The effects of intensity of the unconditioned stimulus (US) on the acquisition and extinction of the conditioned emotional response (CER) in rats were investigated. The US intensities studied were 0.28, 0.49, 0.85, 1.55, and 2.91 ma. Both acquisition and extinction of the CER were found to be monotonic functions of US intensity, with the higher US intensities producing more rapid acquisition and more resistance to extinction. The lowest shock intensity failed to produce suppression. The 0.49 ma. subjects typically showed a partial recovery of normal operant behavior after development of a fairly profound CER. The results were interpreted as consistent wth the supposition that the CER is acquired in accordance with Pavlovian laws of classical conditioning. / Thesis / Master of Arts (MA)

This study investigated the role of Sic particles in the mechanical behaviour of a metal matrix composite (Al-SiC). Measurements of the development and magnitude of internal stresses were performed from Bauschinger experiments in the aluminum matrix A-356 reinforced with the Sic particles. The behavior of the Al matrix itself was also analyzed. The level of internal stresses in the particulate reinforced composite was found to saturate after 0.9% plastic strain and after 1.3% in the unreinforced matrix. The initial development of the unrelaxed internal stresses was analyzed using both microscopic and macroscopic models of the load-bearing role of the Sic particles. The Sic particles were found to have little influence on the plastic flow of the composite beyond the initial plastic deformation as the size and distribution of the Sic is very non-uniform. The effect of the Sic phase was compared to continuous fibers embedded in a metallic matrix. A model system of pure copper reinforced with continuous tungsten fibers was used for this purpose. The effect of the internal stresses on the dimensional stability of the particulate reinforced and the unreinforced matrix was also investigated. The generation of dislocations arising from the thermal cycling of those materials was also analyzed by reference to the increase in flow stress observed after thermal cycling and from a model based on dislocations production due to the difference in coefficient of thermal expansion of the phases. / Thesis / Master of Engineering (ME)

This thesis is concerned with the effects of previous ex­perience with electric shock on the acquisition of a conditioned emotional response (CER) to a signal preceding shock. Rats with prior shock experience were slow to acquire the normal CER, which is manifested by a decrease in the rate of food-motivated lever pressing. However, this slow acquisition did not seem to result from adaptation or habituation to shock, as had previously been proposed. Rather, prior experience with unsignalled shock tended in itself to inhibit the rate of lever pressing. When later presented with a warning signal preceding shock, rats with prior shock ex­perience increased their rate of responding. This "dis-inhibition" persisted for several days, after which the usual decrease in rate occurred. / Thesis / Master of Arts (MA)

Falling weight deflectometer (FWD) is currently used by most highway agencies to determine the structural condition of the highway network. Utilizing the deflections measured by the FWD, the resilient moduli of layers in the flexible pavement is determined using backcalculation software packages. The moduli can be input into semi-empirical mechanistic equations to estimate the remaining life of the pavement system and aid in informing pavement engineers about timing of maintenance and rehabilitation needs. There have been concerns among practitioners and the research community about the adequacy of the resilient moduli determined by the backcalculation software. Some of the backcalculation models have been simplified and field verification may be needed. Field-measured stresses and strains may be used to quantify the reliability of the backcalculated moduli. The Virginia Smart Road, which has 12 different flexible pavement designs and was built and instrumented with pressure cells, strain gages, thermocouples, frost probes and moisture sensors. To validate the backcalculated moduli theoretically and through instrument response, this research was conducted with following objectives: 1) to determine the resilient moduli of the unbound granular materials on the Virginia Smart Road using small and large plates of the FWD; 2) to investigate the extent of spatial and temporal variability of the FWD deflections among pavement sections; 3) to develop a temperature correction model for the backcalculated HMA resilient moduli; 4) to define an appropriate backcalculation approach and compare the four widely used software approaches; and 5) to correlate backcalculated and laboratory measured moduli. In addition, the FWD measurements were used to establish a comparison between in-situ measured and computed stresses and strains in the pavement. The analytical approaches used are linear elastic, viscoelastic, and viscoelastic combined with nonlinearity. Results show that estimation of unbound granular materials moduli using surface deflections is more reliable when 457-mm-diameter loading plate is used. Analysis of deflections from different sensors showed evidence of spatial and temporal variability. The lowest coefficient of variation of deflections (7%) within sections occurred at low temperatures (2 to 6 °C), while the highest coefficient of variation (42%) occurred at temperatures between 35 to 40 °C. This resulted in the development of a deflection temperature correction model. The model was validated at different temperature ranges. A backcalculation procedure was defined to achieve good root mean square error using four selected software packages. This resulted in the selection of the most reliable software to perform moduli backcalculation. A correlation was established between the nonlinear models produced by backcalculation and laboratory testing of the granular 21-B material. However, for the HMA materials, difference in loading period between laboratory testing and FWD loading pulse could affect the results. The study found that when utilizing the backcalculated moduli, computed strains using viscoelastic modeling were comparable to in-situ measured values. Similarly, calculated stresses compared well with the field-measured stresses; especially at high temperatures. Mix properties, temperature of testing and loading were found to have an effect on the agreement between the measured and computed strains in the wearing surface. The study also recommended further validation of FWD measurements using embedded instruments to calibrate analytical models and further analysis of deflection data so that optimum number of testing points can be determined to limit amount of testing performed for determination of deflection variability. / Ph. D.

