Thesis (doctoral)--Uppsala University, 1983. / Includes bibliographical references (p. 37-48).

In this work we present the results of the analysis of the surface structures and absorption properties with respect to the CO and O2 molecules of the Sn/Rh and Rh/SnO2 model systems. In the part dedicated to the Sn structures on Rh surfaces with two different orientations - Rh(110) and Rh(111) - we have investigated the development of the core electron levels and valence band during the development of surface reconstructions and absorption of CO molecules. The surface reconstructions of the Sn/Rh(110) systems were studied for the first time. Difference in behaviour w.r.t. Sn/Rh(111) was observed and explanation offered. Finally, on in-situ prepared epitaxial SnO2 layers, the surface reconstruction (4×1) was observed. The CO adsorp- tion properties of Rh on polycrystalline and epitaxial SnO2 layers were also studied and difference in behaviour explained.

Made available in DSpace on 2010-04-20T20:15:15Z (GMT). No. of bitstreams: 0 Previous issue date: 1993-05-06T00:00:00Z / Mostra como a área de RH esta hoje configurada, particularmente através do estudo das mudanças em curso baseadas na utilização da informática pela mesma. Aborda as experiencias nacional e internacional, complementando-as com uma pesquisa de campo em sete empresas brasileiras. Identifica o novo papel emergente da área de RH, apontando tendencias a serem seguidas pelas áreas de RH das empresas brasileiras, referentes as suas políticas e ao uso da informática como instrumento de gestão de recursos humanos.

La place stratégique de la GRH dans l'amélioration des performances des entreprises est reconnue. Cette hypothèse est soutenue depuis deux décennies par la littérature appartenant au courant de Gestion Stratégique des Ressources Humaines (GSRH). Si la majorité des recherches consacrées à l'étude des liens entre la GRH et la performance ont abouti à des résultats significatifs, ces recherches sont toutefois restées muettes sur la résistance de ces liens dans le temps. Actuellement, l'inquiétude des entreprises est de savoir, compte tenu des risques encourus, comment maintenir le plus longtemps possible leur performance. En relevant cette problématique, l'objectif de notre recherche s'inscrit dans le souhait d'approfondir les travaux antérieurs et d'apporter un éclairage supplémentaire au courant de gestion stratégique des ressources humaines. Afin de répondre à notre problématique, nous avons mobilisé les fondements de l'approche "configurationnelle" des ressources humaines. Cette approche stipule qu'une stratégique RH composée de pratiques de GRH cohérentes en interne et en externe avec la stratégie d'affaires est susceptible d'assurer une performance de longue durée. Pour vérifier cette hypothèse, nous avons fait appel à des données longitudinales provenant de l'enquête REPONSE conduite par la DARES et l'institut BVA pour les années 1997-1998 et 2004-2005. Cette enquête a été menée sur un échantillon représentatif de 2978 établissements français de plus de 20 salariés en 1998, et de 2930 établissements également de plus de 20 salariés en 2005. Les principaux résultats de cette recherche, en statique et en dynamique, montrent que les associations simultanées entre les systèmes de GRH et les quatre dimensions de la performance (rentabilité, innovation, absentéisme et climat social) résistent à l'introduction du décalage temporel. Ce résultat soutient la pertinence de la cohérence de la stratégie RH comme un atout dans la maximisation de la performance durable. / For the two last decades, an abundant literature has been entirely devoted to the treatment of the place of the HRM in the implication of human resources for the achievement of strategic objectives and the improvement of the firms' performance. This literature concerns the Strategic Human Resources Management (SHRM). If the majority of the research carried out in the study of the bonds between the HRM and firms' effectiveness led to significant results, these studies remained silent on the question of resistance of this effect in a long time. Currently, firms are concerned about the way to maintain their performance as long as possible, taking into account the running risks. The sustainability of the performance thus becomes the managers' major concern. By raising this issue, our research aims to increase former studies and to provide a complementary light to the strategic human resources management. "Configurational" approach of human resources should be the conceptual foundation to find an answer to this issue. This approach stipulates that a strategy of human resources made up of internal and external coherent HRM practices with the strategy business, is likely to ensure a long term performance. Our method is based upon longitudinal data coming from REPONSE survey led by DARES and BVA Institute for the periods of 1997-1998 and 2004-2005. This survey was carried out into a representative sample of 2978 French Business Units of more than 20 employees in 1998, and 2930 Business Units of the same size in 2005. The principle static and dynamic results of this research show that simultaneous associations between HRM systems and the four dimensions of the performance (profitability, innovation, absenteeism and social climate) stand up to the introducion of temporal shift. This conclusion supports the relevance of human resource strategy's coherence like an asset in the maximization of sustainable performance.

