"July 2001" / Includes bibliographical references (leaves 144-183) / xv, 193 leaves : ill., plates (col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Applied and Molecular Ecology, 2002

Concern about NO$ sb3 sp-$-N leaching and groundwater pollution from monoculture corn (Zea mays L.) has prompted investigation of alternative production systems which reduce N leaching. Both intercrop systems and water table controls alone have been shown to increase nitrogen (N) uptake and reduce soil NO$ sb3 sp-$-N accumulation in cropping systems. There is a need to maintain crop productivity while reducing the potential for soil NO$ sb3 sp-$-N leaching into groundwater. However, there has been no information available regarding agronomic and physiological aspects of N and water management for monocrop corn and corn competing with annual Italian ryegrass (Lolium multiflorum Lam) in an intercrop system. A study was conducted in southwestern Quebec during 1993 and 1994. Nitrogen and dry matter components in the plant-soil system were determined. Intercropped corn grain yield did not differ from monocropped corn under high N fertility. At harvest, the corn-annual ryegrass intercrop system increased total aboveground N uptake by 77.2 and 50.7 kg ha$ sp{-1}$ when compared with the corn monocrop system in 1993 and 1994, respectively. The intercrop system reduced the amount of NO$ sb3 sp-$-N in the top 1 m of soil by 47% (92.3 kg N ha$ sp{-1}$) at harvest in 1993. Water table controls had little effect on corn yield, N use efficiency and soil NO$ sb3 sp-$-N accumulation over the two years of this study. Both plant establishment and weather conditions affected the ability of annual ryegrass to aid in the uptake of soil NO$ sb3 sp-$-N. The reproductive development of water stressed plants after silking was limited more by overall plant changes due to water stress than assimilate supply. The delivery of C (sucrose) and N ($ sp{15}$N urea) into corn plants via stem-injection showed that externally supplied C changed both the source strength (photosynthetic inhibition) and sink strength (decreased total grain production), while distribution of $ sp{15}$N was affected by p

A fragment of the Claviceps purpurea pyr4 gene, encoding the enzyme orotidine-5'-monophosphate decarboxylase (OMPdecarboxylase) was used to screen a genomic library to an isolate (designated Lp1) of an Acremonium sp. which grows as an endophyte in perennial ryegrass (Lolium perenne). Four positive clones, λMC11, λMC12, λMC14 and λMC20 were isolated. Three of these clones, λMC12, λMC14 and λMC20 were overlapping clones from the same locus, while λMC11 was from a different locus. Fragments of these clones which hybridised with C. purpurea pyr4 were sequenced and found to have similarity with pyr4 from other fungi of the Pyrenomycetes and related Deuteromycetes, suggesting that Lp1 has evolved from a sexual Pyrenomycetes species. The pyr4 from λMC12, λMC14 and λMC20 was designated pyr4-1 and that from λMC11 was designated pyr4-2. The predicted ORFs of the two genes were highly conserved and the 5' non-coding nucleotide sequences were the least conserved regions. RT-PCR and northern analysis of total RNA from Lp1 demonstrated that transcripts approximately 1.4 kb in length were produced from the two genes and present at similar levels. Genomic fragments containing pyr4-1 or pyr4-2 were transformed into a strain of Aspergillus nidulans which has a mutation in the pyrG gene (encoding OMPdecarboxylase). Both of the Lp1 pyr4 complemented a pyrG mutation in Aspergillus nidulans, confirming that both pyr4-1 and pyr4-2 encode functional OMPdecarboxylases. Comparisons of pyr4 restriction fragment length polymorphisms (RFLPs) from Lp1 and isolates of Epichloë typhina, E. festucae, A. lolii, A. uncinatum, and three endophyte taxonomic groupings from Festuca arundinacea: FaTG-1 (=A. coenophialum), FaTG-2 and FaTG-3 suggested that pyr4-1 originated from E. typhina, the ryegrass choke pathogen, and pyr4-2 originated from A. lolii, another endophyte from perennial ryegrass. This suggested that Lp1 is an interspecific hybrid, between E. typhina and A. lolii. Comparisons of the variable 5' non-coding nucleotide sequences from pyr4 of Lp1 and other isolates demonstrated that E. typhina, and A. lolii or E. festucae were the most likely ancestors of the two pyr4 found in Lp1. The A. lolii and E. festucae sequences were very similar, suggesting they are closely related. A. lolii has most probably evolved from an E. festucae, and in the process lost the sexual cycle. Analysis of single spore purified isolates of Lp1 revealed that Lp1 was a homokaryon for pyr4. A Southern blot of a CHEF gel of Lp1 and these single spored isolates was hybridised to a pyr4 probe and demonstrated that pyr4-1 and pyr4-2 were present on either two chromosomes of similar size, or one chromosome. The hybridisation that gave rise to Lp1 was concluded to have been a relatively recent event, given the similarity of pyr4-1 and pyr4-2 nucleotide sequences to those of their probable ancestors, and the fact that both genes are expressed and functional. Interspecific hybridisation is probably widespread in the asexual endophytes, and may be an important event in their evolution, and the evolution of other fungal species.

No description available.

