for clearance of the GLUC from the circulation of the Brahman and Santa Gertrudis cattle fits with our hypothesis that more efficient cattle would require less INS than the less efficient cattle. Further research and studies need to establish glucoregulatory differences between breeds and sexes of cattle evaluated for RFI.

Objectives of this study were to characterize residual feed intake (RFI) in growing calves and to examine relationships with growth, carcass composition, physiological indicators, energy metabolism and reproduction. To accomplish these objectives, multiple experiments were conducted. In all experiments, RFI was calculated as the difference between actual DMI and expected DMI from linear regression of DMI on mid-test metabolic BW and ADG. To examine the relationships between RFI and rate and composition of growth, and reproduction an experiment was conducted with postweaning Brangus heifers (N = 348). Measures of carcass composition (longissimus muscle area, LMA; and 12th rib fat thickness, BF) were obtained by ultrasound at the start and end of each experiment. To determine if serum IGF-I concentration is associated with RFI, two experiments were conducted with Angus bulls and heifers (N = 95) divergently selected for serum IGF-I concentration. To evaluate relationships with energy metabolism, calves with low and high RFI were selected for determination of heart rate as an indicator trait for energy expenditure (4 experiments) and liver mitochondrial function (3 experiments). Residual feed intake was not correlated with ADG, but was positively correlated with gain in BF such that the more efficient calves were leaner. Calves from the low IGF-I selection line had lower RFI suggesting that RFI and IGF-I are related and that IGF-I could be used as an indicator trait to aid in selection for improved RFI in Angus cattle. Calves with low RFI had lower energy expenditure and greater mitochondrial acceptor control ratios than calves with high RFI suggesting improved regulation of energy metabolism. A similar percentage of heifers with low RFI attained puberty as those with high RFI by the end of the experiment. Results from this study indicate that producers can utilize RFI to select for improved feed efficiency with minimal impact on growth, carcass composition and reproduction.

Feed represents a large portion of input costs for dairy operations. Decreasing the feed consumed, measured here as dry matter intake (DMI), would therefore be advantageous to producers. However, this decrease could result in cows that do not supply the necessary energy to maintain high production, growth, and reproduction. Measures of feed efficiency try to capture important energy expenditures to ensure that cows consume less while maintaining production. Feed efficiency in this study is measured in residual feed intake (RFI), which is calculated as the difference between DMI and expected DMI based on production and growth measures. However, the RFI model does not capture the energy stores required for reproduction; therefore, selection for RFI may lead to reduced reproductive performance. In this thesis, I explored the phenotypic and genetic relationships between RFI, DMI, and reproductive performance as measured by number of services (NS), days open (DO), and days to first calving (DFC). First, ANOVA tests and phenotypic correlations determined the phenotypic relationship between the traits. Then, heritability and genetic correlations were used to estimate the genetic parameters. Phenotypically, results indicated mixed relationships between traits. Heritability estimates were low for reproductive traits and moderate for DMI and RFI. There was no unfavorable genetic relationship between DMI or RFI with any of the reproductive traits. Therefore, neither selection for DMI nor RFI will unfavorably impact reproduction. / Master of Science

