Rio Grande wild turkeys (Meleagris gallopavo intermedia) are a highly mobile, wide ranging, and secretive species located throughout the arid regions of Texas. As a result of declines in turkey abundance within the Edwards Plateau and other areas, Texas Parks and Wildlife Department initiated a study to evaluate methods for estimating Rio Grande wild turkey abundance. Unbiased methods for determining wild turkey abundance have long been desired, and although several different methods have been examined few have been successful. The study objectives were to: (1) review current and past methods for estimating turkey abundance, (2) evaluate the use of portable thermal imagers to estimate roosting wild turkeys in three ecoregions, and (3) determine the effectiveness of distance sampling from the air and ground to estimate wild turkey densities in the Edwards Plateau Ecoregion of Texas. Based on the literature review and the decision matrix, I determined two methods for field evaluation (i.e., infrared camera for detecting roosting turkeys and distance sample from the air and ground). I conducted eight ground and aerial forward-looking infrared (FLIR) surveys (4 Edwards Plateau, 3 Rolling Plains, and 1 Gulf Prairies and Marshes) of roost sites during the study. In the three regions evaluated, I was unable to aerially detect roosting turkeys using the portable infrared camera due to altitudinal restrictions required for safe helicopter flight and lack of thermal contrast. A total of 560 km of aerial transects and 10 (800 km) road based transects also were conducted in the Edwards Plateau but neither method yielded a sufficient sample size to generate an unbiased estimate of the turkey abundance. Aerial and ground distance sampling and aerial FLIR surveys were limited by terrain and dense vegetation and a lack of thermal contrast, respectively. Study results suggest aerial FLIR and ground applications to estimate Rio Grande wild turkeys are of limited value in Texas. In my opinion, a method for estimating Rio Grande wild turkey densities on a regional scale does not currently exist. Therefore, the Texas Parks and Wildlife Department should reconsider estimating trends or using indices to monitor turkey numbers on a regional scale.

Rio Grande wild turkeys (Meleagris gallopavo intermedia) are a highly mobile, wide ranging, and secretive species located throughout the arid regions of Texas. As a result of declines in turkey abundance within the Edwards Plateau and other areas, Texas Parks and Wildlife Department initiated a study to evaluate methods for estimating Rio Grande wild turkey abundance. Unbiased methods for determining wild turkey abundance have long been desired, and although several different methods have been examined few have been successful. The study objectives were to: (1) review current and past methods for estimating turkey abundance, (2) evaluate the use of portable thermal imagers to estimate roosting wild turkeys in three ecoregions, and (3) determine the effectiveness of distance sampling from the air and ground to estimate wild turkey densities in the Edwards Plateau Ecoregion of Texas. Based on the literature review and the decision matrix, I determined two methods for field evaluation (i.e., infrared camera for detecting roosting turkeys and distance sample from the air and ground). I conducted eight ground and aerial forward-looking infrared (FLIR) surveys (4 Edwards Plateau, 3 Rolling Plains, and 1 Gulf Prairies and Marshes) of roost sites during the study. In the three regions evaluated, I was unable to aerially detect roosting turkeys using the portable infrared camera due to altitudinal restrictions required for safe helicopter flight and lack of thermal contrast. A total of 560 km of aerial transects and 10 (800 km) road based transects also were conducted in the Edwards Plateau but neither method yielded a sufficient sample size to generate an unbiased estimate of the turkey abundance. Aerial and ground distance sampling and aerial FLIR surveys were limited by terrain and dense vegetation and a lack of thermal contrast, respectively. Study results suggest aerial FLIR and ground applications to estimate Rio Grande wild turkeys are of limited value in Texas. In my opinion, a method for estimating Rio Grande wild turkey densities on a regional scale does not currently exist. Therefore, the Texas Parks and Wildlife Department should reconsider estimating trends or using indices to monitor turkey numbers on a regional scale.

