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Efficacious Combination Drug Treatment for Colorectal Cancer that Overcomes Resistance to KRAS G12C Inhibitors / KRAS G12C阻害薬耐性の大腸癌に対する有効な併用療法の開発Matsubara, Hiroyuki 23 May 2023 (has links)
京都大学 / 新制・課程博士 / 博士(医学) / 甲第24804号 / 医博第4996号 / 新制||医||1067(附属図書館) / 京都大学大学院医学研究科医学専攻 / (主査)教授 武藤 学, 教授 藤田 恭之, 教授 川口 義弥 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM
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Interactions of Cells with Magnetic Nanowires and Micro NeedlesPerez, Jose E. 12 1900 (has links)
The use of nanowires, nano and micro needles in biomedical applications has
markedly increased in the past years, mainly due to attractive properties such as
biocompatibility and simple fabrication. Specifically, these structures have shown
promise in applications including cell separation, tumor cell capture, intracellular
delivery, cell therapy, cancer treatment and as cell growth scaffolds.
The work proposed here aims to study two platforms for different applications:
a vertical magnetic nanowire array for mesenchymal stem cell differentiation and a
micro needle platform for intracellular delivery.
First, a thorough evaluation of the cytotoxicity of nanowires was done in order
to understand how a biological system interacts with high aspect ratio structures.
Nanowires were fabricated through pulsed electrodeposition and characterized by
electron microscopy, vibrating sample magnetometry and energy dispersive X-ray
spectroscopy. Studies of biocompatibility, cell death, cell membrane integrity,
nanowire internalization and intracellular dissolution were all performed in order
to characterize the cell response. Results showed a variable biocompatibility
depending on nanowire concentration and incubation time, with cell death resulting
from an apoptotic pathway arising after internalization.
A vertical array of nanowires was then used as a scaffold for the differentiation
of human mesenchymal stem cells. Using fluorescence and electron microscopy, the
interactions between the dense array of nanowires and the cells were analyzed, as
well as the biocompatibility of the array and its effects on cell differentiation. A
magnetic field was additionally applied on the substrate to observe a possible
differentiation. Stem cells grown on this scaffold showed a cytoskeleton and focal
adhesion reorganization, and later expressed the osteogenic marker osteopontin.
The application of a magnetic field counteracted this outcome.
Lastly, a micro needle platform was fabricated through lithography and
electrodeposition, characterized using the previously mentioned techniques and
then evaluated as a vector for intracellular delivery. Fluorescence and electron
microscopy imaging were first performed to assess the biocompatibility, cell
spreading and the interface of the cells and the needles. Intracellular delivery of a
fluorescent dye was achieved via inductive heating of the needles, with the results
showing a dependency of delivery and cell survivability on the exposure time.
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Role of Amyloid Precursor Protein in Neuroregeneration on an In Vitro Model in Alzheimer's Patient-Specific Cell LinesBedoya Martinez, Lina S 01 January 2019 (has links)
Alzheimer's disease (AD) leads to neurodegeneration resulting in cognitive and physical impairments. AD is denoted by accumulation of intracellular neurofibrillary tangles, known as tau, and extracellular plaques of the amyloid beta protein (Aβ). Aβ results from the proteolytic cleavage of the amyloid precursor protein (APP) by β- and gamma-secretases in the amyloidogenic pathway. Although, Aβ has been widely studied for neurodegeneration, the role of APP in both, the healthy and diseased conditions, has not yet been entirely understood. The function that APP has in neural stem cell (NSC) proliferation, differentiation, and migration during adult neurogenesis has been previously studied. Additionally, APP has be shown to be overexpressed after neural damage resulted from conditions, such as AD and traumatic brain injury (TBI). In this study, the role of APP in in vitro damaged neural tissue cells was further investigated by evaluating neural progenitor cell proliferation, migration, and differentiation after a scratch assay. For these purposes, induced pluripotent stem (iPS) cells from AD patients were differentiated into neural progenitor cells to model the disease conditions and later treated with Phenserine to reduce their levels of APP expression. The results suggested that APP may enhance neural progenitor cell proliferation and glial differentiation while inhibiting neural progenitor cell migration and neuronal cell specialization after neural tissue damage.
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Retroviral-mediated gene transduction of bone marrow-derived stem cellsAllay, James Andre January 1996 (has links)
No description available.
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Dual-Gene Transfer and Vector Targeting for Hematopoietic Stem Cell Gene TherapyRoth, Justin Charles January 2006 (has links)
No description available.
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Cell-Taught Gene Therapy for the Preservation and Regeneration of Cardiac Tissue Following Chronic Heart FailureSundararaman, Srividya 05 January 2011 (has links)
No description available.
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Testing for Osteogenic Potential of Human Mesenchymal Stem CellsLause, Gregory E. 23 August 2011 (has links)
No description available.
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Determining the Roles of Novel Genes in Neuroblast Development and DifferentiationStopczynski, Nathan 12 June 2014 (has links)
No description available.
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EVALUATING THE ROLE OF BREAST CANCER STEM CELL POPULATIONS AS PREDICTORS OF RESPONSE TO TRASTUZUMAB TREATMENTSandoval, Maria Luisa 02 September 2014 (has links)
No description available.
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Mechanisms of endoderm patterning and directed differentiation of human stem cells into foregut tissuesMcCracken, Kyle W. 18 September 2014 (has links)
No description available.
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