Biologically-mediated subsurface remediation by biofilm communities is a poorly understood process that is spatially and temporally dynamic. Two microbial responses, catabolism and the stress response glutathione-gated potassium efflux (GGKE), to benzene, pentachlorophenol (PCP), or Cd exposure were studied in up-flow sand columns to examine the contribution of each response to the overall functional response of a subsurface biofilm. Benzene was catabolized in the aerobic zone, and did not activate the GGKE response, and exhibited the highest biomass concentrations of all columns. PCP was not catabolized during this study, but was found to elicit two responses, oxidative phosphorylation uncoupling and GGKE, that appeared to be concentration dependent. Oxidative uncoupling was the controlling metabolic response up to 10 mg/L PCP, while the GGKE stress response was activated near 20 mg/L PCP. PCP column biomass did not show long-term biomass detachment, although immediate detachment occurred during initial GGKE activation. Cd column biomass activated the GGKE response as perturbing Cd concentrations increased. Extracellular polymeric substance (EPS)-Cd complexation was a possible detoxification mechanism, as biomass concentrations did not decrease with increasing Cd concentration, and increased as Cd concentrations decreased. Results of this study suggested that the increased exposure of electrophilic contaminants to sand column biomass did not cause biomass detachment. / Master of Science

Nitrification is a significant concern for drinking water systems employing chloramines for secondary disinfection. Utilities have implemented a range of disinfection strategies that have varying levels of effectiveness in the prevention and control of nitrification events, including optimizing the chlorine-to-ammonia ratio, maintaining chloramine residual throughout the distribution system, controlling pH, and temporal switching to free chlorination. Annual or semi-annual application of free chlorination is practiced by 23% of chloraminating systems on a temporary basis as a preventative measure, even though it has the undesirable consequences of temporarily increasing disinfection byproducts, facilitating coliform detachment, and altering water taste and odor. Although temporal free chlorination and other nitrification control methods have been widely studied in the field and in pilot-scale systems, very little is known about the stress responses of nitrifying bacteria to different disinfection strategies and the role physiological state plays in the resistance to disinfection. It is well known that many commonly studied bacteria, such as Escherichia coli, are able to better resist disinfection by free chlorine and chloramines under nutrient limitation through regulation of stress response genes that encode for DNA protection and enzymes that mediate reactive oxygen species. We compared the genomes of E. coli and the ammonia-oxidizing bacterium Nitrosomonas europaea, and found that many of the known stress response mechanisms and genes present in E. coli are absent in N. europaea or not controlled by the same mechanisms specific to bacterial growth state. These genetic differences present a general susceptibility of N. europaea to disinfection by chlorine compounds. Using an experimental approach, we tested the hypothesis that N. europaea does not develop increased resistance to free chlorine and monochloramine during starvation to the same degree as E. coli. In addition, N. europaea cells were challenged with sequential treatments of monochloramine and hypochlorous acid to mimic the disinfectant switch employed by drinking water utilities. Indicators of activity (specific nitrite generation rate) and viability (LIVE/DEAD® BacLight© membrane-integrity based assay) were measured to determine short-term effectiveness of disinfection and recovery of cells over a twelve day monitoring period. The results of disinfectant challenge experiments reinforce the hypothesis, indicating that the response of N. europaea to either disinfectant does not significantly change during the transition from exponential phase to stationary phase. Exponentially growing N. europaea cells showed greater susceptibility to hypochlorous acid and monochloramine than stationary phase E. coli cells, but had increased resistance compared with exponential phase E. coli cells. Following incubation with monochloramine, N. europaea showed increased sensitivity to subsequent treatment with hypochlorous acid. Complete loss of ammonia-oxidation activity was observed in cells immediately following treatment with hypochlorous acid, monochloramine, or a combination of both disinfectants. Replenishing ammonia and nutrients did not invoke recovery of cells, as detected in activity measurements during the twelve day monitoring period. The results provide evidence for the effectiveness of both free chlorine and chloramines in the inhibition of growth and ammonia-oxidation activity in N. europaea. Furthermore, comparison of viability and activity measurements suggest that the membrane integrity-based stain does not serve as a good indicator of activity. These insights into the responses of pure culture nitrifying bacteria to free chlorine and monochloramine could prove useful in designing disinfection strategies effective in the control of nitrification. / Master of Science

