Thesis (Ph. D.)--Swedish University of Agricultural Sciences, 2001. / Includes bibliographical references.

No description available.

Thesis (Ph. D.)--Columbia University. / Includes bibliographical references.

No description available.

The aim of this study was to determine propagation methodology of L. sericea taking into consideration the effect of season and storage. The response of tip and basal cuttings of L. sericea to three hormonal treatments and three growth media was investigated. Seed germination response to five constant temperatures and four pre-sowing treatments was also investigated. Using NMR spectroscopy, the effects of season and storage on metabolites in leaves of L. sericea were investigated. All treatments did not initiate or improve rooting of L sericea cuttings. Seed germination percentage was significantly affected by temperature and pre-sowing treatment compared to control. The optimum seed germination percentage was obtained at 15°C (53%) when treated with smoke water. Seasonal variation affected leaf metabolite profiles. Storage did not affect the antibacterial activity of L. sericea leaves and those harvested in winter showed an MIC value of ≤3.90μg/mL when tested on Propionibacterium acnes / Agriculture, Animal Health and Human Ecology / M. Sc. (Agriculture)

Thesis (Ph. D.)--Cornell University, 1922. / Cover title. Includes bibliographical references.

Thesis (doctoral)--Swedish University of Agricultural Sciences, 2000. / Includes bibliographical references.

Diss. (sammanfattning) Alnarp : Sveriges lantbruksuniversitet, 2006. / Härtill 5 uppsatser.

Orientador: Dulce Helena Siqueira Silva / Resumo: O presente trabalho descreve o estudo químico das folhas e galhos de Prunus myrtifolia (L.) Urban (Rosaceae), exemplar coletado na Fazenda Campininha em São Paulo, SP. A química do gênero Prunus é determinada pela ocorrência de glicosídeos cianogênicos , terpenóides, flavonóides e outras substâncias fenólicas. Os extratos obtidos a partir de folhas e galhos de P. myrtifolia foram fracionados através de técnicas cromatográficas diversas que forneceram 31 substâncias, sendo 3 consideradas inéditas. Suas estruturas foram determinadas através de dados espectrométricos (RMN, EM, UV, entre outros), bem como através da utilização de técnicas hifenadas tais como CLAE-UV e CLAE-EM. O fracionamento cromatográfico guiado por testes químicos demonstrou o potencial de atividade antioxidante de flavonóides e arilpropanóides isolados de folhas e galhos de P. myrtifolia. A mistura de esteróides estigmasterol e sitosterol apresentou atividade antifúngica moderada frente a fungos fitopatógenos do gênero Cladosporium enquanto que os glicosídeos cianogênicos prunasina e durrina, além do ácido prunasínico apresentaram atividade antifúngica contra Cândida krusei e C. Albicans. A atividade citotóxica para Sacharomyces cerevisea apresentada pelos extratos brutos não foi mais observada após os procedimentos de fracionamento cromatográfico. Entretanto, a mistura de triterpenos pentacíclicos triidroxilados obtida a partir do extrato etanólico de galhos, apresentou atividade antitumoral para células de câncer de cólon, de mama e do sistema nervoso central humano. Os resultados obtidos com esse estudo químico corroboram dados da literatura sobre Prunus e Rosaceae e poderão contribuir com estudos de quimiossistemática. / Abstract: This work reports the results from chemical study of leaves and branches of Prunus myrtifolia (L.) Urban. (Rosaceae), collected at Fazenda Campininha, São Paulo. The chemistry of genus Prunus is determined by occurrence of cyanogenic glicosides, terpenoids, flavonoids and other phenolic compounds. Ethanolic extracts obtained from leaves and branches of Prunus myrtifolia were fractionated through chromatographic methods that led to the isolation of 28 compounds wich were previously descibed and 3 additional new compounds. Their structures were determined by spectrscopic data (NMR, MS, UV, etc), and also by the use of hyphenated technics (HPLC_UV and HPLC-MS). The fractionation guided by chemical and biological assays demonstrated the atioxidant activity of 6 flavonoids, arilpropanoid derivatives and other isolates from leaves and branches of P. myrtifolia. The mixture of steroids stigmasterol and sitosterol obtained showed moderate activity against phytopatogenic fungus of genus Cladosporium and the cyanogenic glycosides prunasin and dhurrin, and prunasinic acid showed antifungal activity against human Candida pathogens. The cytotoxic activity against Sacharomyces cereviseae presented for crude ethanol extracts were not observed anymore after the chromatograpic frationation procedures, although the trihydroxylated triterpenoids obtained from branches ethanol extract inhibited growth of colon, breast and CNS human cancer cells. Additionally, the results obtained with this work corroborate chemical data about genus Prunus and Rosaceae and therefore contribute with chemosystematic studies. / Doutor

