A propaganda eleitoral e especificamente o Horário Gratuito de Propaganda Eleitoral (HGPE) são temas de numerosa quantidade de trabalhos no Brasil, mas poucos consideram analisar os spots eleitorais e mais raro ainda é o estudo de seu impacto sobre o voto. Considerando essa lacuna na literatura, esta tese visa entender como os diferentes tipos de spot eleitorais de TV (positivo, negativo e comparativo) afetam a decisão do voto no Brasil. Para tanto, o caso estudado se restringe à análise da eleição presidencial de 2010, tendo como objeto empírico os spots veiculados pelos dois principais candidatos da disputa: Dilma Rousseff e José Serra. Com base no modelo de inteligência afetiva (MARCUS; MCKUEN; NEUMAN, 2000) e uma vasta literatura dos efeitos da comunicação política sobre o voto (BRADER, 2007; TINKHAM; WEAVER-LARISCY,1993; JOHNSON-CARTEE; COPELAND,1991; MERRITT, 1984; PINKLETON, 1997), foi realizado um estudo experimental para avaliar como esses diferentes tipos de spot interferiram na escolha dos eleitores. Dentre os resultados, observa-se que os spots positivos foram eficientes em aumentar a vontade de votar nos candidatos do spot, mas pouco eficientes para diminuir a vontade de votar no candidato adversário. Em segundo lugar, verificou-se que os spots comparativos tiveram impacto mais parecido aos spots positivos do que aos negativos para a tomada de decisão do voto, o que contraria a literatura sobre o tema, que considera esse tipo de spot como negativo. Observou-se também que os spots negativos não apresentaram efeito bumerangue, o que denota grande capacidade estratégica desse tipo de spot para minar a intenção de voto do adversário sem que isso signifique necessariamente perdas para o candidato que o veicula. / The electoral propaganda and specifically the Free Time Electoral Propaganda (HGPE) on TV are the subject of a great amount of works in Brazil, but few of them consider analyze the electoral spots and rarer still is the study of its impact on the vote. Given that gap in the literature, this thesis aims to understand how the different types of electoral TV spots (positive, negative and comparative) affect the vote decision in Brazil. Therefore, the case study is restricted to the analysis of the presidential election of 2010, having as empirical object the spots served by the two main candidates of the dispute: Dilma Rousseff and José Serra. Based on the emotional intelligence model (Marcus; McKuen; Neuman, 2000) and a vast literature of the effects of political communication on the vote (Brader, 2007; Tinkham; Weaver-Lariscy, 1993; Johnson-Cartee; Copeland, 1991; Merritt 1984; Pinkleton, 1997 etc.), an experimental study was carried out to evaluate how these different types of spot influenced the choice of the voters. Among the results, it is observed that the positive spots were effective in increasing the intention to vote in the candidate of the spot, but not efficient to reduce the intention to vote in the opponent candidate. Second, the comparative spots were more like impact to the positive spots than negative ones to influence the decision making of the vote, which is contrary to the literature on the subject, which considers such spot as negative. It was also observed that negative spots showed no boomerang effect, which shows great strategic capacity of this type of spot to undermine the intent of the opponent\'s vote without necessarily mean losses for the candidate that conveys.