Objectives of this study were 1) to characterize the relationship between beef cattle efficiency, namely residual feed intake (RFI), and digestive, microbial, and fermentation parameters in growing beef calves and 2) to examine the effects of added dietary hydrolysable or condensed tannin on animal performance and efficiency, fermentation and carcass and non-carcass traits. To accomplish the first objective, multiple RFI studies were conducted and in all studies RFI was calculated as the difference between actual and expected dry matter intake (DMI) based on average gaily gain (ADG) and body weight0.75(BW). A total of 187 head selected out of a population of 600 head of growing beef calves were evaluated for diet and nutrient digestibility, ruminal and fecal volatile fatty acids (VFA) concentrations and methane producing activity (MPA). Low RFI calves consumed less DMI and had lower feed conversion ratios (FCR) as compared to high RFI calves. Low RFI calves also had higher diet and nutrient digestibilities compared to high RFI calves. Residual feed intake was negatively correlated with diet and nutrient digestibilities such that more efficient animals had higher digestibilities. Low RFI calves tended to have lower ruminal propionate and higher acetate:propionate ratios when fed a high-forage diet. Calves with divergent RFI did not have different gross microbial populations as evidenced by the Firmicute:Bacteriodetes ratio, but low RFI calves tended to have higher fecal Prevotella spp. and lower fecal Spirochaetes and ruminal Cyanobacteria. The importance of these subtle shifts in microbial ecology is not evident at this time and more research is needed to fully elucidate the interaction of host and microbes to fully grasp the importance of minor microbial deviations. No differences in 3 h MPA were detected in low vs. high RFI calves but low RFI calves had higher fecal MPA when sampled at 24 h; however, calculated methane emissions were lower for low RFI calves. Tannin supplementation had no effect on animal performance and efficiency, ruminal fermentation VFA concentrations, MPA, or ammonia concentrations in finishing beef steers. There was also no detrimental effect of tannins on carcass traits; however, hydrolysable tannin supplementation resulted in increased empty rumen mass. Results from these studies indicate that diet and nutrient digestibility are affected by RFI such that more efficient calves had higher DMD, microbial ecology is responsive to RFI such that minor microbial shifts were observed, and tannin supplementation, at the current inclusion rate, had no effect on animal and carcass performance.

These studies were designed to evaluate the relationships between residual feed intake (RFI) and performance of growing heifers and Brahman cows. Residual feed intake was determined for 77 heifers of diverse breedtypes (Angus, Brahman, Hereford, Holstein, Jersey and F1 crosses) during both the pre- and post-pubertal periods. Heifers were individually fed and allowed ad libitum access to feed for 84 +/- 6 d during the prepubertal feeding trial and 90 +/- 4 d during the post-pubertal feeding trial. Brahmaninfluenced heifers had lower RFI than heifers without Brahman influence during both the pre-pubertal (P < 0.05) and post-pubertal (P < 0.0001) periods. Residual feed intake determined during the pre-pubertal period was only a moderate predictor (r = 0.48; P < 0.0001) of RFI determined during the post-pubertal period. Residual feed intake was determined for 38 Bonsmara heifers over a 70-d feeding period. Heifers were fed a high roughage diet at 2.65% of body weight (BW). Weekly blood serum samples were analyzed for progesterone concentration by radioimmunoassay (RIA) to determine puberty. There were no observed differences between efficient and inefficient heifers for performance traits, age at puberty or conception, or cumulative achievement of puberty and conception. The postpartum performance of Brahman primiparous (n=16) and multiparous (n=38) cows previously evaluated postweaning for RFI was investigated. Females were weighed and evaluated for body condition score (BCS) at 28-d intervals prior to the start of the 2008 calving season. Weekly weights and BCS were collected beginning 21 d after calving. Blood serum samples were also collected weekly for progesterone analysis by radioimmunoassay (RIA), non-esterified fatty acid (NEFA) analysis by enzymatic colorimetry, and insulin-like growth factor-I (IGF-I) analysis by RIA. Females were exposed to vasectomized marker bulls after calving to aid in estrus detection. Eight and ten d following observed estrus, females were evaluated using ultrasonography via rectal palpation to determine the presence of a corpus luteum (CL). Efficient cows exhibited estrus, developed functional corpora lutea, and exhibited estrus with CL formation earlier (P < 0.05) than inefficient cows. Furthermore, a greater percentage (P < 0.05) of efficient than inefficient cows were pregnant at the end of the breeding season.