Rio Grande wild turkey (RGWT) abundance in portions of the Edwards Plateau has declined steadily since the late 1970s as compared to other areas of the Edwards Plateau where populations have exhibited no trend. The reasons for this decline remain unclear. Possible factors include changes in habitat, and increased human population. The overall objective of this study was to identify landscape changes and habitat characteristics that affect RGWT populations using spatial analysis and modeling at multiple spatial scales. Specific objectives for this study included the quantification of flood-induced landscape changes between 1972 and 1995 along the Medina River bottomlands and their impact on RGWT habitat, the quantification of landscape characteristics of stable and declining study sites in the Edwards Plateau, and the development and evaluation of a GIS-based habitat-suitability model for female RGWTs during the breeding season that will allow the assessment of the spatial distribution of adequate habitat in the Edwards Plateau.The analysis of the landscape characteristics along the North Prong Medina River due to flooding in 1978 had a negative impact on RGWT habitat. Changes in the spatial distribution of woody cover in the bottomlands and the removal of woody cover along riparian zones most likely limited habitat use and dispersal of RGWT along the North Prong Medina River. The analysis of landscape characteristics in sites with stable and declining of RGWTs populations showed that disturbance and a high proportion of woody cover were important factors influencing RGWT populations in areas where turkey numbers had declined. Landscape attributes were used as habitat variables to develop a habitat-suitability model for female RGWTs during the breeding season. The model performed well in characterizing high-suitability habitat for adult female RGWT during the breeding season in the study areas. The use of two scales relevant to RGWT provided important information about the high-suitability areas for female RGWT in stable and declining sites in the Edwards Plateau.

Sustainable management of wildlife populations relies on accurate estimates of population size as harvest recommendations are dependent on estimates of sustainable surplus. Techniques for surveying wild turkey populations in Texas are constrained by land access issues, requiring that new methods be developed for population monitoring. I evaluated a combined approach using patch-occupancy modeling at broad spatial scales and intensive double observer roost surveys at local scales to estimate Rio Grande wild turkey (Meleagris gallapavo intermedia) distribution and abundance. I flew replicated aerial surveys during 2007 and 2008 to evaluate distribution of Rio Grande wild turkeys in the south Texas Coastal Sand Plains. I used a double observer approach to estimate local scale abundance. I used a single observer approach to estimate temporal variation in roost use. Detection probabilities from aerial surveys ranged between 0.24 (SE = 0.031) and 0.30 (SE = 0.083). Spatial parameters that influenced distribution of wild turkeys included size of suitable roosting habitat patches and distance to the nearest suitable roosting habitat. I conducted 100 inter-patch double observer roost counts, with counts ranging between 0 to 183 individuals. Average detection probabilities for observers were ~0.90. Roost level occupancy was ~0.84 with detection probabilities between 0.69 (SE = 0.107) and 0.79 (SE = 0.091). Based on my results, aerial surveys combined with local abundance estimation may be one viable alternative to monitor turkey populations over large spatial scales, by reducing overall survey effort without loss of estimated precision.

Rio Grande wild turkey (RGWT) abundance in portions of the Edwards Plateau has declined steadily since the late 1970s as compared to other areas of the Edwards Plateau where populations have exhibited no trend. The reasons for this decline remain unclear. Possible factors include changes in habitat, and increased human population. The overall objective of this study was to identify landscape changes and habitat characteristics that affect RGWT populations using spatial analysis and modeling at multiple spatial scales. Specific objectives for this study included the quantification of flood-induced landscape changes between 1972 and 1995 along the Medina River bottomlands and their impact on RGWT habitat, the quantification of landscape characteristics of stable and declining study sites in the Edwards Plateau, and the development and evaluation of a GIS-based habitat-suitability model for female RGWTs during the breeding season that will allow the assessment of the spatial distribution of adequate habitat in the Edwards Plateau.The analysis of the landscape characteristics along the North Prong Medina River due to flooding in 1978 had a negative impact on RGWT habitat. Changes in the spatial distribution of woody cover in the bottomlands and the removal of woody cover along riparian zones most likely limited habitat use and dispersal of RGWT along the North Prong Medina River. The analysis of landscape characteristics in sites with stable and declining of RGWTs populations showed that disturbance and a high proportion of woody cover were important factors influencing RGWT populations in areas where turkey numbers had declined. Landscape attributes were used as habitat variables to develop a habitat-suitability model for female RGWTs during the breeding season. The model performed well in characterizing high-suitability habitat for adult female RGWT during the breeding season in the study areas. The use of two scales relevant to RGWT provided important information about the high-suitability areas for female RGWT in stable and declining sites in the Edwards Plateau.