Made available in DSpace on 2014-06-11T19:32:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-04-28Bitstream added on 2014-06-13T20:03:27Z : No. of bitstreams: 1 ianella_p_dr_sjrp.pdf: 5541345 bytes, checksum: a1c64b45b93d1e1381e39a7aadaede7d (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Nsf - Nacional Science Foundation / O cromossomo X apresenta conteúdo conservado entre as diferentes espécies de mamíferos. No de búfalo de rio (Bubalus bubalis), espécie que vem ganhando interesse econômico no Brasil e no mundo, sua morfologia é acrocêntrica. No presente trabalho, apresentamos o primeiro mapa RH do cromossomo X bubalino gerado a partir do recentemente construído painel de células híbridas irradiadas búfalo-roedor (BBURH5000). Este mapa contém um total de 33 marcadores derivados de bovino, incluindo dez genes, quatro ESTs e 19 microssatélites. Estes marcadores estão distribuídos em dois grupos de ligação: LG1 com oito marcadores e abrangendo 125.6 cR, e o LG2 com 25 marcadores abrangendo 366.3 cR. As freqüências de retenção (FR) dos marcadores variaram de 7,8% para o gene UREB1 a 28,9% para os microssatélites MAF45 e INRA30. O BBUXRH5000 foi comparado ao mapa de seqüência e mapa RH3000 do cromossomo X bovino evidenciando alguns poucos rearranjos entre as duas espécies, e alguns prováveis erros de mapeamento em uma das duas espécies quando comparado com BTAX build 3.1 bovino. A utilização de primers derivados de boi para mapeamento em búfalo foi realizada com êxito, e a distribuição dos marcadores ao longo do X considerada satisfatória, culminando em uma cobertura adequada para os primeiros esforços de mapeamento deste cromossomo. Análises comparativas do BBUX com o cromossomo X de outras espécies de mamíferos (humano, camundongo, ovelha, cavalo e cachorro) foram realizadas, revelando grande conservação de sintenia deste cromossomo na classe mamífera e, extensa conservação da ordem gênica entre búfalo e ovelha e búfalo e boi. O BBUXRH5000 aqui apresentado é um ponto de partida para a construção de mapas de alta resolução, necessários para caracterização de rearranjos que ocorreram durante a evolução e futuros estudos com o objetivo de dissecar características genéticas de interesse econômico. / The X chromosome shows conserved content among different mammalian species. In river buffalo (Bubalus bubalis), a brazilian and worldwide economic important specie, the X chromosome morphology is acrocentric. Here we report the first radiation hybrid map of the river buffalo X chromosome generated from a recently constructed river buffalo (Bubalus bubalis) whole-genome radiation hybrid panel (BBURH5000). This map contains a total of 33 cattle-derived markers, including ten genes, four ESTs and 19 microsatellites. The markers are distributed in two linkage groups: LG1 contains eight markers spanning 125.6 cR, and LG2 contains 25 markers spanning 366.3 cR. The retention frequency (RF) of individual markers across the panel ranged from 7.8% to the gene UREB1 and 28,9 to the microsatellites MAF45 and INRA30. The BBUXRH5000 was compared with the bovine sequence assembly (build 3.1) and RH3000 bovine X chromosome maps and showed few rearrangements between these species, and possible mapping errors in one of the two species when compared with BTAX build 3.1. The use of cattle-derived primers using carried out successfully and the markers distribution along the chromosome was satisfactory, resulting in adequate coverage for a first mapping effort of this chromosome. Comparative analysis between BBUX and X chromosome from other mammalian species (human, hamster, sheep, horse and dog) were carried out showed extensive sinteny conservation of the X chromosome in the Mammalian Class, and gene order conservation between river buffalo and sheep and river buffalo and cattle. The BBUXRH5000 here presented is the start-pointing for the construction of high-resolution map, which is necessary for characterization of rearrangements occurring during evolution and futures studies in order to dissect economically important traits.

