Agriculture remains a dominant economic sector in many developing countries. Rice constitutes a staple food for more than half of the world's population and is the main meal of all Lao people (FAO, 2005). However, rice production faces many problems, including the effects of floods, drought and poor soil quality. These challenges combine to reduce rice productivity below what might be possible. However, before new agricultural policies are put in place, it is important to identify the factors associated with rice production efficiency. This thesis analyses technical and socio-economic factors that influence rice production and estimates the level of technical efficiency of individual rice farmers in Ban Home, Laos. / Survey data were collected in the region in 2003, for both wet season and dry season rice production. Using these data, a Cobb-Douglas frontier production function was estimated for each season using two approaches, deterministic and stochastic. During the wet season, several factors were statistically significant and positive in their effect on rice yield: area in rice production, level of fertilizer use, total labour, the use of a modern variety, sandy soil and contact with a professional agricultural advisor. During the dry season, only area in rice production and fertilizer were significant. Based on the frontier production functions, it is possible to conclude that the average technical efficiency of farmers is higher during the dry season than the wet season, for both the deterministic and stochastic approaches. / However, socio-economic factors were unable to explain the level of technical efficiency among farmers, when evaluated using a standard regression approach. By using a simple t-test to compare the mean level of efficiency of different groups of farmers, some significant differences emerged. Farmers who used credit were found to be more efficient than those who did not. Moreover, experienced farmers were more efficient than less experienced farmers. Also, farmers with less than 7 years of education were more efficient than more educated farmers.

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Flooding cycle in wetland rice (Oryza sativa L.) production systems is often subject to seasonal and cultural variations which may affect the availability and uptake of nitrogen in different ways. These factors may more or less influence the physiological and growth responses of the plant. In an effort to improve productivity in rice cropping systems, two controlled environment studies and a field trial were conducted to evaluate the growth and yield responses of rice to different flooding regimes and nitrogen fertilizer management strategies. In the first glasshouse trial, an upland cultivar (GM-1) was used to study the effects of four flooding regimes and three nitrogen application rates on the tillering, yield components and grain yield of rice. The field study determined the applicability of the results of the glasshouse trial to out-door environmental conditions, with the aim of gaining further insight into the impact of nitrogen application strategy on tiller and grain qualities. Two wetland cultivars (FKR-19 and N-19) and GM-1 were used to evaluate the effects of two flooding regimes and two nitrogen topdressing patterns. The second glasshouse trial determined the effect of hydro-priming on the establishment of direct seeded rice, and the effect of flooding on aerenchyma formation in rice roots. Results of the studies showed that flooding with standing water of 5 cm above the soil surface, irrespective of when it occurred, and nitrogen application increased the number of tillers and panicles, above ground dry matter, nitrogen uptake and grain yield of rice. However, late flooding and high nitrogen application rate of 220 kg ha-1 were found to encourage the production of late tillers, thereby reducing the efficiency of nitrogen use for grain production. Nitrogen application in three split doses tended to increase plant nitrogen content at heading; it slightly increased the protein content of the grains at maturity, but reduced the amylose content of the starch granules. Nitrogen application in two split doses led to increased grain yield in non-flooded plants, while the three-split treatment increased nitrogen uptake and grain yield in the flooded plants. Flooding significantly increased aerenchyma formation in the cortical tissues of rice roots, particularly at 50 mm behind the root tips. Hydro-priming seeds for 48 h improved plant establishment by shortening the germination and emergence times, and increasing the height and dry matter accumulation of seedlings, thereby ameliorating the susceptibility of rice to flooding stress. Overall, the thesis affirmed that controlled flooding is beneficial to rice production since it enhanced the growth and yield of the plant. It further revealed that early flooding and appropriate timing of moderate nitrogen application can ensure the conservation of water and nitrogen resources, including the quality of the environment, with no significant consequence for yield and productivity of the crop. / Thesis (M.Sc.Agric.)-University of KwaZulu-Natal, Pietermaritzburg, 2010.