O objetivo desse trabalho é apresentar o desenvolvimento de modelos numéricos baseados no método dos elementos finitos, para investigar o comportamento termoelástico, de um freio a tambor utilizado em veículos pesados. O acoplamento de fenômenos físicos e mecânicos durante o processo de frenagem é uma etapa fundamental para o projeto com foco em confiabilidade. A identificação de falhas potenciais decorrentes dos diferentes processos de frenagens, ainda na fase do projeto preliminar, permite o desenvolvimento de projetos mais robustos, incluindo reduções de tempo e nos custos de ensaios experimentais. Este trabalho apresenta o desenvolvimento e implementação de um modelo computacional baseado no comportamento termoelástico dos principais elementos mecânicos de um freio a tambor envolvidos no processo de frenagem. Foram consideradas três condições principais de análise: estática de corpos rígidos, estática de corpos flexíveis e dinâmica em regime permanente com corpos flexíveis. O objetivo é investigar e estimar valores de pressão de contato, cargas resultantes nos apoios, bem como obter o gradiente de temperatura e tensão devido à carga térmica em regiões de interesse. Essa análise pode melhorar o entendimento dos fenômenos envolvidos nos diferentes processos de frenagens, auxiliando as tomadas de decisões técnicas, principalmente na antecipação de falhas prematuras relacionadas com fadiga térmica e desgaste adesivo. / This work presents the development of a numerical model based on thermoelastic behavior of a drum brake applied to heavy vehicles. The coupling of physics and mechanical phenomena during the braking process represents an important phase in the design vehicle systems. The identification of potential failures during the braking processes, considering the preliminary design phase gives robustness to the design. This characteristic allows reducing the time and cost of experimental set-up. This work presents the development and preliminary results of a numerical model based on thermal-elastic behavior of an automotive drum brake. It was considered three main conditions for the analysis: rigid bodies statics, flexible bodies statics and flexible bodies steady state dynamic analysis. The main objective of this study is investigate and estimate the relationship between the design parameters, as strain, stress as well thermal loads and temperature gradient, in conditions of static and quasi-static steady state regime. The estimative of these parameters to support the technical decisions related to reliability design, mainly to anticipate failures arising to thermal fatigue and adhesive wear.

Cette thèse en économie de l'énergie et de l'environnement étend le modèle de Hotelling du type exploration-extraction avec contraintes géologiques d’Okullo, Reynes et Hofkes (2015), afin de prendre en compte des trajectoires en forme de cloche pour l’ajout de réserves empiriquement observées par Laherrère (2003). Le modèle LOGIMA proposé (Images à Long terme sur le Pétrole et le Gaz) explique qu’elles sont la conséquence de « sweet spots » géologiques : des zones privilégiées où la concentration d’hydrocarbures est la plus élevée. Le modèle LOGIMA a été calibré sur une base de données issues couvrant les sept principaux bassins de pétrole et de gaz non-conventionnels du pays. Les résultats indiquent que la nécessité d’apprentissage par la pratique pour découvrir l’emplacement des sweet spots conduit à une mise en œuvre d’un effort d’exploration également en forme de cloche, ce qui permet de réduire le risque des activités d’exploration. Par conséquent, la réponse en termes des volumes offerts par les producteurs à des chocs sur les prix dévient fonction de l’ensemble des ressources mondiales antérieurement découvertes. Ensuite, nous appliquons le modèle LOGIMA pour étudier l’impact causé par l’offre de pétrole et de gaz naturel « non-conventionnels » aux États-Unis, sur les efforts mondiaux d’atténuation du changement climatique. Nous y parvenons en associant les scénarios à long-terme générés par LOGIMA avec le modèle d’évaluation intégrée, IMACLIM-R. Cette étude analyse comment des différentes cibles de prix de pétrole affecteraient son offre aux États-Unis. Nous estimons cette interaction au moyen de trois cadres de politiques en matière de climat : le cadre « business as usual » (BAU), les contributions décidées à l’échelle nationale (NDC) et les scénarios de 2°C (2DS). Les résultats de l’exercice indiquent que les approvisionnements non-conventionnels sont fortement susceptibles d’affecter les marchés énergétiques mondiaux, mais leur impact sur les émissions mondiales de gaz à effet de serre serait limité, car les différents effets déclenchés dans des différents secteurs viendraient les équilibrer approximativement. / This thesis in energy and environmental economics extends the geological Hotelling-type extraction-exploration model from Okullo, Reynes and Hofkes (2015) in order to account for the bell-shaped reserve additions that were empirically observed by Laherrère (2003). The proposed model explains them as the result of geological “sweet spots”: premium areas within geological formations where the concentration of hydrocarbons is highest. The proposed theoretical formulation was programmed into the mathematical model LOGIMA – “Long-term Oil and Gas Images” – and calibrated on data covering the seven main unconventional oil and gas plays in the United States. Results indicate the need to learn the location of sweet spots through trial and error drillings leads to schedules of exploratory effort that allow the optimal “de-risking” of exploratory activities. As a result, the optimal response of producers to price shocks becomes contingent on the prevailing level of cumulative discoveries.We apply LOGIMA to investigate the impact, caused by the recent advent of large-scale supplies of unconventional oil and gas, in the United States, on the ongoing efforts to mitigate climate change. We do so by soft coupling long-term scenarios from LOGIMA with the integrated assessment model, IMACLIM-R, a recursive, computable general equilibrium model of integrated global energy, economy and environment systems. We analyze how different price targets, potentially pursued by the Organization of Petroleum Exporting Countries (OPEC), would affect supplies of unconventional oil and gas from the United States. We control this interplay under three climate policy frameworks: business as usual (BAU), nationally determined contributions (NDCs) and 2°C scenario (2DS). The results of the exercise show that, despite having a significant potential to affect global energy markets, unconventional oil and gas supplies would have a limited potential to affect global cumulative greenhouse gas emissions to 2040, as the different effects triggered in different sectors approximately balanced each other out.

