Faculty of Engineering and Built Enviroment School of Electrical and Information Engineering 0314356t morayus@engineer.com / A teleworking service allows people to work effectively together from home or other approved locations away from the regular work site, on an established work schedule. This is made possible via the use of Information and Communications Technology (ICT). Presently, there are isolated applications that can assist teleworkers, such as e-mail and video conferencing, which were developed for use over the Internet. But the Internet is a best-effort network with no guarantee of Quality of service (QoS), low security and no standard billing system. The design of this teleworking service involves the integration of many existing services like e-mail, messaging, video conferencing, shared whiteboard and database access. Other requirements are for service providers interworking for service and resource usage, security, and QoS specification. Hence, we explore the emerging open service concept to create this integrated teleworking service that can be made available for subscription by corporate bodies and individuals. Service federation is the interaction between teleworkers across service provider domains. It is achieved via the interworking of providers’ services, and is an essential aspect of teleworking. We have realised a service federation in a secure and seamless manner in the OSA / Parlay environment via the use of the OSA / Parlay framework. We looked at the use of a framework federation for the actual implementation of service federation. This framework federation is an interworking of frameworks based on an agreed-upon federation contract between them. New framework interfaces were introduced to facilitate this proposed solution, as the OSA / Parlay specifications do not yet support this approach. Service composition is the creation of a new service instance by composing one or more other services. We implemented this via the use of framework and trader federation. The trader federation was used to locate services or users in different ASP domains. A high level design of the teleworking service was done with federation explored for actual implementation. The Common Object Request Broker Architecture (CORBA) trading service was used to prove the concept. The RM-ODP methodology is followed in this teleworking service design. The OSA / Parlay terminal capability, generic call control, multiparty and location and Service Capability Features (SCF) were used for implementing in the CORBA Distributed Processing Environment (DPE).

No description available.

Student Number :9600874K - MSc research report - School of Electrical Engineering - Faculty of Engineering and the Built Environment / Telecommunication networks have evolved from voice only single service networks to multimedia networks providing bearer services such as voice, data and video transportation. Moreover, these networks, collectively called Next Generation Networks (NGNs), enable rapid creation, deployment and management of advanced services in an efficient manner. However, the initial business model of telcos was to internally develop and provide these advanced services to customers. In this monopolized environment, service development is driven by technological availability rather than customer demands. Furthermore, vendor specific network elements prohibit the development of re-useable service components, which in turn increases the time-to-market of services. Deregulation and advances in Distributed Computing Systems (DCSs) are driving towards open networks and rapid service delivery. Third party Application Service Providers (ASPs) are envisioned to develop and supply the services, with the telco providing bearer services. The use of softswitch architectures such as Open Service Access (OSA) / Parlay (OSA / Parlay) in an open NGN environment abstract services from core network elements through its Application Programming Interface (API). Services are thus decoupled from vendor and protocol specific network equipment and can be provided across a plethora of network architectures. One major advantage of NGN is the ability to provide bearer service in a mobile environment. Location Based Services (LBSs) are envisaged to be an important class of services provided in the NGN environment. For an LBS service to be complete, a geospatial database is necessary to provide location information. This report documents the design and implementation of a Geospatial Data Access Service Capability Feature (GDASCF) as an extension to the OSA / Parlay gateway. The GDASCF encapsulates necessary APIs that offer uniform access to query geospatial databases. One key component of the design is the realization of the Adapter layer which adapts function calls to an appropriate Database Management System (DBMS). The introduction of the GDASCF and Adapter layer provides a solution which results in flexible and rapid service creation.