Since 1970, Rio Grande wild turkey (Meleagris gallapavo intermedia) numbers in the southern region of the Edwards Plateau of Texas have been declining. Nest-site characteristics and invertebrate abundance were hypothesized as limiting wild turkey numbers in declining regions. Wild turkeys were trapped and fitted with mortality-sensitive radio transmitters on 4 study areas; 2 within a region of stable (northern Edwards Plateau) populations, and 2 within a region of declining populations. Monitoring occurred from February 2001 to August 2003. Nest-site locations were determined via homing during the breeding season. Following nesting attempts/completions, nest fate, vegetation height, visual obstruction, litter depth, percent cover, and cover scores of forbs, grass, litter, and bare ground at each nest site and surrounding area were sampled. This was done to determine if wild turkey hens selected nest sites with vegetative characteristics differing from surrounding habitat. Brood survival was calculated as >1 poult surviving to 2-weeks. Broods were followed for 6-weeks post-hatch or to brood failure. Invertebrates were collected, via sweep-net and D-vac, at each visually confirmed brood location and a paired random site to determine if wild turkey hens selected brood habitat based on invertebrate abundance. Analyses were performed to determine if invertebrate abundance differed between study regions. Turkey hens selected nest sites with greater visual obstruction and more litter depth on both regions of stable and declining turkey abundance. No vegetative differences were detected between stable and declining region nest sites. Frequency of Orthoptera was 3–5 times greater at nest sites on stable regions than declining regions in all 3 years. Orthoptera is a noted food source for young galliformes and comprised the majority of dry mass in invertebrate samples, nest sites and brood locations, on both the stable and declining regions. No differences in total invertebrate dry mass were detected between regional brood locations. Nest-site vegetative characteristics did not alter nest success between regions. The 2 overall objectives of this study were to determine if nest-site vegetation characteristics and invertebrate abundance affected wild turkey numbers in the Edwards Plateau. Regional differences in vegetative characteristics were not detected, thus not likely to be causing differences in turkey numbers between regions. Nest-site invertebrates were found to be 3–5 times greater at stable region nest sites, possibly giving wild turkey poults from stable regions greater initial chances of survival.

To determine possible causes of declining Rio Grande wild turkey (RGWT; Meleagris gallopavo intermedia) abundance in the southern Edwards Plateau, research was conducted on 4 sites, 2 with stable (S [SA and SB]; Kerr and Real counties) and 2 with declining (D [DA and DB]; Bandera County) RGWT populations. RGWTs were trapped, radio-tagged, and tracked. Ranges were constructed with 95% kernels. Data on brood survival and invertebrate and predator abundances were combined with range characteristics to assess habitat at a landscape scale. Annual range sizes did not differ in year 1, but were larger in S than in D in year 2. Range sizes in S increased from year 1 to year 2 while there was no change in range sizes in D. Range overlap was higher in D than S in both years. Movement distances remained consistent in S for both years, but were larger in D during year 1. During year 1 and year 2, RGWT females exhibited larger reproductive ranges and less range overlap in S. Invertebrate abundance for 4 insect orders was 2.5–15.9 times greater in S than in D while coyote abundance was 2–3 times greater in D than in S. Results were similar in year 3, with the exception of SB, where reproductive ranges and spatial arrangement were smaller than all other sites. My results refute the conventional assumption that larger ranges are indicative of poorer habitat quality. Range overlap suggests that useable space may have been limiting in D in the less productive year 2. In D, multiple broods used the same reproductive range, presumably depleting resources faster than in S. Greater predator abundance in D increased the risk of brood predation. The smaller reproductive spatial arrangement of SB females in year 3 correlates there being >3 times the percentage of females missing in other sites. If SB females moved further in year 3 than the detection distance of the radio telemetry equipment, the results would fit the pattern of greater dispersion distance in SA. RGWT females may attempt to separate themselves from other breeding females, possibly to avoid nest or brood predation and/or potential competition for brood resources.

Since 1970, Rio Grande wild turkey (Meleagris gallapavo intermedia) numbers in the southern region of the Edwards Plateau of Texas have been declining. Nest-site characteristics and invertebrate abundance were hypothesized as limiting wild turkey numbers in declining regions. Wild turkeys were trapped and fitted with mortality-sensitive radio transmitters on 4 study areas; 2 within a region of stable (northern Edwards Plateau) populations, and 2 within a region of declining populations. Monitoring occurred from February 2001 to August 2003. Nest-site locations were determined via homing during the breeding season. Following nesting attempts/completions, nest fate, vegetation height, visual obstruction, litter depth, percent cover, and cover scores of forbs, grass, litter, and bare ground at each nest site and surrounding area were sampled. This was done to determine if wild turkey hens selected nest sites with vegetative characteristics differing from surrounding habitat. Brood survival was calculated as >1 poult surviving to 2-weeks. Broods were followed for 6-weeks post-hatch or to brood failure. Invertebrates were collected, via sweep-net and D-vac, at each visually confirmed brood location and a paired random site to determine if wild turkey hens selected brood habitat based on invertebrate abundance. Analyses were performed to determine if invertebrate abundance differed between study regions. Turkey hens selected nest sites with greater visual obstruction and more litter depth on both regions of stable and declining turkey abundance. No vegetative differences were detected between stable and declining region nest sites. Frequency of Orthoptera was 3–5 times greater at nest sites on stable regions than declining regions in all 3 years. Orthoptera is a noted food source for young galliformes and comprised the majority of dry mass in invertebrate samples, nest sites and brood locations, on both the stable and declining regions. No differences in total invertebrate dry mass were detected between regional brood locations. Nest-site vegetative characteristics did not alter nest success between regions. The 2 overall objectives of this study were to determine if nest-site vegetation characteristics and invertebrate abundance affected wild turkey numbers in the Edwards Plateau. Regional differences in vegetative characteristics were not detected, thus not likely to be causing differences in turkey numbers between regions. Nest-site invertebrates were found to be 3–5 times greater at stable region nest sites, possibly giving wild turkey poults from stable regions greater initial chances of survival.