A síntese e caracterização de sistemas hetero-híbridos gerados a partir da 3,6-bi-2-piridil-1,2,4,5-tetrazina (bptz) e interações com ouro nanoparticulado são abordados nesta tese. O bptz foi estudado por meio de métodos espectroscópicos e teóricos, focalizando principalmente o efeito SERS associado à adsorção em nanopartículas de ouro. O mecanismo de transferência de carga para metais macroscópicos foi transposto para a condição nanoparticulada, envolvendo ligações químicas entre bptz e as nanopartículas. Os complexos estudados possuem fórmula geral [LmRu(µ-bptz)RuLm]Xn, com “L” indicando os ligantes periféricos 5-cloro-1,10-fenantrolina (Clphen) ou 4’-(fenil)-2,2&#8217:6&#8217,2&#8221-terpirdina (ptpy) e “X” os contra-íons. Foram investigadas suas espectroeletroquímicas eletrônica e SERS, e as mudanças de perfil vibracional foram modeladas, incorporando o mecanismo de transferências de carga entre complexo e o ouro, além dos mecanismos ressonantes e eletromagnéticos / The synthesis and investigation of heterohybrid systems encompassing 3,6-bi-2-pyridyl-1,2,4,5-tetrazine (bptz) and its ruthenium complexes associated with gold nanoparticles are dealt with in this thesis. Bptz was characterized by spectroscopic and theoretical techniques, focusing on its SERS spectra after the adsorption onto nanoparticles. The charge transfer mechanism in the SERS spectra of macroscopic metals was transposed to the nanoparticle condition, assuming the formation of chemical bonds between bptz and the nanoparticles. Complexes of general formula [LmRu(µ--bptz)RuLm]Xn, “L” the peripheric ligants 5-chlorine-1,10-phenantroline or 4’-(phenyl)-2,2&#8217:6&#8217,2&#8221-terpyrdine (ptpy), and “X” counter-ions were also investigated, with special emphasis on their electronic and SERS spectroelectrochemistry. The changes in the vibrational profiles were successfully explained by the occurrence of charge transfer between the adsorbed complex and gold, in addition to the electromagnetic and resonance mechanisms.

No description available.