Rice (Oryza sativa L.) is the second most important cereal crop in Malawi. Rice productivity in the dominant (85%) rainfed ecosystem is very low, with mean yield of only 1.4 t ha-1. Farmers plant traditional landraces that have low yield potential and are susceptible to various stresses such as drought and diseases. Development and introduction of improved rice varieties, with stress tolerance traits from introduced varieties, such as the New Rice for Africa (NERICAs), could significantly increase productivity. Previous attempts to introduce high yielding irrigated varieties into the dominant rainfed ecosystem in Malawi have not been successful because farmers basically did not adopt the varieties, claiming that the varieties were lacking in grain traits that they preferred but that the traits were present in their landraces. The notable traits mentioned, through previous informal surveys, were long grains, medium to slender shape and aromatic grain with intermediate gelatinization temperature. No formal study has been conducted to ascertain the preferences, and the genetic control of the traits, including yield and yield related traits, have not been studied. The objectives of this study were to: 1) confirm farmers’ preferences for grain traits using participatory rural appraisal; 2) determine amount of genetic variability for yield and yield related traits in Malawi rice landraces, 3) determine the genetic control and correlations of grain length, grain shape and 1000-grain weight, 4) determine the inheritance of aroma and gelatinization temperature and, 5) determine the genetic control, correlations and path coefficients of yield and yield related traits, in F2 generations of Malawi rice landraces and NERICA varieties crosses. To confirm the farmers’ preferences for grain traits, a participatory rural appraisal was conducted in 2006 in two villages that were representative of rainfed rice growing areas in Malawi. The villages were Liundi and Nawanga in Machinga and Salima Districts, respectively. Qualitative and quantitative data were collected through questionnaires and discussions with 190 respondents, as well as through observations. To determine variability among Malawi rice landraces, 19 landraces were planted at Lifuwu in a Randomized Complete Block Design with three replicates in 2006. Data on plant height, days to 50% flowering, number of panicles per hill, panicle length, number of filled grains per panicle, 1000-grain weight, panicle weight, grain length and grain yield were collected and analyzed. Four Malawi rice landraces were crossed to four NERICA varieties in 2006 in a North Carolina Design II mating scheme to determine the genetic control of grain size. F1 plants were raised in 2007 and in 2008, 16 F2 populations together with their parents were planted in a Randomized Complete Block Design with three replicates at Lifuwu. Data on grain length, grain shape and 1000-grain weight were collected and analyzed. To determine the inheritance of aroma and gelatinization temperature, four Malawi rice landraces were crossed to four NERICA varieties in 2006 and F1 plants were raised in 2007. In 2008, 16 F2 populations together with their parents were planted in a Randomized Complete Block Design with three replicates at Lifuwu. Aroma and gelatinization temperature were evaluated. To determine the gene action of yield and yield related traits, four Malawi rice landraces were crossed to four NERICA varieties in 2006 in a North Carolina Design II mating scheme and F1 plants were raised in 2007. In 2008, 16 F2 populations together with their parents were planted in a Randomized Complete Block Design with three replicates at Lifuwu. Data on grain yield, the number of panicles per hill, days to 50% flowering, panicle length, panicle weight and 1000-grain weight were collected and analyzed The participatory rural appraisal confirmed that that long, slender or medium shape grains, with aroma and intermediate gelatinization temperature were the key traits preferred by farmers. Therefore the farmer preferred traits of long, slender grains, with aroma and medium gelatinization temperature, must be selected for in any high yielding varieties to be developed for the rainfed rice ecosystem. Results showed that differences were significant (P=0.05) for all the traits that were studied. Heritability estimates were low to moderate: 18.3% for panicle weight, 40.0% for panicles per hill and 56.3% for days to 50% flowering date. The high genetic variability among the landraces could be used in a breeding programme to develop improved varieties for various morphological traits. The number of panicles per hill and 1000-grain weight combined moderate heritabilities with relatively high genetic advance and therefore could be reliable traits for yield improvement. Genetic analysis of grain size showed that Malawi rice landraces were variable for all three grain size characteristics, namely grain length, grain shape and 1000-grain weight. NERICA varieties were variable for 1000-grain weight. Sixteen F2 progenies were variable for all three characteristics, and the variability was significant (P=0.05). Heritability estimates were high (45.4%) for grain length and low for grain shape (12.3%) and for 1000-grain weight (14.3%) suggesting that early generation selection would be effective