L'ARN interférence est un mécanisme moléculaire conservé chez les Eucaryotes dont les principaux acteurs sont les protéines ARGONAUTE (AGO). Chez les plantes, AGO1 est une protéine essentielle à la croissance et la défense antivirale. Elle utilise des petits ARNs comme sondes pour reconnaître et réguler des ARN messagers. Les virus ont développé des suppresseurs de l'ARN interférence pour surmonter cette défense. L'un d'entre eux, P0 du virus de la mosaïque jaune du navet, est comme une protéine F-box qui détourne le complexe SCF, une ubiquitine ligase E3, et conduit AGO1 vers la protéolyse ubiquitine-dépendante. Cette dégradation utilise la vacuole au lieu du protéasome 26S, généralement associé à la dégradation ubiquitine-dépendante. Ce mécanisme de protéolyse n'est pas compris et est aussi apparent quand AGO1 est déstabilisé de manière endogène, suggérant que P0 utilise une voie déjà existante. Une protéine F-box d'Arabidopsis, FBW2, a été décrite comme impactant l'homéostasie d'AGO1 indépendamment du protéasome. Mon projet de thèse visait à caractériser l'activité F-box de FBW2 et à comprendre la relation entre AGO1 et FBW2 ainsi que ses conséquences sur l'ARN interférence. Les résultats obtenus dans ce manuscrit montrent que le complexe SCFFBW2 interagit avec AGO1 et déclenche sa dégradation via un processus indépendant de l'autophagie ou du protéasome, tout en n'affectant que faiblement l'ARN interférence. FBW2 ciblerait en fait un sous-ensemble de protéines AGO1 qui semble ne pas contenir de petits ARNs. Cette régulation jouerait un rôle de surveillance pour prévenir une activité délétère d'AGO1 en absence de petits ARNs. / RNA silencing is a conserved molecular mechanism in eukaryotes, of which the main effectors are the ARGONAUTE (AGO) proteins. In plants, AGO1 is a protein that is essential for growth and antiviral defence. It uses small RNAs as probe to recognize and regulate messenger RNAs. Viruses have developed suppressors of RNA silencing to overcome this defence. One of these, P0 from the Turnip Yellows Virus, acts as an F-box protein to hijack the SCF complex, an E3 ubiquitin ligase, and guide AGO1 to the ubiquitin-dependent proteolysis. This degradation uses the vacuole instead of the 26S proteasome, generally associated with ubiquitin-dependant proteolysis. This proteolysis mechanism is not understood and is also apparent when AGO1 is endogenously destabilized, suggesting that P0 uses an already existing pathway. An Arabidopsis F-box protein, FBW2, has been shown to impact AGO1 homeostasis independently from the proteasome. My PhD project aimed at characterizing FBW2 F-box activity and understanding the relationship between AGO1 and FBW2, as well as its consequences on the RNA silencing. The results obtained in this manuscript show that the SCFFBW2 interacts with AGO1 and triggers its degradation through an autophagy- and proteasome- independent process, while only weakly affecting the RNA silencing. FBW2 would actually target a subset of AGO1 proteins, which appears not to contain small RNAs. This regulation would play a surveillance role in order to prevent a deleterious activity of AGO1 in absence of small RNAs.

KIT receptor (CD117) is a receptor tyrosine kinase crucial for homeostasis of mast cells (MCs) in tissues and recruitment to sites of inflammation and tumors in response to its ligand Stem cell factor (SCF). Gain of function mutations in KIT (e.g. D816V) are frequently observed in systemic mastocytosis and other cancer types. Src Homology 2 domain containing phosphatase-2 (SHP2 or PTPN11) is a protein tyrosine phosphatase that promotes cell proliferation, survival and motility in multiple pathways and cell types. To study SHP2 function in MCs, we generated novel MC-specific Shp2 knock-out (KO) mice (MC-shp2 KO). These mice had reduced numbers of MCs in skin and peritoneum, and defective contact hypersensitivity responses compared to control mice, consistent with SHP2 promoting MC homeostasis. Using an inducible SHP2 KO bone marrow-derived MC (BMMC) culture model, we found that SHP2 KO cells were prone to apoptosis and had no MC repopulating activity in vivo. Mechanistically, SHP2 enhanced ERK activation and downregulation of pro-apoptotic protein Bim. SHP2 KO BMMCs also had defects in chemotaxis towards SCF, due to impaired activation of a Lyn/Vav/Rac pathway in SHP2 KO BMMCs. This correlated with defects in cell spreading, and F-actin polymerization in response to SCF. Treatment of BMMCs with a SHP2 inhibitor (II-B08) also led to reduced chemotaxis, consistent with SHP2 phosphatase activity being required for KIT-induced chemotaxis. Lastly, we tested whether SHP2 regulates oncogenic KIT signaling using a P815 mouse mastocytoma model. Stable silencing of SHP2 in P815 cells led to reduced cell growth and survival in vitro, and less aggressive systemic mastocytosis development in syngeneic mice. Overall, these studies identify SHP2 as a key node in SCF/KIT and oncogenic KIT pathways, and as a potential therapeutic target in several human diseases. / Thesis (Ph.D, Biochemistry) -- Queen's University, 2013-06-25 12:03:57.818