A propaganda eleitoral e especificamente o Horário Gratuito de Propaganda Eleitoral (HGPE) são temas de numerosa quantidade de trabalhos no Brasil, mas poucos consideram analisar os spots eleitorais e mais raro ainda é o estudo de seu impacto sobre o voto. Considerando essa lacuna na literatura, esta tese visa entender como os diferentes tipos de spot eleitorais de TV (positivo, negativo e comparativo) afetam a decisão do voto no Brasil. Para tanto, o caso estudado se restringe à análise da eleição presidencial de 2010, tendo como objeto empírico os spots veiculados pelos dois principais candidatos da disputa: Dilma Rousseff e José Serra. Com base no modelo de inteligência afetiva (MARCUS; MCKUEN; NEUMAN, 2000) e uma vasta literatura dos efeitos da comunicação política sobre o voto (BRADER, 2007; TINKHAM; WEAVER-LARISCY,1993; JOHNSON-CARTEE; COPELAND,1991; MERRITT, 1984; PINKLETON, 1997), foi realizado um estudo experimental para avaliar como esses diferentes tipos de spot interferiram na escolha dos eleitores. Dentre os resultados, observa-se que os spots positivos foram eficientes em aumentar a vontade de votar nos candidatos do spot, mas pouco eficientes para diminuir a vontade de votar no candidato adversário. Em segundo lugar, verificou-se que os spots comparativos tiveram impacto mais parecido aos spots positivos do que aos negativos para a tomada de decisão do voto, o que contraria a literatura sobre o tema, que considera esse tipo de spot como negativo. Observou-se também que os spots negativos não apresentaram efeito bumerangue, o que denota grande capacidade estratégica desse tipo de spot para minar a intenção de voto do adversário sem que isso signifique necessariamente perdas para o candidato que o veicula. / The electoral propaganda and specifically the Free Time Electoral Propaganda (HGPE) on TV are the subject of a great amount of works in Brazil, but few of them consider analyze the electoral spots and rarer still is the study of its impact on the vote. Given that gap in the literature, this thesis aims to understand how the different types of electoral TV spots (positive, negative and comparative) affect the vote decision in Brazil. Therefore, the case study is restricted to the analysis of the presidential election of 2010, having as empirical object the spots served by the two main candidates of the dispute: Dilma Rousseff and José Serra. Based on the emotional intelligence model (Marcus; McKuen; Neuman, 2000) and a vast literature of the effects of political communication on the vote (Brader, 2007; Tinkham; Weaver-Lariscy, 1993; Johnson-Cartee; Copeland, 1991; Merritt 1984; Pinkleton, 1997 etc.), an experimental study was carried out to evaluate how these different types of spot influenced the choice of the voters. Among the results, it is observed that the positive spots were effective in increasing the intention to vote in the candidate of the spot, but not efficient to reduce the intention to vote in the opponent candidate. Second, the comparative spots were more like impact to the positive spots than negative ones to influence the decision making of the vote, which is contrary to the literature on the subject, which considers such spot as negative. It was also observed that negative spots showed no boomerang effect, which shows great strategic capacity of this type of spot to undermine the intent of the opponent\'s vote without necessarily mean losses for the candidate that conveys.

La performance des cibles du Laser LMJ est très sensible à l'uniformité de la tache focale du laser. La technique utilisée à ce jour pour éviter les inhomogénéités est de rendre le faisceau partiellement incohérent, ce qui a pour conséquence la fluctuation de points chauds (speckles) au cours du temps, avec un effet de moyenne. De nombreux travaux ont d'ores et déjà été menés concernant l'étude de ces points chauds dans le cadre d'un faisceau unique au plan focal. Dans cette thèse, on s'intéresse à ces points chauds lorsqu'il sont issus de la superposition de faisceaux multiples, et plus particulièrement aux points chauds dans le cas de la configuration du LMJ. On a établi une équation analytique permettant de calculer la taille des points chauds dans le cas d'un speckle généré par des faisceaux multiples. Cette formule est en parfait accord avec les différents résultats obtenus par simulations