for grain length. Predominance of additive gene action for grain length and grain shape suggested that early generation selection would be effective for these traits. Selection for 1000-grain weight would be more effective in later generations because of preponderance of non-additive gene action in the control of this trait. The correlation between grain length and grain shape was positive (r=0.769) and highly significant (P=0.01) suggesting that breeders would choose to select for both traits simultaneously, or they would choose one of the traits to develop varieties with long grains and medium shape. Crosses between aromatic and non-aromatic varieties had non-aromatic F1. The F2 progenies segregated into 3:1 ratio for non-aromatic: aromatic suggesting that, in the Malawi rice landraces, aroma was probably simply inherited through a single recessive gene. F1 progenies, between parents with high and intermediate gelatinization temperatures had intermediate gelatinization temperature. F2 progenies segregated into 1:3 ratios for high; intermediate gelatinization temperature in three out of four crosses suggesting control by one dominant gene. The segregation pattern in one cross was not significantly different from 3:13 ratio for high: intermediate suggesting that two dominant genes, one an inhibitor, were controlling the trait. Breeding and selecting for aroma and intermediate gelatinization temperature could be accomplished relatively easily because the traits are simply inherited. The genetic variability for yield and yield related traits was wide and significant (P=0.05) in the F2 populations of Malawi rice landraces and NERICA varieties crosses indicating that the populations would be valuable sources to develop varieties with improved yield. Panicle weight and the number of panicles per hill were positively correlated with, and had high direct effects on grain yield, therefore they could be used to indirectly select for high yield. Grain yield, the number of panicles per hill and plant height were predominantly controlled by additive gene action suggesting that bulk breeding methods would be adopted for these traits. The days to 50% flowering, panicle weight and 1000-grain weight were predominantly under the control of nonadditive gene action suggesting that hybrid development would be profitable for these traits. Faya Mpata, Faya Zidyana and NERICA 3 could be the best parents for improving yield and yield related traits because they had high general combining abilities for the traits. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2010. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2010.

Grain yield of rice can be enhanced by breeding for drought tolerance and optimum shattering. New generations of rice, the interspecific fixed lines known to carry more drought tolerance traits, are a potential source for improvement of the Ugandan landraces but they frequently carry genes for grain shattering. Information gathered through surveys to understand rice production constraints and farmers’ preferred rice traits are limited and they are generally unranked. The objectives of this study were seven-fold. They were to 1) determine farmers’ perceptions on the key production constraints and preferences for rice in Uganda, 2) develop and appraise cheap and efficient rice breeding equipment, 3) determine anthesis and the grain shattering pattern of rice, 4) characterize for drought tolerance and grain-shattering ability in rice populations in Uganda, 5) investigate crossability between O. sativa and the new interspecific fixed lines, and 6 and 7) determine the inheritance and gene action controlling drought tolerance and grain shattering traits. The experiments were conducted at Namulonge, Uganda, from 2006 to 2009. Data on farmer’s perceptions of the key production constraints and preferences for rice in Uganda were obtained through participatory rural appraisal (PRA) methods and a formal household survey of 380 farmers from three regions in Uganda during March 2007 to June 2008. The results showed that the variety NERICA 4 was the most preferred by farmers. Rice yellow mottle virus disease, rice blast and African rice gall midge were major constraints under lowland conditions, while drought was the major constraint under upland conditions. The preferred traits in traditional varieties included aroma characteristics, grain size and the texture of cooked grains. Cultivars that combine high yield potential, early maturity, drought tolerance, aroma characteristics and special market attributes should be included in the breeding objectives. A new anther suction device that uses an ordinary household vacuum cleaner was fabricated. Its overall units include a pipette tip, silicone tubing, hose tubes, a metallic stand, a rubber stopper and a household vacuum cleaner of 1600 w capacity. It is efficient, cheaper, less bulky and has lower replacement costs than the commercial one. In addition, a hand-held single grain tester, which is efficient, cheaper and more suitable for field studies than the laboratory tester, was developed and appraised. Studies to determine the grain-shattering pattern in rice were conducted by making assessments at 10, 20 and 30 days after anthesis. The results revealed that the highest level of discrimination among the varieties was between 