The first objective of this study was to validate the feeding behavior measurements from a radio frequency electronic system (GrowSafe (TM) System Ltd., Airdrie, AB, Canada) and examine the software sensitivity to different parameter settings (MPS) to quantify feeding behavior traits. Data was continuously recorded 24 h per day using the GrowSafe™ system for 32 heifers over 81-d. Ten animals were randomly selected and evaluated over 6-d using time-lapse video recordings. Different parameter settings (MPS) from the electronic system (GrowSafe (TM)) used to record feeding behavior data, bunk visits (BV) frequency and BV duration, were compared with the observed (video) values. The second objective of this study was to quantify meal criterion; examine the associations between feeding behavior traits, performance, and feed efficiency; and the effects of breed type on feed efficiency (residual feed intake - RFI) and feeding behavior traits in heifers fed high-grain diets. Results from study one demonstrated that the GrowSafe (TM) system 4000E could accurately predict BV and meal data compared to observed data. The 100 s, used for the maximum duration between consecutive EID recordings to end an uninterrupted BV, was the appropriate MPS to predict BV frequency and duration, and meal frequency and duration compared to observed data using the GrowSafe (TM) 4000E system. The system’s ability to detect the animal’s presence or absence at the feed bunk was 86.4 and 99.6 percent, respectively. Results from the second study demonstrated that the meal criterion for heifers fed high-grain diets was 13.8 min. The 4 methods to calculate meal criterion demonstrated no differences in results of frequencies and durations of meal and the number of bunk visits per meal. Similar phenotypic correlations were found between the feeding behavior traits with RFI derived from the base model or with adjustments for final back fat. The adjustment of RFI to final back fat changed the RFI rank between breeds. The addition of feeding behavior traits to the RFI base model could accounted up to 40.4 percent of the variation in DMI not explained by ADG or MBW.

Cattle classified as having low residual feed intake (RFI) phenotypes are those that consume less feed than expected based on body weight and growth performance. Mechanisms contributing to the variation in RFI are not fully understood. Previous studies have shown that cattle of divergent RFI phenotypes have different levels of energy expenditures, which are associated with heat increment, basal metabolism, thermoregulation responses, and physical activity. The objectives of this experiment were to characterize residual feed intake (RFI) in growing heifers and to determine if variation in whole-animal energy expenditure contributes to differences in RFI. Brangus heifers (n =120) were individually fed a roughage-based diet (1.93 Mcal ME/kg DM) diet twice daily and feed refusals measured weekly. Heifers were weighed once weekly for 70 d and RFI calculated as the difference between actual and expected DMI from linear regression of DMI on ADG and mid-test BW0.75. Immediately following the 70 d study, oxygen pulse rate (mL O2/heart beat) and 48-h heart rates were measured on 8 high and 8 low RFI heifers to estimate energy expenditure. Daily heart rates and oxygen pulse rates were higher (P < 0.05) in heifers with high RFI compared to those with low RFI. As a result, energy expenditure (kcal/BW0.75) was estimated to be 17.4 percent greater (P < 0.05) in high-RFI heifers then low-RFI heifers. Mortality and morbidity losses caused by bovine respiratory disease (BRD) continue to negatively impact the net revenues of the beef cattle industry. Stress can predispose calves arriving at feedlots to BRD by impairing their immune system with calves having more excitable temperaments possibly having a greater risk. The objectives of the second study was to examine the effects of metaphylactic treatment and temperament on performance, feed intake, feed efficiency, and feeding behavior traits in steers. Santa Gertrudis steers (n =119) were weighed and randomly to control (CON; no antimicrobial treatment) or metaphylactic (MET; 1.5 mL/45 kg BW of ceftiofur crystalline free acid) treatments. Steers were weighed at 14-d intervals and individual intakes and feeding behavior traits measured using a GrowSafe sytems while fed a roughage-based diet (2.21 Mcal ME/kg DM). Objective (relative exit velocity; REV) and subjective (chute score; CS) measurements of temperament were measured on arrival and on day 28 of the study. Steers with higher REV weighed less, grew slower, consumed less feed, spent less time consuming feeding, had more feeding bouts per meal, had less backfat, smaller longissimus muscle area, and higher cortisol levels. Steers treated with MET had higher ADG than those receiving CON. Cattle with higher REV that received MET had less of a decrease in ADG, DMI, time spend consuming feed, and less of an increase in feeding bouts compared to high REV steers receiving CON. Results from this study suggest that process-control strategies, which quantify and manage inter-animal variation in calf temperament may facilitate more judicious use of antimicrobial products and provide more consistent and predictable responses to metaphylactic strategies.

