Made available in DSpace on 2014-12-17T15:18:08Z (GMT). No. of bitstreams: 1 FladjuleRSS.pdf: 330062 bytes, checksum: 22e7d9836e1ea44b75c1f00b2ab22a60 (MD5) Previous issue date: 2008-03-28 / Bacterial meningitis (BM) is still an important infectious disease causing death and disability. Invasive bacterial infections of the central nervous systems (CNS) generate some of the most powerful inflammatory responses known, which contributes to neuronal damage. The DNA microarray technology showed alterations in the kynurenine (KYN) pathway that is induced in BM and other diseases associated with inflammation, leading to brain injury. Our main aim was to search SNPs previously described in the KYN path enzymes to investigate a putative association of this SNPs with imbalanced in this pathway in patients with BM. The patients included in this study were 33 males and 24 females, with ages varying from 02 months to 68 years. SNPs were located inside of the domain conserved in KYNU, IDO, KATI and KATII. Primers were designed for analysis of SNPs already described by PIRA-PCR followed by RFLP. The analysis of KYNU+715G/A SNP found a heterozygous frequency of 0.033. We did not found the variant allele of SNP KYNU+693G/A, KATI+164T/C, KATII+650C/T and IDO+434T/G. Despite of previews studies showing the importance of KYN pathway we did not found one association of these SNPs analyzed with susceptibility or severity of MB in study population. / A meningite bacteriana (MB) ? uma doen?a infecciosa que causa morte e deixa graves seq?elas. Infec??es bacterianas do sistema nervoso central (SNC) geram uma das mais poderosas respostas inflamat?rias conhecidas, a qual contribui para os danos neuronais. Atrav?s de an?lises por Microarray foi poss?vel observar altera??es na Rota da Quinurenina (RQ) que s?o induzidas na MB e em outras doen?as associadas com inflama??o, levando a inj?ria cerebral. A RQ tem um papel crucial na patog?nese da MB, produzindo neurotoxinas e esp?cies reativas de oxig?nio. Nosso principal objetivo foi buscar SNPs previamente descritos no banco de dados do NCBI em enzimas da RQ e investigar uma poss?vel associa??o destes SNPs com as altera??es da RQ em pacientes com MB. Os pacientes inclu?dos neste estudo foram 33 homens e 24 mulheres, com idades variando entre 02 meses a 68 anos. Os SNPs foram localizados dentro do dom?nio conservado da cadeia polipept?dica das enzimas KYNU, IDO, KATI e KATII. Primers foram desenhados para an?lises do SNPs atrav?s da t?cnica do PIRA-PCR seguido por RFLP. A an?lise do SNP KYNU+693G/A mostrou uma freq??ncia de heterozigosidade de 0,033. N?s n?o encontramos freq??ncia al?lica na popula??o estudada para os SNPs KYNU+693G/A, KATI+164T/C, KATII650C/T e IDO+434T/G. Apesar de estudos anteriores mostrarem a import?ncia da RQ, n?o foi encontrada uma associa??o dos SNPs estudados com a susceptibilidade ou a severidade das seq?elas da MB na popula??o em estudo.