Information science and technology fast development not only impact industry external competition environment, but also has the significant influence to the enterprise interior management. Along with network technology maturity, more and more enterprises Internet technical application in enterprise, thus constructs the construction enterprise exclusively intranet system, to unimpeded internal information, and provides each kind of convenient service and the function, then reduces the cost, to strengthened enterprise physique, to ensure the competitive advantage. On this occasion of the information explodes, no matter the society or individual, the lifelong study is the inevitable tendency, but constructs the construction using the enterprise interior network e-Learning to provide the multiplex study pipeline, may cause the knowledge management and study obtains more convenient and effectiveness. Steps while the whole world to the information, the science and technology and the government advocated the lifelong study, the enterprise promote the industry to encourage education, personally to take advanced courses become common practice, it reveal e-Learning potential to become the epoch-making education learning. However, from the view point of business management, lead-in e-Learning system incurs great expense. This research use the¡uUnified Theory of Acceptance and Use of Technology / UTAUT¡vas a foundation, from the large-scale traditional industry as a viewpoint, revises its scales, and use the way of lamination random sampling to carry on the questionnaire survey to the case study company, and obtains 180 effectively questionnaires. The main conclusion includes: To the case study company's staffs [1] Influence e-Learning system to accept a factor to be allowed to induce is "Performance Expectancy ", ¡§Effort Expectancy ", " Social Influence ¡§and " Facilitating Conditions " four items, different staff have different factor value degree. [2] The influence system use wish factor only has ¡§Performance Expectancy ", and " Facilitating Conditions " and so on two items; At the same time directly the behavior has the influence " Performance Expectancy " to "Actual use": The other two factors influence (Effort Expectancy and Social Influence) certainly does not reveal. [3] " Performance Influence" for the wish of uses and the actual behavior influence far surpass other factors.