10 days and 20 days after anthesis. Among the genotypes tested, NERICA rice attained anthesis earlier than CT rice and O. sativa attained anthesis later than both rice groups. Appropriate methods of planting the parental lines were proposed for pollination to be synchronised. Interspecific rice and O. sativa were characterized in the field for drought tolerance and grain-shattering ability. There was high variability wihin species in grain shattering and drought tolerance traits, suggesting that selection could be effective. The distribution of grain shattering scores was not normal, suggesting that some selection had taken place for the trait, unlike the drought tolerance traits and leaf roll scores where normal distributions were observed. Results of the crossability test between O. sativa and interspecific fixed lines revealed that some genotypes had high crossability when used as males, or as females, or as both females and males. The best male genotype combiners were CT 16334(2)-CA-2-M, WAB 365-B-1H1-HB and IRAT 257, while the best females combiners were CT 16334(2)-CA-11-M, CT 16317-CA-4-M and IRAT 325. In addition, additive effects were more important that non-additive effects for pollen viability and seed set in the crosses. Genotypes with acceptable crossability and varying levels of drought tolerance and grainshattering ability were selected for further studies. The selected genotypes were crossed in a North Carolina II mating scheme, and as pairs of crosses, and used to understand the genetic mechanisms of drought tolerance at the reproductive and vegetative stages. Evaluation of the populations revealed that additive effects for filled grains were more important under drought stress and non-drought stress conditions than the non-additive effects. There were high heritability estimates for leaf roll under drought stress, implying that additive effects were more important. There was a higher contribution of additive effects for grain-shattering ability than nonadditive effects in the populations. Allelic tests for the shattering trait revealed that a single gene was in 46% of the crosses; two genes in another 46% of the crosses had duplicate dominant effects as well as complimentary dominant/recessive effects; and there were three genes in four percent of the crosses. Segregation ratios of 3:1 and 1:3 were also found in two crosses. This could be due to a reversal of the function of the genes. Another surprising finding was that the segregation ratio of 3:1 was found in a cross with loose grain attachment to the pedicel in an allelism test. This segregation was probably due to unfixed loci in some of the interspecific lines. This study identified rice breeding priorities for Uganda, prepared breeding tools and provided relevant information for generating lines that have improved drought tolerance and optimum grain-shattering ability by determining the modes of gene action for these two traits. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2010.

Rice (Oryza sativa L.) is the principal crop of North Eastern Tanzania but production is threatened by salt affected soils, drought and the use of un-adapted cultivars, among other constraints. Little research and hardly any breeding have been done on the aspects of salt tolerance of the crop in sub Saharan Africa, leading to low yields and low production in rice irrigation schemes under arid and semi arid conditions. A project was therefore implemented in North Eastern Tanzania during 2007-2010 seasons to investigate the possible breeding contributions to enhance productivity and production of the crop in salt affected areas. The objective of this study was to: a) determine farmers’ perceptions on both salt problems and their effects on rice crop productivity as well as establishing farmers’ needs and preferences for rice varieties in the targeted irrigated environment; b) determine the extent of salt problem in both soil and irrigation water in the available rice irrigation schemes in the North-eastern Tanzania; c) identify the major physiological mechanisms associated with salt tolerance in farmer-preferred native varieties and landraces; and d) determine the mode of inheritance of salt tolerance in rice. Participatory rural appraisal was conducted in Mkomazi and Mombo villages in Tanga region with the aim of understanding characteristics of rice–based farm economy, farmers’ perception of agriculture constraints and variety preferences in salt affected areas of North-eastern Tanzania. This was followed by a preliminary study to understand soil characteristics in relation to salt problems and its extent in selected nine rice irrigation schemes. Studies was established under controlled conditions to assess the salt tolerance of some rice farmers preferred rice cultivar and evaluate the putative traits in the rice materials that contribute to the performance of a genotype under saline and saline-sodic condition. Thereafter, genetic mechanism governing various morpho-physiological parameters in selected Tanzania local farmers’ preferred varieties and salt tolerant donors under saline and sodic soil conditions of North Eastern Tanzania were determined. A participatory rural appraisal (PRA) established that rice was a major staple food and cash crop and rice farming was a major economic activity in the