Thesis (PhD(Agric) (Agronomy))--University of Stellenbosch, 2005. / The response of perennial ryegrass and white clover, grown under controlled conditions, to fertiliser N rates applied under variable soil temperature (6, 12 and 18 °C), soil water potential (-10, -20, -25 and -35 kPa) and seasonal growing (June/July and October/November) conditions as well as field conditions, were evaluated. Primary- (PDM), residual- (RDM) and total dry matter (TDM) production (g pot-1) were recorded over the first- and second regrowth cycles as well as the accumulative DM production over the two regrowth cycles, respectively. Leaf N content (%) was recorded at the end of first and second regrowth cycles. Tiller/stolon numbers and root dry mass (g pot-1) were recorded at the end of the second regrowth cycle. Soil ammonium-N and nitrate-N (mg kg-1) content was monitored after fertiliser N application. Decreasing soil temperatures resulted in decreased TDM production in both crops. Only perennial ryegrass was influenced by fertiliser N rate, with a general increase in dry matter production as fertiliser N rate was increased. Ryegrass TDM production did not differ between the 100 and 150 kg N ha-1 rates but were both higher (P=0.05) if compared to the 0 and 50 kg N ha-1 treatments. Soil nitrate levels 31 days after application of 150 kg N ha–1 were still sufficient to stimulate ryegrass RDM production. The 173.8% increase in ryegrass TDM production measured at 6 °C where 150 kg N ha-1 was applied compared to the 0 kg N ha-1 treatment illustrated the ability of ryegrass to respond to fertiliser N at low soil temperatures. Soil water potential of -20 kPa resulted in higher ryegrass PDM and TDM production compared to the -25 and -35 kPa levels. White clover PDM and TDM production were however not influenced by soil water potential or fertiliser N rate. Ryegrass TDM production increased (P=0.05) as fertiliser N rates were increased. The most favourable soil water level for both ryegrass and clover root development was found to be -35 kPa. Perennial ryegrass and white clover PDM, RDM and TDM production were higher during the October/November season compared to the June/July season. Increased fertiliser N rates resulted in increased (P=0.05) ryegrass PDM and TDM production. White clover dry matter production was not influenced by fertiliser N rates. In the field study the effect of 0, 50, 100 and 150 kg N ha-1 applied as a single application either in autumn, early winter, late winter, early spring or late spring on pasture dry matter production, clover content and selected quality parameters of a perennial ryegrass-white clover pasture were investigated. Soil nitrogen dynamics in the 0-100, 200-300 and 400-500 mm soil layers were studied for 49 days following fertiliser N application. The effect of 50 kg N ha-1 on soil N dynamics was generally the same as found at the 0 kg N ha-1 applications and may therefore be regarded as a low risk treatment. The application of 150 kg N ha-1 especially in autumn and early winter showed a tendency to exceed the absorption capacity of the pasture and thereby expose fertiliser N to possible leaching and contamination of natural resources. Increased fertiliser N rate resulted in a general increase in pasture dry matter production with the highest yields recorded where N was applied in early and late spring and the lowest in early winter. The application of 150 kg N ha-1 in early and late spring resulted in the highest TDM production, however, the 50 kg N ha-1 resulted in a more efficient conversion of N applied to additional DM produced. In contrast to DM production, the clover percentage generally decreased as fertiliser N rate was increased. The effect of season of application was inconsistent. Annual trends show that the clover percentage eventually recovered to the same levels as the 0 kg N ha-1 treatments. Due to the above minimum levels recorded for most mineral and quality parameters tested it is envisaged that treatment combinations as used in this study will not be at any disadvantage to pasture and animal productivity. The study has shown that the use of fertiliser N to boost perennial ryegrass-white clover productivity and thereby minimising the negative effect of the winter gap on fodder flow management during the cool season in the Western Cape Province, may be an important management tool. Except for late spring applications, all seasons of application reduced the negative impact of the winter gap on fodder availability. It is concluded that regression lines as summarised in Tables 7.2 and 8.2 show great potential to be instrumental in developing regression models, accurately predicting the effect of fertiliser N rate on pasture performance. Other factors to be considered includes the productivity of the pasture, initial clover content, expected clover content at the end of the first regrowth cycle after fertiliser N application and the quantity of additional fodder required. Additional requirements will be to maintain and 150 kg N ha-1) in winter, as the N uptake capacity of the pasture could be exceeded and thereby increasing the risk of N leaching, resulting in environmental pollution. The N response efficiency of the pasture is also the lowest at the 150 kg N ha-1 rates, thereby reducing the profitability of these treatments.

Total organic carbon, total nitrogen, microbial respiration and enzyme activity (β-glucosidase) were measured on several horizons of a Dayton silt loam (fine, montmorillonitic, mesic, Typic Albaqualf) soil cropped to annual ryegrass under two straw residue management systems. The study evaluated the effects of annual burning of straw residues or annual incorporation of straw residues on the content, distribution and bioavailability of soil organic carbon. Four fields were selected to represent the burn management system which have been annually burned for a minimum of 40 years. Four fields were selected to represent the straw incorportated system (mold board plow) which had been annually burned for approximately 30 years, followed by incorporation of straw residues into soil for a minimum of 10 years. One native site was selected to represent non-cultivation conditions. Straw management system strongly influenced both the total organic C and N and microbial activity the surface soil horizon. Soil organic C and N content were significantly greater (p < 0.05) in the Ap horizons of soils under the plowed management system than soils under the burned management system. The collective evidence suggests, however, that the significant differences observed between the two residue management systems are not due to greater losses of soil organic C and N as a result of burning but rather that organic C levels have increased as a result of a change in management. Soil C:N ratios are slightly higher in the Ap horizons of soils as a result of straw residue incorporation in comparison to burning of straw residues. When expressed on a mass soil basis, both C0₂ evolution from microbial respiration, during a 32 day incubation period, and enzyme activity were significantly greater (p < 0.10) for the Ap horizon of soils where straw residue had been incorporated than in soils where residues had been annually burned. When expressed on a per gram C basis, neither C0₂ evolution from microbial respiration or enzyme activity were significantly different between the two management systems. These results indicate that long-term annual burning of straw residues has not decreased the bioavailability of soil organic C. / Graduation date: 1992

Thesis(M.S.)--Auburn University, 2005. / Abstract. Vita. Includes bibliographic references.