Alloimmunisation becomes a problem when serological discrepancies occur in matching antigens between donors and patients for blood transfusion. The rate of alloimmunisation has been increased especially in multiply transfused patients. Blood group genotyping (BGG) is a DNA-based assay that aids in reducing this situation. Currently, many platforms of BGG have become available, in which every technique has its own advantages and disadvantages. All these platforms lack the ability to identify novel alleles that might have an unknown clinical significance. The advent of next-generation sequencing (NGS) offers identification of the unprecedented alleles due to its basis of sequence-based typing. Moreover, it provides an extreme high-throughput which may be able to screen many donors and patients in a single run. In this project, two approaches have been developed in generating sequencing libraries followed by sequencing on the Ion Torrent Personal Genome MachineTM platform (Ion PGMTM). The first approach was amplicon-based target selection using Ion AmpliseqTM Custom Panel, designated as Human Erythrocyte Antigens and Human Platelet Antigens Panel (HEA and HPA Panel). This panel assay screens 11 blood group systems, as well as 16 human platelet antigens. The outcome was extraordinary, in particular four novel alleles had been identified out of 28 samples, one in the RHCE gene 208C > T (Arg70Trp) in exon 2 and three in the KEL gene. The first SNP was 331G > A (Ala111Thr) in exon 4. The second SNP was 1907C > T (Ala636Val) in exon 17 and the third SNP was 2165T > C (Leu722Pro) in exon 19. However, some issues occurred regarding co-amplification of homologous genes. The second approach was a long-range polymerase chain reaction (LR-PCR) based approach. This method provided a high resolution assay by amplification of entire genes, including the non-coding area, of the Kell and Rh blood group systems. The Kell blood group was initially utilised as a model in order to apply the same approach on the Rh system. Most alleles encoding the antigens of the Kell blood group, especially the high prevalence ones, were identified. The Rh LR-PCR approach was carried out by amplification of the RHD and RHCE genes with seven amplicons. For five RhD-positive samples no mutations were observed within the coding areas. On the other hand, five serotyped weak D samples were genotyped as; two weak D type 1, two weak D type 2 and one DAR3.1 weak partial D 4.0 (RHD*DAR3.01). Regarding the RHCE, the following antigens (C, c, E, c) were predicted properly from the sequencing data. Moreover, the RHCE*ceVS.02 was identified. 64 and 39 intronic SNPs were identified in RHD and RHCE genes, respectively. The intronic SNPs assisted the genotyping process by identifying the haplotype of interest. Interestingly, the novel allele identified in the RHCE gene by the HEA and HPA Panel was confirmed to belong to the RHCE gene by the LR-PCR approach, indicating the panel misaligned it to the RHD gene. In conclusion, NGS paves the way to be an alternative substitution to the previous molecular techniques. It would supplant the conventional serology for typing blood for transfusion.

No description available.

The adsorption of oxygen on Rh(110) was investigated by thermal desorption spectroscopy (TDS), X-ray photoelectron spectroscopy (XPS) and scanning tunnelling microscopy (STM). The desorption of chemisorbed oxygen was observed at 700 K to 1200 K. Above 1400 K exists another desorption peak which is attributed to subsurface oxygen. The content of subsurface oxygen in Rh(110) influences the chemisorption states on the surface, so that the desorbing character of the surface oxygen species is changed. The subsurface-state needs high preparation temperature and has not any reaction with residual gas or added hydrogen in the chamber, which clarifies its spatial isolation. The XPS result showed that the binding energy of subsurface species is higher than the one of surface oxygen. This confirms the TDS observation. The subsurface-oxygen containing surface showed in STM oxygen induced hillock-like structures.

京都大学 / 0048 / 新制・課程博士 / 博士(工学) / 甲第18290号 / 工博第3882号 / 新制||工||1596(附属図書館) / 31148 / 京都大学大学院工学研究科高分子化学専攻 / (主査)教授 秋吉 一成, 教授 澤本 光男, 教授 松原 誠二郎 / 学位規則第4条第1項該当 / Doctor of Philosophy (Engineering) / Kyoto University / DFAM

京都大学 / 0048 / 新制・課程博士 / 博士(情報学) / 甲第18413号 / 情博第528号 / 新制||情||93(附属図書館) / 31271 / 京都大学大学院情報学研究科通信情報システム専攻 / (主査)教授 小野寺 秀俊, 教授 守倉 正博, 教授 佐藤 高史 / 学位規則第4条第1項該当 / Doctor of Informatics / Kyoto University / DFAM