numériques grâce au code PARAX. On a ainsi pu prédire la taille du grain de speckle LMJ en fonction de différentes configurations LMJ telle qu'une configuration à deux ou trois cônes et pour des choix de polarisations différents. Il en ressort que l'impact de la polarisation est assez faible sur les caractéristiques du speckle LMJ. Toutefois la double polarisation est la plus adaptée aux critères d'uniformité requis pour une bonne interaction laser-plasma. D'autre part on a montré que les grains de speckle LMJ sont de l'ordre de la longueur d'onde.Dans la seconde partie de cette thèse, on a établi les formules des contraste, temps de vie, trajectoires et vitesses des points chauds deux techniques de lissage : Lissage par Dispersion Spectrale Transverse (LDST) et Longitudinal (LDSL). Ces derniers sont respectivement utilisés sur le NIF et prochainement sur le LMJ. On a ainsi montré que quels que soient le nombre de cône et le choix des polarisations faites sur le LMJ, la durée de vie d'un point chaud LMJ est environ de 2 ps et le contraste intégré sur des temps infinis est approximativement de 15%. Les vitesses sont également très faibles (10-6c) et faiblement influencées par le choix des polarisations. / The future French Laser Megajoule (LMJ) is a multiple laser beam facility built to achieve Inertial confinement Fusion (ICF). It is mainly designed for an indirect drive scheme using X-rays conversion. In this scheme, the target in the center of the hohlraum is irradiated and then compressed by the X-rays more uniformly than what it would be in a direct drive schemes. However, a high of uniformity is still needed to reach ignition since the propagation of intense laser beams in an under-critical plasma can generate laser-plasma instabilities (LPI). The control of LPI is of crucial importance for the success of ICF. By breaking both spatial and temporal coherences, the use of optical smoothing techniques, such as smoothing by spectral dispersion (SSD), often dramatically reduces LPI and also ensures the reproducibility of laser conditions from one shot to another. An accurate description of the speckle pattern in the hohlraum is thus of great interest for ICF experiments.We focus our attention on the spatial and then temporal properties of the speckles pattern generated by multiple laser beams. At first, we establish equations for the 3D speckle size based on autocorrelation functions. Numerical simulations of the propagation of multiple laser beams in vacuum are then performed with the PARAX code in configurations where the paraxial approximation can be used. The case of speckle patterns in the LMJ configuration in the zone where all the beams overlap is eventually studied. We show that such speckles have an ellipsoidal shape. Finally, influence of the polarization of the beams on the shape, size and abundance of the speckles is also investigated. In a second part we study the important aspect of temporal smoothing techniques like the movement of the speckles. This work is also triggered by the development of a statistical model that describes the motion of hot spots in order to evaluate the contrast, the trajectory and the velocity of LMJ hot spots. We address these quantities in the case of a speckle pattern generated by multiple laser beams thanks to the autocorrelation function in intensity.

Thesis (PhD)--Stellenbosch University, 2000. / ENGLISH ABSTRACT: Gray leaf spot (GLS) of maize, caused by the fungus Cercospora zeae-maydis, can reduce grain yields by up to 60% and it is now recognized as one of the most significant yield-limiting diseases of maize in many parts of the world. The most sustainable and long-term management strategy for GLS will rely heavily on the development of high-yielding, locally adapted GLS resistant hybrids. Molecular markers could be useful to plant breeders to indirectly select for genes affecting GLS resistance and to identify resistance genes without inoculation and at an early stage of plant development. Only two studies in the USA have examined quantitative trait loci (QTL) association with GLS resistance. The aim of this study was to map GLS resistance genes in a resistant Seed Co LTD, Zimbabwean inbred line. Molecular markers linked to the GLS resistance QTL were identified by using the amplified fragment length polymorphism (AFLP) technique together