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Identification of coding and non-coding RNAs specific for early human retina development.January 2008 (has links)
Wong, Hoi Kin. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2008. / Includes bibliographical references (leaves 219-235). / Abstracts in English and Chinese. / Abstract --- p.i / Acknowledgements --- p.vi / Publications --- p.vii / Abbreviations --- p.viii / Chapter Chapter 1 --- Introduction / Chapter 1.1 --- Overview of Human Eye Developmental Process and Structure --- p.1 / Chapter 1.1.1 --- Developmental Process of the Eye --- p.2 / Chapter 1.1.2 --- Anatomy of Human Eye --- p.3 / Chapter 1.1.2.1 --- General Structure and Function of Human Eye --- p.4 / Chapter 1.1.2.2 --- Functional Architecture of Retina --- p.7 / Chapter 1.1.2.2.1 --- Photoreceptors --- p.8 / Chapter 1.1.2.2.2 --- Bipolar cell --- p.9 / Chapter 1.1.2.2.3 --- Horizontal cell --- p.10 / Chapter 1.1.2.2.4 --- Amacrine cell --- p.10 / Chapter 1.1.2.2.5 --- Interplexiform cell --- p.11 / Chapter 1.1.2.2.6 --- Muller glial cell --- p.11 / Chapter 1.1.2.2.7 --- Retinal ganglion cell --- p.12 / Chapter 1.1.3 --- Embryology of Human Eye --- p.12 / Chapter 1.1.3.1 --- Embryogenesis of Retina --- p.13 / Chapter 1.2 --- Identity of Retinal Stem Cells --- p.15 / Chapter 1.3 --- Molecular Mechanism of Early Retinogenesis --- p.17 / Chapter 1.3.1 --- Intrinsic Coding Genes for Retinogenesis --- p.18 / Chapter 1.3.1.1 --- PAX6 --- p.19 / Chapter 1.3.1.2 --- genes family --- p.20 / Chapter 1.3.1.3 --- RAX --- p.21 / Chapter 1.3.1.4 --- Repressor-type bHLH genes --- p.22 / Chapter 1.3.1.5 --- Activator-type bHLH genes --- p.23 / Chapter 1.3.1.6 --- POU4F gene family --- p.24 / Chapter 1.3.1.7 --- DLX1/DLX2 --- p.25 / Chapter 1.3.1.8 --- Others --- p.26 / Chapter 1.3.2 --- Extrinsic Factors for Retinogenesis --- p.27 / Chapter 1.3.2.1 --- Extrinsic morphogens --- p.27 / Chapter 1.3.2.2 --- Notch Signalling Pathway --- p.29 / Chapter 1.3.2.3 --- Sonic Hedgehog Signalling --- p.29 / Chapter 1.3.2.4 --- Wnt Signaling Pathway --- p.30 / Chapter 1.3.2.5 --- Cell Death Signalling during Retinogenesis --- p.31 / Chapter 1.4 --- Non-coding RNAs: MicroRNA --- p.34 / Chapter 1.4.1 --- Biogenesis and Post-Transcriptional Regulation Mechanism of MicroRNA --- p.35 / Chapter 1.4.2 --- Expression of Non-coding Genes in Retina and the Role in Retina Development --- p.37 / Chapter 1.5 --- Human Disorders Related to Retina Defect --- p.38 / Chapter Chapter 2 --- Project Objective and Outline --- p.42 / Chapter Chapter 3 --- Materials and Methods / Chapter 3.1 --- Samples Collection --- p.44 / Chapter 3.1.1 --- Isolation of Retina --- p.45 / Chapter 3.1.2 --- Samples Processing and Storage --- p.46 / Chapter 3.1.3 --- Total RNA extraction --- p.46 / Chapter 3.2 --- RNA Quality Control --- p.47 / Chapter 3.2.1 --- NanoDrop ND-1000 --- p.47 / Chapter 3.2.2 --- Agilent 2100 Bioanalyzer --- p.48 / Chapter 3.3 --- Expression profiling by Microarray Experiments --- p.51 / Chapter 3.3.1 --- Gene Expression Microarray --- p.52 / Chapter 3.3.1.1 --- Prepare Labelling Reaction --- p.52 / Chapter 3.3.1.2 --- Purify the Labelled/Amplified RNA --- p.54 / Chapter 3.3.1.3 --- Prepare Hybridization Samples --- p.55 / Chapter 3.3.1.4 --- "Hybridization, Washing and Scanning" --- p.55 / Chapter 3.3.1.5 --- Gene Expression