In this study we show, to our knowledge, the first description of the role ADAM10 plays on mast cells. ADAM10 is abundantly expressed on mast cells both in vitro and in vivo. Its expression is inhibited by IL-10, a suppressive cytokine. siRNA depletion of ADAM10 on bone marrow-derived mast cells (BMMC) caused decreased IL-6 production following IgE cross-linking and also impaired BMMC stem cell factor (SCF)-induced migration through collagen IV. Mast cells and T helper cells (Th cells) in the peritoneum were reduced in ADAM10 KO mice. In addition, ADAM10 KO BMMC produced significantly less of all cytokines measured following IgE cross-linking, including IL-6, TNF-α, IL-13, and MCP-1, compared to wild type BMMC. Collectively these data show that mast cell ADAM10 can be regulated by a T regulatory cell cytokine, IL-10, and describes key ways in which ADAM10 loss affects prototypical mast cell functions and distribution.

No description available.

This work uses different size of strain gages (Gage Grid Length: 10mm, 1mm, 0.2mm) to measure the stress distributions of metal (Al 6061-T6) and composite (laminates of [0/+45/90/-45]2S and [0/90]4S layers ) with a central circular hole and a crack. Both the SCF (Stress Concentration Factor) and SIF (Stress Intensity Factor) are found to consider the stress scatteredness of experimental data in contrast to micro- mechanics. To understand the strain gage size effect is our main purpose in measuring both types of materials. The size effect of strain gage we find appears obviously in the measuring position with stress changing significantly. We find that the size of strain gage is bigger; the error of stress is higher. Analyzing the stress scattering around a central circular hole, we obtain the experimental result that the stress will first reduce drastically from central circular hole edge and then keep uniform. In analysis of the stress scattering around the crack tip, we observe that first stress rises quickly from the specimen¡¦s free edge to the top of crack tip with a maximum value, and the stress keeps a nominal value in the specimen central part. Comparing the empirical result of SCF and SIF, we find that the data of 0.2mm of strain gage close to the theoretical solution, while those of 1mm and 10mm strain gages appear inexact in measuring and calculating SCF and SIF.

No description available.

Studying household finance and behavior is important not only for understanding the micro level behavior, but also to have a better understanding of the whole economy. The study of household behavior become even more important after the 2007 housing crises, and the giant effect it had (and is still having) on Wall Street and the whole US and global economy. This dissertation is an attempt to understand some of the household behavior: how does the new internet era affect the demand for credit cards, which attitudes influence the household's decision to file bankruptcy, and whether expenditure habits encouraged overspending. The study will use the micro level data provides by Survey of Consumer Finance SCF2007. In the first chapter, the study focuses on the effect of having internet access on household demand for credit cards, controlling for standard price, income effects and other financial and demographic variables. The internet changed the way consumer shops, with more information about the product, the market and the price. E-Commerce retail sales grew on average of 22% a year over the past decade; in the second quarter of 2009 it reached $32.4 billion. The study found that households who have access to the internet, carry around $862 more on their credit card balance, in average, than households who have no access to the internet. The second chapter investigates the effects of borrowing and saving attitudes on household decision to file for personal bankruptcy. The total non-commercial bankruptcy filings increased from 560,682 cases in 2006 to 784,079 in 2007. This increase continued through 2008 and the first quarter of 2009, with 1,031,443 cases filed in 2008 and 304,228 in the first three months of 2009. The study results suggest that borrowing and saving attitudes have no effect of household decision to file for bankruptcy except for paying credit card balance in full every month. The third chapter studies the relationship between eating out "Food-Away-From-Home" and overspending. Since 19% of household in the US are spending more than their income. The average for the past nine years (2000-2008) was 1.6%. Compared to other industrialized countries, the US had one of the worst personal saving rates during the past twenty years . The study found that eating out does not encourage overspending. On the contrary, the higher the ratio of FAFH to total food expenditures the less likely household will overspend.