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Functional characterization of GPI-anchored proteins of the SKU5/SKS gene family / Caractérisation fonctionnelle des protéines à ancre GPI de la famille des gènes SKU5/SKSZhou, Ke 21 June 2013 (has links)
ABP1 (Auxin Binding Protein 1), qui peut se lier à l'auxine, est essentielle pour le développement des plantes. Il a été prouvé qu’elle a la capacité de se lier à l’auxine et de conduire le signal auxine dans les cellules. ABP1 est supposé être localisée et avoir des fonctions à la surface extérieure de la membrane plasmique à travers une composante inconnue. Au cours ma thèse, nous avons essayé d’étudier l'interaction entre ABP1 et le candidat de la composante inconnue, CBP1 (chez le maïs), qui est une protéine à ancres GPI déjà identifiée comme ayant la capacité de liaison au peptide de synthèse C-terminale d’ABP1 en 2006. L'orthologue de CBP1 chez arabidopsis appartient à une famille de gènes contenant 19 membres, dont seulement trois d'entre eux ont été prédit comme était des protéines à ancres GPI. Nous avons fait les caractérisations fonctionnelles de ces trois membres. Les données suggèrent que les protéines SKS à ancres GPI sont impliquées dans l'orientation de la cellule, le développement des gamétophytes et de l'embryon. / ABP1 (Auxin Binding Protein1), who can bind auxin, is essential for the development of plants. It was proved to have the ability to bind auxin and transduce auxin signal into the cells. It is supposed to be localized and functions at the outer surface of plasma membrane through unknown component. In my thesis, we tried to invesitgate the interaction between ABP1 and the candidate of the unknown component, CBP1 (From maize), which is GPI-acnhored and already identified as the binding ability to synthesized C-terminus peptide of ABP1 in 2006. The orthologous of CBP1 in arabidopsis belongs to a gene family with 19 members, in which only three of them were prediceted to be GPI anchored. We did the functional characterisation of these three GPI-anchored members. Data suggested that GPI-anchored SKS were involved in cell orientation, gametophyte and embryo development.
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Functional characterization of GPI-anchored proteins of the SKU5/SKS gene familyZhou, Ke 21 June 2013 (has links) (PDF)
ABP1 (Auxin Binding Protein1), who can bind auxin, is essential for the development of plants. It was proved to have the ability to bind auxin and transduce auxin signal into the cells. It is supposed to be localized and functions at the outer surface of plasma membrane through unknown component. In my thesis, we tried to invesitgate the interaction between ABP1 and the candidate of the unknown component, CBP1 (From maize), which is GPI-acnhored and already identified as the binding ability to synthesized C-terminus peptide of ABP1 in 2006. The orthologous of CBP1 in arabidopsis belongs to a gene family with 19 members, in which only three of them were prediceted to be GPI anchored. We did the functional characterisation of these three GPI-anchored members. Data suggested that GPI-anchored SKS were involved in cell orientation, gametophyte and embryo development.
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