Data Quality and Acquisition --- p.57 / Chapter 3.3.2 --- MicroRNA Microarray --- p.58 / Chapter 3.3.2.1 --- Dephosphorylation and Labeling Reaction --- p.58 / Chapter 3.3.2.2 --- Gel Filtration to Purify the Labelled RNA --- p.60 / Chapter 3.3.2.3 --- Hybridization Sample Preparation --- p.61 / Chapter 3.3.2.4 --- Prepare the Hybridization Assembly --- p.61 / Chapter 3.3.2.5 --- Washing and scanning --- p.62 / Chapter 3.3.2.6 --- MiRNA Data Quality and Acquisition --- p.62 / Chapter 3.4 --- Statistical Analysis --- p.63 / Chapter 3.4.1 --- Data Normalization --- p.63 / Chapter 3.4.2 --- Significant tests --- p.64 / Chapter 3.4.3 --- Clustering --- p.65 / Chapter 3.5 --- Quantitative Real-Time Polymerase Chain Reaction --- p.65 / Chapter 3.5.1 --- Reverse Transcription --- p.65 / Chapter 3.5.2 --- Quantitative Real-Time PCR --- p.66 / Chapter 3.6 --- Histochemical Staining --- p.67 / Chapter 3.6.1 --- Haematoxylin-and-eosin staining --- p.67 / Chapter 3.6.2 --- Immunohistochemical staining --- p.67 / Chapter 3.6.3 --- In Situ Hybridization (ISH) for MicroRNA --- p.69 / Chapter Chapter 4 --- Results / Chapter 4.1 --- Temporal Gene Expression of Human Fetal Retina --- p.71 / Chapter 4.1.1 --- Tissue specificity by Principal Component Analysis --- p.73 / Chapter 4.1.2 --- mRNA Expression Profiling from Gestational Week 9 to Week 15 --- p.74 / Chapter 4.1.3 --- Characterization of Known Eye Specific Genes in Early Gestation --- p.76 / Chapter 4.2 --- Differential Expressed Genes between Gestational Week 9 and Week 15 --- p.78 / Chapter 4.2.1 --- Unsupervised Clustering between Gestational Week 9 and Week 15 --- p.78 / Chapter 4.2.2 --- Statistical Calculation of Significantly Differential Expressed Gene by Using GeneSpring 7.3.1 --- p.79 / Chapter 4.2.3 --- Statistical Clustering of Unique Expression Profile by Using Self-organizing Map (SOM) Clustering --- p.81 / Chapter 4.2.4 --- Functional Annotation of Differentially Expressed Genes --- p.82 / Chapter 4.3 --- Quantitation of Candidate Eye-Specific Genes by Real-Time PCR --- p.84 / Chapter 4.4 --- Novelty of the human Fetal Retina mRNAs --- p.85 / Chapter 4.5 --- MicroRNA Expression Profiling in Early Retinogenesis --- p.87 / Chapter 4.6 --- Correlation between Coding and Non-coding RNA According to the Expression Pattern on Microarray --- p.90 / Chapter 4.6.1 --- MiRNA Target Gene Analysis --- p.90 / Chapter 4.6.2 --- Correlation analysis between miRNA and its target transcripts according to Global gene expression profile in retina --- p.91 / Chapter Chapter 5 --- Discussion / Chapter 5.1 --- Characterization of the Gene Expression Profile --- p.94 / Chapter 5.1.1 --- Indigenous Genes Expression in Human Fetal Retina --- p.95 / Chapter 5.1.1.1 --- Overview of the mRNA Expression --- p.95 / Chapter 5.1.1.2 --- Characterization by Gene Ontology and Human KEGG Pathway --- p.99 / Chapter 5.1.2 --- Gene Expression Data Comparison from Public Database --- p.101 / Chapter 5.2 --- Stage Specific Genes Expressed on Week 9 and Week 15 --- p.102 / Chapter 5.2.1 --- Characterization of Stage Specific Genes by Gene Ontology Analysis --- p.103 / Chapter 5.2.2 --- Known and new retinal-specific Genes Upregulated on Gestational Week 9 --- p.104 / Chapter 5.2.2.1 --- ALDH1A1A/STRA6 --- p.104 / Chapter 5.2.2.2 --- GAL --- p.105 / Chapter 5.2.2.3 --- CNTN2/SLIT102 --- p.107 / Chapter 