Lic.-avh. (sammanfattning) Alnarp : Sveriges lantbruksuniv., 2003. / Härtill 3 uppsatser.

Thesis (Ph. D.)--University of Adelaide, Dept. of Plant Science, 1996? / Includes bibliographical references (leaves 307-329).

Thesis (Ph. D.)--Michigan State University. Plant Breeding and Genetics Program, 2006. / Title from PDF t.p. (viewed on Nov. 12, 2008) Includes bibliographical references. Also issued in print.

Thesis (M.S.)--Oregon State University, 1993. / Includes mounted photographs. Typescript (photocopy). Includes bibliographical references (leaves 64-86). Also available on the World Wide Web.

Thesis (M.S.)--Oregon State University, 1992. / Typescript (photocopy). Includes bibliographical references (leaves 69-77). Also available online.

Thesis (Ph. D.)--Washington State University, December 2006. / Includes bibliographical references.

Please read the Resume in the section 06resume of this document / Dissertation (MSc (Agric))--University of Pretoria, 2006. / Microbiology and Plant Pathology / unrestricted

Three independent studies were conducted for spot blotch (Bipolaris sorokiniana), Fusarium head blight (FHB) (Fusarium graminearum), and root rot diseases (Fusarium species and B. sorokiniana). Histopathology of compatible and incompatible interactions between different pathotypes of B. sorokiniana and different genotypes of barley was examined with red fluorescent protein-tagged fungal isolates. The fungus penetrated the host cell wall and developed multicellular globular infection hyphae (IH) in the lumen of epidermal cells, but infected epidermal cells appeared to be alive till 16 hours post-inoculation (HPI). In the susceptible plants, the tip of IH was found to grow ahead of the dead tissue and invade the surrounding live mesophyll cells, whereas growth of IH in the resistant plants was restricted to the dead tissue after 20 HPI. The amount of H2O2 accumulation and the fungal biomass were also significantly higher in the susceptible hosts than in the resistant hosts. To map resistance to FHB, two populations consisting 130 doubled haploid lines from the cross Grandin × PI277012 and 237 recombinant inbred lines from the cross Bobwhite × ND2710 were phenotyped and genotyped. QTL for Type I resistance were identified on chromosomes 1A, 2B, 4B, 5B and 6B in the GP population. These QTL explained 10.7-19 % of the total phenotypic variation. With the BN population, QTL for Type I resistance were identified on chromosomes 2A, 5A and 6B, explaining 6.2-13.7% of the total phenotypic variation. To assess the prevalence, incidence and severity of wheat crown rot (CR) and common root rot (CRR) in ND, wheat root samples were collected from fields across the state in 2012, 2013, and 2014. Fungal isolations indicated that B. sorokiniana was most frequently recovered in all sampled years. Seedling tests on ten spring wheat lines showed that Glenn was the least susceptible while Steele-ND was the most susceptible to one F. culmorum isolate and one B. sorokiniana isolate tested. Evaluation of 20 spring wheat genotypes for reaction to CRR at the adult plant stage showed that Freyr and RB07 were more resistant while Len and Briggs were more susceptible to CRR compared to other wheat genotypes evaluated. / North Dakota Wheat Commission, / Minnesota Wheat Research and Promotion Council / ND State Board of Agricultural Research and Education / Triticeae-CAP project (2011-68002-30029) of the US Department of Agriculture National Institute of Food and Agriculture / U.S. Wheat and Barley Scab Initiative (USWBSI)