Biological mechanisms that potentially contribute to residual feed intake (RFI) have not been fully understood in the grazing animal. The objective of this study was to determine the differences of RFI measured in confinement (RFIc) or grazing (RFIg) on animal performance. Animals were previously classified in confinement as high RFI (HRFIc), or low RFI (LRFIc) and subsequently under grazing as high (HRFIg) or low (LRFIg). Effects of forage quantity on dry matter intake (DMI), and biological mechanisms that contribute to variations in RFI were investigated using ultrasound, carcass traits and bacterial populations. Bulls were allotted to replicate bermudagrass pastures at low (LSTK) or high (HSTK) stocking intensities and heifers grazed one Ryegrass pasture. Ruminal microbial content was collected and profiled using bacterial tag-encoded FLX amplicon pyrosequencing technique. In 2009, bulls were harvested directly off the pasture and ultrasound and carcass measurements were determined. Data were analyzed using PROC GLIMMIX of SAS. Linear regressions were obtained using PROC REG to estimate RFI. In 2009, there was a difference for LRFIc bulls in F:G (P=0.032), and HRFIg bulls on LSTK had an interaction for ADG (P=0.043). HRFIg bulls had greater intakes regardless of STK (P=0.003). In 2010, HRFIc bulls remained heavier throughout with the greatest DMI (P=0.0095). There were no differences for any traits for 2010 RFIg bulls. At a LSTK, HRFIg bulls tended (P>0.05) to have a lighter gastrointestinal tract (GIT) weight (P=0.093) while liver weight (P=0.072) tended to be heavier for all bulls. The small intestine was heavier for LRFIg bulls (P=0.09) on a HSTK. There was an interaction for microbial bacteria identified in the rumen in 2009 on hemicellulolytic (P=0.048), starch (P=0.025), and pectinolytic (P=0.057) degrading bacteria. HRFIg bulls at a LSTK had a greater percentage for amylolytic and pectinolytic degrading bacteria (P=0.008 and P=0.051, respectively) in the large intestine. There were no interactions for any substrates in 2010. DMI (P<0.0001) was greater each year for HRFIc heifers and was greater (P=0.0168, P=<0.0001, P=<0.0001) each year respectively for LRFIg heifers. No differences were found for initial BW, final BW, MetBW, and ADG in the RFIc or RFIg classes. HRFIg bulls with the greatest forage availability consumed more and had heavier GIT, but it is still unclear how the microbial fauna affected the efficiency among RFI phenotyped bulls.

This study was conducted to: i) determine the effect of residual feed intake (RFI) on first parity fertility and subsequent lifetime productivity of 820 females ranked as low, medium, and high RFI in western Canada and ii) investigate the relationship between RFI measured as a heifer and re-measured as a mature cow. No significant correlations were found between RFI and fertility/productivity traits. A negative trend (P < 0.10) was observed between RFI, RFIfat and MPPAbw, however, this trend was no longer apparent when RFI was adjusted for back fat and feeding event frequency (RFIfat&activity). No significant (P < 0.05) relationship was found between heifer RFI and cow RFI. These results suggest that selection for feed efficient, low RFI heifers i) has no impact on their fertility and productivity as cows, and ii) may not lead to superior feed efficiency as a mature cow as measured by RFI and Cow DMI. / February 2017