5.2.2.4 --- LMINC3 --- p.108 / Chapter 5.2.2.5 --- DLX1/DLX2 --- p.109 / Chapter 5.2.3 --- Genes Upregulated on Gestational Week 15 --- p.110 / Chapter 5.2.3.1 --- RCVN/RCV1 --- p.110 / Chapter 5.2.3.2 --- PAVLB --- p.111 / Chapter 5.2.3.3 --- Presence of Synaptic and Neuronal Signal Transduction Activities in Week 15 Retina --- p.111 / Chapter 5.2.3.4 --- Cell differentiation and Development --- p.112 / Chapter 5.3 --- Expression Characteristic of MicroRNA in Embryonic Retina and the Related Targets --- p.114 / Chapter 5.3.1 --- Other Highly Expressed MiRNAs --- p.115 / Chapter 5.3.2 --- Highly Expressed miRNAs and their Potential Predicted target Function --- p.117 / Chapter 5.3.2.1 --- MiR-124a --- p.117 / Chapter 5.3.3 --- Expression Trends and the Corresponding MiRNAs Analysis --- p.119 / Chapter 5.4 --- Potential Studies for Further Investigation --- p.120 / Chapter Chapter 6 --- Conclusions --- p.122 / Tables and Figures / Figure 1.1 Embryogenesis of early human eye development --- p.124 / Figure 1.2 The anatomy of human eye- --- p.125 / Figure 1.3 Schematic diagram of general retina structure --- p.126 / Figure 1.4 Schematic diagram of photoreceptor cells (cone and rod) --- p.127 / Figure 1.5 The proliferation and differentiation of neural retina between gestational week 6 and week 8 --- p.128 / Figure 1.6 The time line for retinogenesis between the 2nd month and the 6th month of human gestation and the corresponding time line of mouse retinogenesis between the E12 and the Pll --- p.129 / Figure 1.7 Schematic diagram of Notch signaling pathway --- p.130 / Figure 1.8 Summary of the homeobox gene and bHLH gene expression to trigger particular cell type differentiation from retinal progenitor cells --- p.131 / Figure 1.9 Summary of molecular interactions during retinogenesis --- p.132 / Figure 1.10 Signal transduction pathway of sonic hedgehog --- p.133 / Figure 1.11 Biogenesis of microRNA (miRNA) and posttranscriptional regulation of mRNA --- p.134 / Figure 1.12 Summary of the expression of various miRNAs in retina and other neuronal tissues reported in animal studies --- p.135 / Figure 1.13 Number of the mapped loci and the identified retina disease genes between 1980 and 2008 --- p.136 / Figure 3.1 A typical RNA optical density (OD) profile exported from Nanodrop --- p.137 / Figure 3.2 Detection of Cy3 labelled cRNA and the corresponding specificity by Nanodrop ND-1000 --- p.138 / Figure 3.3 RNA quality profile generated from Agilent2100 Bioanalyzer --- p.139 / Figure 3.4 Amplified and fluorescence labelled cRNA profile from Agilent 2100 Bioanalyzer --- p.140 / Figure 3.5 Scatter plot of the fluorescent same fibroblast sample labelled Cy3 and Cy5 separately --- p.141 / Figure 3.6 Scatter plot of 2 identical placenta miRNA separately hybridized on 2 arrays on the same chip --- p.142 / Equation 3.1 Calculation of specificity from fluorescence labelled cRNA samples --- p.143 / Table 3.1 Information of human fetal retina samples involved in this study --- p.144 / Table 3.2 Summary of sample selection criteria by using NanoDrop ND-1000 and Agilent 2100 Bioanalyzer --- p.145 / Figure 4.1 Histogram of expressed probes which express on the gene expression microarrays from gestational week 9 to week 15 --- p.146 / Figure 4.2 Gene ontology (GO) study in nervous system development --- p.147 / Figure 4.3 Gene ontology (GO) study in sensory perception --- p.148 / Figure 4.4 Gene ontology (GO) study in cell growth --- p.149 / Figure 4.5 Gene ontology (GO) study in biological process --- p.150 / Figure 4.6 Box plot of 27 samples from gene expression microarray experiment --- p.151 / "Figure 4.7 Principal component analysis of fetal retina tissues, retinoblastoma tissues and retinoblastoma cell line" --- p.152 / Figure 4.8 Hierarchical clustering of human fetal retina tissues and retinoblastoma samples in gene expression microarray experiments --- p.153 / Figure 4.9 Gene expression profile of human fetal retina from gestational week 9 to week 15 --- p.154 / Figure 4.10 Gene expression profiles of PAX6 and SOX3 --- p.155 / Table 4.3 POU4F1 expression data generated from gene expression microarray from different gestational weeks --- p.156 / Figure 4.11 Graphical illustration of the expression pattern of POU4F1 in retina from gene expression microarray between human gestational week 9 to week 15 --- p.156 / Figure 4.12 Gene expression results of POU4Fl from real-time --- p.157 / Figure 4.13 Retinal ganglion cell differentiation in human fetal retina --- p.158 / Figure 4.14 Hierarchical clustering of human fetal retina tissues comparing gestational week 9 and week 15 with retinoblastoma samples --- p.159 / Figure 4.15 H&E staining of human fetal retina tissues from gestational week 9 to week 15 --- p.160 / Figure 4.16 Scatter plot of differentially expressed genes fold change equal to 2 between gestational week 9 and week 15 --- p.161 / Figure 4.17 Volcano plot of differentially statistical significant expressed genes on gestational week 9 and week 15 --- p.162 / Figure 4.18 A continuous line plot for the differential expressed genes at gestational week 9 and week 15 --- p.163 / Figure 4.19 Self-organizing map clustering (SOM) of the expression library base on the result Figure 4.9 --- p.164 / Figure 4.20 Selected SOM clusters from the Figure 4.22 --- p.165 / Figure 4.21 Gene ontology study of genes commonly expressed in differential expressed gene list (Figure 4.20) and the selected gene clusters in SOM clustering- --- p.166 / "Figure 4.22 Gene ontology diagram for grouping 385 genes differentially expressed on gestational week 15 and commonly found in SOM 226}0بcluster 6,1' with different functions and characteristics" --- p.167 / Figure 4.23 Results comparison between microarray experiment and quantitative real-time PCR validation of temporal expressed genes on gestational week 9 --- p.168 / Figure 4.24 Results comparison between microarray experiment and quantitative real-time PCR validation of temporal expressed genes on gestational week 15 --- p.169 / Figure 4.25 Gene cluster specifically find in this study by compare with RetinaCentral database --- p.170 / Figure 4.26 Histogram of miRNA expression distribution of all 470 miRNAs --- p.171 / Figure 4.27 Hierarchical clustering of miRNA expression array on human fetal retinas and retinoblastoma samples --- p.172 / Figure 4.28 The correlation distribution of miR-124a and mRNAs --- p.173 / Figure 4.29 Expression profiles of GAL and the corresponding- miRNAs searched from TargetScan 4.1 --- p.174 / Figure 4.30 Scatter plot of miR-345 and GAL --- p.175 / Figure 4.31 Expression profiles of DLX1 and the corresponding miRNAs searched from TargetScan 4.2 --- p.176 / Figure 4.32 