Dry Pea or field pea (Pisum sativum L.) is an important cool season legume crop grown in the United States. Field peas are vulnerable to many diseases of which, soil borne diseases including wilt and root rot are of major economic importance and can cause significant reduction in yield. There is a dearth of satisfactory methods for control of root rot and no varieties with complete resistance to Fusarium root rot are currently available. Root rot disease was found to be prevalent in all the major pea growing counties of North Dakota surveyed in 2004, 2005, 2010 and 2011. Fusarium species were the most frequently isolated fungal species from the infected pea roots of which, F. oxysporum and F. avenaceum were the most common. 21 Field pea varieties were screened for resistance against F. avenaceum and F. solani f. sp. pisi, the Fusarium species traditionally associated with root rots of field pea in growth chamber experiments and field trials. Low levels of resistance were detected in a few cultivars but no variety was found to be completely resistant to any of the pathogens tested. Efficiency of precipitated calcium carbonate (PCC) in controlling Fusarium species most commonly associated with root rots was evaluated under in vitro and field conditions. Significant reduction in spore production, spore germination, and dry mycelial weight of Fusarium spp. were detected on PCC amended media in laboratory studies. In greenhouse and field experiments significant reduction in root rot disease severity was observed with PCC application compared to control. Fungal gene expression in artificially infected field pea roots and F. graminearum grown in culture was assessed using the Illumina mRNA-Seq technology. A total of 613 F. graminearum genes were found to be differentially expressed in planta on pea. Functional classes associated with amino acid metabolism, nitrogen metabolism, extracellular polysaccharide degradation, detoxification by degradation and defense related proteins were found to be significantly enriched in the up-regulated gene set as determined using FunCatDB. Expression of four up-regulated genes was confirmed by RT-PCR to validate the inferences from the sequencing results.

Air drying of naturally infested soils (0.12 to 0.38% water or about -2,000 bars) resulted in no recovery of Cylindrocladium crotalariae microsclerotia, but rewetting soils to near field capacity for 1 to 4 wk before assay resulted in partial recovery from the air drying-induced decreased germinability. Numbers of germinable C. crotalariae microsclerotia (assayed at 26 C) decreased progressively over 4 wk when naturally infested soils were incubated at 6 C. No germinable microsclerotia were recovered when soils were incubated at -10 C. When soils incubated at -10 C and at 6 C were transferred to 26 C for 4 wk, the low-temperature effect was partially reversed. Incubation of naturally infested soils under field conditions over the winter months (November-February) indicated that a similar low-temperature phenomenon exists in nature. Germinability of axenic, laboratory-grown microsclerotia of C. crotalariae, C. floridanum, and C. scoparium incubated 4 wk at 6 C ranged from 0 to 91.3% (mean = 37.7%) of the initial germinability. Partial recovery of laboratory-grown microsclerotia from the lowtemperature effect, by incubation at 26 C, was demonstrated. Conductivity measurements of solutions bathing microsclerotia incubated at 6 C and 26 C for 4 wk indicated that chilling injury may account, in part, for decreased germinability of microsclerotia. Direct observation of washed conidia of C. scoparium on rewetted, non-sterile soils at 26 C indicated that peak germination (33-58%) occurred after 24 h incubation. Peak germination on continually moist soils was somewhat lower (18-26%) than on rewetted soils. Conidia did not germinate on continually moist soils at 6 C. Conidia germinated at a high level (93-95%) in axenic culture in the absence of exogenous carbon and nitrogen substrates. The inhibition of conidium germination on soils was due, in part, to the presence of fungistatic soil volatiles. Addition of low levels of carbon and nitrogen substrates nullified the inhibitory effect of soil volatiles. Germinability of C. scoparium, C. crotalariae, and C. floridanum conidia in artificially infested soils (assayed on a selective medium at 26 C) decreased progressively during incubation at 26 C from 1 wk to 4 mo. No germinable conidia were recovered after incubation of soils at 6 C for 4 wk. Control of Cylindrocladium root rot of black wainut seedlings with sodium azide at 224 kg/ha applied by the plowdown method was comparable to MC-33 at 504 kg/ha. Sodium azide at 67 kg/ha was only marginally effective in disease control. Reduction of Cylindrocladium microsclerotium populations, qualitatively assayed by the azalea leaftrap method, was found in soil samples from plots fumigated with sodium azide and MC-33. A small-spored Cylindrocladium sp. with clavate to papillate vesicles, identified as C. parvum, was consistently recovered from diseased roots and soil samples from a Virginia forest nursery. Percentage recovery from necrotic roots and soil samples collected from root zones of necrotic black walnut seedlings was appreciably higher for C. parvum than for C. floridanum or C. scoparium. In greenhouse tests, limited pathogenicity of C. parvum on black walnut seedlings was demonstrated. / Ph. D.