Made available in DSpace on 2014-06-11T19:35:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2005Bitstream added on 2014-06-13T19:05:25Z : No. of bitstreams: 1 santos_la_dr_araiq.pdf: 1408996 bytes, checksum: 5871577900c69d41b11bdee03b41554a (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / O presente trabalho descreve o estudo químico das folhas e galhos de Prunus myrtifolia (L.) Urban (Rosaceae), exemplar coletado na Fazenda Campininha em São Paulo, SP. A química do gênero Prunus é determinada pela ocorrência de glicosídeos cianogênicos , terpenóides, flavonóides e outras substâncias fenólicas. Os extratos obtidos a partir de folhas e galhos de P. myrtifolia foram fracionados através de técnicas cromatográficas diversas que forneceram 31 substâncias, sendo 3 consideradas inéditas. Suas estruturas foram determinadas através de dados espectrométricos (RMN, EM, UV, entre outros), bem como através da utilização de técnicas hifenadas tais como CLAE-UV e CLAE-EM. O fracionamento cromatográfico guiado por testes químicos demonstrou o potencial de atividade antioxidante de flavonóides e arilpropanóides isolados de folhas e galhos de P. myrtifolia. A mistura de esteróides estigmasterol e sitosterol apresentou atividade antifúngica moderada frente a fungos fitopatógenos do gênero Cladosporium enquanto que os glicosídeos cianogênicos prunasina e durrina, além do ácido prunasínico apresentaram atividade antifúngica contra Cândida krusei e C. Albicans. A atividade citotóxica para Sacharomyces cerevisea apresentada pelos extratos brutos não foi mais observada após os procedimentos de fracionamento cromatográfico. Entretanto, a mistura de triterpenos pentacíclicos triidroxilados obtida a partir do extrato etanólico de galhos, apresentou atividade antitumoral para células de câncer de cólon, de mama e do sistema nervoso central humano. Os resultados obtidos com esse estudo químico corroboram dados da literatura sobre Prunus e Rosaceae e poderão contribuir com estudos de quimiossistemática. / This work reports the results from chemical study of leaves and branches of Prunus myrtifolia (L.) Urban. (Rosaceae), collected at Fazenda Campininha, São Paulo. The chemistry of genus Prunus is determined by occurrence of cyanogenic glicosides, terpenoids, flavonoids and other phenolic compounds. Ethanolic extracts obtained from leaves and branches of Prunus myrtifolia were fractionated through chromatographic methods that led to the isolation of 28 compounds wich were previously descibed and 3 additional new compounds. Their structures were determined by spectrscopic data (NMR, MS, UV, etc), and also by the use of hyphenated technics (HPLC_UV and HPLC-MS). The fractionation guided by chemical and biological assays demonstrated the atioxidant activity of 6 flavonoids, arilpropanoid derivatives and other isolates from leaves and branches of P. myrtifolia. The mixture of steroids stigmasterol and sitosterol obtained showed moderate activity against phytopatogenic fungus of genus Cladosporium and the cyanogenic glycosides prunasin and dhurrin, and prunasinic acid showed antifungal activity against human Candida pathogens. The cytotoxic activity against Sacharomyces cereviseae presented for crude ethanol extracts were not observed anymore after the chromatograpic frationation procedures, although the trihydroxylated triterpenoids obtained from branches ethanol extract inhibited growth of colon, breast and CNS human cancer cells. Additionally, the results obtained with this work corroborate chemical data about genus Prunus and Rosaceae and therefore contribute with chemosystematic studies.