Expression profiles of DLX2 and the corresponding miRNAs searched from TargetScan 4.1 --- p.177 / Figure 4.33 In situ hybridization (ISH) of miR-182 of human fetal retina on gestational week 9 and week 17 --- p.178 / Figure 4.34 Expression of miR-182 in retinoblastoma tissue --- p.179 / Table 4.1 KEGG pathway analysis from GeneSpring 7.3.1 --- p.180 / Table 4.2 Expression signals of the genes associated with retina development --- p.181 / Table 4.6 Assorted gene ontology terms under the category of molecular function and biological process --- p.182 / Figure 4.7 Selected genes from common gene list on week 9 and week 15 for quantitative real-time PCR --- p.183 / "Table 4.8 Selected genes with expression intensity higher than100,000 with the corresponding UniGene ID, Genbank ID and function(s)" --- p.184 / Table 4.9 The top 25 highly expressed miRNA and the corresponding signal and recent finding on the neuronal organ --- p.188 / Table 4.10 Pathway analysis from GeneSpring shows the related pathway and target genes from the top 10 highly expressed miRNAs --- p.190 / Table 4.11 Genes and miRNA with expression correlation coefficient smaller than -0.7 and gene expression signal higher than / Figure 5.1 Summary of pathway interactions from KEGG pathway analysis --- p.193 / Figure 5.2 Expression tread of ALDHlAl normalized by control signals --- p.194 / Figure 5.3 The retinoic acid metabolize pathway between egg yolk and placenta --- p.195 / Figure 5.4 The expression of other genes involved in retinoic acid metabolic pathway --- p.196 / Figure 5.5 Expression signal of various galanin receptors in our data --- p.197 / Figure 5.6 Expression signal of CNTN2 from gestational week 9 to week 15 --- p.198 / Figure 5.7 Expression of DLX1 and DLX2 and the corresponding correlation --- p.199 / Figure 5.8 Expression trend of RCVN from gestational week 9 to week 15 --- p.200 / "Figure 5.9 Expression correlation between ALDH1A1 and miR-124a, -9 and -9*" --- p.201 / Figure 5.10 Expression signals of REST and SCP1 in fibroblast,fetal retina and retinoblastoma tissues --- p.202 / Table 5.1 Congenital diseases from RetNet and the corresponding gene expression signal on our data --- p.203 / Table 5.2 Expression correlation coefficient of miR-124a and mRNAs from our expression data --- p.206 / Appendices / Appendix I - Category of Retinal Disease and their Related Gene and Locus (RetNet:http://www.sph.uth.tmc.edu/RetNet/) Last updated June 13,2008 --- p.207 / Appendix II - List of Taqman probes designed for real-time PCR with gene name and the corresponding annotations and sequence --- p.210 / "Appendix III - Functional annotated of highly expressed genes (signal > 100,000) from the KEGG pathways" --- p.211 / Appendix IV - LOWESS normalized expression signal with corresponding annotation and chromosome location --- p.217 / Appendix V - Various gene lists or probes lists used in the data analysis --- p.218 / References --- p.719
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Identification and application of functional RNAsHesselberth, Jay Richard 28 August 2008 (has links)
Not available / text
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Frameshifting as a tool in analysis of transfer RNA modification and translation /Leipuvienė, Ramunė, January 2004 (has links)
Diss. (sammanfattning) Umeå : Univ., 2004. / Härtill 4 uppsatser.