area. However, soil degradation through increased salt affected soils was identified as the major factor responsible for irrigated rice yield decline. Major varieties grown are salt sensitive, and salt tolerant varieties were not available. The study also revealed that most farmers’ preferred traits of rice cultivars were high yield potential, aroma, early maturing, medium plant stature, tolerance to salt and drought. Improvement of these characters in new salt tolerant varieties would increase food production in fields with low or zero productivity and the well-being of the poor farmers. A soil characterisation study indicated the magnitude of the problem, whereby, seven out of nine studied irrigation schemes were affected and sodic and saline-sodic conditions were the dominant types of soils. Poor irrigation canals and management of irrigation water were the driving factors that contributed to salts accumulation causing a decline in productivity. Experiments were established under controlled environments to evaluate the tolerance of 10 and 11 rice genotypes under saline and saline-sodic stresses, respectively. Significant variation between genotype and significant interactions between genotype and salt treatment (P<0.001) were observed for all characters studied. Genotype Pokkalli, IR 67076-2B-21-2 and IR 56 showed superior performance under saline, whereas CSR 27, Nerica 2 and IR 56 had superior performance under saline-sodic. The study therefore established that, all the local farmer preferred cultivars except IR 56 performed poorly under both salt stress environments. High seedling vigour, less leaf injury, less Na+ and high K+ accumulation in leaves, low Na+/K+ ratio of ion uptake, high spikelet fertility, increased grains per panicle and 1000 grain weight were considered as the desirable characteristics therefore can be used in developing lines for salt tolerance for production under saline and saline - sodic conditions. Gene action and combining ability studies for nine morpho-physiological traits were studied under normal, saline and sodic soil environments. The rice populations were generated through 7 x 7 full diallel crosses and advanced to F2. The parents comprised two donors for saline tolerance, one donor for sodic tolerance and four salt sensitive farmers preferred varieties. Both additive and non-additive gene effects were important in the inheritance of the characters studied in all soil environments. However, additive effects were more important for the number of tillers, shoot Na+, Na+/K+ ratio and plant height. Both additive and non-additive gene effects were important for spikelet fertility, days to 50% flowering, number of grains per panicle, 1000 grain weight, and grain yield; however the magnitude of additive gene effect was higher than non additive effects. Amongst the parental lines, the best general combiners for yield along with other traits were TXD 306 and IR 67076-2B-21-2 under normal non-saline/sodic condition; IR 56, Pokalli and TXD 306 under saline condition and CSR 27 and TXD 306 under sodic conditions. The overall results from this study indicated the possibility of improving both yield and salt tolerance from this set of germplasm; therefore contributing to increasing rice yields in the marginal salt affected environments. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2010.

Rice is an important crop ranking third after maize and wheat in Kenya. Its demand is growing at 12% per annum, while production has stagnated for quite a number of years. This situation has lead to consumption outstripping production by about 84%. There is therefore an urgent need to step up domestic production. To understand farmers’ preferences, the first survey on rice production was carried out. To supplement on this survey information, focus group discussions and key informants were incorporated to further shed more information. The farmers demonstrated their preferences for varieties that were high yielding, hybrid rice and with high nutrient use efficiency and these constituted 53.7% of their wishes. Among the crops grown by the selected farmers, rice was the most important followed by maize, however, the hectarage of maize was higher followed by rice. The farmers also identified labour costs as a factor limiting production especially for irrigated rice and thus they preferred upland rice due to its ease of production. Farmers identified land preparation, inputs, planting, weeding and harvesting as the most expensive activities. The existing varieties were viewed as being highly dependent on inputs which they could not afford. The main fertilizers used were Diammonium Phosphate (DAP), Sulphate of Ammonia (SA), Calcium Ammonium Nitrate (CAN) and NPK (23:23:0), the amounts used were low even though the farmers were aware that soil fertility was low. No studies or attempts had been carried out to determine performance of rice genotypes and their heritability parameters for adaptation to low soil nitrogen (N) and phosphorous (P) conditions identified by the farmers as a major constraint in Kenya. Since the existing genetic base was narrow, accessions were acquired to broaden the variability of the local cultivars. However, their adaptation to local conditions was important for them to be useful. Evaluation of 390 accessions showed that genotypes and