with bulked segregant analysis. Eleven polymorphic AFLP fragments were identified and converted to sequence-specific PCR (polymerase chain reaction) markers. Eight of the 11 converted AFLP markers were added to the maize marker database of the University of Stellenbosch. Five of the 8 converted AFLP markers were polymorphic between the resistant and the susceptible parent. They were amplified on the DNA of 230 plants of a segregating F2 population and linkage analysis was performed with MAPMAKER/EXP. Two linkage groups consisting of two markers each, with a linkage distance of 10.4 cM (LOD 22.83) and 8.2 cM (LOD 55.41) between the two markers, were identified. QTL mapping with MAPMAKER/QTL confirmed the presence of QTL in both linkage groups. Two publicly available recombinant inbred families (Burr et a/., 1988) were used to localize the converted AFLP markers on the genetic map of maize. The QTL, which were identified with the AFLP markers, were mapped to chromosomes 1 and 5. Another AFLP marker was mapped to chromosome 2 and a further to chromosome 3. To obtain more precise localizations of the QTL on chromosomes 1 and 5, sequence-tagged site markers and microsatellite markers were used. The markers were amplified on the DNA of the 230 plants of the F2 population and linkage analysis was performed with MAPMAKER/EXP. The order of the markers was in agreement with the UMC map of the Maize Genome Database. Interval mapping using MAPMAKERlQTL and composite interval mapping using QTL Cartographer were performed. The QTL on chromosome 1 had a LOD score of 21 and was localized in bin 1.05/06. A variance of 37% was explained by the QTL. Two peaks were visible for the QTL on chromosome 5, one was localized in bin 5.03/04 and the other in bin 5.05/06. Both peaks had a LOD score of 5 and 11% of the variance was explained by the QTL. To test the consistency of the detected QTL, the markers flanking each QTL were amplified on selected plants of two F2 populations planted in consecutive years and regression analysis was performed. Both the QTL on chromosome 1 and the QTL on chromosome 5 were detected in these populations. Furthermore, the presence of a QTL on chromosome 3 was confirmed with these populations. A variance of 8 -10% was explained by the QTL on chromosome 3. In this study, a major GLS resistance QTL was thus mapped on chromosomes 1 and two minor GLS resistance QTL were mapped on chromosomes 3 and 5 using a resistant Seed Co LTD, Zimbabwean inbred line. Markers were identified which could be used in a marker-assisted selection program to select for the GLS resistance QTL. / AFRIKAANSE OPSOMMING: Grys blaarvlek (GBV) van mielies, veroorsaak deur die swam Cercospora zeaemaydis, kan graanopbrengs met tot 60% verlaag en word beskou as een van die vernaamste opbrengs-beperkende siektes wêreldwyd. Die toepaslikste langtermyn stragtegie vir GBV beheer sal wees om plaaslike mieliebasters met hoë opbrengs en GBV weerstand te ontwikkel. Molekulêre merkers kan nuttig deur plantetelers gebruik word om weerstandsgene te selekteer. Seleksie is moontlik in die afwesigheid van inokolum en op 'n vroeë stadium van plant ontwikkeling. Slegs twee vorige studies (in die VSA) het kwantitatiewe-kenmerk-Iokusse (KKL), vir GBVweerstand ondersoek. Die doel van hierdie studie was om die GBV weerstandsgene in 'n weerstandbiedende ingeteelde lyn (Seed Co BPK, Zimbabwe) te karteer. Molekulêre merkers gekoppel aan die GBV weerstands KKL is geïdentifiseer deur gebruik te maak van die geamplifiseerde-fragmentlengte-polimorfisme- (AFLP-) tegniek en gebulkte-segregaat-analise. Elf polimorfiese merkers is geïdentifiseer en omgeskakel na volgorde-spesifieke PKR (polimerase kettingreaksie) merkers. Agt van die elf omgeskakelde AFLP-merkers is by die mieliemerker databasis van die Universiteit van Stellenbosch gevoeg. Vyf van die 8 omgeskakelde AFLP-merkers was polimorfies tussen die bestande en vatbare ouers. Hulle is geamplifiseer op die DNA van 230 plante van 'n segregerende F2-populasie en is gebruik in 'n koppelingstudie met MAPMAKER/EXP. Twee koppelingsgroepe, elk bestaande uit twee merkers, met onderskeidelik koppelingsafstande van 10.4 eM (LOD 22.83) en 8.2 eM (LOD 55.41) tussen die merkers, is geïdentifiseer. KKL-kartering het getoon dat KKL in albei koppelingsgroepe aanwesig is. Twee kommersieël beskikbare, rekombinant-ingeteelde families (Burr et aI., 1988) is gebruik om die omgeskakelde AFLP-merkers op die mielie genetiese kaart te plaas. Die KKL wat met die AFLP-merkers geïdentifiseer is, is gekarteer op chromosome 1 en 5. 