To determine possible causes of declining Rio Grande wild turkey (RGWT; Meleagris gallopavo intermedia) abundance in the southern Edwards Plateau, research was conducted on 4 sites, 2 with stable (S [SA and SB]; Kerr and Real counties) and 2 with declining (D [DA and DB]; Bandera County) RGWT populations. RGWTs were trapped, radio-tagged, and tracked. Ranges were constructed with 95% kernels. Data on brood survival and invertebrate and predator abundances were combined with range characteristics to assess habitat at a landscape scale. Annual range sizes did not differ in year 1, but were larger in S than in D in year 2. Range sizes in S increased from year 1 to year 2 while there was no change in range sizes in D. Range overlap was higher in D than S in both years. Movement distances remained consistent in S for both years, but were larger in D during year 1. During year 1 and year 2, RGWT females exhibited larger reproductive ranges and less range overlap in S. Invertebrate abundance for 4 insect orders was 2.5–15.9 times greater in S than in D while coyote abundance was 2–3 times greater in D than in S. Results were similar in year 3, with the exception of SB, where reproductive ranges and spatial arrangement were smaller than all other sites. My results refute the conventional assumption that larger ranges are indicative of poorer habitat quality. Range overlap suggests that useable space may have been limiting in D in the less productive year 2. In D, multiple broods used the same reproductive range, presumably depleting resources faster than in S. Greater predator abundance in D increased the risk of brood predation. The smaller reproductive spatial arrangement of SB females in year 3 correlates there being >3 times the percentage of females missing in other sites. If SB females moved further in year 3 than the detection distance of the radio telemetry equipment, the results would fit the pattern of greater dispersion distance in SA. RGWT females may attempt to separate themselves from other breeding females, possibly to avoid nest or brood predation and/or potential competition for brood resources.

The abundance of Rio Grande wild turkeys (Meleagris gallopavo intermedia) in the southeastern Edwards Plateau of Texas has declined since the late 1970s. Because knowledge of reproductive rates is important to understanding the dynamics of a population, radio-tagged hens were monitored during the 2005–2007 reproductive seasons to evaluate and compare reproductive parameters from areas with both declining and stable population trends. During January–March of 2005–2007, turkey hens were captured and radiotagged on 4 study areas; 2 within a region of stable turkey populations, and 2 within a region of declining populations. Monitoring occurred from January–July each season to determine nest- site locations. Nesting attempts, nest fate, clutch size, initiation date, and nest age were recorded. Nests were monitored ≥3 times weekly in order to estimate production parameters and daily nest survival. Poults were captured by hand and fitted with a 1.2 glue-on transmitter and monitored daily to estimate daily survival. Estimates show production was greater in stable regions than declining regions of the Edwards Plateau. Eighty-four percent of hens attempted to nest in the stable region and 67% attempted in the declining region. Eighteen of 102 nests were successful (≥1 egg hatched), in the stable region and 7 of 60 nests were successful in the declining region. Nest-survival analysis showed an influence of temporal variation within years, yet no differences in nest survival were detected between stable and declining regions. Poult survival also showed no difference between regions. The 2 overall objectives of this study were to determine if nesting parameters and nest survival were limiting factors in Rio Grande wild turkey abundance in the Edwards Plateau. Regional differences in production suggest the cause of the decline in the southeastern portion of the Edwards Plateau could be associated with lower reproductive output and consequently, success. Regional differences in nest survival were not detected, thus not likely to cause differences in turkey abundance between regions.