High-hostiles exhibit exaggerated physiological responses to stressors as seen by increased cardiovascular reactivity (BP & HR) (Rhodes, Harrison, & Demaree, 2002; Demaree & Harrison, 1997). This exaggerated physiological response style is associated with cardiovascular disease and premature death (Everson et al., 1997). This experiment hypothesized that diminished regulatory control would also be evident in the Acoustic Startle Response (ASR). In this experiment, high- and low-hostile undergraduate men (N = 40) were exposed to a series of startle probes before and after a cold pressor (CP). Startle responses were measured using electromyography (EMG) recorded over the orbicularis occuli. Cardiovascular measures of blood pressure and heart rate were also taken. A 2 X 2 mixed factorial ANOVA was performed with Group (high and low hostile) as the fixed factor, Condition (pre and post CP) as the repeated measure, and peak magnitude EMG (mV) of startle responses as the dependent variable. For startle responses, significant main effects for Group and Condition indicated that high hostiles had larger startle responses than low hostiles and startle responses decreased after the cold pressor (CP). A significant Group X Condition interaction effect was found. Post-hoc analyses revealed no significant group differences before the CP. After the CP, high-hostiles had significantly higher startle responses than-low hostiles. High-hostiles' startle responses did not change significantly after the CP, whereas low-hostiles' startle response magnitude decreased significantly after the CP. Low-hostiles HR increased significantly after the cold pressor and both groups SBP decreased significantly after the cold pressor. / Master of Science

Yes / Tumour hypoxia is associated with poor patient prognosis and therapy resistance. A unique transcriptional response is initiated by hypoxia which includes the rapid activation of numerous transcription factors in a background of reduced global transcription. Here, we show that the biological response to hypoxia includes the accumulation of R-loops and the induction of the RNA/DNA helicase SETX. In the absence of hypoxia-induced SETX, R-loop levels increase, DNA damage accumulates, and DNA replication rates decrease. Therefore, suggesting that, SETX plays a role in protecting cells from DNA damage induced during transcription in hypoxia. Importantly, we propose that the mechanism of SETX induction in hypoxia is reliant on the PERK/ATF4 arm of the unfolded protein response. These data not only highlight the unique cellular response to hypoxia, which includes both a replication stress-dependent DNA damage response and an unfolded protein response but uncover a novel link between these two distinct pathways. / SR, KBL, PV and MH were supported by a CRUK grant C5255/A23755 (awarded to E.M.H.). N.N. was supported by an MRC studentship (MC_ST_U16007). I. P.F. was supported by CRUK Oxford Centre Prize DPhil Studentship C38302/A12981. N.G. was supported by a Royal Society University Research fellowship. W.-C.C. was funded by CRUK grant 23969 (awarded to F.M.B.). S.F.E.-K. was supported by a Wellcome Trust Investigator Award (103844) and a Lister Institute of Preventative Medicine Fellowship (137661). J.G. was supported by a Jean Shanks Foundation/ Pathological Society of Great Britain and Ireland Clinical PhD Fellowship (JSPS CPhD 2018 01).

Personality tests are widely used in the field of Industrial/Organizational Psychology; however, few studies have focused on their psychometric properties using Item Response Theory. This paper uses IRT to examine the test information functions (TIFs) of two personality measures: the NEO-PI-R and scales from the International Personality Item Pool. Results showed that most scales for both measures provided relatively consistent levels of information and measurement precision across levels of theta (q). Although the NEO-PI-R provided overall higher levels of information and measurement precision, the IPIP scales provided greater efficiency in that they provided more precision per item. Both scales showed substantial decrease in precision and information when response scales were dichotomized away from the original 5 point likert scale format. Implications and further avenues for research are discussed. / Master of Science