'n Verdere AFLP-merker is op chromosoom 2 gekarteer en 'n ander op chromosoom 3. Ten einde die KKL op chromosome 1 en 5 meer akkuraat te karteer, is volgordege- etikeerde en mikrosatelliet merkers gebruik. Die merkers is geamplifiseer op die DNA van die 230 plante van die F2-populasie en koppelings-analises is uitgevoer. Die volgorde van die merkers was dieselfde as die van die UMC-kaart in die Mielie Genoom Databasis. Interval kartering met MAPMAKER/QTL en komposiet interval kartering met QTL Cartographer is uitgevoer. Die KKL op chromosoom 1 het 'n LOD-telling van 21 gehad en is in bin 1.05/06 geplaas. Die KKL was verantwoordelik vir 37% van die variansie. Twee pieke was onderskeibaar vir die KKL op chromosoom 5, een in bin 5.03/04 geleë en die ander in bin 5.05/06. Elke piek het 'n LOD-telling van 5 gehad en die twee KKL was verantwoordelik vir 11% van die variansie. Om die herhaalbaarheid van die effek van die KKL te toets is die merkers naaste aan elke KKL geamplifiseer op geselekteerde plante van twee F2-populasies wat in opeenvolgende jare geplant is. Regressie analise is op die data gedoen. Beide die KKL op chromosoom 1 en die KKL op chromosoom 5 kon in hierdie populasies geïdentifiseer word. Verder kon die aanwesigheid van 'n verdere KKL op chromosoom 3 in hierdie populasies bevestig word. Laasgenoemde KKL was verantwoordelik vir 8-10% van die totale variansie. In hierdie studie is daar dus 'n hoof GBV-weerstands KKL gekarteer op chromosoom 1 en twee kleiner GBV-weerstands KKL gekarteer op chromosome 3 en 5. Merkers is geïdentifiseer wat moontlik in merker-gebaseerdetelingsprogramme gebruik kan word om plante te selekteer wat die GBVweerstands KKL het.

This written thesis accompanies three television public service announcement spots. Two of the spots are 60 seconds and one of the spots is 45 seconds in length. I produced this public service television advertising campaign to highlight the issue of child illiteracy in Pakistan and to encourage expatriate and resident Pakistani's to donate to educational charities. A Website created by the filmmaker is promoted in the campaign. This Website provides information about various charities that educate children in Pakistan. Detailed accounts of pre-production, production and post-production of the campaign allow the viewer to comprehend the challenges in producing television campaigns for social causes. Theoretical issues are also discussed, including the causes of illiteracy, the importance and role of social campaigns, the history and uses of propaganda as well as the aesthetic concerns of a public service campaign producer. I discuss the importance of creating the culture of public service campaigns in a third world country like Pakistan, and states that the Pakistani community needs to look inwards to overcome the challenge of illiteracy.

Energy sorrel (Rumex patientia L. x Rumex tianschanicus A. Los.) is a plant with a high yield of above-ground biomass. Because of this, it is grown as energy crop, but in Ukraine it is also important forage crop. Mycobiota has not been examined, even though energy sorrel is grown in the Czech Republic experimentally since 1992 (in agricultural operations since 2001). The aims of this thesis were: to determine the composition of mycobiota of healthy plants and of mycobiota associated with leaf and stem spots, to verify whether fungi isolated from spots can cause these spots and if previously isolated fungus Phomopsis sp. can cause spots. Samples were colected from 4 sites in Czech republic, in three samplings during the vegetation season. Endophytes were isolated from root, stem and leaf. Fungi asociated with spots were isolated from stem and leaf. Fungi were cultivated on 2% malt extract agar and then morphologically determined. From representative strains were isolated DNA and sequences of the ITS region were compared with the GenBank databases, this verified the morphological determination and the sterile species were determined. The most common endophytes were Clonostachys rosea f. rosea, Cladosporium cladosporioides, Rhizopus arrhizus var. arrhizus, Alternaria alternata a Acremonium strictum...