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New techniques for analysing RNA structure /Freyhult, Eva, January 2004 (has links) (PDF)
Licentiatavhandling Uppsala, Univ : 2004. / Härtill 4 uppsatser.
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Viral protein and RNA synthesis in barley protoplasts inoculated with native, fractionated, and chemically-modified Brome Mosaic Virus RNAKiberstis, Paula Ann. January 1982 (has links)
Thesis (Ph. D.)--University of Wisconsin--Madison, 1982. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Bibliography: leaves 168-176.
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Analysis of Human Transfer RNA Gene HeteroclustersChang, Yung-Nien 12 1900 (has links)
Two phage lambda clones encompassing human tRNA genes have been isolated from a human gene library harbored in bacteriophage lambda Charon-UA. One of the clones (designated as hLeuU) containing a 20-kb human DNA fragment was isolated and found to contain a cluster of four tRNA genes. An 8.2-kb Hindlll fragment encompassing the four tRNA genes was isolated from the 20-kb fragment and subcloned into pBR322 for restriction mapping and DNA sequence analysis. The four tRNA genes are arranged as two tandem pairs with the first pair containing a proline tRNAAGQ gene and a leucine tRNAAAQ gene and the second pair containing another proline tRNAAGG gene and a threonine tRNAuQU gene. The two pairs are separated about 3 kb from each other, and the leucine tRNAAAG gene is of opposite polarity from the other three tRNA genes. The tRNA transcription units were sequenced by a unidirectional deletion dideoxyribonucleotide chain-termination method in the M13mpl8 and 19 vectors. The coding regions of the four tRNA genes contain characteristic internal split promoter sequences and do not encode intervening sequences nor the CCA trinucleotide found in mature tRNAs. The proline t R N A A G G gene is separated from the leucine t R N A A A Q gene by a 725-bp intergenic region and the second proline t R N A A G Q is 315 bp downstream of the threonine t R N A U G U gene. The coding sequences of the two proline tRNA genes are identical. The 3'-flanking regions near the 3*-ends of these four tRNA genes have typical RNA polymerase III termination sites of at least four c o n s e c u t i v e T nt. There is no homology between the 5'-flanking regions of these genes. All four tRNA genes are potentially functional, since they are transcribed by RNA polymerase III in an in vitro HeLa cell extract. Another phage lambda clone (designated as XhLeu8) was also found to contain four tRNA genes. One of the tRNA genes was characterized by DNA sequencing. The tRNA gene has an anticodon for leucine, but has three base substitutions from the leucine tRNA^A G and the leucine tRNAAAQ gene of AhLPT, occurring in the D-stem, D-loop and T-loop regions. The substitution in the T-loop is a C to T transition at an otherwise invariant position of the consensus sequence, 5'-GTTC-3'» within the B-block of the internal split promotor. Thus, this gene may more properly be classified as a leucine tRNA pseudogene.
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The novel ugagau hexaloop RNA structure, dipolar coupling refinement, and transactivation /Leeper, Thomas January 2001 (has links)
Thesis (Ph. D.)--University of Missouri-Columbia, 2001. / Typescript. Vita. Includes bibliographical references. Also available on the Internet.
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The novel ugagau hexaloop RNA structure, dipolar coupling refinement, and transactivationLeeper, Thomas January 2001 (has links)
Thesis (Ph. D.)--University of Missouri-Columbia, 2001. / Typescript. Vita. Includes bibliographical references. Also available on the Internet.
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Selective ADAR editing and the coordination with splicing /Källman, Annika, January 2004 (has links)
Diss. (sammanfattning) Stockholm : Univ., 2004. / Härtill 4 uppsatser.
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RELATIVE ACCEPTOR ACTIVITIES OF FOUR TRANSFER RNA FAMILIES EXTRACTED FROM SUSPENSION CULTURES OF DAUCUS CAROTA L.Helm, Kenneth Winchell. January 1984 (has links)
No description available.
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