The very high travel demands associated with hurricane evacuations require some strategies, such as contraflow sections, to be included in hurricane evacuation plans. However, the response or reaction of the evacuees to these strategies has not been given much attention in the past. This study concentrated on one particular strategy, contraflow segments, and investigated evacuees' willingness to use them through an animated survey. Usable data was collected from 821 respondents. The first part of the study dealt with six factors (service availability, police presence, exit location, entry congestion, availability of multiple entries, and limited choice) which were studied independently and compared against individual background characteristics. The distribution of the responses from the survey indicated that the presence of multiple entries or the availability of information about services increased the likelihood of evacuees switching to contraflow lanes, while the presence of police personnel for instance did not greatly alter the decision. Other factors like entry congestion or exits well before or well after initially desired ones decreased the willingness to use contraflow lanes. In the case where contraflow lanes were the only option on the main evacuation route (without the regular lane alternative), evacuees were willing to take detours to avoid the use of contraflow facilities. However, the effects of the above listed factors were associated with the background characteristics of the evacuees as the odds ratios in this study indicated. Previous contraflow or reverse lane experience for instance attenuated the effect of entry congestion on avoiding contraflow lanes. Contraflow experience on the other hand increased the likelihood of using the first entry when two entries were available and increased the willingness to switch to contraflow lanes when information about services was provided. Also, evacuation experience, presence of passengers affecting stops, and having dependents in the family improved the willingness to use contraflow lanes given information about services. Other characteristics like living in a hurricane prone area increased the inclination to use contraflow in the presence of police personnel and having passengers affecting destination choice increased the willingness to detour and avoid contraflow when regular lanes were not part of the main evacuation route from the respondent's origin. The second part of the study dealt with congestion and information about congestion levels along the regular and contraflow lanes. Different combinations of levels of congestion and information were presented to the respondents in the animated part of the survey. Respondents indicated their preference for contraflow or regular lanes in these scenarios. This data was used to develop a conditional logit model which predicted choice based on the presented options. Evacuees demonstrated an overall willingness to switch to contraflow lanes when these lanes were less congested than the regular lanes. However, with similar congestion levels on the regular and contraflow lanes, willingness to switch to contraflow lanes decreased as congestion levels increased. Information about upcoming congestion influenced evacuees' route choice decisions. Information motivated switching to contraflow lanes when conveying better downstream conditions along these lanes. Overall, evacuees demonstrated a willingness to benefit from any congestion improvement offered by contraflow lanes as opposed to assumptions in the literature claiming underutilization of these segments due to drivers' discomfort and unfamiliarity. / Master of Science / The very high travel demands associated with hurricane evacuations require some strategies, such as contraflow sections, to be included in hurricane evacuation plans. However, the response or reaction of the evacuees to these strategies has not been given much attention in the past. This study concentrated on one particular strategy, contraflow segments, and investigated evacuees’ willingness to use them through an animated survey. Usable data was collected from 821 respondents. The first part of the study dealt with six factors (service availability, police presence, exit location, entry congestion, availability of multiple entries, and limited choice) which were studied independently and compared against individual background characteristics. The distribution of the responses from the survey indicated that the presence of multiple entries or the availability of information about services increased the likelihood of evacuees switching to contraflow lanes, while the presence of police personnel for instance did not greatly alter the decision. Other factors like entry congestion or exits well before or well after initially desired ones decreased the willingness to use contraflow lanes. In the case where contraflow lanes were the only option on the main evacuation route (without the regular lane alternative), evacuees were willing to take detours to avoid the use of contraflow facilities. However, the effects of the above listed factors were associated with the background characteristics of the evacuees as the odds ratios in this study indicated. Previous contraflow or reverse lane experience for instance attenuated the effect of entry congestion on avoiding contraflow lanes. Contraflow experience on the other hand increased the likelihood of using the first entry when two entries were available and increased the willingness to switch to contraflow lanes when information about services was provided. Also, evacuation experience, presence of passengers affecting stops, and having dependents in the family improved the willingness to use contraflow lanes given information about services. Other characteristics like living in a hurricane prone area increased the inclination to use contraflow in the presence of police personnel and having passengers affecting destination choice increased the willingness to detour and avoid contraflow when regular lanes were not part of the main evacuation route from the respondent’s origin. The second part of the study dealt with congestion and information about congestion levels along the regular and contraflow lanes. Different combinations of levels of congestion and information were presented to the respondents in the animated part of the survey. Respondents indicated their preference for contraflow or regular lanes in these scenarios. This data was used to develop a conditional logit model which predicted choice based on the presented options. Evacuees demonstrated an overall willingness to switch to contraflow lanes when these lanes were less congested than the regular lanes. However, with similar congestion levels on the regular and contraflow lanes, willingness to switch to contraflow lanes decreased as congestion levels increased. Information about upcoming congestion influenced evacuees’ route choice decisions. Information motivated switching to contraflow lanes when conveying better downstream conditions along these lanes. Overall, evacuees demonstrated a willingness to benefit from any congestion improvement offered by contraflow lanes as opposed to assumptions in the literature claiming underutilization of these segments due to drivers’ discomfort and unfamiliarity.