soil environments were highly significant for all the ten traits studied. The degree of genetic determination (H(2)) ranged from 8.0% for 1000 grain weight to 27% for top biomass. The phenotypic coefficient of variation of genotypes ranged from 12% for days to maturity to 149% for top biomass, while, that of days to maturity ranged from 14% to 160% for top biomass. The genetic advance (GA) had values ranging from, 0.2 for phosphorous tolerance to 1081 for grain yield, while the genetic advance expressed as percent of the mean was 6% for days to maturity and 88% for top biomass. The mean values for the ten characters studied had wide variability under the four soil environments with days to maturity ranging from 188 for genotype ARCCU1Fa1-L4P3-HB under both N and P application (N+P+) to 177 for genotype CT16333(1)-CA-1-M under none N and P application (N-P-) condition. The highest yielding genotype was CT16328-CA-18-M under none N and P application with 5916 kg ha(-1). The germplasm revealed usable variability under low soil N and P adaptation and thus warrant rice improvement for traits of interest to farmers. In order to establish the genetic factors controlling upland rice adaptation to contrasting soil N and P a study was conducted to determine genotypes with better performance under the prevailing farmers’ production environments. The GCA and SCA mean squares were significant and their interactions with environment were highly significant. The GCA:SCA ratios were mostly less than 1.0 for the majority of the traits under most of the soil N and P environments, indicating preponderance of nonadditive genetic effects. The maternal and nonmaternal mean squares were significantly different from zero (P<0.05) for most of the ten traits under study, indicating influence of cytoplasm effects and cytoplasm by nuclear gene interactions, respectively. The GCA effects for the parents were significant under different experimental environments but they had both positive and negative signs indicating different directions of influencing the trait of interest. The genotypes had both specific and broad adaptation as exhibited by their diverse rankings under different environments. The relationship between leaf and grain nitrogen (N) and phosphorous (P) with actual tolerance to low and high soil N and P conditions indicated significant genotypes, environments and genotypes by environment interactions. The analysis of genetic components gave highly significant GCA and SCA mean squares for the days to heading, anthesis and maturity, phosphorous and nitrogen tolerance, top biomass, plant height, number of panicles, 1000 seed weight and grain yield under the four soil experimental environments. The general combining ability (GCA) effects for parents were significant for both F(2) and F(3) segregants for the above ten traits and under the experimental conditions. The leaf and seed N and P contents gave significant genotype mean squares values for the three mega-environments. The GCA and SCA mean squares were significant for leaf and seed N and P for the three locations. The same case was observed for GCA effects for the parents, with all the nine parents showing significant values for leaf N and P for the three locations. The GCA:SCA mean square ratios were generally larger than 1.0 values for leaf N and grain P for the three locations. This clearly manifested the predominant role of additive gene action over the dominance effects and that improvement for tolerance to low soil N and P condition was practical. The fragrance in rice is an important quality traits and an experiment was conducted to establish the association of organoleptic test and the variations in SSR marker (RM223) among the parents. Plant leaves of the parental genotypes were sampled for DNA analysis using SSR markers RM223 and RM284. The RM223 was polymorphic while, RM284 gave monomorphic results. Four alleles were detected as follows: 146, 155, 161, and 163. The GCA:SCA ratio was 1.48 indicating that the fixable additive effects were greater than the nonadditive effects in inheritance of fragrance. Test for independence using Chi-square indicated that there was no association between organoleptic and variations in SSR markers. The performance of the aromatic lines was generally lower than that of non-aromatic lines for the agronomic traits. There was negative relationship between fragrant score and other agronomic traits such as grain yield across the three soil N and P regimes. The NERICA1 was found to be a good donor for aroma based on sensory testing and can be exploited in a breeding programme. Analysis on genetic components governing grain yield was investigated using Hayman’s analysis to generate various components of variation and to elucidate their potential, utilization, and provide a thorough understanding of their role in grain yield development and improvement. The additive gene action was significant for all cases of soil N and P environments and the two mega-environments. They were also far above the dominant gene effects, indicating the need to use mass selection in the early generations of segregating populations. The narrow sense heritability (h2(ns)) for upland rice grain yield was 21.52% and 4.22% under soil P and N conditions, respectively. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2010.